scholarly journals Effect of ovarian types and collection techniques on the number of follicles and the quality of cumulus-oocyte-complexes in cow

2017 ◽  
Vol 45 (3) ◽  
pp. 10-16
Author(s):  
Khandoker MAMY ◽  
NF Atiqah ◽  
N Ariani
Keyword(s):  
Corpus Luteum ◽  
Recovery Rate ◽  
Normal Basis ◽  
Blunt Dissection ◽  
Embryo Production ◽  
Aspiration Technique ◽  
In Vitro Embryo Production ◽  
Dissection Technique

This experiment was conducted for evaluation of bovine slaughterhouse ovary, follicles, and cumulus-oocyte-complexes (COCs) and to compare the effect of collection techniques on the recovery rate of COCs.  The collected slaughterhouse ovaries was classified as corpus luteum present (CL+) and corpus luteum absent (CL-) groups.  It was found that 62.5% of the ovaries collected were CL- type and 37.5% were CL+ type.  For collection of COCs, blunt dissection and aspiration techniques were performed and number of follicles collected was recorded.  The higher number of follicles were dissected and aspirated from CL - ovaries (11.2±1.8and 37.8±14.9, respectively) compared to CL+ ovaries 10.5±1.5and 28.3±15.6, respectively).  The follicular materials collected from both techniques were observed under microscope to categorize the COCs as A (oocyte surrounded with cumulous cells homogenously), B (oocyte surrounded with cumulous cells partially), C (oocyte not surrounded at all by cumulous cells) and D (degeneration observed both in oocyte and cumulous cells).  Grade A and grade B were classified as normal and grade C and grade D were considered as abnormal COCs.  The result indicated that ovaries having no CL contributing more total number of COCs per ovary (6.8±1.0) and also contributing higher normal COCs (5.7±0.9) than that of ovaries with CL (6.0±2.0 and 4.5±1.5, respectively) in blunt dissection technique.  But same trend of result was not found in aspiration technique.  Similarly, higher percentage of COCs recovery rate was also recorded in blunt dissection (61.6±4.6% vs 16.5±4.9%, on total basis) than aspiration (48.6±2.9% vs 11.7±4.1%, on normal basis) technique.  So, ovaries without CL and blunt dissection technique found more suitable for harvesting the higher number and superior quality of COCs for extending the in vitro embryo production experiment.Bang. J. Anim. Sci. 2016. 45 (3): 10-16

scholarly journals Comparative seasonal assessment on the quality of Black Bengal goat oocytes in view of in vitro embryo production

2020 ◽  
Vol 5 (3) ◽  
pp. 78-87
Author(s):  
Shikha Sarkar ◽  
Md Saiful Islam ◽  
Md Enayet Kabir ◽  
Falguni Dadok ◽  
Md Abdullah Al Zaber ◽  
...  
Keyword(s):  
Corpus Luteum ◽  
Recovery Rate ◽  
In Vitro Maturation ◽  
Winter Season ◽  
Excellent Opportunity ◽  
Different Seasons ◽  
In Vitro Embryo Production ◽  
Black Bengal Goat

In vitro maturation (IVM) of oocytes provide an excellent opportunity for cheap and abundant embryos for carrying out animal improvement. With the aim for studying the quality of oocyte in different seasons of Black Bengal goat, both right and left ovaries were collected from the slaughter houses. For each of the specimens, gross parameters such as right, left, corpus luteum (CL)-present and corpus luteum (CL)-absent group were evaluated on the basis of weight (g), length (cm), width (cm), follicles aspirated and number and state of cumulus-oocyte-complexes (COCs), normal COCs and abnormal COCs, the effect of season on cumulus oocyte complexes (COCs) of goat oocytes. Our study revealed that the average number of follicles and oocytes recovery rate were higher in summer than in winter season and recovery rate of grade A and B oocytes were higher in summer than that of in winter and recovery rate of grade C and D were higher in winter than that of in summer. In the present study a significantly greater number of oocytes per ovary were recovered from ovaries without a corpus luteum than from ovaries with a corpus luteum. So the summer was the best season for recovery of COCs which have a worthy competence to be matured in vitro. Asian Australas. J. Biosci. Biotechnol. 2020, 5 (3), 78-87

143 Effect of corpus luteum on recovery rate, quality, and in vitro developmental competence of oocytes in Bos indicus dairy cows

2019 ◽  
Vol 31 (1) ◽  
pp. 196
Author(s):  
M. Saad ◽  
Z. Sarwar ◽  
M. Saleem ◽  
U. Arshad ◽  
M. Shahzad ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Corpus Luteum ◽  
Recovery Rate ◽  
Bos Indicus ◽  
Developmental Competence ◽  
Serum Concentrations ◽  
Embryo Production ◽  
The Mean ◽  
In Vitro Embryo Production

Assisted reproductive technologies have modernized the dairy industry for the rapid multiplication of superior genetic traits. However, the exploitation of genetics through in vitro embryo production in farm animals has been struggling during the last 3 decades. The presence or absence of a corpus luteum (CL) on ovaries from which cumulus-oocyte complexes were recovered has been shown to affect the quality of oocytes and in vitro embryo production outcome in Bos taurus cows. Similar information is lacking in Bos indicus cows. Therefore, the objectives of the present study were to determine the effect of presence or absence of a CL on recovery rate, quality, and in vitro developmental competence of oocytes in Bos indicus dairy cows. This study was conducted from December 2017 to April 2018. The ovaries were harvested from a local abattoir (Bos indicus; 5- to 8-year-old cows having mixed parity with clinically normal reproductive tracts). These ovaries (n=750) were divided into 2 groups [(1) CYCLIC (n=318) and (2) ACYCLIC (n=432)] based on the presence or absence of mature CL having follicles on either left or right ovaries of slaughtered cows. Blood samples from the jugular vein were collected at slaughter. Serum concentrations of progesterone of CYCLIC and ACYCLIC cows were measured using radioimmunoassay. Mean number of recovered oocytes per ovary and serum concentration of progesterone were compared using PROC TTEST model. However, quality, maturation, cleavage, 8-cell, 16-cell, and morula rates were analysed by PROC FREQ model of Chi-squared. All the data was analysed using SAS (SAS Institute Inc., Cary, NC, USA). Results revealed that the mean serum concentrations of progesterone (4.21±0.4v. 0.5±0.2ng mL−1; P<0.05) were higher in CYCLIC as compared with ACYCLIC cows, respectively. The mean number of oocytes recovered per ovary (6.5±4.5v. 4.0±4.4; P<0.05) was higher in CYCLIC than in ACYCLIC cows. The oocytes with grade I+II quality (55.3v. 47.6%; P<0.05) were higher, whereas oocytes with grade III+IV quality (44.5v. 52.4%; P<0.05) were lower, in CYCLIC as compared with ACYCLIC cows, respectively. Furthermore, cleavage rate (70.9v. 52.8%; P<0.05) was higher in CYCLIC than in ACYCLIC cows, respectively. Similarly, the 8-cell (38.5v. 20.8%; P<0.05) and 16-cell (20.0v. 10.9%; P<0.05) stage embryos were greater in CYCLIC as compared with ACYCLIC cows, respectively. In conclusion, the presence of CL has a beneficial effect on recovery rate, quality, and in vitro embryo production outcomes in Bos indicus dairy cows. It is implied that for ovum pickup, cyclic cows should be preferred.

Cleavage Rate of in vitro Matured Oocytes Fertilized with Conventional Semen in Buffaloes

2021 ◽  
Author(s):  
M.H. Pitroda ◽  
K.P. Khillare ◽  
M.B. Amle ◽  
M.D. Meshram ◽  
A.B. Mali ◽  
...  
Keyword(s):  
Fertilization Rate ◽  
Cleavage Rate ◽  
Embryo Production ◽  
Quick Recovery ◽  
Slaughter House ◽  
Maturation Rate ◽  
Aspiration Technique ◽  
Current Scenario ◽  
In Vitro Embryo Production

Background: In vitro embryo production in buffaloes has gained much importance in this current scenario due to ever increasing population and high demand of milk and meat. Slaughter house derived bubaline ovaries are a cheap and abundant source of cumulus oocyte complexes.Methods: Oocytes from the buffalo ovarian follicles were recovered by aspiration technique as it facilitates quick recovery. Total 155 ovaries were used in the present study. Surface follicles were measured using vernier calliper and categorized into three groups viz. less than 3 mm, 3-5 mm and greater than 5 mm based on follicular diameter and oocytes were processed for IVM, IVF and IVC using conventional non sorted semen.Result: Overall percentage of small, medium and large follicles in the ovaries were recorded as 16.29 ± 0.94%, 8.14±0.60%, 5.35 ± 0.76%, respectively. Overall recovery rate of COCs was 38%. The percentage of these oocytes were 16.74% (A), 15.25% (B), 25.26% (C), 18.33% (D) and 29.87% (E) respectively. Maturation rate of oocytes were 81.96 ± 2.70%. Fertilization rate was 74.98 ± 3.87%, Cleavage rate % was 40.84±2.51% and Blastocyst percentage was 21.57±1.75% respectively. Application of in vitro embryo production technique using slaughter house ovaries can salvage the genetic potential of bubaline species.

scholarly journals Synchronization with estradiol benzoate in the presence of the corpus luteum in Wagyu cows increases the number of medium follicles but does not interfere with in vitro production of embryos

2021 ◽  
Vol 42 (3) ◽  
pp. 1147-1158
Author(s):  
Maria Fernanda Zamai ◽  
◽  
Fábio Luiz Bim Cavalieri ◽  
Marcia Aparecida Andreazzi ◽  
Fabio Morotti ◽  
...  
Keyword(s):  
Corpus Luteum ◽  
Estradiol Benzoate ◽  
Breeding Systems ◽  
In Vitro Production ◽  
Embryo Production ◽  
Follicular Wave ◽  
In Vitro Embryo Production ◽  
Oocyte Donors ◽  
Vitro Production

Reproductive biotechnologies are emerging as an important element for livestock; however, some strategies must be modified to adapt to different breeding systems, such as the use of follicular synchronization protocols. This study aimed to evaluate follicular synchronization using estradiol benzoate (EB), in the presence of the corpus luteum (CL) from Wagyu oocyte donors in in vitro embryo production (IVEP). Rounds of IVEP were performed in heifers and cows (n=19) that were classified into three groups: G1/CL - animals with CL, G2/WCL - animals without CL, and G3/CL + EB - animals with CL that were subjected to follicular synchronization with EB at D0. The groups G1/CL and G2/WCL were considered the control and undertook the natural process of follicular dynamics. The results showed that the synchronization of the follicular wave with the application of EB in the presence of CL, presented a smaller number of small (6.05 ± 0.55) and large follicles (0.45 ± 0.15), but increased (P < 0.05) the number of medium-sized follicles (16.20 ± 0.90). However, the results of ovum pick up showed that regardless of whether or not EB was applied, and regardless of the presence or absence of CL in the Wagyu donor, there was no difference among the groups (P > 0.05) concerning the number of viable oocytes and the viability rate. It was concluded that follicular synchronization using EB in Wagyu oocyte donors that presented a CL, increased the number of medium-sized follicles. However, there was no improvement in the efficiency of ovum pick up, in vitro embryo production, and pregnancy rate.

scholarly journals Influence of Dominant Follicle and Corpus luteum on Recovery of Good Quality Oocytes for In vitro Embryo Production in Cattle

2014 ◽  
Vol 4 (12) ◽  
pp. 1305-1312 ◽  
Author(s):  
Jurandy Penitente-Filho ◽  
Erly Carrascal ◽  
Fabrício Oliveira ◽  
Adriana Zolini ◽  
Carlos Oliveira ◽  
...  
Keyword(s):  
Corpus Luteum ◽  
Dominant Follicle ◽  
Embryo Production ◽  
In Vitro Embryo Production

Intrinsic quality of goat oocytes already found denuded at collection for in vitro embryo production

2016 ◽  
Vol 86 (8) ◽  
pp. 1989-1998 ◽  
Author(s):  
Joanna Maria Gonçalves Souza-Fabjan ◽  
Yann Locatelli ◽  
Nicolas Duffard ◽  
Emilie Corbin ◽  
Ribrio Ivan Tavares Pereira Batista ◽  
...  
Keyword(s):  
Embryo Production ◽  
Intrinsic Quality ◽  
In Vitro Embryo Production

scholarly journals Kualitas Oosit Kerbau dari Status Reproduksi Ovarium yang Berlainan

2017 ◽  
Vol 35 (2) ◽  
pp. 216
Author(s):  
Sri Gustina ◽  
Hasbi Hasbi ◽  
Ni Wayan Kurniani Karja ◽  
Mohamad Agus Setiadi
Keyword(s):  
Reproductive Cycle ◽  
Average Weight ◽  
Embryo Production ◽  
Reproduction Cycle ◽  
Slaughter House ◽  
In Vitro Embryo Production ◽  
Quality Difference

In vitro embryo production relies on the quality of oocytes, however the quality is subjected to ovaries reproduction cycle. This study was conducted to observe the potency of buffalo ovaries from various reproductive cycle in producing quality oocytes. Collected pairs of ovaries from slaughter house were weighed and grouped of 5 according to the cycle. Oocytes were collected by slicing techniques, then selected based on quality. The results showed the average weight of buffalo ovaries of (+CL, +FD); (+CL, -FD); (-CL, +FD); (-CL, -FD) are 7.2 g; 5.5 g; 4.1 g; and 4.5 g respectively. No significant quality difference of produced oocytes between ovaries cycles (P>0.5). Good quality of collected oocytes were only 40-55%. Approximately 2-5 oocytes of grade A and 1-5 oocytes of grade B can be collected per pair of ovaries. 

scholarly journals Effect of quality of sperm chromatin structure on in -vitro production of cattle embryos

2005 ◽  
Vol 48 (1) ◽  
pp. 32-39
Author(s):  
L. Kątska-Książkiewicz ◽  
M. Bochenek ◽  
B. Ryńska
Keyword(s):  
Chromatin Structure ◽  
Blastocyst Stage ◽  
Sperm Chromatin ◽  
In Vitro Production ◽  
Embryo Production ◽  
In Vitro Embryo Production ◽  
Bull Ejaculate ◽  
Vitro Production

Abstract. Bull effect on results of in vitro embryo production has been well documented. The aim of the present study was to find the relationship between quality of bull sperm chromatin and its effect on in vitro embryo production. Bovine in vitro matured oocytes were fertilized in vitro using capacitated spermatozoa (freshly ejaculated or frozen-thawed) of 12 bulls. Semen was simultaneously processed according to the sperm chromatin structure assay (SCSA) method and was analysed by flow cytometry. At least 3 replications of IVP with the same semen sample were done. The percentage of spermatozoa with abnormal chromatin ranged from 0.4% to 23.8%. All bulls used for the experiment were divided into three groups showing minimal (0.82% ± 6.82%), low (1.70% ± 15.82%) and high (18.16% ± 53.59%) percentages of spermatozoa with abnormal chromatin structure. Both cleavage rates and embryo development to the blastocyst stage were correlated significantly with sperm chromatin abnormalities and resulted in 23.1, 17.7 and 12.2% of blastocysts respectively for sperm with minimal, low and high percentages of chromatin abnormalities. The SCSA method may be used as a practical indicator of suitability of bull ejaculate for IVP purposes.

293 EFFECT OF FOLLICULAR WAVE SYNCHRONIZATION ON IN VITRO EMBRYO PRODUCTION IN COWS

2006 ◽  
Vol 18 (2) ◽  
pp. 254
Author(s):  
A. F. Ramos ◽  
R. Rumpf ◽  
M. R. Mollo ◽  
J. U. Câmara ◽  
I. Pivato ◽  
...  
Keyword(s):  
Latin Square ◽  
Estradiol Benzoate ◽  
Continuous Variables ◽  
Cleavage Rate ◽  
Embryo Production ◽  
Follicular Wave ◽  
The Mean ◽  
In Vitro Embryo Production

In order to achieve the ideal time of ovum pickup (OPU) for in vitro embryo production (IVP) in cows regarding number and quality of oocytes recovered, this study investigated the effect of synchronization of wave emergence with estradiol benzoate (EB) injected 7 days prior to follicular aspiration. In a Latin square design, 12 crossbred beef cows were randomly divided into three groups, with three replicates each. Cows were synchronized with a norgestomet ear implant for 7 days followed by an i.m. prostuglandin F2� (PGF2�) injection and aspiration of all ovarian follicles larger than 3 mm in diameter. After that, follicles from cows in group 2X were aspirated twice a week with 4- and 3-day intervals, and follicles from groups 1X and 1X-EB were aspirated once a week. Cows from group 1X-EB also received an im injection of 2 mg of EB immediately after OPU. Throughout the study cows were kept with an ear norgestomet implant that was replaced every 2 weeks. Ultrasound evaluations of numbers of follicles greater than 3 mm in diameter and size of the largest follicle at the time of OPU were performed. Recovered oocytes were evaluated for quality, and the viable ones (Grades I, II, and III) were in vitro-fertilized on Day 0. Cleavage rate was evaluated on Day 2 and blastocyst production on Day 7. Continuous variables were compared by ANOVA and binomial data were compared by chi-square. For the 2X group, only data from the OPU performed 3 days after the last OPU were used for analysis. Results are presented as percentages or mean � SEM. Size of the largest follicle was greater (P < 0.05) in 1X coes (12.9 � 0.2 mm) than in 1X-EB cows (11.1 � 0.3 mm), which was greater than in 2X (9.6 � 0.4 mm) cows. The 1X cows had more follicles at OPU than 2X cows (17.5 � 0.7 vs. 14.1 � 0.9), whereas the 1X-EB group (15.9 � 0.7) was intermediate and not different from the others. There was no difference in the mean number of recovered oocytes among 2X (9.6 � 0.6), 1X (12.7 � 0.8) and 1X-EB (12.3 � 1.0) cows, and the mean number of viable oocytes among groups (5.8 � 0.5, 7.3 � 0.5, and 7.0 � 0.6) for 2X, 1X, and 1X-EB cows, respectively). The rate of viable oocytes was also similar among groups [58.8% (191/325) for 2X, 58.4% (267/457) for 1X, and 57.2% (231/404) for 1X-EB cows]. Cleavage [68.6% (131/191), 65.2% (174/267), and 68.4% (158/231)] and blastocyst [38.7% (74/191), 43.8% (117/267), and 44.2% (102/231)] rates were also not different among 2X, 1X, and 1X-EB groups, respectively. Although the use of 2 mg of EB in association with a norgestomet implant 7 days prior to OPU altered the follicular wave profile, it was not enough to improve number and quality of the oocytes recovered. Moreover, this study failed to demonstrate a positive effect of OPU earlier after wave emergence, when the effect of dominance should be less pronounced, on IVP in cows. The first author was supported by the fellowship 141077/2004-2 of CNPq, Brazil.

274 LAPAROSCOPIC OVUM PICK-UP IN SHEEP AND GOATS: EFFECTS OF REPEATED RECOVERIES AND FOLLICULAR DIAMETER

2006 ◽  
Vol 18 (2) ◽  
pp. 244
Author(s):  
A. Gibbons ◽  
F. Pereyra Bonnet ◽  
M. Cueto ◽  
M. Catala ◽  
D. Salamone
Keyword(s):  
Ovarian Stimulation ◽  
Recovery Rate ◽  
Follicular Development ◽  
Embryo Production ◽  
Recovery Rates ◽  
Sheep And Goats ◽  
Follicle Diameter ◽  
Follicular Size ◽  
In Vitro Embryo Production

Laparoscopic ovum pickup (LOPU) is a modern technique that may be used in programs of sheep and goat in vitro embryo production. The effects of repeated recoveries and follicular diameter on the efficiency of LOPU were evaluated in these species. In six Merino donor sheep and five Criolla goats, ovarian stimulation for follicular development was performed by administering a single dose of 60 mg NIH-FSH-P1 (Folltropin®; Bioniche, Canada) and 300 UI of eCG (Novoromon 5000®; Syntex, Argentina). Every four days, a total of 4 LOPU in sheep and 3 in goats were performed 24 h after each ovarian stimulation treatment. The intravaginal sponge with 60 mg of medroxyprogesterone (Esponjas Vaginales Syntex®; Syntex, Argentina) were removed after the last LOPU. Follicular fluid was aspirated under laparoscopic observation according to the follicle diameter with a cannula for intrauterine artificial insemination without a vacuum pump. The number and size of follicles, oocyte quantity and quality and recovery rate were evaluated. A total of 566 follicles were aspirated (14.4 ± 2.4 follicles/sheep and 14.6 ± 7.5 follicles/goat), yielding a total of 263 oocytes (6.8 ± 1.5 oocytes/sheep and 6.6 ± 2.9 oocytes/goat) and a recovery rate of 47% in sheep and 46% in goats (Table 1). In both species, there were no significant differences in the number of aspirated follicles, recovered oocytes, overall recovery rate, and the recovery rate of good quality oocytes in the repeated LOPU. The oocyte recovery rates in sheep differed significantly according to the follicular size (55% in large follicles, 36% in small follicles; P < 0.05), whereas in goats recovery rates of oocytes related to follicular size showed no significant differences (42 and 54% for large and small follicles, respectively). Follicular sizes from which oocytes were obtained was not a determining factor in the recovery rate of good quality oocytes. The technique used would allow effective and repeated oocyte recoveries for in vitro embryo production in sheep and goats. Table 1. Repeated recoveries by laparoscopic ovum pickup (LOPU) in sheep and goats

Sign in / Sign up

Export Citation Format

Share Document