Assessment of self-incompatibility in some improved and traditional cultivars of oleiferous (Brassica rapa L.)

2007 ◽  
Vol 32 (2) ◽  
Author(s):  
MA Zilani Chowdhury ◽  
MA Khaleque Mian ◽  
M Ali Akbar
Keyword(s):  
Brassica Rapa ◽  
Self Incompatibility ◽  
Traditional Cultivars

scholarly journals Molecular Characterization of S Locus Genes, SLG and SRK, in a Pollen-Recessive Self-Incompatibility Haplotype of Brassica rapa L

Genetics ◽  
1998 ◽  
Vol 149 (3) ◽  
pp. 1587-1597 ◽  
Author(s):  
Katsunori Hatakeyama ◽  
Takeshi Takasaki ◽  
Masao Watanabe ◽  
Kokichi Hinata
Keyword(s):  
Brassica Rapa ◽  
Genomic Dna ◽  
Transmembrane Domain ◽  
Brassica Species ◽  
Self Incompatibility ◽  
S Locus ◽  
Intron Sequence ◽  
High Degree ◽  
Degree Of Similarity

Abstract In Brassica species that exhibit self-incompatibility, two genes, SLG and SRK, at the S locus are involved in the recognition reaction with self and non-self pollen. From a pollen-recessive S29 haplotype of Brassica rapa, both cDNA and genomic DNA clones for these two genes were isolated and characterized. The nucleotide sequence for the S domain of SRK29 showed a high degree of similarity with that of SLG29, and they belong to Class II type. RNA gel blot analysis showed that the transcript of SLG29 consisted of the first and second exons, and no other transcript containing any part of the intron sequence was detected. Because no transmembrane domain was encoded by the second exon of SLG29, SLG29 was designated a secreted type glycoprotein. SLGs of two other pollen-recessive haplotypes, S40 and S44, of B. rapa also had a similar structure to that of SLG29. Previously, SLG2 from a pollen-recessive haplotype, S2, of Brassica oleracea was found to produce two different transcripts, one for the secreted type glycoprotein and the other for a putative membrane-anchored form of SLG. Therefore, the nature of these SLGs from pollen-recessive haplotypes of B. rapa is different from that of SLG2 of B. oleracea.

scholarly journals Genetic and tissue-specific RNA-sequencing analysis of self-compatible mutant TSC28 in Brassica rapa L. toward identification of a novel self-incompatibility factor

2019 ◽  
Vol 94 (4) ◽  
pp. 167-176
Author(s):  
Masaaki Osaka ◽  
Moe Nabemoto ◽  
Shunsuke Maeda ◽  
Satomi Sakazono ◽  
Hiromi Masuko-Suzuki ◽  
...  
Keyword(s):  
Rna Sequencing ◽  
Brassica Rapa ◽  
Sequencing Analysis ◽  
Self Incompatibility ◽  
Tissue Specific ◽  
Incompatibility Factor

scholarly journals The initial analysis of the ability for the generative propagation and the level of self-incompatibility of inbred lines of chinese cabbage (Brassica rapa ssp. Pekinensis)

2013 ◽  
Vol 21 (2) ◽  
pp. 109-113
Author(s):  
Piotr Kamiński
Keyword(s):  
Seed Development ◽  
Brassica Rapa ◽  
Inbreeding Depression ◽  
Chinese Cabbage ◽  
Weather Conditions ◽  
Inbred Lines ◽  
The Other ◽  
Self Incompatibility ◽  
Open Flower ◽  
Flower Stage

ABSTRACT In the years of 2011 and 2013, seventeen inbred lines of Chinese cabbage were evaluated according to the ability for the generative propagation and the expression of self-incompatibility. Most of the lines pollinated in the greenhouse at the open flower stage were characterized by an intermediate level of selfincompatibility and formed less than one seed/silique; the other lines were highly self-incompatible or selfcompatible. The lines pollinated at the green bud stage were highly diversified according to the ability for seed development in both years of propagation; however, most of the lines formed sufficient amount of seeds for use as parental components. The average effectiveness of generative propagation after pollination at the open flower and green bud stages was higher in 2011 than in 2013, probably due to different weather conditions in these years influencing the seed development or inbreeding depression.

Mapping of quantitative trait loci for high level of self-incompatibility in Brassica rapa L.

Genome ◽  
2010 ◽  
Vol 53 (4) ◽  
pp. 257-265 ◽  
Author(s):  
Katsunori Hatakeyama ◽  
Atsushi Horisaki ◽  
Satoshi Niikura ◽  
Yoshihiro Narusaka ◽  
Hiroshi Abe ◽  
...  
Keyword(s):  
Quantitative Trait Loci ◽  
Brassica Rapa ◽  
Inbred Line ◽  
Quantitative Trait ◽  
Self Incompatibility ◽  
Insect Pollination ◽  
Trait Loci ◽  
Variation Explained ◽  
Brassica Vegetable ◽  
High Level

The level of self-incompatibility (SI) is important to the purity of F1 seeds produced using the SI system of Brassica vegetables. To analyze the genetic basis of the level of SI, we generated an F2 population derived from a cross between a turnip inbred line showing a high level of SI and a Chinese cabbage inbred line showing a low level, and evaluated the level of SI under insect pollination in two years. We constructed a detailed linkage map of Brassica rapa from the F2 progeny, consisting of SSR, SNP, indel, and CAPS loci segregating into 10 linkage groups covering approximately 700 cM. Five quantitative trait loci (QTL) for high-level SI were identified. The phenotypic variation explained by the QTL ranged between 7.2% and 23.8%. Two QTL were detected in both years. Mapping of SI-related genes revealed that these QTL were co-localized with SLG on R07 and MLPK on R03. This is the first report of QTL for high-level SI evaluated under insect pollination in a Brassica vegetable. Our results could be useful for the marker-assisted selection of parental lines with a stable SI.

scholarly journals 048 Effects of Inbreeding in a “Wisconsin Fast Plants” Brassica rapa Population

HortScience ◽  
2000 ◽  
Vol 35 (3) ◽  
pp. 396E-396
Author(s):  
Federico L. Iniguez Luy ◽  
James Nienhuis
Keyword(s):  
Brassica Rapa ◽  
Genetic Variance ◽  
Inbred Lines ◽  
Seed Color ◽  
Rapid Cycling ◽  
Self Incompatibility ◽  
Anthocyanin Pigmentation ◽  
Sib Mating ◽  
Two Generations ◽  
Mutant Phenotypes

Wisconsin Fast Plants (WFP) are small, rapid-cycling Brassica rapa populations that were developed by Paul Williams in the Dept. of Plant Pathology at the Univ. of Wisconsin, to facilitate classroom demonstration of biological principals. WFP exist as heterogenous populations, which have been selected for expression of different mutant phenotypes. Because of self-incompatibility mechanisms, it has been difficult to develop inbred lines of WFP via self-feritilization. Our objective was to inbreed a WFP population through full-sib mating. Genetic diversity was calculated for eight individuals from each of eight different WFF population, using 69 polymorphic RAPD (molecular marker) bands. The eight different WPF populatons were randomly mated (via chain crossing) for two generations. Six cycles of full-sib mating were initiated on 130 random families. After six cycles of full-sib mating, 79 families remain. The loss of families, during the process of inbreeding, may have been due to selection or drift. However, the expectation is that genetic variance will increase. The 79 inbred families express an array of different WFP phenotypes, e.g., anthocyanin pigmentation, yellow cotyledon, plant height, and seed color at different combinations in different inbred lines.

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