scholarly journals Antibiotic resistance pattern and its correlation to the presence of tdh gene and CRISPR-Cas system in Vibrio parahaemolyticus strains isolated from seafood

2019 ◽  
Vol 66 (2) ◽  
Author(s):  
Pallavi Baliga ◽  
Malathi Shekar ◽  
Shaik Thahur Ahamed ◽  
M. N. Venugopal
Keyword(s):  
Antibiotic Resistance ◽  
Negative Correlation ◽  
Antimicrobial Susceptibility ◽  
Vibrio Parahaemolyticus ◽  
Multidrug Resistant ◽  
Resistance Pattern ◽  
Susceptibility Pattern ◽  
Antimicrobial Susceptibility Pattern ◽  
Thermostable Direct Hemolysin ◽  
Tdh Gene

Vibrio parahaemolyticus is a pathogen native to the aquatic environment. In this study, 46 environmental V. parahaemolyticus isolates were subjected to a correlational analysis to find the association between their antimicrobial susceptibility pattern, prevalence of CRISPR-Cas system and thermostable direct hemolysin (tdh) gene. Antibiotic resistance profiling against eleven antibiotics revealed the isolates to be multidrug resistant. Isolates exhibited highest resistance to vancomycin (97.8%) followed by ampicillin (91.3%), cefotaxime (69.6%), ceftazidime/clavulanic acid (54.4%), ceftazidime (45.7%) and gentamicin (39.1%). CRISPR loci and tdh gene were detected in 47.83 and 58.7% of strains respectively. No significant correlation was observed between antibiotic resistance to presence of CRISPR, except in the case of gentamicin wherein, a negative correlation was seen (r=-0.272, p<0.10). Similarly, tdh did not correlate to antibiotic resistance. Seventeen strains in this study harboured the CRISPR loci as well as tdh gene, the association of which was found to be statistically significant.

Virulence and Antibiotic Resistance of Vibrio parahaemolyticus Isolates from Seafood from Three Developing Countries and of Worldwide Environmental, Seafood, and Clinical Isolates from 2000 to 2017

2017 ◽  
Vol 80 (12) ◽  
pp. 2060-2067 ◽  
Author(s):  
Mohammad M. Obaidat ◽  
Alaa E. Bani Salman ◽  
Amira A. Roess
Keyword(s):  
Antibiotic Resistance ◽  
Nalidixic Acid ◽  
Vibrio Parahaemolyticus ◽  
Multidrug Resistant ◽  
Middle Income ◽  
Middle Income Countries ◽  
Fish Samples ◽  
Thermostable Direct Hemolysin ◽  
Third Generation Cephalosporins ◽  
Tdh Gene

ABSTRACT Vibrio parahaemolyticus is a leading cause of seafood-associated illness. This study investigated the prevalence, virulence, and antibiotic resistance of V. parahaemolyticus in three low- and middle-income countries. Freshly caught fish samples (n = 330) imported to Jordan from Yemen, India, and Egypt were tested. The overall prevalence of V. parahaemolyticus was 15% (95% confidence interval: 11 to 19%). Three isolates (6%) were positive for the thermostable direct hemolysin–related (trh) gene, and all isolates was negative for the thermostable direct hemolysin (tdh) gene. All isolates were resistant to colistin sulfate, neomycin, and kanamycin, and 51 and 43% of isolates were resistant to tetracycline and ampicillin, respectively. Only 4% of the isolates were resistant to cefotaxime and chloramphenicol, and no isolates were resistant to sulfamethoxazole-trimethoprim, streptomycin, gentamicin, ciprofloxacin, and nalidixic acid. All isolates were resistant to two classes of antibiotics, and 86% were multidrug resistant (resistant to at least one drug in three or more classes of antibiotics). A literature review of clinical, seafood, and environmental V. parahaemolyticus isolates worldwide revealed high rates of gentamicin and ampicillin resistance, emerging resistance to third-generation cephalosporins, and limited resistance to sulfamethoxazole-trimethoprim, ciprofloxacin, nalidixic acid, and chloramphenicol. Thus, last-resort antibiotics could be ineffective for treating V. parahaemolyticus infections. Several global reports also documented illness outbreaks in humans caused by trh- and tdh-negative V. parahaemolyticus strains. More research is needed to determine whether the presence of these genes is sufficient to classify the strains as virulent.

scholarly journals Bacteriological profile and antimicrobial susceptibility pattern of endotracheal tube secretion of patients in ICUs of a tertiary care hospital in Punjab

2021 ◽  
Vol 8 (3) ◽  
pp. 224-229
Author(s):  
Kiranjeet Kaur ◽  
Shavetika Jindal
Keyword(s):  
Antimicrobial Susceptibility ◽  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Multidrug Resistant ◽  
Diffusion Method ◽  
Care Hospital ◽  
Susceptibility Pattern ◽  
Prospective Longitudinal Study ◽  
Antimicrobial Susceptibility Pattern ◽  
Prospective Longitudinal

Healthcare associated pneumonia (HAP)is second most common HCAIs that occur in 27% critically ill patients. Eighty-six percent of HAP are associated with mechanical ventilation and termed as ventilator associated pneumonia (VAP). VAP due to multidrug resistant has also increased in recent past. To isolate and identify the bacterial pathogens in endotracheal tubes aspirates of ICUs patients and study their antimicrobial susceptibility pattern.A prospective longitudinal study was conducted in the Microbiology laboratory of a tertiary care hospital over a period of six months after clearance from institutional Research Committee and Ethical Committee. All the samples of ETT secretions received in Clinical Microbiology lab from ICU patients and fulfilling the criteria for VAP were included in this study. Samples were processed as per standard protocol and organisms were identified on the basis of gram staining, colony characters and biochemical tests. Antibiotic sensitivity was performed by Kirby Bauer disc diffusion method as per CLSI guidelines. : A total of 100 samples of ET secretions were collected and proceeded for culture. Out of 100 samples, 76 (76.0%) were positive for bacterial growth. Among 76 positive cultures, a total 80 bacterial isolates were obtained as some cultures were showing polymicrobial growth. Five (6.26%) isolates were Gram Positive bacteria and 75(93.7%) were Gram negative. The most frequent isolates were 35(43.7%) followed by , isolates were sensitive to colistin while resistant to ampicillin and amoxiclav. isolates were sensitive to colistin and resistant to ampicillin, amoxiclav, ciprofloxacin, cefixime, piperacillin tazobactam. isolates were sensitive to colistin while resistant to ampicillin, amoxiclav, ceftizidime and piperacillin tazobactam. In our study antimicrobial pattern of isolated bacteria shows multidrug resistant pathogens which are associated with VAP and limit therapeutic options.

Prevalence of Multidrug-Resistant Salmonella on Commercial Dairies Utilizing a Single Heifer Raising Facility†

2008 ◽  
Vol 71 (1) ◽  
pp. 27-34 ◽  
Author(s):  
TOM S. EDRINGTON ◽  
TODD R. CALLAWAY ◽  
ROBIN C. ANDERSON ◽  
DAVID J. NISBET
Keyword(s):  
United States ◽  
Antibiotic Resistance ◽  
Dairy Cattle ◽  
Antimicrobial Susceptibility ◽  
Susceptibility Testing ◽  
Brilliant Green ◽  
Dairy Farm ◽  
Multidrug Resistant ◽  
Resistance Pattern ◽  
Lactating Cows

The objectives of the current research were twofold: (i) to determine the prevalence of multidrug-resistant (MDR) Salmonella in the various classes of dairy cattle and (ii) to determine if comingling of calves from multiple farms at a heifer feedlot serves as a transmission vector for Salmonella back to the dairy farm. Four large commercial dairies in the southwestern United States were sampled in October 2005 and again in March 2006. Fecal samples were collected from hutch calves, 12-and 24-month-old heifers, lactating cows, dry cows, and cattle in the sick-fresh pen and cultured with brilliant green agar supplemented with novobiocin (BGAnov) to estimate the overall Salmonella prevalence, or with tetracycline (BGAtet) to estimate MDR Salmonella. Antimicrobial susceptibility testing was conducted with the National Antibiotic Resistance Monitoring System (NARMS) testing panel, and a portion of the isolates were serotyped. Salmonella prevalence among groups ranged from 0 to 96% positive, with the highest incidence observed in the hutch calves and cattle in the sick-fresh pen. Twenty-eight different serotypes were identified with serotype Reading accounting for the majority of isolates cultured on BGAtet. Nearly all (100 of 103) isolates cultured on BGAnov and screened for antibiotic resistance were pan susceptible, whereas over one-half (64%) of the isolates cultured on BGAtet were MDR. Forty isolates displayed the ACSSuT resistance pattern, and 36 isolates displayed the MDR-AmpC pattern of the 72 isolates examined following culture on BGAtet. The incidence of Salmonella cultured on BGAtet was low (9%) in all heifers and only one MDR isolate was cultured (from a 12-month-old heifer), suggesting the risk of transmission of Salmonella from the heifer feedlot back to the dairy is low. Results of this research suggest the incidence of MDR Salmonella, found primarily in hutch calves and cattle in the sick-fresh pen, is low in comparison to the overall Salmonella prevalence.

scholarly journals Escherichia coli O157:H7 in Retail Lettuce (Lactuca sativa) in Addis Ababa City: Magnitude of Contamination and Antimicrobial Susceptibility Pattern

2021 ◽  
Vol 12 ◽  
Author(s):  
Aklilu Feleke Haile ◽  
Silvia Alonso ◽  
Nega Berhe ◽  
Tizeta Bekele Atoma ◽  
Prosper N. Boyaka ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Susceptibility ◽  
Addis Ababa ◽  
Foodborne Pathogen ◽  
Multidrug Resistant ◽  
Diffusion Method ◽  
Susceptibility Pattern ◽  
Disk Diffusion Method ◽  
E Coli ◽  
Antimicrobial Susceptibility Pattern

Escherichia coli O157:H7 is an important foodborne pathogen but largely under investigated in Africa. The objectives of this study were to estimate the prevalence and pattern of antimicrobial resistance of E. coli O157:H7 in lettuce in Addis Ababa, Ethiopia. A total of 390 retail lettuce samples were collected across the 10 subcities of Addis Ababa. E. coli O157:H7 was isolated and identified following ISO-16654:2001 standard. The isolates were further tested for antimicrobial susceptibility to 13 antimicrobials using the Kirby–Bauer disk diffusion method. Out of the 390 lettuce samples examined, two (0.51%) carried E. coli O157:H7. The antimicrobial susceptibility pattern of strains showed resistance to ampicillin (100%) and tetracycline (50.0%). One of the two isolates was multidrug resistant to two antimicrobials tested. The results of this study demonstrate the presence of drug-resistant E. coli O157:H7 in lettuce in markets in Addis Ababa. Despite the low prevalence, its presence in a product that is eaten raw highlights potential public health risk in the area associated with this pathogen.

scholarly journals Isolation and Antimicrobial Susceptibility Pattern of Urinary Escherichia Coli in Dhaka Medical College Hospital, Bangladesh

2017 ◽  
Vol 7 (1) ◽  
pp. 40-44
Author(s):  
Tashmin Afroz Binte Islam ◽  
SM Shamsuzzaman ◽  
Irin Rahman ◽  
Faria Ferdousi
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Susceptibility ◽  
Medical College Hospital ◽  
Resistance Pattern ◽  
Susceptibility Pattern ◽  
Biochemical Tests ◽  
Test Reaction ◽  
College Hospital ◽  
Medical College ◽  
Antimicrobial Susceptibility Pattern

Background: Escherichia coli is the major bacterial pathogen being isolated and reported from urine, globally. For these uropathogens the therapeutic management becomes limited, so knowledge about the antimicrobial resistance pattern of these agents at a specific area may help the doctors to choose correct treatment regimen. The aim of the current study was to detect the common uropathogens from urine and the antimicrobial susceptibility pattern of Escherichia coli in Dhaka Medical College Hospital (DMCH).Methods: Samples were collected over a period of 12 months from July 2011 to June 2012 from the patients of DMCH irrespective of age and sex. Samples were cultured in blood agar and MacConkey agar media and organisms were identified by different biochemical tests such as oxidase test, reaction in MIU and simmon's citrate media and different sugar fermentation tests. Data were analyzed using Microsoft Excel (2007).Results: From total 300 urine samples, 177 (59%) Gram-positive and Gram-negative bacteria were isolated. Among them, Esch. coli was the most predominant 112 (63.28%) organism followed by Enterobacter spp. (11.87%). The prevalence of Esch. coli was significantly higher in females (63.39%) of younger age group than in males (36.61%). Esch. coli was highly sensitive to imipenem (100%) followed by ceftriaxone (62.50%), ceftazidime (55.36%) and azitromycin (35.72%) respectively. Low sensitivity patterns were found against doxycycline (13.39%), co-trimoxazole (16.07%), amoxiclav (21.43%) and ciprofloxacin (23.22%) respectively.Conclusion: The result of this study provides insight into the high proportion of multidrug resistant Escherichia coli and creates a need to report them routinely in laboratories.Anwer Khan Modern Medical College Journal Vol. 7, No. 1: Jan 2016, P 40-44

scholarly journals Study of the Effect of Sodium Dodecyl Sulfate (SDS) and Acridine Orange on the Isolation of Plasmid and Antimicrobial Resistance Pattern of Clinical Isolates of Klebsiella Sp

1970 ◽  
Vol 7 (1) ◽  
pp. 38-43
Author(s):  
Mohammad Shahriar ◽  
Shumaila Mawla ◽  
Mohiuddin Ahmed Bhuiyan ◽  
Mahboob Hossain
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Acridine Orange ◽  
Antimicrobial Susceptibility ◽  
Sodium Dodecyl ◽  
Luria Broth ◽  
Multidrug Resistant ◽  
Plasmid Profile ◽  
Resistance Pattern ◽  
Antimicrobial Susceptibility Pattern ◽  
Dodecyl Sulfate

For a six months period a total of 25 Klebisiella sp. samples collected from different diagnostic centers were tested for their antimicrobial susceptibility pattern against 16 antimicrobials. All isolates under study showed resistance against cephalexin, cephradine, ciporfloxacin, cloxacillin, erythromycin, oxacillin, rifampicin and tetracycline. Most of the isolates were resistant to amoxicillin (92%), vancomycin (96%), neomycin (84%) and chloramphenicol (76%). Least number of isolates showed resistance to tetracycline (36%), ceftriaxone (40%) and gentamycin (56%), respectively. Out of the 25 isolates, 7 multidrug resistant isolates were selected and they were grown in varying concentrations of Sodium Dodecyl Sulfate (SDS) in Luria broth and their plasmid profile was analysed. Distinct bands of plasmid were observed when the multidrug resistant strains of Klebsiella sp. were grown in 5% SDS and acridine orange (10μg/ml) in Luria broth. On being treated with 5% SDS and 5% SDS along with acridine orange, one isolate (isolate no. 3) developed sensitivity against ceftriaxone, ciprofloxacin, co-trimoxazole, gentamycin and neomycin. Keywords: Klebsiella sp.; plasmid; antimicrobial susceptibility; multidrug resistance; sodium dodecyl sulfate (SDS)   DOI: http://dx.doi.org/10.3329/diujst.v7i1.9646   Daffodil International University Journal of Science and Technology Vol.7(1) 2012 38-43

scholarly journals Characterization and Analysis of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPRs) in Pandemic and Non-Pandemic Vibrio parahaemolyticus Isolates from Seafood Sources

2021 ◽  
Vol 9 (6) ◽  
pp. 1220
Author(s):  
Nawaporn Jingjit ◽  
Sutima Preeprem ◽  
Komwit Surachat ◽  
Pimonsri Mittraparp-arthorn
Keyword(s):  
Antibiotic Resistance ◽  
Antimicrobial Susceptibility ◽  
Hemolytic Activity ◽  
Vibrio Parahaemolyticus ◽  
Sequence Type ◽  
Multiple Antibiotic Resistance ◽  
Hemolysin Gene ◽  
Shared Ancestry ◽  
Thermostable Direct Hemolysin ◽  
Mar Index

Vibrio parahaemolyticus is one of the significant seafood-borne pathogens causing gastroenteritis in humans. Clustered regularly interspaced short palindromic repeats (CRISPR) are commonly detected in the genomes of V. parahaemolyticus and the polymorphism of CRISPR patterns has been applied as a genetic marker for tracking its evolution. In this work, a total of 15 pandemic and 36 non-pandemic V. parahaemolyticus isolates obtained from seafood between 2000 and 2012 were characterized based on hemolytic activity, antimicrobial susceptibility, and CRISPR elements. The results showed that 15/17 of the V. parahaemolyticus seafood isolates carrying the thermostable direct hemolysin gene (tdh+) were Kanagawa phenomenon (KP) positive. The Multiple Antibiotic Resistance (MAR) index ranged between 0.1 and 0.4, and 45% of the isolates have an MAR index ≥ 0.2. A total of 19 isolates were positive for CRISPR detection, including all tdh+ trh− isolates, two of tdh− trh+, and each of tdh+ trh+ and tdh− trh−. Four spacer types (Sp1 to Sp4) were identified, and CRISPR-positive isolates had at least one type of spacer homolog to the region of Vibrio alginolyticus megaplasmid. It is of interest that a specific CRISPR profile and spacer sequence type was observed with correlations to the hemolysin genotype (tdh/trh). Thus, these provide essential data on the exposure of foreign genetic elements and indicate shared ancestry within different genotypes of V. parahaemolyticus isolates.

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