scholarly journals Study of the Effect of Sodium Dodecyl Sulfate (SDS) and Acridine Orange on the Isolation of Plasmid and Antimicrobial Resistance Pattern of Clinical Isolates of Klebsiella Sp

1970 ◽  
Vol 7 (1) ◽  
pp. 38-43
Author(s):  
Mohammad Shahriar ◽  
Shumaila Mawla ◽  
Mohiuddin Ahmed Bhuiyan ◽  
Mahboob Hossain
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Acridine Orange ◽  
Antimicrobial Susceptibility ◽  
Sodium Dodecyl ◽  
Luria Broth ◽  
Multidrug Resistant ◽  
Plasmid Profile ◽  
Resistance Pattern ◽  
Antimicrobial Susceptibility Pattern ◽  
Dodecyl Sulfate

For a six months period a total of 25 Klebisiella sp. samples collected from different diagnostic centers were tested for their antimicrobial susceptibility pattern against 16 antimicrobials. All isolates under study showed resistance against cephalexin, cephradine, ciporfloxacin, cloxacillin, erythromycin, oxacillin, rifampicin and tetracycline. Most of the isolates were resistant to amoxicillin (92%), vancomycin (96%), neomycin (84%) and chloramphenicol (76%). Least number of isolates showed resistance to tetracycline (36%), ceftriaxone (40%) and gentamycin (56%), respectively. Out of the 25 isolates, 7 multidrug resistant isolates were selected and they were grown in varying concentrations of Sodium Dodecyl Sulfate (SDS) in Luria broth and their plasmid profile was analysed. Distinct bands of plasmid were observed when the multidrug resistant strains of Klebsiella sp. were grown in 5% SDS and acridine orange (10μg/ml) in Luria broth. On being treated with 5% SDS and 5% SDS along with acridine orange, one isolate (isolate no. 3) developed sensitivity against ceftriaxone, ciprofloxacin, co-trimoxazole, gentamycin and neomycin. Keywords: Klebsiella sp.; plasmid; antimicrobial susceptibility; multidrug resistance; sodium dodecyl sulfate (SDS)   DOI: http://dx.doi.org/10.3329/diujst.v7i1.9646   Daffodil International University Journal of Science and Technology Vol.7(1) 2012 38-43

scholarly journals Effect of Sodium Dodecyl Sulfate and Acridine Orange on Isolation of Plasmid and Antimicrobial Resistance Pattern of Clinical Isolates of Klebsiella sp.

2012 ◽  
Vol 4 (2) ◽  
pp. 499 ◽  
Author(s):  
M. Shahriar ◽  
S. Mawla ◽  
S. M. A. Islam ◽  
M. A. Bhuiyan
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Acridine Orange ◽  
Sodium Dodecyl ◽  
Luria Broth ◽  
Multidrug Resistant ◽  
Plasmid Profile ◽  
Resistance Pattern ◽  
Susceptibility Pattern ◽  
Time Period ◽  
Dodecyl Sulfate

The susceptibility pattern of 25 Klebsiella sp. samples, collected for a six months time period from diagnostic centers, was investigated against 16 different antimicrobials. All the isolates of this study exhibit resistance against cephalexin, cephradine, ciporfloxacin, cloxacillin, erythromycin, oxacillin, rifampicin and tetracycline. Most of the isolates were resistant to amoxicillin (92%), vancomycin (96%), neomycin (84%) and chloramphenicol (76%). A few of the isolates showed resistance to tetracycline (36%), ceftriaxone (40%) and gentamycin (56%). Seven multidrug resistant (MDR) out of the 25 isolates were selected and grown in varying concentrations of sodium dodecyl sulfate (SDS) in Luria broth. Their plasmid profile was also analyzed. Distinct plasmid bands were observed when the MDR strains of Klebsiella sp. were grown in 5% SDS and acridine orange (10 µg/ml) in Luria broth. One of the isolates (isolate no. 3) treated with 5% SDS and/or 5% SDS along with acridine orange developed sensitivity against ceftriaxone, ciprofloxacin, co-trimoxazole, gentamycin and neomycin. Keywords: Plasmid; Antimicrobial susceptibility; Multidrug resistant; Sodium dodecyl sulfate (SDS). © 2012 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved. doi: http://dx.doi.org/10.3329/jsr.v4i2.8445 J. Sci. Res. 4 (2), 499-505 (2012) 

scholarly journals Plasmid Curing of Escherichia coli Cells with Ethidium Bromide, Sodium Dodecyl Sulfate and Acridine Orange

1970 ◽  
Vol 27 (1) ◽  
pp. 28-31 ◽  
Author(s):  
MA Zaman ◽  
MH Pasha ◽  
MZ Akhter
Keyword(s):  
Escherichia Coli ◽  
Sodium Dodecyl Sulfate ◽  
Acridine Orange ◽  
Ethidium Bromide ◽  
Sodium Dodecyl ◽  
Drug Resistant ◽  
Plasmid Curing ◽  
Wild Type ◽  
Dodecyl Sulfate ◽  
I Cells

The plasmid eliminating abilities of acridine orange, ethidium bromide and sodium dodecyl sulfate were investigated on multi drug resistant Escherichia coli from urinary tract infection specimens. Three different concentrations of each curing agent (Et-Br, SDS and AO) were used. The frequencies of cured cells were 5.55 % (with 50 μg/ml) and 11.76 % (with 75 μg/ml) for acridine orange, 14.29 % (with 100 μg/ml), 21.05 % (with 100 μg/ ml), 17.65 % (with 125 μg/ml) for ethidium bromide and 7.4 % (with 10 % w/v) & 6.67 % (with 10 % w/v) for sodium dodecyl sulfate. However, no cured cells were obtained from 100 μg/ml acridine orange, 75 μg/ml ethidium bromide and 8 and 12 % SDS. Analysis of profiles of wild type and plasmid cured strains by electrophoresis yielded bands of varying sizes for wild type cells, but none were obtained for Et-Br cured cells. Acridine orange treated cells could eliminate only plasmids of 2.7 MDa and another smaller than 2 MDa. Key Words: Plasmid curing; Escherichia coli; Ethidium Bromide; Sodium Dodecyl Sulfate; Acridine Orange. DOI: http://dx.doi.org/10.3329/bjm.v27i1.9165 BJM 2010; 27(1): 28-31

FLUORESCENCE LIFETIME OF ACRIDINE ORANGE IN SODIUM DODECYL SULFATE PREMICELLAR SOLUTIONS

1988 ◽  
Vol 47 (5) ◽  
pp. 685-688 ◽  
Author(s):  
Norio Miyoshi ◽  
Kiyoaki Hara ◽  
Issei Yokoyama ◽  
Giiti Tomita ◽  
Masaru Fukuda
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Acridine Orange ◽  
Fluorescence Lifetime ◽  
Sodium Dodecyl ◽  
Dodecyl Sulfate

scholarly journals Antibiotic resistance pattern and its correlation to the presence of tdh gene and CRISPR-Cas system in Vibrio parahaemolyticus strains isolated from seafood

2019 ◽  
Vol 66 (2) ◽  
Author(s):  
Pallavi Baliga ◽  
Malathi Shekar ◽  
Shaik Thahur Ahamed ◽  
M. N. Venugopal
Keyword(s):  
Antibiotic Resistance ◽  
Negative Correlation ◽  
Antimicrobial Susceptibility ◽  
Vibrio Parahaemolyticus ◽  
Multidrug Resistant ◽  
Resistance Pattern ◽  
Susceptibility Pattern ◽  
Antimicrobial Susceptibility Pattern ◽  
Thermostable Direct Hemolysin ◽  
Tdh Gene

Vibrio parahaemolyticus is a pathogen native to the aquatic environment. In this study, 46 environmental V. parahaemolyticus isolates were subjected to a correlational analysis to find the association between their antimicrobial susceptibility pattern, prevalence of CRISPR-Cas system and thermostable direct hemolysin (tdh) gene. Antibiotic resistance profiling against eleven antibiotics revealed the isolates to be multidrug resistant. Isolates exhibited highest resistance to vancomycin (97.8%) followed by ampicillin (91.3%), cefotaxime (69.6%), ceftazidime/clavulanic acid (54.4%), ceftazidime (45.7%) and gentamicin (39.1%). CRISPR loci and tdh gene were detected in 47.83 and 58.7% of strains respectively. No significant correlation was observed between antibiotic resistance to presence of CRISPR, except in the case of gentamicin wherein, a negative correlation was seen (r=-0.272, p<0.10). Similarly, tdh did not correlate to antibiotic resistance. Seventeen strains in this study harboured the CRISPR loci as well as tdh gene, the association of which was found to be statistically significant.

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