scholarly journals CYTOGENETIC INDICATORS OF ACUTE RADIATION SICKNESS (THE CHORNOBYL EXPERIENCE)

2021 ◽  
Vol 26 ◽  
pp. 398-409
Author(s):  
E. Djomina ◽  
◽  
V. Talko ◽  
Keyword(s):  
Linear Regression ◽  
Multiple Linear Regression ◽  
Peripheral Blood ◽  
Chromosome Aberrations ◽  
Peripheral Blood Lymphocytes ◽  
Acute Radiation ◽  
Radiation Sickness ◽  
Acute Radiation Sickness ◽  
Blood Lymphocytes ◽  
Cytogenetic Data

The objective of the study was to improve the biological dosimetry approach among patients with acute radiation sickness of various degrees based on the analysis of radiation-induced chromosome aberrations in peripheral blood lymphocytes of the victims. Materials and methods. The study was based on primary cytogenetic data obtained in May 1986 within examination of the 30 clean-up workers («liquidators») having got stage I–III acute radiation sickness. Dose verification was performed using the cytogenetic dosimetry based on a culture of peripheral blood lymphocytes with metaphase analysis of chromosome aberrations. Results. A new method of evaluating the results of patients’ cytogenetic examination at the beginning of specific therapy has been developed. Procedure was performed using a model of multiple linear regression (complex of cytogenetic parameters) and provided a satisfactory diagnostic level (featuring a compliance with initially defined clinical and laboratory diagnoses). Overall frequency of the aberrant cells and radiation markers increased in higher disease stages. There was a trend of the frequency growth of chromatid-type aberrations with increasing of radiation burden. Adequacy of the proposed method based on the regression analysis of cytogenetic results was confirmed through the preservation of group differences in estimates of disease stage in subjects with verified diagnosis. Conclusion. Cytogenetic dosimetry in the scope of examination of persons exposed to ionizing radiation is an obligatory component of radiation sickness stage verification. The recommended method of cytogenetic data evaluation before and at the beginning of detoxification therapy provides a satisfactory level of diagnostics. Key words: acute radiation sickness, Chornobyl NPP accident, cytogenetic dosimetry, blood lymphocytes, chromosome aberrations, multiple linear regression model.

Chromosome aberrations in peripheral blood lymphocytes of high-risk HPV-infected women with HGSIL

2008 ◽  
Vol 49 (9) ◽  
pp. 688-694 ◽  
Author(s):  
Rosa E. Álvarez-Rosero ◽  
Jannet Rodríguez-Argote ◽  
Yexania Y. Arboleda-Moreno ◽  
Sulma L. Muñoz-Benítez ◽  
Carlos H. Sierra-Torres
Keyword(s):  
High Risk ◽  
Peripheral Blood ◽  
Chromosome Aberrations ◽  
Peripheral Blood Lymphocytes ◽  
Blood Lymphocytes ◽  
High Risk Hpv

scholarly journals Assessing the level of chromosome aberrations in peripheral blood lymphocytes in long-term resident children under conditions of high exposure to radon and its decay products

Mutagenesis ◽  
2015 ◽  
Vol 30 (5) ◽  
pp. 677-683 ◽  
Author(s):  
Vladimir G. Druzhinin ◽  
Maxim Yu. Sinitsky ◽  
Aleksey V. Larionov ◽  
Valentin P. Volobaev ◽  
Varvara I. Minina ◽  
...  
Keyword(s):  
Peripheral Blood ◽  
Chromosome Aberrations ◽  
Peripheral Blood Lymphocytes ◽  
High Exposure ◽  
Blood Lymphocytes ◽  
Decay Products

scholarly journals Chromosome Aberrations in Peripheral Blood Lymphocytes of Croatian Hospital Staff Occupationally Exposed to Low Levels of Ionising Radiation

2008 ◽  
Vol 59 (4) ◽  
pp. 251-259 ◽  
Author(s):  
Vilena Kašuba ◽  
Ružica Rozgaj ◽  
Anamarija Jazbec
Keyword(s):  
Peripheral Blood ◽  
Chromosome Aberrations ◽  
Direct Detection ◽  
Hospital Staff ◽  
Peripheral Blood Lymphocytes ◽  
Ionising Radiation ◽  
Control Group ◽  
Blood Lymphocytes ◽  
Occupationally Exposed ◽  
Low Levels

Chromosome Aberrations in Peripheral Blood Lymphocytes of Croatian Hospital Staff Occupationally Exposed to Low Levels of Ionising RadiationMedical staff is an occupational group exposed to different agents suspected to induce genetic damage. Among them ionising radiation is the most studied. Cytogenetic analysis of human chromosomes in peripheral lymphocytes allows direct detection of mutation in somatic cells. This study investigated the cytogenetic effects of low-level ionising x-radiation in 48-hour peripheral blood lymphocyte cultures sampled from 765 hospital staff occupationally exposed to several agents known or suspected to induce chromosome damage and compared them with 200 control subjects. The exposed subjects were divided in eight (8) groups according to their specialities and job titles. The exposed groups manifested an increase in all types of chromosome aberrations. Acentric fragments were the most frequent chromosome-type aberration. Dicentric chromosomes were statistically significant only in urologists/gynaecologists. Age and smoking significantly influenced the incidence of dicentrics in the exposed groups. The frequency of ring chromosomes was low in all exposed groups (range: 0-2), and none were found in the control group. These findings indicate the importance of periodic medical checkups of hospital staff occupationally exposed to low doses of ionising radiation. The purpose is to create an individual cytogenetic register, where changes could evidence individual risks.

scholarly journals Assessment of Potential Clastogenic Effect of the Insecticide Mospilan® 20SP in Human Peripheral Blood Lymphocytes After in Vitro Exposure

2019 ◽  
Vol 63 (2) ◽  
pp. 21-24
Author(s):  
K. Ničová ◽  
V. Schwarzbacherová ◽  
M. Galdíková ◽  
B. Holečková
Keyword(s):  
Peripheral Blood ◽  
Chromosome Aberrations ◽  
Human Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Blood Lymphocytes ◽  
Human Peripheral Blood Lymphocytes ◽  
Neonicotinoid Insecticide ◽  
Clastogenic Effect ◽  
Chromosome Aberration Test

Abstract Acetamiprid, that is known as the commercial formulation Mospilan® 20SP is the part of the neonicotinoid insecticide group and is widely used against various pests. In our study we assessed the potential clastogenic effects of Mospilan® in human peripheral blood lymphocytes in vitro using a chromosome aberration test. The lymphocytes were treated with acetamiprid in the concentration range of 5, 10, 25 and 50 µg.ml−1 for 24 and 48 h. After 24 h exposure, the insecticide induced statistically significant higher levels of chromosome aberrations from the concentration of 10 µg.ml−1 (P < 0.05 and P < 0.001) and a significant decrease in mitotic index (MI) at the concentrations of 25 and 50 µg.ml−1 (P < 0.05 and P < 0.01), respectively. After a 48 h exposure, we found a dose dependent increase in the percentage of chromosome aberrations at all concentrations (P < 0.05; P < 0.01 and P < 0.001) and a decrease in MI at concentrations of 25 and 50 µg.ml−1 (P < 0.05 and P < 0.01). Our results indicated that neonicotinoid insecticide formulations containing acetamiprid may have potential cytotoxic and genotoxic effects.

Chromosome aberrations in peripheral blood lymphocytes from control individuals

1995 ◽  
Vol 346 (4) ◽  
pp. 187-193 ◽  
Author(s):  
Vilena Kašuba ◽  
Karmela Šentija ◽  
Vera Garaj-Vrhova ◽  
Aleksandra Fučić
Keyword(s):  
Peripheral Blood ◽  
Chromosome Aberrations ◽  
Peripheral Blood Lymphocytes ◽  
Blood Lymphocytes
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