Selective sensitivity of the Black Sea microalgae to viral infection after exposure to a constant magnetic field

The change in sensitivity to viral infection of cultures of three species of Black Sea microalgae (Tetraselmis viridis, Dunaliella viridis and Phaeodactylum tricornutum) after exposure to a constant unidirectional magnetic field with a magnetic induction of 600 G is experimentally studied. The studies were carried out with using a developed laboratory installation. With the duration of the experiments 24 h and 48 h, it was recorded that being in a magnetic field resulted in an increase in the resistance to viral lysis in microalgae T. viridis, its decrease in P. tricornutum and no changes in D. viridis. We assume that the revealed changes in the sensitivity of microalgae to viral infection after exposure to a magnetic field are due to their species characteristics and selectivity.

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Effect of Viral Infection on the Functioning and Lysis of Black Sea Microalgae Tetraselmis viridis (Chlorophyta) and Phaeodactylum tricornutum (Bacillariophyta)

Inland Water Biology ◽

10.1134/s1995082920020303 ◽

2020 ◽

Vol 13 (3) ◽

pp. 417-424 ◽

Cited By ~ 1

Author(s):

L. V. Stelmakh ◽

O. A. Stepanova

Keyword(s):

Viral Infection ◽

Black Sea ◽

Phaeodactylum Tricornutum ◽

Tetraselmis Viridis

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FUNCTIONAL STATE OF THE MARINE MICROALGAE CULTURES AS AN INDICATOR OF THE WATER POLLUTION LEVEL OF THE SEVASTOPOL BAY

Monitoring systems of environment ◽

10.33075/2220-5861-2021-4-83-90 ◽

2021 ◽

pp. 83-90

Author(s):

L.V. Stelmakh ◽

◽

I.M. Mansurova ◽

Keyword(s):

Functional State ◽

Black Sea ◽

Phaeodactylum Tricornutum ◽

Inhibitory Effect ◽

The Black Sea ◽

Pollution Level ◽

Sevastopol Bay ◽

Algae Growth ◽

Taxonomic Affiliation

The possibility of using cultures of Black Sea planktonic microalgae of different taxonomic affiliation to assess the quality of the aquatic environment based on the assessment of their functional state is shown. The research was carried out on the diatom Phaeodactylum tricornutum, three dinoflagellates species Prorocentrum cordatum, Prorocentrum pusillum and Gyrodinium fissum, as well as the coccolithopho-ride Emiliania huxleyi. It was found that the waters of the Sevastopol Bay in the period from May to August 2021 in 50% of cases had a weak inhibitory effect on the growth of the tested species. In other cases, algae growth was either stimulated or the effect of pollution on cultures was not revealed. The need for using several types of algae in carrying out complex work on water biotesting of the coastal are-as of the Black Sea is highlighted.

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Growth of Tetraselmis viridis (Rouchijajnen) R.E.Norris, Hori & Chihara 1980 in the greenhouse pool under natural light and aeration

Issues of modern algology (Вопросы современной альгологии) ◽

10.33624/2311-0147-2019-3(21)-76-87 ◽

2019 ◽

pp. 76-87 ◽

Cited By ~ 2

Author(s):

Yana D. Zhondareva ◽

Rudolf P. Trenkenshu

Keyword(s):

Carbon Dioxide ◽

Experimental Evidence ◽

Batch Culture ◽

Black Sea ◽

Dry Weight ◽

Dry Mass ◽

The Black Sea ◽

Natural Light ◽

Carbon Supply ◽

Tetraselmis Viridis

The article presents the results of experimental evidence of the possibility of inexpensive mass cultivation of the Black Sea microalgae Tetraselmis viridis Rouch. The experiments were carried out in batch culture in pools under natural light and carbon supply by mixing and air aeration of the culture without additional supply of carbon dioxide. For 8 cm of the illuminated layer of the culture, the density of about 50 g (dry weight)/m2 or 88 billion cells/m2, with the productivity of 14.4 g (dry mass) m-2day-1 or 19 billion cells m-2day-1 has been reached.

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Influence of mussel Mytilus galloprovincialis exometabolites on R-phycoerythrin concentration in red alga Gelidium spinosum when grown in polyculture

Marine Biological Journal ◽

10.21072/mbj.2020.05.2.02 ◽

2020 ◽

Vol 5 (2) ◽

pp. 12-18

Author(s):

B. N. Belyaev ◽

N. M. Beregovaya

Keyword(s):

Black Sea ◽

Nutrient Medium ◽

Mytilus Galloprovincialis ◽

Sea Water ◽

Coastal Protection ◽

Mineral Salts ◽

Laboratory Installation ◽

Wet Weight ◽

Positive Effect ◽

Tetraselmis Viridis

To increase R-phycoerythrin concentration in red Black Sea alga Gelidium spinosum (S. G. Gmelin) P. C. Silva, 1996 (Rhodophyta), it was cultivated in laboratory conditions in polyculture microalga Tetraselmis viridis – mussel Mytilus galloprovincialis – Gelidium; the results of the study are presented. The positive effect of mussel exometabolites on R-phycoerythrin concentration in Gelidium in polyculture is described. The relevance of the work is determined by the value of R-phycoerythrin, which is used as a powerful antioxidant, as well as a marker in cytometry and microscopy. The aim of the study is to increase R-phycoerythrin concentration in Gelidium using the polyculture method. As a material, Gelidium from the fouling of rocks and coastal protection structures of Karantinnaya Bay (Sevastopol) was used; it was cultivated in a laboratory installation with eight working volumes, four of which contained mussels. Mussel decontamination, supplemented with mineral salts and biogens, was used as a nutrient medium for Gelidium. The combination of mussel exometabolites with previously developed nutrient medium, based on Black Sea water and enriched with nutrients and mineral salts, results in an increase in R-phycoerythrin concentration by more than 2 times, while the addition of exometabolites to pure filtered seawater increases it maximum by 35 %. Approximate ratios of polyculture elements in 1.5-L volumes, allowing to achieve the desired results in 2 weeks, are as follows: 2 g of Gelidium / 50–60 g of two-year-old mussels / 0.4–0.6 g of microalga wet weight.

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Changes in infectious titer of Black sea algal virus of the microalgae tetraselmis viridis under the influence of a constant magnetic field

Monitoring systems of environment ◽

10.33075/2220-5861-2020-4-35-39 ◽

2020 ◽

pp. 35-39

Author(s):

O.A. Stepanova ◽

◽

P.V. Gaisky ◽

S.A. Sholar ◽

◽

...

Keyword(s):

Magnetic Field ◽

Black Sea ◽

Magnetic Induction ◽

Constant Magnetic Field ◽

Algal Virus ◽

Infectious Titer ◽

Algal Viruses ◽

Order Of Magnitude ◽

Tetraselmis Viridis

In three experiments aimed at studying the effect of a constant magnetic field with a magnetic induction of 600 G and a duration of experiments of 24 and 36 h, it was found that with a low initial titer of the algal virus of the microalgae Tetraselmis viridis, its stay in a magnetic field for 24 h led to an increase in the infectious titer by one order of magnitude. However, when the virus with a higher infectious titer was in a magnetic field for 24 h, no changes in the titer were observed, and after 36 h, its decrease by one order of magnitude was recorded. The results obtained indicate the need for further research in this direction, with using a wider range of algal viruses.

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Response of marine microalgae Phaeodactylum tricornutum, Prorocentrum cordatum and Gyrodinium fissum to complex pollution of Sevastopol bays (Black Sea)

Ecologica Montenegrina ◽

10.37828/em.2021.48.13 ◽

2021 ◽

Vol 48 ◽

pp. 109-116

Author(s):

Liudmila Stelmakh ◽

Nela Kovrigina ◽

Tatiana Gorbunova

Keyword(s):

Black Sea ◽

Coastal Waters ◽

Phaeodactylum Tricornutum ◽

Laboratory Experiments ◽

Toxicity Assessment ◽

Marine Microalgae ◽

The Black Sea ◽

Organic Substances ◽

Taxonomic Affiliation ◽

Concentration Levels

For the degrees of toxicity assessment within the Black Sea surface coastal waters near Sevastopol, laboratory experiments were performed on three marine microalgae species culture’s growth in water sampled on the three stations of the region from May to December 2020. The study has indicated, that in the majority of cases, insignificant inhibiting influences of the waters to diatom Phaeodactylum tricornutum Bohlin and insignificant stimulated effect to dinoflagellates Prorocentrum сordatum (Ostenfeld) J.D.Dodge and Gyrodinium fissum (Levander) Kofoid & Swezy were observed. Rarely this effect was characterized as chronic or subacute. The concentration levels of both stable and unstable forms of organic substances in the studied waters were estimated. The results of the testing of the water to determine its contamination using microalgae and analysis of the organic substances concentration levels in them, allowed the conclusion that the studied area should be classified as episodically polluted. Justification for the necessity to use several microalgae species of different taxonomic affiliation when performing marine water biotesting is provided in this study.

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