2. Identificación morfológica y molecular de cepas del hongo (Alternaria dauci) patógeno de la zanahoria (Daucus carota L.)

Adan Acevedo Cruz

doi:10.32911/as.2018.v11.n1.453

2. Identificación morfológica y molecular de cepas del hongo (Alternaria dauci) patógeno de la zanahoria (Daucus carota L.)

APORTE SANTIAGUINO ◽

10.32911/as.2018.v11.n1.453 ◽

2018 ◽

Vol 11 (1) ◽

pp. 21

Author(s):

Adan Acevedo Cruz

Keyword(s):

Daucus Carota ◽

Daucus Carota L ◽

Alternaria Dauci

La finalidad del estudio fue la identificación morfológica y molecular de las cepas de Alternaria dauci, causantes de la quemadura foliar de la zanahoria. Para ello, mediante técnicas convencionales se aislaron 50 cepas del hongo del cultivo de zanahoria, ubicada en el departamento de Landes al sudoeste de Francia. Las observaciones macroscópicas y microscópicas de las colonias mostraron que: 10 (42A, 42B, 25A, 37B, 22A, 12B, 2A, 22B, 12A y 25B) de las 50 cepas presentaron micelios de aspecto algodonoso, pigmentación rojo claro a marrón del Extracto de Malta Agar (EMA), conidióforos alargados y no ramificados, conidios ovalados con septos transversales y longitudinales. <br />Se realizó el monosporage de las 10 cepas con características propias de A. dauci se obtuvieron 40 cepas puras. De las cuales, la identificación molecular con la PCR, se logró amplificar el ADN de 30 cepas con los primers universales ITS1/ITS4 con una talla de 600 pb, y la verificación de estas cepas con la PCR utilizando los primers específicos Ada3 y Ada5 de A. dauci, reveló que 9 cepas puras: M25AA1, M12BA1, M12AA2, M37BA2, M22BB2, M25BB2, M42AB1, M42BB1 y M42BB2 pertenecen a la especie A. dauci con un peso molecular correspondiente a 159 pb.

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Effect of different inoculum levels of Meloidogyne incognita, Alternaria dauci and Rhizoctonia solani on the growth, chlorophyll and carotenoid content and disease progression of carrot (Daucus carota L.)

Acta Phytopathologica et Entomologica Hungarica ◽

10.1556/038.54.2019.025 ◽

2019 ◽

Vol 54 (2) ◽

pp. 211-220

Author(s):

L. Ahmad ◽

Z. A. Siddiqui

Keyword(s):

Rhizoctonia Solani ◽

Disease Progression ◽

Daucus Carota ◽

Meloidogyne Incognita ◽

Carotenoid Content ◽

Daucus Carota L ◽

Alternaria Dauci

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DAYA TERIMA JAJANAN LOKAL BARONCONG DENGAN PENAMBAHAN WORTEL (DAUCUS CAROTA L)

Media Gizi Pangan ◽

10.32382/mgp.v26i1.993 ◽

2019 ◽

Vol 26 (1) ◽

pp. 1

Author(s):

Suriani Rauf ◽

Manjilala Manjilala ◽

Elvi Kusuma

Keyword(s):

Experimental Study ◽

Daucus Carota ◽

Vegetable Consumption ◽

Negative Impact ◽

Local Foods ◽

Ministry Of Health ◽

Daucus Carota L

The behavior of people who less consume vegetables and fruit can have a negative impact on health. The lack of appetite for vegetable consumption by the society made the researchers interested in making a combination of vegetables, namely carrots with local foods, namely Baroncong.This research aims to determine the acceptability of local baroncong snacks with the addition of carrots.This research is a pre-experimental study. The panelists from this study consisted of 30 Nutrition Department Students from Health Polytechnic Ministry of Health Makassar. Assessment based on taste aspects, color of aroma and texture of baroncong products with the addition of carrots 50%, 75%, 100% with the criteria of very like, like, dislike and very dislike.The results showed that the acceptability of baroncong with the addition of carrots in terms of taste was a concentration of 75%, which was very much as much as 46.7% and liked as much as 50%, the color aspect was 50% concentration consisting of 36.7% likes and likes as much as 53.3%, the aroma aspectis a concentration of 50% which consists of very much as much as 33.3% and likes as much as 63.3%, in terms of aspects of texture are concentrations of 50% and 75%, each of which consists of as much as 23.3% and likes as much as 63, 3%. Based on these data, Baroncong with the addition of carrots received is a concentration of 75%.

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