scholarly journals Genetic variability of the fifth generation of nile tilapia Oreochromis niloticus using microsatellite DNA markers

2015 ◽  
Vol 10 (2) ◽  
pp. 124
Author(s):  
Gloria Ika Satriani ◽  
Dinar Tri Soelistyowati ◽  
Dian Hardianto ◽  
Ratu Siti Aliah
Keyword(s):  
Genetic Variability ◽  
Genetic Distance ◽  
Dna Markers ◽  
Microsatellite Loci ◽  
Microsatellite Dna ◽  
Nile Tilapia ◽  
Fifth Generation ◽  
Freshwater Aquaculture ◽  
Microsatellite Dna Markers ◽  
Aquaculture Development

<p>ABSTRACT</p><p><br />Fifth generations of Nile tilapia from several strains have been produced by using selective breeding program in Main Centre for Freshwater Aquaculture Development (MCFAD) Sukabumi, West Java. This research was aimed to evaluate the impact of family selection program of some highly economic traits on its genetic variability using microsatellite DNA markers. The total of 180 specimens have been collected from fifth generation of nine reciprocal mating between three families selected from fourth generation of Nile tilapia and were screened for genetic variability at three microsatellite loci (UNH 123*, UNH 172*, UNH 216*). The results showed that the amount of genetic variability on fifth generations of Nile tilapia from three strains was ranged between 33 to 100% and the highest genetic distance relationship between families was 0.3875. This research approved that females and males issued from the family which have more amount of genetic variability and higher distance to others could be considered as genetic materials to produce the next generation.</p><p><br />Keywords: microsatellite DNA, genotype, genetic variability, genetic distance, Oreochromis nilotiocus</p><p><br /> ABSTRAK</p><p><br />Beberapa strain ikan nila generasi kelima telah dihasilkan dalam program pemuliaan di Main Centre untuk Freshwater Aquaculture Development (MCFAD) Sukabumi, Barat Jawa. Riset ini bertujuan untuk mengevaluasi pengaruh seleksi famili terhadap performa karakter ekonomis penting berdasarkan keragaman genetiknya menggunakan penanda microsatellite DNA. Spesimen dari 180 individu generasi kelima hasil persilangan resiprokal antara tiga famili generasi keempat dianalisis dengan penanda tiga microsatellite loci (UNH 123*, UNH 172*, UNH 216*). Hasil penelitian menunjukkan bahwa keragaman genetik ikan nila generasi kelima berkisar antara 33 sampai 100% dan hubungan kekerabatan genetik antar famili yang paling jauh adalah 0,3875. Individu betina dan jantan yang berasal dari famili dengan tingkat keragaman genetik dan kekerabatan yang lebih tinggi dapat dipertimbangkan sebagai sumber genetik berkualitas untuk menghasilkan generasi berikutnya.<br />Kata kunci: microsatellite DNA, genotipe, keragaman genetik, jarak genetik, Oreochromis nilotiocus</p>

ANALYSIS OF GENETIC STRUCTURE OF DOGS OF FRENCH BULLDOG BREED USING MICROSATELLITE DNA MARKERS

2018 ◽  
Vol 51 ◽  
pp. 185-192
Author(s):  
S. Kruhlyk ◽  
V. Dzitsiuk ◽  
V. Spyrydonov
Keyword(s):  
Genetic Diversity ◽  
Genetic Variability ◽  
Dna Markers ◽  
Microsatellite Dna ◽  
High Variability ◽  
Molecular Diagnostic ◽  
Allelic Variants ◽  
Microsatellite Dna Markers ◽  
Gene Complexes ◽  
French Bulldog

Genetic variability of domestic dogs is a source for effective process of breed formation and creating unique gene complexes. In the world, for preservation of genetic resources of dogs, there are dog training associations which have great confidence: American Club Dog Breeders (AKC), the British Kennel Club (KC) and the Federation Cynologique Internationale (FCI), aimed at protecting breeding dogs, standards creation, registration of a breed, and issuance of accurate pedigrees. Evaluation of the genetic diversity of dog breeds is able to significantly complement and improve their breeding programs. Since breeds of dog differ in morphological and economic characteristics, the problem of finding of the breed features in the genome of animals is becoming more topical. From this point of view, French Bulldog is an interesting breed of dog (FRANC.BULLDOGGE, FCI standard number 101) which belongs by the classification of breeds, adopted in FCI, to the group IX – a dog-companion for health and fun, but to a subgroup of fighting dogs of a small format. French Bulldog breed has been researched slightly not only in Ukraine and also abroad, as the main work of all dog association is focused on solving theoretical and practical issues of breeding, keeping, feeding, veterinary protection and others. The study was conducted at Research Department of Molecular Diagnostic Tests of Ukrainian Laboratory of Quality and Safety of Agricultural Products. 33 animals of French Bulldog breed, admitted to use in dog breeding of Ukrainian Kennel Union (UKU), were involved for the genetic analysis using DNA markers. The materials of the research were buccal epithelial cells, selected before the morning feeding of animals by scraping mucous membrane of oral cavity with disposable, dry, sterile cotton swab. Genomic DNA was extracted using KIT-set of reagents for DNA isolation according to the manufacturer's instructions. PEZ1, PEZ3, PEZ6, PEZ8, FHC 2010, FHC 2054 markers, recommended by International Society for Animal Genetics (ISAG), ACN, КC and FCI, were used for research. As a result of research 25 alleles for all the loci were detected in the experimental sample of dogs. The average number of alleles at the locus Na, obtained by direct counting, was 4.16. The most polymorphic loci for this breed were PEZ6 and PEZ3 with 8 and 6 allelic variants. Monomorphic loci were PEZ8 and FHC 2054 which had 4 and 3 alleles and the lowest level of polymorphism was observed for PEZ 1 and FHC 2010 loci in which only 2 alleles were identified. On analyzing the molecular genetic characteristics of dogs of French Bulldog breed, we found a high variability of genotype on rare alleles, which included alleles: M, C, D, E, J, K, L, O, N and representing 60% of the total number of the identified alleles. C, D, E alleles for PEZ3 locus and O allele at PEZ6 locus are unique to the sampling of dogs because they are not repeated in other loci. Typical alleles: N, F, R, I, P, K, M are 40% of the total. But F, R alleles for PEZ3 locus and P allele for locus PEZ6 are not repeated either in standard allelic variants or in rare one, indicating a high information content of these alleles and loci to be used for further monitoring of allele pool, genetic certification and identification of dogs. Microsatellite DNA loci were analyzed as a result of investigations of French Bulldogs and the most informative: PEZ3, PEZ6 and PEZ8 were found, which have high efficiency in individual and breed certification of dogs due to high variability. These data allow further monitoring of the state of genetic diversity of the breed and the development of measures for improvement of breeding to preserve the structure of breeding material. The study of individual and population genetic variability is advisable to continue for breeding of French Bulldogs "in purity" and preserving valuable gene complexes. The results are the basis for further monitoring of the proposed informative panels of microsatellite DNA markers for genotyping dog of French Bulldog breed and their complex evaluation.

P4001 Molecular analysis of genetic variability in Egyptian buffalo using microsatellite DNA markers

2016 ◽  
Vol 94 (suppl_4) ◽  
pp. 80-80
Author(s):  
S. Abou Bakr ◽  
M. Attia ◽  
A. A. Nigm ◽  
S. Abdelghany ◽  
N. Abdallah
Keyword(s):  
Genetic Variability ◽  
Molecular Analysis ◽  
Dna Markers ◽  
Microsatellite Dna ◽  
Microsatellite Dna Markers ◽  
Egyptian Buffalo

scholarly journals Interspecies and intraspecies genetic diversity of Ongole Grade cattle and Madura cattle based on microsatellite DNA markers

2018 ◽  
Vol 19 (4) ◽  
pp. 1593-1600
Author(s):  
SUTARNO . ◽  
NINA KURNIANINGRUM ◽  
ELISA HERAWATI ◽  
AHMAD DWI SETYAWAN
Keyword(s):  
Genetic Diversity ◽  
Dna Sequences ◽  
Dna Markers ◽  
Microsatellite Loci ◽  
Microsatellite Dna ◽  
Polymorphic Information Content ◽  
Entire Genome ◽  
Genetic Characteristics ◽  
Microsatellite Dna Markers ◽  
The Mean

Sutarno, Kurnianingrum N, Herawati E, Setyawan AD. 2018. Interspecies and intraspecies genetic diversity of Ongole Gradecattle and Madura cattle based on microsatellite DNA markers. Biodiversitas 19: 1593-1600. DNA microsatellite has been extensivelyemployed for estimating the degree of kinship between genotypes and improving the quality of cattle products. Microsatellite markersare short-patterned DNA sequences and repeated tandem (sequentially) with 2-5 nucleotide units scattering the entire genome. Thepurpose of this study was to investigate the genetic characteristics of inter and intraspecies of Ongole Grade cattle and Madura cattleusing microsatellite DNA markers. Blood samples from 20 individuals of each species were extracted by the method referring to WizardGenomic DNA Purification Kit (Promega, USA) and PCR amplification was performed using 5 microsatellite loci, i.e., BM1824,ETH225, INRA005, MM12, and TGLA227. Results of the genetic characteristics of both species were calculated using the POPGENEprogram version 1.31. The data suggest that there is a genetic diversity of inter and intraspecies of Ongole Grade cattle and Maduracattle. The average value of Shannon's Information Index (I) at all microsatellite loci for Ongole Grade cattle was 0.76 and for Maduracattle was 1.12. Meanwhile, the average interspecies I value was 1.03. The mean intraspecies Polymorphic Information Content (PIC) ofOngole Grade and Madura cattle was 0.43, and 0.63, respectively, and the mean interspecies PIC value was 0.57. The data altogethersuggest that all loci meet the standards as being informative markers in the assessment of genetic population because it has a PIC value>0.5 especially for intraspecies of Madura cattle.

scholarly journals Study of Genetic Variation in Different Hatchery Populations of Common Carp (Cyprinus carpio) of Mymensingh District in Bangladesh using Microsatellite DNA Markers

1970 ◽  
Vol 14 ◽  
pp. 113-120 ◽  
Author(s):  
MAS Jewel ◽  
MM Rahman ◽  
MN Islam
Keyword(s):  
Genetic Variation ◽  
Genetic Distance ◽  
Common Carp ◽  
Cyprinus Carpio ◽  
Dna Markers ◽  
Microsatellite Dna ◽  
Mirror Carp ◽  
Microsatellite Dna Markers ◽  
Pcr Products ◽  
High Level

Genetic variation of two strains (Scaled and Mirror) of six hatchery populations of common carp (Cyprinus carpio) was studied using three microsatellite loci (MFW13, MFW17 and MFW28). The loci were amplified by Polymerase Chain Reaction (PCR). The PCR products were electrophoresed on 6% polyacrylamide gel and the alleles were visualized by silver nitrate staining. All the three loci were found polymorphic. The average number of alleles was the highest in the population *SC-SMK (5.00) and lowest in **MC-SMK (4.00). Total number of null allele was more in mirror carp populations (6) than scaled carp populations (4). The average observed heterozygosity (Ho) ranged from 0.54 to 0.67. The average observed heterozygosity (Ho) was lower than the average expected heterozygosity (He) in case of all the populations. The population ***SC-BCH had high level of genetic variation in comparison to other five populations because this population had negative 1-Ho/He values in case of two loci out of three analyzed. The rest five populations had negative 1-Ho/He values only in one locus. In 6 of a total of 18 tests, significant deviations from Hardy-Weinberg expectations (HWE) were detected. Pairwise Fst values were used to detect the genetic distance between the pairs of populations. The Fst value was highest (0.153) between the population MC-AFF and MC-SMK. Nei’s genetic distance value was also highest (0.462) between these populations. The Fst value of 0.153 represents a high level of population differentiation. The lowest Fst value (0.001) was found between SC-SMK and MC-SMK. This value was statistically insignificant. *SC–SMK- scaled carp from Sagor Matsho Khamar **MC – SMK- mirror carp from Sagor Matsho Khamar ***SC-BCH- scaled carp from Brahmaputra Char Fishery Key words: Genetic variation, common carp, Microsatellite DNA markers J. bio-sci. 14: 113-120, 2006

Preliminary studies on the genetic variability of six Hungarian common carp strains using microsatellite DNA markers

Hydrobiologia ◽  
2005 ◽  
Vol 533 (1-3) ◽  
pp. 223-228 ◽  
Author(s):  
István Lehoczky ◽  
István Magyary ◽  
Csaba Hancz ◽  
Steven Weiss
Keyword(s):  
Genetic Variability ◽  
Common Carp ◽  
Dna Markers ◽  
Microsatellite Dna ◽  
Microsatellite Dna Markers

scholarly journals Validation of clonal line females for sex determination studies in nile tilapia Oreochromis niloticus L.

2015 ◽  
Vol 1 (1) ◽  
pp. 147-158
Author(s):  
Mohd Golam Quader Khan ◽  
Brendan J McAndrew ◽  
David J Penman
Keyword(s):  
Sex Determination ◽  
Dna Markers ◽  
Oreochromis Niloticus ◽  
Microsatellite Dna ◽  
Nile Tilapia ◽  
Clonal Line ◽  
Nile Tilapia Oreochromis Niloticus ◽  
Microsatellite Dna Markers ◽  
Sequencing Studies ◽  
Sexually Mature

Sex determination in the Nile tilapia Oreochromis niloticus is more complex than a simple XX-XY sex determining mechanism, as evidenced from fairly frequent unexpected sex ratios in progeny. The production of uniform, homozygous experimental material is particularly advantageous for studying sex determining mechanism as well as for the genetic mapping and genome sequencing studies in which interpretations are facilitated by homozygosity. To better understand the genetic mechanism of sex determination, a fully inbred line of clonal females (XX) was verified in controlled environmental conditions using test crosses and microsatellite DNA markers from the tilapia linkage map. A total of successfully amplified 87 microsatellite DNA markers covering all 24 linkage groups were selected for screening sexually mature females from this line. 67 markers were found polymorphic in outbred individuals screened. Markers from LG1, LG3 and LG23 were given more emphasis because sex determining genes have been mapped on these LGs in different species of tilapia. The verification and validation of this clonal line of females made them an important resource to use as a ‘standard reference line’ in genomics, sex determination studies and other studies in Nile tilapia. DOI: http://dx.doi.org/10.3329/ralf.v1i1.22378 Res. Agric., Livest. Fish.1(1): 147-158, Dec 2014

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