Molecular assessment of maize inbred lines (Zea mays L.) Using microsatellite markers

2018 ◽  
Vol 43 (4) ◽  
pp. 533-542
Author(s):  
Maniruzzaman ◽  
MG Azam ◽  
S Islam ◽  
MG Hossain ◽  
MM Rohman
Keyword(s):  
Genetic Diversity ◽  
Plant Breeding ◽  
Genetic Relatedness ◽  
Agricultural Research ◽  
Inbred Lines ◽  
The Other ◽  
Diversity Analysis ◽  
Genetic Diversity Analysis ◽  
Maize Inbred Lines ◽  
Maize Genotypes

Genetic diversity analysis and germplasm characterization are essential steps in plant breeding and molecular markers are proved tool to accomplish. The present study was undertaken at the Molecular Breeding Lab of Plant Breeding Division, Bangladesh Agricultural Research Institute (BARI) to determine the genetic relatedness and molecular characterization of 15 maize inbred lines of BARI. In present study, genetic diversity analysis was performed by using 10 SSR primers to evaluate the polymorphisms, among them six primers showed distinct polymorphism between the maize inbred lines. The maize genotypes E81, E144, E08, E167, E102, E142 and E121 were found more diverged (0.9003) compared to other inbred lines. On the other hand, the lowest genetic distance values (0.1501) were found between the genotype E140 and genotype E80 followed by genotype E126 and genotype E140; genotype E140 and genotype E65; genotype E65 and genotype E80 values were identical (0.4502). The genotypes viz. E81, E144, E08, E167, E102, E142 and E121 were found far away from centroid of the cluster and rest of the genotypes were placed around the centroid. The Principal Coordinate Analysis (PCO) helped to visualize four major clusters and showed that seven maize inbred lines (E81, E58, E08, E167, E102, E142 and E121) were far away from the other genotypes. In conclusion, SSR markers enabled discrimination among accessions and provided valuable information for future use in improvement of these genomic resources.Bangladesh J. Agril. Res. 43(4): 533-542, December 2018

Genetic diversity analysis of 119 Canadian maize inbred lines based on pedigree and simple sequence repeat markers

2011 ◽  
Vol 91 (4) ◽  
pp. 651-661 ◽  
Author(s):  
L. M. Reid ◽  
K. Xiang ◽  
X. Zhu ◽  
B. R. Baum ◽  
S. J. Molnar
Keyword(s):  
Genetic Diversity ◽  
Simple Sequence Repeat ◽  
Inbred Lines ◽  
Diversity Analysis ◽  
Sequence Repeat ◽  
Heterotic Groups ◽  
Simple Sequence Repeat Markers ◽  
Genetic Diversity Analysis ◽  
Maize Inbred Lines ◽  
Simple Sequence

Reid, L. M., Xiang, K., Zhu, X., Baum, B. R. and Molnar, S. J. 2011. Genetic diversity analysis of 119 Canadian maize inbred lines based on pedigree and simple sequence repeat markers. Can. J. Plant Sci. 91: 651–661. Since the early 1920s Agriculture and Agri-Food Canada (AAFC) has been developing maize varieties and inbred lines adapted to the early maize growing regions. These inbreds represent a large group of genetically diverse genotypes; however, many of the inbreds are of unknown heterotic backgrounds. The purpose of this study was to classify 119 elite maize inbred lines released from AAFC into heterotic groups using both pedigree data and simple sequence repeat (SSR) markers and also to explore the consistency among different classification analyses. Pedigree analysis placed the inbred lines into eight groups, six of which corresponded to known major heterotic groups representing Iowa Stiff Stalk Synthetic (BSSS), European flint, Lancaster, Minnesota 13, Early Butler, and Iodent; the two remaining groups consisted of germplasm derived mostly from Pioneer 3990 or Pioneer 3994 sources. Simple sequence repeat analysis of 105 loci resulted in a clustering of the inbreds into 10 groups. In comparison with the grouping based on pedigree, the SSR clustering groups had some discrepancies and groups of genetically similar germplasm, based on pedigree, could not always be confirmed with molecular markers. The results of this study will allow researchers and maize breeders to make more informed decisions on the use of these inbreds in breeding programs.

scholarly journals Genetic Diversity Analysis of Maize Inbred Lines from SCDA Turda - Romania Revealed by RAPD Molecular Markers

2011 ◽  
Vol 68 (1) ◽  
Author(s):  
Rodica POP ◽  
Ioan HAS ◽  
Iulia Francesca POPESCU ◽  
Monica HARTA ◽  
Doru PAMFIL
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Phylogenetic Relationships ◽  
Inbred Lines ◽  
Genetic Distances ◽  
Diversity Analysis ◽  
Genetic Diversity Analysis ◽  
Upgma Dendrogram ◽  
Maize Inbred Lines ◽  
Polymorphic Bands

Knowledge of genetic diversity and relationships among maize inbred lines is indispensable to a breeding program. Our objective was to investigate the level of genetic diversity among maize inbred lines. Eighty-three maize inbred lines obtained from SCDA Turda were genotyped using 20 decamer primers. These primers generated, among the studied genotypes, a number of polymorphic bands comprised between 17 bands (OPA 03) and 7 bands (OPAB 11). The highest numbers of polymorphic bands were obtained with primer OPA 03, respectively 17 bands, followed by OPA 01, OPB 08 (16 polymorphic bands) and OPX 03 and OPAL 20 (13 polymorphic bands). Genetic distances were established using Nei Li/Dice coefficent and an UPGMA dendrogram was constructed with FreeTree software. The built dendrogram shows phylogenetic relationships between the analysed biological material.

scholarly journals Applications of molecular markers in Genetic Diversity Studies of maize

2020 ◽  
Vol 37 (1) ◽  
pp. 101-108
Author(s):  
Degife Asefa Zebire
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Inbred Lines ◽  
Hybrid Breeding ◽  
Diversity Analysis ◽  
Crop Species ◽  
Heterotic Groups ◽  
Genetic Diversity Analysis ◽  
Diversity Studies ◽  
Genetic Diversity Study

Molecular markers are efficient for exploiting variations in genotypes as they are not influenced by environmental factors and also speed up breeding programs. They are used to detect large numbers of distinct divergence between genotypes at the DNA level. Genetic diversity study helps to estimate the relationship between inbred lines to make the best hybrid combinations. Lines which are clustered in different heterotic groups are considered as the best hybrid combinations to carry out further breeding activities. Molecular markers are used to meet a number of objectives, including genetic diversity analysis and prediction of hybrid performances in divergent crop species. Agro-morphological and molecular markers have been utilized to study genetic diversity so far. In maize, the uses of molecular markers are important for the evaluation of genetic diversity of inbred lines and in clustering them into heterotic groups. These markers determine genetic similarity of the lines and are used to assess the genetic diversity of maize. Molecular markers have proven valuable for genetic diversity analysis of many crop species and genetically diverse lines are important to improve hybrid breeding. Keyword: Molecular marker; Genetic diversity; Genetic variation, Diversity Array technology; cluster analysis

scholarly journals Development of Pineapple Microsatellite Markers and Germplasm Genetic Diversity Analysis

2013 ◽  
Vol 2013 ◽  
pp. 1-11 ◽  
Author(s):  
Suping Feng ◽  
Helin Tong ◽  
You Chen ◽  
Jingyi Wang ◽  
Yeyuan Chen ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Genomic Library ◽  
Trinucleotide Repeats ◽  
The Other ◽  
Diversity Analysis ◽  
Dominant Type ◽  
Genetic Diversity Analysis ◽  
Ssr Loci ◽  
Ssr Development

Two methods were used to develop pineapple microsatellite markers. Genomic library-based SSR development: using selectively amplified microsatellite assay, 86 sequences were generated from pineapple genomic library. 91 (96.8%) of the 94 Simple Sequence Repeat (SSR) loci were dinucleotide repeats (39 AC/GT repeats and 52 GA/TC repeats, accounting for 42.9% and 57.1%, resp.), and the other three were mononucleotide repeats. Thirty-six pairs of SSR primers were designed; 24 of them generated clear bands of expected sizes, and 13 of them showed polymorphism. EST-based SSR development: 5659 pineapple EST sequences obtained from NCBI were analyzed; among 1397 nonredundant EST sequences, 843 were found containing 1110 SSR loci (217 of them contained more than one SSR locus). Frequency of SSRs in pineapple EST sequences is 1SSR/3.73 kb, and 44 types were found. Mononucleotide, dinucleotide, and trinucleotide repeats dominate, accounting for 95.6% in total. AG/CT and AGC/GCT were the dominant type of dinucleotide and trinucleotide repeats, accounting for 83.5% and 24.1%, respectively. Thirty pairs of primers were designed for each of randomly selected 30 sequences; 26 of them generated clear and reproducible bands, and 22 of them showed polymorphism. Eighteen pairs of primers obtained by the one or the other of the two methods above that showed polymorphism were selected to carry out germplasm genetic diversity analysis for 48 breeds of pineapple; similarity coefficients of these breeds were between 0.59 and 1.00, and they can be divided into four groups accordingly. Amplification products of five SSR markers were extracted and sequenced, corresponding repeat loci were found and locus mutations are mainly in copy number of repeats and base mutations in the flanking region.

Genetic diversity analysis of sweet potato [Ipomoea batatas (L.) Lam.] germplasms through RAPD and ISSR markers

2021 ◽  
Vol 50 (1) ◽  
pp. 119-129
Author(s):  
PN Narasimha Murthy ◽  
NB Patel ◽  
Chintan Kapadia ◽  
KD Desai ◽  
G Koteswara Rao
Keyword(s):  
Genetic Diversity ◽  
Sweet Potato ◽  
Ipomoea Batatas ◽  
Genetic Relatedness ◽  
Issr Markers ◽  
Diversity Analysis ◽  
Genetic Diversity Analysis ◽  
Polymorphic Loci ◽  
Improvement Programme ◽  
Rapd And Issr

Sweet potato [Ipomoea batatas (L.) Lam.] germplasms are unique as they are heterozygous, vegitatively as well as sexually propagated and polyploid in nature. Forty-four germplasms from local farmer’s field and from various centres of India were determined using 15 RAPD and 15 ISSR markers to evaluate their genetic relatedness. Average polymorphisms for RAPD and ISSR markers were found to be 86.72 and 81.64%, respectively. The OPM series primer produced 145 total bands with 138 polymorphic loci and ISSR markers gave 116 total bands with 101 polymorphic loci. The clusters were made using the UPGMA method using both the RAPD and ISSR data and group diverse yet similar germplasm in two different clusters. This gives ample information to use them in further improvement programme.

scholarly journals (235) Genetic Diversity Analysis of Five Passiflora Genotypes

HortScience ◽  
2005 ◽  
Vol 40 (4) ◽  
pp. 1002A-1002
Author(s):  
Eric Stafne ◽  
Jon Lindstrom ◽  
John Clark
Keyword(s):  
Genetic Diversity ◽  
North American ◽  
Genetic Similarity ◽  
The Other ◽  
Diversity Analysis ◽  
Breeding Programs ◽  
Genetic Diversity Analysis ◽  
Native North American ◽  
Cold Hardy ◽  
High Degree

Passiflora is an important ornamental genus, mainly within tropical zones. However, two cold-hardy, North American Passiflora species exist. Previous work has been done to incorporate these species into breeding programs with some success. The intent of this study was to evaluate the extent of genetic diversity among five different Passiflora genotypes, including the two native North American species, P. incarnata L. and P. lutea L. Results indicate low genetic similarity among all genotypes with none at 50% or greater. P. incarnata and the ornamental cultivar `Lady Margaret' displayed the highest relationship at 49%. P. incarnata averaged 35.5% similarity with the other genotypes and P. lutea was 29.5%. Average overall similarity among all genotypes was 31.1%. These and other results show that the Passiflora genus has a high degree of genetic variation and breeding efforts could expand interest within North America.

MOLECULAR CHARACTERIZATION AND DIVERSITY ANALYSIS OF SELECTED MAIZE INBRED LINES USING SINGLE NUCLEOTIDE POLYMORPHISM MARKERS

2020 ◽  
Author(s):  
Mwaikonyole Zawadi ◽  
Julia Sibiya ◽  
Kingstone Mashingaidze ◽  
Assefa B Amelework ◽  
Aleck Kondwakwenda ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Single Nucleotide Polymorphism ◽  
Agricultural Research ◽  
Gene Diversity ◽  
Polymorphic Information Content ◽  
Inbred Lines ◽  
Nucleotide Polymorphism ◽  
Heterotic Groups ◽  
Single Nucleotide ◽  
Maize Inbred Lines

The level of genetic diversity among the available breeding materials determines the potential success of a breeding program. In this study, 92 maize inbred lines were genotyped with 3047 single nucleotide polymorphism (SNP) markers using a Kompetitive Allele-Specific Polymerase chain reaction (KASPTM) genotyping protocol. The objectives were to determine the level and pattern of genetic diversity and define potential heterotic groups of maize inbred lines developed by the Agricultural Research Council maize program of South Africa. More than 91% of the SNPs used were polymorphic with mean polymorphic information content (PIC) of 0.36. Gene diversity ranged from 0.35 to 0.37, with a mean of 0.36. Cluster analysis revealed the presence of three distinct subpopulations. Analysis of molecular variance revealed low but highly significant (p<0.0001) variations among populations, high within and among individual variations. Variation among individuals contributed 83% of the total variation, whereas variation within individuals and among populations contributed 14% and 3%, respectively. Low mean population differentiation observed in this study suggested that the inbred lines might be developed from parental genotypes with similar genetic backgrounds. The mean percentage of genetic purity among the inbred lines was 4.8, with more than 79% of the inbred lines exhibiting less than 5% heterozygosity, indicating the inbred lines are fixed. Genotypes in different clusters may be earmarked as belonging to distinct heterotic groups, and their crosses may result in better heterosis. Thus, the findings of this study set the basis for earmarking heterotic groups and parental selection.

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