scholarly journals mRNA extraction, cDNA synthesis and tillering specific gene isolation from BLB resistant Binashail rice

2015 ◽  
Vol 1 (2) ◽  
pp. 265-270
Author(s):  
Papia Jahan ◽  
Ahmed Hossain ◽  
KM Nasiruddin ◽  
Sabina Yasmin ◽  
Fahmida Khatun ◽  
...  

Bacterial Leaf Blight (BLB) caused by Xanthomonas oryzaepv. oryzae (Xoo) bacteria, is a major biotic stress in the irrigated rice belts. Genetic modification is the most effective and economical control for bacterial blight disease. For high yielding, branching capacity is most important. Molecular survey was conducted to identify the presence of tillering specific gene in BLB resistant Binashail rice. Reproducible means of Binashail rice at 21 days was designed based on the timing of full expression of the leaf. PCR with primers specific for tillering specific gene was used in the study. The cDNA of Binashail rice synthesized by an improved RT-PCR technique, derived from the total mRNAs extracted from the plant leaf. Gel documentation showed that the size of the synthesized cDNA was 250bp and tillering specific gene was 201bp in length long. The identification of tillering specific gene in Binashal rice germplasm will help in accelerating the elite breeding program in future.Asian J. Med. Biol. Res. June 2015, 1(2): 265-270

2018 ◽  
Vol 48 (2) ◽  
pp. 143-158 ◽  
Author(s):  
Liyuan Zhang ◽  
Yiqun Hu ◽  
Ping Li ◽  
Xiaobing Wang ◽  
Hansong Dong

2017 ◽  
Vol 165 (11-12) ◽  
pp. 727-736 ◽  
Author(s):  
Konduvally Annegowda Lalithya ◽  
Girigowda Manjunatha ◽  
Besagarahally Raju ◽  
Mukund S. Kulkarni ◽  
Veeresh Lokesh

2020 ◽  
Vol 42 (2) ◽  
Author(s):  
Menson R. Kwanwah ◽  
Tanawat Wongsa ◽  
Tidarat Monkham ◽  
Sompong Chankaew ◽  
Shanerin Falab ◽  
...  

1992 ◽  
Vol 236 (1) ◽  
pp. 113-120 ◽  
Author(s):  
Pamela C. Ronald ◽  
Beng Albano ◽  
Rodante Tabien ◽  
Lleva Abenes ◽  
Kung-sheng Wu ◽  
...  

2018 ◽  
Vol 51 (1) ◽  
pp. 45-50
Author(s):  
P.B. Sandipan ◽  
R.K. Patel ◽  
G.O. Faldu ◽  
D.M. Patel ◽  
B.G. Solanki

Abstract Cotton is a soft, fluffy staple fiber that grows in a boll, around the seeds of the cotton plants of the genus Gossypium in the family Malvaceae. Bacterial blight of cotton, also know as angular leaf spot, boll rot, and black leg, is a potentially destructive bacterial disease of cotton production. The disease caused by Xanthomonas campestris pv. malvacearum (Smith) Dye (synonyms Xanthomonas malvacearum (E.F. Sm) Dowson) is one of the most important and serious disease in cotton crop. Main objective of present research is to study the progress of the bacterial blight disease of cotton (BLB), caused by Xanthomonas campestris pv. malvacearum (Smith) Dye, with relation to the environmental parameters. This is a common disease affecting the growth, development and yield of cotton. A field trial was conducted to determine the influence of environmental factors, viz. rainfall periods, temperature, sun shine hours and humidity on the development of disease. Bacterial blight disease was recorded with its appearance and subsequently at weekly interval till it prevailed on G. Cot. Hy.12 (Non Bt). The incidence of bacterial blight disease (BLB) was noticed during 28 to 49th standard week with the maximum disease intensity in third week of September (23.5% PDI). None of the abiotic factors had significant influence on bacterial blight disease progress and development.


2012 ◽  
Vol 80 ◽  
pp. 1-9 ◽  
Author(s):  
Selvamathiazhagan Nisha ◽  
Kannan Revathi ◽  
Rajamanickam Chandrasekaran ◽  
Suyambulingam Arunachalam Kirubakaran ◽  
Subbiah Sathish-Narayanan ◽  
...  

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