scholarly journals Next-generation sequencing technology in relation to our understanding and tackling of Antimicrobial Resistance [Tecnología de secuenciación de próxima generación en relación con nuestra comprensión y lucha contra la resistencia a los antimicrobianos]

2019 ◽  
Vol 3 (1) ◽  
pp. 1
Author(s):  
Roxana Guillen

Sequencing technologies have suffered over the last few years improvements in its performance, Next-generation Sequencing is being used more frequently to control infectious diseases, to know and anticipate antimicrobial resistance (AMR) and in surveillance controls against possible infectious outbreaks. Molecular assays used to detect pathogenic or antibiotic resistant agents take a lot of time and effort, and often enough information is not collected to make decisions. Next- generation sequencing appears to elucidate in the least time possible the whole DNA sequence and provide us with enough data to know resistance, virulence and typing that can be analyzed and a great help in research and decision making. NGS is a very promising technology, in order for it to be used extensively, requires the development of data analysis platforms and reduction of trials costs that still is very high for a massive use.

Weed Science ◽  
2009 ◽  
Vol 57 (5) ◽  
pp. 463-469 ◽  
Author(s):  
Ryan M. Lee ◽  
Jyothi Thimmapuram ◽  
Kate A. Thinglum ◽  
George Gong ◽  
Alvaro G. Hernandez ◽  
...  

Recent advances in sequencing technologies (next-generation sequencing) offer dramatically increased sequencing throughput at a lower cost than traditional Sanger sequencing. This technology is changing genomics research by allowing large scale sequencing experiments in nonmodel systems. Waterhemp is an important weed in the midwestern United States with characteristics that makes it an interesting ecological model. However, very few genomic resources are available for this species. One half of a 70 by 75 picotiter plate of 454-pyrosequencing was performed on total DNA isolated from waterhemp, generating 158,015 reads of an average length of 271 bp, or a total of nearly 43 Mbp of sequence. Included in this sequence was a nearly complete sequence of the chloroplast genome, sequences of several important herbicide resistance genes, leads for simple sequence repeat (SSR) markers, and a sampling of the repeated elements (e.g., transposons) present in this species. Here we present the waterhemp genomic data gleaned from this sequencing experiment and illustrate the value of next-generation sequencing technology to weed science research.


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