Longitudinal monitoring in Cambodia suggests higher circulation of alpha and betacoronaviruses in juvenile and immature bats of three species

Recent studies suggest that coronaviruses circulate widely in Southeast Asian bat species and that the progenitors of the SARS-Cov-2 virus could have originated in rhinolophid bats in the region. Our objective was to assess the diversity and circulation patterns of coronavirus in several bat species in Southeast Asia. We undertook monthly live-capture sessions and sampling in Cambodia over 17 months to cover all phases of the annual reproduction cycle of bats and test specifically the association between their age and CoV infection status. We additionally examined current information on the reproductive phenology of Rhinolophus and other bat species presently known to occur in mainland southeast China, Vietnam, Laos and Cambodia. Results from our longitudinal monitoring (573 bats belonging to 8 species) showed an overall proportion of positive PCR tests for CoV of 4.2% (24/573) in cave-dwelling bats from Kampot and 4.75% (22/463) in flying-foxes from Kandal. Phylogenetic analysis showed that the PCR amplicon sequences of CoVs (n = 46) obtained clustered in Alphacoronavirus and Betacoronavirus. Interestingly, Hipposideros larvatus sensu lato harbored viruses from both genera. Our results suggest an association between positive detections of coronaviruses and juvenile and immature bats in Cambodia (OR = 3.24 [1.46–7.76], p = 0.005). Since the limited data presently available from literature review indicates that reproduction is largely synchronized among rhinolophid and hipposiderid bats in our study region, particularly in its more seasonal portions (above 16° N), this may lead to seasonal patterns in CoV circulation. Overall, our study suggests that surveillance of CoV in insectivorous bat species in Southeast Asia, including SARS-CoV-related coronaviruses in rhinolophid bats, could be targeted from June to October for species exhibiting high proportions of juveniles and immatures during these months. It also highlights the need to develop long-term longitudinal surveys of bats and improve our understanding of their ecology in the region, for both biodiversity conservation and public health reasons.

COMMUNICATIVE MODEL OF SERVICE-ORIENTED CUSTOMS ADMINISTRATION

. The article proposes and substantiates a communicative service-oriented model based on synchronization of reproduction cycles of the subjects of customs administration. The reproduction cycle is considered as the basic model of the communication process between the service provider and the service recipient; the process is based on the customs service, which is transformed within the reproduction cycle from a product of customs authorities into a production resource of a participant in FEA.

The study of the consequences of preserving berry crops in vitro on the processes of subsequent clonal micropropagation

As a result of studies, it was found that collection samples of berry crops in vitro can be maintained in viable condition on specially selected nutrient media for 12 months. The effect of mannitol concentration (0.45, 0.75 and 1.05%; sucrose control -3%) on the regenerative potential of microprobe of the studied berry crop varieties was estimated. When studying the effect of the reproduction cycle on the morphogenesis of meristemic cultures, it was found that the dynamics of changes in shoot length, number of leaves and shoots depended on the concentration of the active substance. In general, the majority of berry crops that were viable after storage of explants retained the ability to grow again, in many cases reaching or exceeding the control level.

CULTURAL MEANINGS OF THE PRODUCTION-CONSUMPTION CYCLE

The article explores the cultural meanings of the classical reproduction cycle ‘production-consumption’. The purpose of the article is to analyse the implicit semantic component of the processes of production and consumption, in which the value symbolism of the joint existence of people objectified. The empirical material for the analysis is classical and modern economic and sociological conceptual ideas that represent the cultural meanings of the reproduction cycle.

An Integrated Approach to the Analysis of the Reproductive Potential of a Machine-building Enterprise in the Formation of Its Product Policy

An integrated approach to the analysis of the potential of the phases and participants of the reproduction process at the stage of forming the product policy of a machine-building enterprise and its development in critical directions is proposed. This allows, from a unified standpoint on the mismatch between the target and the achieved level of potential, to identify critical participants in the reproduction cycle, whose internal capabilities are insufficient to optimize the economic result from the product program and to identify the contribution of each participant based on the analysis of the hierarchy of cause-and-effect relationships of the variables of the utility function with the elements of their potential, as well as assess and select local development strategies for critical participants. The materials of the article can be useful to specialists dealing with the problems of developing a product policy and developing enterprises.