Effect of different inoculum levels of Meloidogyne incognita, Alternaria dauci and Rhizoctonia solani on the growth, chlorophyll and carotenoid content and disease progression of carrot (Daucus carota L.)

2019 ◽  
Vol 54 (2) ◽  
pp. 211-220
Author(s):  
L. Ahmad ◽  
Z. A. Siddiqui
Keyword(s):  
Rhizoctonia Solani ◽  
Disease Progression ◽  
Daucus Carota ◽  
Meloidogyne Incognita ◽  
Carotenoid Content ◽  
Daucus Carota L ◽  
Alternaria Dauci

scholarly journals 2. Identificación morfológica y molecular de cepas del hongo (Alternaria dauci) patógeno de la zanahoria (Daucus carota L.)

2018 ◽  
Vol 11 (1) ◽  
pp. 21
Author(s):  
Adan Acevedo Cruz
Keyword(s):  
Daucus Carota ◽  
Daucus Carota L ◽  
Alternaria Dauci

La finalidad del estudio fue la identificación morfológica y molecular de las cepas de Alternaria dauci, causantes de la quemadura foliar de la zanahoria. Para ello, mediante técnicas convencionales se aislaron 50 cepas del hongo del cultivo de zanahoria, ubicada en el departamento de Landes al sudoeste de Francia. Las observaciones macroscópicas y microscópicas de las colonias mostraron que: 10 (42A, 42B, 25A, 37B, 22A, 12B, 2A, 22B, 12A y 25B) de las 50 cepas presentaron micelios de aspecto algodonoso, pigmentación rojo claro a marrón del Extracto de Malta Agar (EMA), conidióforos alargados y no ramificados, conidios ovalados con septos transversales y longitudinales. <br />Se realizó el monosporage de las 10 cepas con características propias de A. dauci se obtuvieron 40 cepas puras. De las cuales, la identificación molecular con la PCR, se logró amplificar el ADN de 30 cepas con los primers universales ITS1/ITS4 con una talla de 600 pb, y la verificación de estas cepas con la PCR utilizando los primers específicos Ada3 y Ada5 de A. dauci, reveló que 9 cepas puras: M25AA1, M12BA1, M12AA2, M37BA2, M22BB2, M25BB2, M42AB1, M42BB1 y M42BB2 pertenecen a la especie A. dauci con un peso molecular correspondiente a 159 pb.

scholarly journals 034 Independence of the Reduced Pigment (rp) Gene and the Y and Y2 Loci in Carrot (Daucus carota L.)

HortScience ◽  
1999 ◽  
Vol 34 (3) ◽  
pp. 447A-447
Author(s):  
Azumi Tozuka ◽  
N. Breitback ◽  
I.L. Goldman
Keyword(s):  
Daucus Carota ◽  
Carotenoid Content ◽  
Gene Model ◽  
Carrot Root ◽  
Daucus Carota L ◽  
Segregation Data ◽  
Data Support ◽  
Separate Locus ◽  
Yellow Orange ◽  
Pigment Biosynthesis

Five genes, including alleles at Y, Y1 and Y2, and the alleles I and IO, have been implicated in conditioning carrot root color. Various combinations of these alleles can condition white, yellow, and orange xylem and phloem color in carrot roots. The recently discovered reduced pigment gene, designated rp, conditions pale orange xylem and phloem and reduces carotenoid content by 92%. To determine if the rp gene is an allele of a previously described locus or a separate locus involved in pigment biosynthesis, we crossed rprp plants to plants with yellow phloem and yellow xylem (yellow/yellow) and to plants with purple phloem and yellow xylem (purple/yellow). We generated BC1 and F2 progenies for evaluation of segregation data. The expected genotype of plants with yellow xylem was yyY2Y2RPRP and the expected genotype of plants carrying rp was yyy2y2rprp. More than 1900 individual plants were evaluated for root color in 38 matings. In F2 progenies resulting from crosses of rprp × yellow/yellow, segregation data from 19 out of 20 families fit expected 12:3:1 (yellow: orange: rprp) or 15:1 (orange: non-orange) ratios (P < 0.001) indicating independence of the rp gene. In addition, 12 out of 16 families of F2 progenies from the cross between purple/yellow plants and rprp plants fit expected 12:3:1 (yellow: orange: rprp) ratios (P < 0.001) for a 2-gene model. These data support the hypothesis that the rp gene is independent and not linked to Y and Y2 genes.

scholarly journals Metodologias de inoculação de Rhizoctonia solani na cultura da cenoura

2008 ◽  
Vol 32 (3) ◽  
pp. 992-995
Author(s):  
Amanda Cabral Corrêa de Oliveira ◽  
Paulo Estevão de Souza ◽  
Edson Ampélio Pozza ◽  
Felipe de Carvalho Manerba ◽  
Maurício Ferreira Lopes
Keyword(s):  
Rhizoctonia Solani ◽  
Daucus Carota ◽  
Daucus Carota L

Rhizoctonia solani pode causar diferentes tipos de doenças em cenoura (Daucus carota L.). Para a avaliação de métodos de controle geralmente se utiliza inoculação artificial. Objetivou-se neste trabalho, ajustar uma concentração de inóculo de R. solani (AG-4) no cultivo de cenoura. Utilizou-se delineamento experimental em blocos ao acaso, com 5 repetições sendo a unidade experimental um vaso de 3L com 40 sementes. Como substrato, utilizou-se solo/areia (3:1). Os tratamentos em esquema fatorial 4 x 3 sendo, 4 densidades de inóculo (9; 18; 36; 72; mg de inóculo·kg-1 de solo) e 3 métodos de infestação artificial (incorporados a todo o solo; incorporados na superfície; contato direto com as sementes) e uma testemunha adicional. O experimento foi conduzido em câmara de crescimento a 20(0)C, com fotoperíodo de 12 h. As avaliações foram realizadas diariamente do 8º ao 30º dia após a semeadura, registrando-se o estande e o número de plântulas com tombamento. Analisou-se o índice de velocidade de emergência, porcentagem média de tombamento pré e pós emergência. A densidade de 72 mg de inóculo·kg-1 de solo incorporado na superfície foi o método mais eficiente.

scholarly journals The genes crucial to carotenoid metabolism under elevated CO2 levels in carrot (Daucus carota L.)

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Hongxia Song ◽  
Qiang Lu ◽  
Leiping Hou ◽  
Meilan Li
Keyword(s):  
Molecular Mechanism ◽  
Daucus Carota ◽  
Carotenoid Biosynthesis ◽  
Practical Significance ◽  
Carotenoid Content ◽  
Rna Seq ◽  
Daucus Carota L ◽  
Carotenoid Metabolism ◽  
Biological Yield ◽  
Missed Opportunity

AbstractThe CO2 saturation point can reach as high as 1819 μmol· mol−1 in carrot (Daucus carota L.). In recent years, carrot has been cultivated in out-of-season greenhouses, but the molecular mechanism of CO2 enrichment has been ignored, and this is a missed opportunity to gain a comprehensive understanding of this important process. In this study, it was found that CO2 enrichment increased the aboveground and belowground biomasses and greatly increased the carotenoid contents. Twenty genes related to carotenoids were discovered in 482 differentially expressed genes (DEGs) through RNA sequencing (RNA-Seq.). These genes were involved in either carotenoid biosynthesis or the composition of the photosystem membrane proteins, most of which were upregulated. We suspected that these genes were directly related to quality improvement and increases in biomass under CO2 enrichment in carrot. As such, β-carotene hydroxylase activity in carotenoid metabolism and the expression levels of coded genes were determined and analysed, and the results were consistent with the observed change in carotenoid content. These results illustrate the molecular mechanism by which the increase in carotenoid content after CO2 enrichment leads to the improvement of quality and biological yield. Our findings have important theoretical and practical significance.

Underwater shockwave pretreatment process for improving carotenoid content and yield of extracted carrot ( Daucus carota L.) juice

2017 ◽  
Vol 211 ◽  
pp. 15-21 ◽  
Author(s):  
Atsushi Yasuda ◽  
Eisuke Kuraya ◽  
Akiko Touyama ◽  
Osamu Higa ◽  
Kazuyuki Hokamoto ◽  
...  
Keyword(s):  
Daucus Carota ◽  
Carotenoid Content ◽  
Daucus Carota L

scholarly journals 440 A Comparative Study on Flavor and Nutritional Attributes of `BetaSweet' Maroon Carrot (Daucus carota L.)

HortScience ◽  
1999 ◽  
Vol 34 (3) ◽  
pp. 520C-520 ◽  
Author(s):  
Carlos A. Lazcano ◽  
L.M. Pike ◽  
K.S. Yoo
Keyword(s):  
Comparative Study ◽  
Daucus Carota ◽  
Reducing Sugars ◽  
Soluble Solids ◽  
Carotenoid Content ◽  
Fresh Weight ◽  
Daucus Carota L ◽  
Significant Difference ◽  
Volatile Terpenoids ◽  
Low Levels

A new designer carrot, `BetaSweet', with high levels of anthocyanin, betacarotene, and crispy texture was developed by the Vegetable Improvement Center at Texas A&M Univ. The new carrot contained low levels of low-volatile terpenoids, responsible for the harsh flavor in carrots and high levels of reducing sugars. Carotenoid content increased with carrot maturity and stabilize at 120 days after sowing for orange and maroon genotypes; however, the maroon genotype was 35% higher than the orange cultivar. Anthocyanin, a cancer preventive compound and not detected in ordinary orange carrots, is present in `BetaSweet' maroon carrot with 89.8 mg·100 g-1 of fresh weight. High percentage of soluble solids and succulence in the maroon cultivar seemed to contribute to the favored sweetness perception by consumers. A consumer taste panel showed a significant difference between orange and maroon genotype for sweetness, texture, and overall carrot flavor.

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