Porcine circovirus associated diseases diagnosis at industrial pig farm conditions

2019 ◽  
Vol 333 (10) ◽  
pp. 30-32
Author(s):  
S.A. Raev ◽  
◽  
A.G. Yushakov ◽  
K.P. Alekseev ◽  
M. Anoyatbekov ◽  
...  
Keyword(s):  
Porcine Circovirus ◽  
Associated Diseases ◽  
Pig Farm

Application of immunohistochemistry method in diagnosis of porcine circovirus associated diseases

2019 ◽  
Vol 22 (8) ◽  
pp. 18-22
Author(s):  
V.V. Stafford ◽  
◽  
E.B. Streltsova ◽  
S.A. Raev ◽  
A.G. Yushakov ◽  
...  
Keyword(s):  
Porcine Circovirus ◽  
Associated Diseases

Low Molecular Weight Sulfated Chitosan Efficiently Reduces Infection Capacity of Porcine Circovirus Type 2 (PCV2) In PK15 Cells

2021 ◽  
Author(s):  
Daniela Jiménez-Arriagada ◽  
Alejandro A. Hidalgo ◽  
Victor Neira ◽  
Andrónico Neira-Carrillo ◽  
Sergio A. Bucarey
Keyword(s):  
Molecular Weight ◽  
Antiviral Activity ◽  
Mode Of Action ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus Type 2 ◽  
Pcv2 Infection ◽  
Porcine Circovirus ◽  
Molecular Weights ◽  
Associated Diseases

Abstract Background Porcine circovirus type 2 (PCV2)-associated diseases are a major problem for the swine industry worldwide. In addition to vaccines, the availability of antiviral polymers provides an efficient and safe option for reducing the impact of these diseases. By virtue of their molecular weight and repetitious structure, polymers possess properties not found in small-molecule drugs. In this perspective, we focus on chitosan, a ubiquitous biopolymer, that adjusts the molecular weight and sulfated-mediated functionality could act as a efficient antiviral polymer by mimicking PCV2-cell receptor interactions. Methods Sulfated chitosan (Chi-S) polymers of two molecular weights were synthesized and characterized by FTIR, SEM-EDS and elemental analysis. The Chi-S solutions were tested against PCV2 infection in PK15 cells in vitro and antiviral activity was evaluated by measuring the PCV2 copy number upon application different molecular weights, sulfate functionalization, and concentration of polymer. In addition, to explore the mode of action of the Chi-S against PCV2 infection, experiments were designed to clarify whether the antiviral activity of the Chi-S would be influenced by when it was added to the cells, relative to the time and stage of viral infection. Results Chi-S significantly reduced genomic copies of PCV2, showing specific antiviral effects depending on its molecular weight, concentration, and chemical functionalization. Assays designed to explore the mode of action of Chi-S revealed that exerted antiviral activity through impeding viral attachment and penetration into cells. Conclusions These findings help better understanding PCV2-porcine cells interaction and reinforce the idea that sulfated polymers, such as Chi-S, represent a promising candidate for uses in antiviral therapies against PCV2-associated diseases.

Porcine circovirus type 3 detection in a Hungarian pig farm experiencing reproductive failures

2019 ◽  
Vol 185 (3) ◽  
pp. 84-84 ◽  
Author(s):  
Zoltán Deim ◽  
László Dencső ◽  
Ildikó Erdélyi ◽  
Sarshad Koderi Valappil ◽  
Csaba Varga ◽  
...  
Keyword(s):  
Porcine Circovirus Type ◽  
Reproductive Failure ◽  
Porcine Circovirus ◽  
Tissue Tropism ◽  
Viral Dna ◽  
Real Time Quantitative Pcr ◽  
Sequence Identity ◽  
Relevant Role ◽  
Pig Farm ◽  
Type 3

Porcine circovirus 3 (PCV3) infection has been reported in piglets and sows with porcine dermatitis and nephropathy syndrome, reproductive failure, and cardiac and multisystemic inflammation. Few studies linked PCV3 infection to increased incidence of abortion and weak-born piglets. This is the first report of a detection of PCV3 Hungarian strain in several organs of aborted and weak-born piglets, including the thymus, lymph node, placenta, spleen, kidney and the liver. The tissue tropism of PCV3 in affected litters was analysed using real-time quantitative PCR, and the result showed the highest load of viral DNA in the thymus and lymph nodes. The ORF2 of Hungarian PCV3 strains was 524 nucleotides in length, and the sequence identity to GenBank sequences ranged from 98.5 per cent to 99.2 per cent. The results suggest that PCV3 may have a relevant role in reproductive failure in gilts.

scholarly journals Detection and molecular characterization of porcine circovirus type 2 from piglets with Porcine Circovirus Associated Diseases in Colombia

2014 ◽  
Vol 11 (1) ◽  
Author(s):  
Maria Antonia Rincón Monroy ◽  
Gloria Consuelo Ramirez-Nieto ◽  
Victor Julio Vera ◽  
Jairo Jaime Correa ◽  
Jose Dario Mogollón-Galvis
Keyword(s):  
Molecular Characterization ◽  
Porcine Circovirus Type ◽  
Porcine Circovirus Type 2 ◽  
Porcine Circovirus ◽  
Associated Diseases

Porcine circovirus type 2 and associated diseases in Romania — Short communication

2007 ◽  
Vol 55 (1) ◽  
pp. 151-156 ◽  
Author(s):  
D. Cadar ◽  
A. Cságola ◽  
Á. Dán ◽  
Z. Deim ◽  
Marina Spînu ◽  
...  
Keyword(s):  
Clinical Signs ◽  
Porcine Circovirus Type ◽  
Postweaning Multisystemic Wasting Syndrome ◽  
Porcine Circovirus Type 2 ◽  
Porcine Circovirus ◽  
Serum Samples ◽  
Associated Diseases ◽  
Wasting Syndrome

Porcine circovirus type 2 (PCV2) has been demonstrated to be the causal agent for postweaning multisystemic wasting syndrome (PMWS) and porcine dermatitis and nephropathy syndrome (PDNS). This report describes the first detection of PCV2 and associated diseases in a Romanian swine herd located in Transylvania. The clinical signs, pathological and histopathological changes observed in affected pigs were similar to those previously described for PDNS and PMWS. Polymerase chain reaction and in situ hybridisation were used for the detection of PCV2 nucleic acids from tissues and serum samples. Complete PCV2 genomes of both PMWS and PDNS cases were sequenced and analysed, and by comparing them with each other no genomic differences could be detected. The sequence analysis showed that the Romanian PCV2 was closely related to PCV2 identified in France and in Hungary.

scholarly journals Global Status ofPorcine circovirusType 2 and Its Associated Diseases in Sub-Saharan Africa

2017 ◽  
Vol 2017 ◽  
pp. 1-16 ◽  
Author(s):  
Kayode O. Afolabi ◽  
Benson C. Iweriebor ◽  
Anthony I. Okoh ◽  
Larry C. Obi
Keyword(s):  
Economic Value ◽  
Economic Loss ◽  
Postweaning Multisystemic Wasting Syndrome ◽  
Sub Saharan Africa ◽  
Porcine Circovirus ◽  
African Region ◽  
Viral Pathogen ◽  
Associated Diseases ◽  
Wasting Syndrome ◽  
Sub Saharan

Globally,Porcine circovirustype 2 (PCV2) is a recognized viral pathogen of great economic value in pig farming. It is the major cause of ravaging postweaning multisystemic wasting syndrome (PMWS) and many other disease syndromes generally regarded asPorcine circovirusassociated diseases (PCVAD) in Europe. PCV2 infections, specifically PMWS, had impacted huge economic loss on swine production at different regions of the world. It has been studied and reported at different parts of the globe including: North and South America, Europe, Asia, Oceania, Middle East, and the Caribbean. However, till date, this virus and its associated diseases have been grossly understudied in sub-Sahara African region and the entire continent at large. Two out of forty-nine, representing just about 4% of countries that make up sub-Sahara Africa presently, have limited records on reported cases and occurrence of the viral pathogen despite the ubiquitous nature of the virus. This review presents an overview of the discovery ofPorcine circovirusand its associated diseases in global pig herds and emphasizes the latest trends in PCV2 vaccines and antiviral drugs development and the information gaps that exist on the occurrence of this important viral pathogen in swine herds of sub-Saharan Africa countries. This will serve as wake-up call for immediate and relevant actions by stakeholders in the region.

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