scholarly journals Genetic Diversity Analysis of Maize Inbred Lines from SCDA Turda - Romania Revealed by RAPD Molecular Markers

2011 ◽  
Vol 68 (1) ◽  
Author(s):  
Rodica POP ◽  
Ioan HAS ◽  
Iulia Francesca POPESCU ◽  
Monica HARTA ◽  
Doru PAMFIL
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Phylogenetic Relationships ◽  
Inbred Lines ◽  
Genetic Distances ◽  
Diversity Analysis ◽  
Genetic Diversity Analysis ◽  
Upgma Dendrogram ◽  
Maize Inbred Lines ◽  
Polymorphic Bands

Knowledge of genetic diversity and relationships among maize inbred lines is indispensable to a breeding program. Our objective was to investigate the level of genetic diversity among maize inbred lines. Eighty-three maize inbred lines obtained from SCDA Turda were genotyped using 20 decamer primers. These primers generated, among the studied genotypes, a number of polymorphic bands comprised between 17 bands (OPA 03) and 7 bands (OPAB 11). The highest numbers of polymorphic bands were obtained with primer OPA 03, respectively 17 bands, followed by OPA 01, OPB 08 (16 polymorphic bands) and OPX 03 and OPAL 20 (13 polymorphic bands). Genetic distances were established using Nei Li/Dice coefficent and an UPGMA dendrogram was constructed with FreeTree software. The built dendrogram shows phylogenetic relationships between the analysed biological material.

Molecular assessment of maize inbred lines (Zea mays L.) Using microsatellite markers

2018 ◽  
Vol 43 (4) ◽  
pp. 533-542
Author(s):  
Maniruzzaman ◽  
MG Azam ◽  
S Islam ◽  
MG Hossain ◽  
MM Rohman
Keyword(s):  
Genetic Diversity ◽  
Plant Breeding ◽  
Genetic Relatedness ◽  
Agricultural Research ◽  
Inbred Lines ◽  
The Other ◽  
Diversity Analysis ◽  
Genetic Diversity Analysis ◽  
Maize Inbred Lines ◽  
Maize Genotypes

Genetic diversity analysis and germplasm characterization are essential steps in plant breeding and molecular markers are proved tool to accomplish. The present study was undertaken at the Molecular Breeding Lab of Plant Breeding Division, Bangladesh Agricultural Research Institute (BARI) to determine the genetic relatedness and molecular characterization of 15 maize inbred lines of BARI. In present study, genetic diversity analysis was performed by using 10 SSR primers to evaluate the polymorphisms, among them six primers showed distinct polymorphism between the maize inbred lines. The maize genotypes E81, E144, E08, E167, E102, E142 and E121 were found more diverged (0.9003) compared to other inbred lines. On the other hand, the lowest genetic distance values (0.1501) were found between the genotype E140 and genotype E80 followed by genotype E126 and genotype E140; genotype E140 and genotype E65; genotype E65 and genotype E80 values were identical (0.4502). The genotypes viz. E81, E144, E08, E167, E102, E142 and E121 were found far away from centroid of the cluster and rest of the genotypes were placed around the centroid. The Principal Coordinate Analysis (PCO) helped to visualize four major clusters and showed that seven maize inbred lines (E81, E58, E08, E167, E102, E142 and E121) were far away from the other genotypes. In conclusion, SSR markers enabled discrimination among accessions and provided valuable information for future use in improvement of these genomic resources.Bangladesh J. Agril. Res. 43(4): 533-542, December 2018

scholarly journals Applications of molecular markers in Genetic Diversity Studies of maize

2020 ◽  
Vol 37 (1) ◽  
pp. 101-108
Author(s):  
Degife Asefa Zebire
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Inbred Lines ◽  
Hybrid Breeding ◽  
Diversity Analysis ◽  
Crop Species ◽  
Heterotic Groups ◽  
Genetic Diversity Analysis ◽  
Diversity Studies ◽  
Genetic Diversity Study

Molecular markers are efficient for exploiting variations in genotypes as they are not influenced by environmental factors and also speed up breeding programs. They are used to detect large numbers of distinct divergence between genotypes at the DNA level. Genetic diversity study helps to estimate the relationship between inbred lines to make the best hybrid combinations. Lines which are clustered in different heterotic groups are considered as the best hybrid combinations to carry out further breeding activities. Molecular markers are used to meet a number of objectives, including genetic diversity analysis and prediction of hybrid performances in divergent crop species. Agro-morphological and molecular markers have been utilized to study genetic diversity so far. In maize, the uses of molecular markers are important for the evaluation of genetic diversity of inbred lines and in clustering them into heterotic groups. These markers determine genetic similarity of the lines and are used to assess the genetic diversity of maize. Molecular markers have proven valuable for genetic diversity analysis of many crop species and genetically diverse lines are important to improve hybrid breeding. Keyword: Molecular marker; Genetic diversity; Genetic variation, Diversity Array technology; cluster analysis

Genetic diversity analysis of 119 Canadian maize inbred lines based on pedigree and simple sequence repeat markers

2011 ◽  
Vol 91 (4) ◽  
pp. 651-661 ◽  
Author(s):  
L. M. Reid ◽  
K. Xiang ◽  
X. Zhu ◽  
B. R. Baum ◽  
S. J. Molnar
Keyword(s):  
Genetic Diversity ◽  
Simple Sequence Repeat ◽  
Inbred Lines ◽  
Diversity Analysis ◽  
Sequence Repeat ◽  
Heterotic Groups ◽  
Simple Sequence Repeat Markers ◽  
Genetic Diversity Analysis ◽  
Maize Inbred Lines ◽  
Simple Sequence

Reid, L. M., Xiang, K., Zhu, X., Baum, B. R. and Molnar, S. J. 2011. Genetic diversity analysis of 119 Canadian maize inbred lines based on pedigree and simple sequence repeat markers. Can. J. Plant Sci. 91: 651–661. Since the early 1920s Agriculture and Agri-Food Canada (AAFC) has been developing maize varieties and inbred lines adapted to the early maize growing regions. These inbreds represent a large group of genetically diverse genotypes; however, many of the inbreds are of unknown heterotic backgrounds. The purpose of this study was to classify 119 elite maize inbred lines released from AAFC into heterotic groups using both pedigree data and simple sequence repeat (SSR) markers and also to explore the consistency among different classification analyses. Pedigree analysis placed the inbred lines into eight groups, six of which corresponded to known major heterotic groups representing Iowa Stiff Stalk Synthetic (BSSS), European flint, Lancaster, Minnesota 13, Early Butler, and Iodent; the two remaining groups consisted of germplasm derived mostly from Pioneer 3990 or Pioneer 3994 sources. Simple sequence repeat analysis of 105 loci resulted in a clustering of the inbreds into 10 groups. In comparison with the grouping based on pedigree, the SSR clustering groups had some discrepancies and groups of genetically similar germplasm, based on pedigree, could not always be confirmed with molecular markers. The results of this study will allow researchers and maize breeders to make more informed decisions on the use of these inbreds in breeding programs.

Molecular markers and antioxidant activity in berry crops: Genetic diversity analysis

2012 ◽  
Vol 92 (6) ◽  
pp. 1121-1133 ◽  
Author(s):  
S. C. Debnath ◽  
Y. L. Siow ◽  
J. Petkau ◽  
D. An ◽  
N. V. Bykova
Keyword(s):  
Genetic Diversity ◽  
Antioxidant Activity ◽  
Molecular Markers ◽  
Molecular Techniques ◽  
Diversity Analysis ◽  
Full Potential ◽  
Genetic Diversity Analysis ◽  
Wide Range ◽  
Berry Crops ◽  
Improvement Programs

Debnath, S. C., Siow, Y. L., Petkau, J., An, D. and Bykova, N. V. 2012. Molecular markers and antioxidant activity in berry crops: Genetic diversity analysis. Can. J. Plant Sci. 92: 1121–1133. An improved understanding of important roles of dietary fruits in maintaining human health has led to a dramatic increase of global berry crop production. Berry fruits contain relatively high levels of vitamin C, cellulose and pectin, and produce anthocyanins, which have important therapeutic values, including antitumor, antiulcer, antioxidant and anti-inflammatory activities. There is a need to develop reliable methods to identify berry germplasm and assess genetic diversity/relatedness for dietary properties in berry genotypes for practical breeding purposes through genotype selection in a breeding program for cultivar development, and proprietary-rights protection. The introduction of molecular biology techniques, such as DNA-based markers, allows direct comparison of different genetic materials independent of environmental influences. Significant progress has been made in diversity analysis of wild cranberry, lowbush blueberry, lingonberry and cloudberry germplasm, and in strawberry and raspberry cultivars and advanced breeding lines developed in Canada. Inter simple sequence repeat (ISSR) markers detected an adequate degree of polymorphism to differentiate among berry genotypes, making this technology valuable for cultivar identification and for the more efficient choice of parents in the current berry improvement programs. Although multiple factors affect antioxidant activity, a wide range of genetic diversity has been reported in wild and cultivated berry crops. Diversity analysis based on molecular markers did not agree with those from antioxidant activity. The paper also discusses the issues that still need to be addressed to utilize the full potential of molecular techniques including expressed sequence tag-polymerase chain reaction (EST-PCR) analysis to develop improved environment-friendly berry cultivars suited to the changing needs of growers and consumers.

Congruence between morphological and molecular markers for genetic diversity analysis applied to forage palm genotypes propagated via bioreactors

2020 ◽  
Vol 147 ◽  
pp. 112230
Author(s):  
Selma Silva Rocha ◽  
Luciana Cardoso Nogueira Londe ◽  
Samy Pimenta ◽  
Maurício Mendes Cardoso ◽  
Nívio Poubel Gonçalves ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Diversity Analysis ◽  
Genetic Diversity Analysis

Retrotransposon-based molecular markers for linkage and genetic diversity analysis in wheat

2003 ◽  
Vol 271 (1) ◽  
pp. 91-97 ◽  
Author(s):  
R. A. Queen ◽  
B. M. Gribbon ◽  
C. James ◽  
P. Jack ◽  
A. J. Flavell
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Diversity Analysis ◽  
Genetic Diversity Analysis

scholarly journals Genetic diversity analysis of Rose varieties from Grandiflora group based on ISSR molecular markers

2021 ◽  
Vol 38 ◽  
pp. 00140
Author(s):  
Sophia S. Yudanova ◽  
Svetlana A. Plugatar ◽  
Zinaida K. Klimenko ◽  
Vera K. Zykova ◽  
Olena L. Rubtsova ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Diversity Analysis ◽  
Morphometric Characters ◽  
Climate Conditions ◽  
Genetic Diversity Analysis ◽  
Statistical Comparison ◽  
Separate Branch ◽  
Issr Molecular Markers ◽  
Continental Climate

Kinship and genetic diversity determination among six rose varieties from the Grandiflora group were carried out using ISSR thechnics. The studied varities were divided into 3 clades: I) ‘Lezginka’, ‘Queen Elizabeth’ and ‘Koralovy surpriz’ varieties; II) ‘Gurzuf’ and ‘Love’ varieties; III) ‘Komsomolsky ogonek’ variety formed a separate branch. This division into clades was confirmed by a statistical comparison of morphometric characters. On the basis of the obtained data, it can be concluded that the analysis using the selected primer group is well-suited for differentiation rose varieties into groups, which makes it possible, for one thing, to determine the genetic distance between varieties, and for another, to use these data in the future by certification of the varieties promising by resistance characters to the continental climate conditions.

Genetic Diversity Analysis in Grevillea robusta using ISSR Molecular Markers

2019 ◽  
Vol 145 (3) ◽  
pp. 260
Author(s):  
Priyanka Parmar ◽  
Aman Dabral ◽  
R. K. Meena ◽  
Shailesh Pandey ◽  
Rama Kant ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Diversity Analysis ◽  
Grevillea Robusta ◽  
Genetic Diversity Analysis ◽  
Issr Molecular Markers
Sign in / Sign up

Export Citation Format

Share Document