scholarly journals Study of Genetic Variation in Different Hatchery Populations of Common Carp (Cyprinus carpio) of Mymensingh District in Bangladesh using Microsatellite DNA Markers

1970 ◽  
Vol 14 ◽  
pp. 113-120 ◽  
Author(s):  
MAS Jewel ◽  
MM Rahman ◽  
MN Islam
Keyword(s):  
Genetic Variation ◽  
Genetic Distance ◽  
Common Carp ◽  
Cyprinus Carpio ◽  
Dna Markers ◽  
Microsatellite Dna ◽  
Mirror Carp ◽  
Microsatellite Dna Markers ◽  
Pcr Products ◽  
High Level

Genetic variation of two strains (Scaled and Mirror) of six hatchery populations of common carp (Cyprinus carpio) was studied using three microsatellite loci (MFW13, MFW17 and MFW28). The loci were amplified by Polymerase Chain Reaction (PCR). The PCR products were electrophoresed on 6% polyacrylamide gel and the alleles were visualized by silver nitrate staining. All the three loci were found polymorphic. The average number of alleles was the highest in the population *SC-SMK (5.00) and lowest in **MC-SMK (4.00). Total number of null allele was more in mirror carp populations (6) than scaled carp populations (4). The average observed heterozygosity (Ho) ranged from 0.54 to 0.67. The average observed heterozygosity (Ho) was lower than the average expected heterozygosity (He) in case of all the populations. The population ***SC-BCH had high level of genetic variation in comparison to other five populations because this population had negative 1-Ho/He values in case of two loci out of three analyzed. The rest five populations had negative 1-Ho/He values only in one locus. In 6 of a total of 18 tests, significant deviations from Hardy-Weinberg expectations (HWE) were detected. Pairwise Fst values were used to detect the genetic distance between the pairs of populations. The Fst value was highest (0.153) between the population MC-AFF and MC-SMK. Nei’s genetic distance value was also highest (0.462) between these populations. The Fst value of 0.153 represents a high level of population differentiation. The lowest Fst value (0.001) was found between SC-SMK and MC-SMK. This value was statistically insignificant. *SC–SMK- scaled carp from Sagor Matsho Khamar **MC – SMK- mirror carp from Sagor Matsho Khamar ***SC-BCH- scaled carp from Brahmaputra Char Fishery Key words: Genetic variation, common carp, Microsatellite DNA markers J. bio-sci. 14: 113-120, 2006

Genetic Variation of Anastrepha suspensa (Diptera: Tephritidae) in Florida and the Caribbean Using Microsatellite DNA Markers

2010 ◽  
Vol 103 (6) ◽  
pp. 2214-2222 ◽  
Author(s):  
Laura M. Boykin ◽  
Robert G. Shatters ◽  
David G. Hall ◽  
David Dean ◽  
Peter Beerli
Keyword(s):  
Genetic Variation ◽  
Dna Markers ◽  
Microsatellite Dna ◽  
Anastrepha Suspensa ◽  
Microsatellite Dna Markers ◽  
The Caribbean

scholarly journals Genetic variability of the fifth generation of nile tilapia Oreochromis niloticus using microsatellite DNA markers

2015 ◽  
Vol 10 (2) ◽  
pp. 124
Author(s):  
Gloria Ika Satriani ◽  
Dinar Tri Soelistyowati ◽  
Dian Hardianto ◽  
Ratu Siti Aliah
Keyword(s):  
Genetic Variability ◽  
Genetic Distance ◽  
Dna Markers ◽  
Microsatellite Loci ◽  
Microsatellite Dna ◽  
Nile Tilapia ◽  
Fifth Generation ◽  
Freshwater Aquaculture ◽  
Microsatellite Dna Markers ◽  
Aquaculture Development

<p>ABSTRACT</p><p><br />Fifth generations of Nile tilapia from several strains have been produced by using selective breeding program in Main Centre for Freshwater Aquaculture Development (MCFAD) Sukabumi, West Java. This research was aimed to evaluate the impact of family selection program of some highly economic traits on its genetic variability using microsatellite DNA markers. The total of 180 specimens have been collected from fifth generation of nine reciprocal mating between three families selected from fourth generation of Nile tilapia and were screened for genetic variability at three microsatellite loci (UNH 123*, UNH 172*, UNH 216*). The results showed that the amount of genetic variability on fifth generations of Nile tilapia from three strains was ranged between 33 to 100% and the highest genetic distance relationship between families was 0.3875. This research approved that females and males issued from the family which have more amount of genetic variability and higher distance to others could be considered as genetic materials to produce the next generation.</p><p><br />Keywords: microsatellite DNA, genotype, genetic variability, genetic distance, Oreochromis nilotiocus</p><p><br /> ABSTRAK</p><p><br />Beberapa strain ikan nila generasi kelima telah dihasilkan dalam program pemuliaan di Main Centre untuk Freshwater Aquaculture Development (MCFAD) Sukabumi, Barat Jawa. Riset ini bertujuan untuk mengevaluasi pengaruh seleksi famili terhadap performa karakter ekonomis penting berdasarkan keragaman genetiknya menggunakan penanda microsatellite DNA. Spesimen dari 180 individu generasi kelima hasil persilangan resiprokal antara tiga famili generasi keempat dianalisis dengan penanda tiga microsatellite loci (UNH 123*, UNH 172*, UNH 216*). Hasil penelitian menunjukkan bahwa keragaman genetik ikan nila generasi kelima berkisar antara 33 sampai 100% dan hubungan kekerabatan genetik antar famili yang paling jauh adalah 0,3875. Individu betina dan jantan yang berasal dari famili dengan tingkat keragaman genetik dan kekerabatan yang lebih tinggi dapat dipertimbangkan sebagai sumber genetik berkualitas untuk menghasilkan generasi berikutnya.<br />Kata kunci: microsatellite DNA, genotipe, keragaman genetik, jarak genetik, Oreochromis nilotiocus</p>

scholarly journals Development of Novel Microsatellite DNA Markers by Cross-Amplification and Analysis of Genetic Variation in Gerbils

2010 ◽  
Vol 101 (6) ◽  
pp. 710-716 ◽  
Author(s):  
Xiaoyan Du ◽  
Zhenwen Chen ◽  
Wei Li ◽  
Yuanqing Tan ◽  
Jing Lu ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Dna Markers ◽  
Microsatellite Dna ◽  
Cross Amplification ◽  
Microsatellite Dna Markers

Genetic variation in farmed populations of the gilthead sea bream Sparus aurata in Greece using microsatellite DNA markers

2011 ◽  
Vol 43 (2) ◽  
pp. 239-246 ◽  
Author(s):  
Dimitrios Loukovitis ◽  
Elena Sarropoulou ◽  
Emmanouella Vogiatzi ◽  
Costas S. Tsigenopoulos ◽  
Georgios Kotoulas ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Dna Markers ◽  
Microsatellite Dna ◽  
Sparus Aurata ◽  
Gilthead Sea Bream ◽  
Sea Bream ◽  
Microsatellite Dna Markers

Preliminary studies on the genetic variability of six Hungarian common carp strains using microsatellite DNA markers

Hydrobiologia ◽  
2005 ◽  
Vol 533 (1-3) ◽  
pp. 223-228 ◽  
Author(s):  
István Lehoczky ◽  
István Magyary ◽  
Csaba Hancz ◽  
Steven Weiss
Keyword(s):  
Genetic Variability ◽  
Common Carp ◽  
Dna Markers ◽  
Microsatellite Dna ◽  
Microsatellite Dna Markers
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