Temporal Variation in Toxic Bait, Carbohydrate and Protein Preference and Toxic Bait Efficacy in Argentine (Linepithema Humile) and Darwin’s Ants (Doleromyrma Darwiniana)

<p>By determining if there is any temporal variation in toxic bait, carbohydrate and protein preference in Argentine and Darwin’s ants, we can provide better control options because we can identify with more accuracy when ants will be foraging for one food type over another. Improving our understanding is also fundamental as we can improve future bait formulations, bait application and timing, and increase levels of bait uptake. I have two aims in this thesis. First, I investigated toxic bait, carbohydrate and protein preferences for Argentine ants (Linepithema humile) and Darwin's ant (Doleromyrma darwiniana). The second aim in my thesis was to examine the efficacy of toxic baits in laboratory colonies of Argentine ants. Food preferences varied between species and within species considerably throughout the year, although Darwin's ant consistently favoured foods higher in carbohydrates. Argentine ants showed a significant preference for protein over carbohydrates during December and January. Despite differences in carbohydrate and protein preferences the preference for individual toxic baits showed little temporal variation. The toxic bait efficacy experiment revealed that Xstinguish™ and Exterm-an-Ant® baits produced the highest mortality rate. Other commercially available toxic baits had little effect on workers or queens. The efficacy of the toxic baits was influenced by the starvation level of the ant colonies, and only the colonies that were starved for 48 hours experienced a 100% mortality rate. Due to these results, I recommend that bait application with Xstinguish™ and Exterm-an-Ant® in late winter-spring would be optimal as this time frame is when ants are likely to be starved, and when foraging activity is increasing, thus maximising the chances of bait uptake. A second round of baiting treatment with both baits (Xstinguish™ and Exterm-an-Ant®) in summer when Argentine ants have been shown to undergo a second wave of reproduction could also be beneficial.</p>

Temporal Variation in Toxic Bait, Carbohydrate and Protein Preference and Toxic Bait Efficacy in Argentine (Linepithema Humile) and Darwin’s Ants (Doleromyrma Darwiniana)

<p>By determining if there is any temporal variation in toxic bait, carbohydrate and protein preference in Argentine and Darwin’s ants, we can provide better control options because we can identify with more accuracy when ants will be foraging for one food type over another. Improving our understanding is also fundamental as we can improve future bait formulations, bait application and timing, and increase levels of bait uptake. I have two aims in this thesis. First, I investigated toxic bait, carbohydrate and protein preferences for Argentine ants (Linepithema humile) and Darwin's ant (Doleromyrma darwiniana). The second aim in my thesis was to examine the efficacy of toxic baits in laboratory colonies of Argentine ants. Food preferences varied between species and within species considerably throughout the year, although Darwin's ant consistently favoured foods higher in carbohydrates. Argentine ants showed a significant preference for protein over carbohydrates during December and January. Despite differences in carbohydrate and protein preferences the preference for individual toxic baits showed little temporal variation. The toxic bait efficacy experiment revealed that Xstinguish™ and Exterm-an-Ant® baits produced the highest mortality rate. Other commercially available toxic baits had little effect on workers or queens. The efficacy of the toxic baits was influenced by the starvation level of the ant colonies, and only the colonies that were starved for 48 hours experienced a 100% mortality rate. Due to these results, I recommend that bait application with Xstinguish™ and Exterm-an-Ant® in late winter-spring would be optimal as this time frame is when ants are likely to be starved, and when foraging activity is increasing, thus maximising the chances of bait uptake. A second round of baiting treatment with both baits (Xstinguish™ and Exterm-an-Ant®) in summer when Argentine ants have been shown to undergo a second wave of reproduction could also be beneficial.</p>

Review and Meta-Analysis of the Evidence for Choosing between Specific Pyrethroids for Programmatic Purposes

Pyrethroid resistance is widespread in malaria vectors. However, differential mortality in discriminating dose assays to different pyrethroids is often observed in wild populations. When this occurs, it is unclear if this differential mortality should be interpreted as an indication of differential levels of susceptibility within the pyrethroid class, and if so, if countries should consider selecting one specific pyrethroid for programmatic use over another. A review of evidence from molecular studies, resistance testing with laboratory colonies and wild populations, and mosquito behavioural assays were conducted to answer these questions. Evidence suggested that in areas where pyrethroid resistance exists, different results in insecticide susceptibility assays with specific pyrethroids currently in common use (deltamethrin, permethrin, α-cypermethrin, and λ-cyhalothrin) are not necessarily indicative of an operationally relevant difference in potential performance. Consequently, it is not advisable to use rotation between these pyrethroids as an insecticide-resistance management strategy. Less commonly used pyrethroids (bifenthrin and etofenprox) may have sufficiently different modes of action, though further work is needed to examine how this may apply to insecticide resistance management.

Efficacy of chlorfenapyr- and hydramethylnon-based baits on laboratory colonies of the pharaoh ant

The effectiveness of insecticidal baits based on chlorfenapyr and hydramethylnone against colonies of the Pharaoh ant has been studied. Sugar baits containing 0.1–1.0 % chlorfenapyr are ineffective because after 4 weeks of the experiment, the viability of the colonies was not changed. Sugar baits based on 1 % hydramethylnon effectively destroyed workers, the effect on queens was weak, and depletion of the colony was achieved. Commercially produced baits effectively and completely destroy ant colonies both in the absence and in the presence of alternative food. Keywords: Pharaoh ant, baits, chlorfenapyr, hydramethylnon.

Efficacy of Anystis baccarum against Foxglove Aphids, Aulacorthum solani, in Laboratory and Small-Scale Greenhouse Trials

A generalist predatory mite, Anystis baccarum (L.), has been identified as a key predator of small, soft-bodied pest species in various agroecosystems around the world. The foxglove aphid Aulacorthum solani (Keltenbach) is a new problematic pest in Canadian greenhouses. Laboratory colonies of A. baccarum were established and its predatory efficacy against A. solani was assessed. In laboratory trials, A. baccarum ate approximately one adult aphid or seven first instar aphids in 24 h. In a greenhouse bench trial on sweet peppers with the free-flying aphid parasitoid, Aphidius ervi Haliday, the population dynamics of A. solani in the presence or absence of A. baccarum was evaluated. Although the parasitoid alone successfully eradicated A. solani, when A. baccarum were present on the plants, the aphid population was eradicated more rapidly. Fruit yield was also 15% higher from plants where A. baccarum was released than the control (without A. baccarum). Furthermore, plants were naturally infested by Frankliniella occidentalis (Pergande) during the trial, which caused visible feeding damage to the fruits. Anystis baccarum also predates on thrips and thrips’ feeding damage to the fruits was reduced on plants where A. baccarum was released. Anystis baccarum was able to establish in sweet peppers and was determined to be complementary to the current practice of using A. ervi for the biological control of A. solani.

Interactions of the Insect-Specific Palm Creek Virus with Zika and Chikungunya Viruses in Aedes Mosquitoes

Palm Creek virus (PCV) is an insect-specific flavivirus that can interfere with the replication of mosquito-borne flaviviruses in Culex mosquitoes, thereby potentially reducing disease transmission. We examined whether PCV could interfere with arbovirus replication in Aedes (Ae.) aegypti and Ae. albopictus mosquitoes, major vectors for many prominent mosquito-borne viral diseases. We infected laboratory colonies of Ae. aegypti and Ae. albopictus with PCV to evaluate infection dynamics. PCV infection was found to persist to at least 21 days post-infection and could be detected in the midguts and ovaries. We then assayed for PCV–arbovirus interference by orally challenging PCV-infected mosquitoes with Zika and chikungunya viruses. For both arboviruses, PCV infection had no effect on infection and transmission rates, indicating limited potential as a method of intervention for Aedes-transmitted arboviruses. We also explored the hypothesis that PCV–arbovirus interference is mediated by the small interfering RNA pathway in silico. Our findings indicate that RNA interference is unlikely to underlie the mechanism of arbovirus inhibition and emphasise the need for empirical examination of individual pairs of insect-specific viruses and arboviruses to fully understand their impact on arbovirus transmission.

Comprehensive Ecological and Geographic Characterization of Eukaryotic and Prokaryotic Microbiomes in African Anopheles

Exposure of mosquitoes to numerous eukaryotic and prokaryotic microbes in their associated microbiomes has probably helped drive the evolution of the innate immune system. To our knowledge, a metagenomic catalog of the eukaryotic microbiome has not been reported from any insect. Here we employ a novel approach to preferentially deplete host 18S ribosomal RNA gene amplicons to reveal the composition of the eukaryotic microbial communities of Anopheles larvae sampled in Kenya, Burkina Faso and Republic of Guinea (Conakry). We identified 453 eukaryotic operational taxonomic units (OTUs) associated with Anopheles larvae in nature, but an average of 45% of the 18S rRNA sequences clustered into OTUs that lacked a taxonomic assignment in the Silva database. Thus, the Anopheles microbiome contains a striking proportion of novel eukaryotic taxa. Using sequence similarity matching and de novo phylogenetic placement, the fraction of unassigned sequences was reduced to an average of 4%, and many unclassified OTUs were assigned as relatives of known taxa. A novel taxon of the genus Ophryocystis in the phylum Apicomplexa (which also includes Plasmodium) is widespread in Anopheles larvae from East and West Africa. Notably, Ophryocystis is present at fluctuating abundance among larval breeding sites, consistent with the expected pattern of an epidemic pathogen. Species richness of the eukaryotic microbiome was not significantly different across sites from East to West Africa, while species richness of the prokaryotic microbiome was significantly lower in West Africa. Laboratory colonies of Anopheles coluzzii harbor 26 eukaryotic OTUs, of which 38% (n = 10) are shared with wild populations, while 16 OTUs are unique to the laboratory colonies. Genetically distinct An. coluzzii colonies co-housed in the same facility maintain different prokaryotic microbiome profiles, suggesting a persistent host genetic influence on microbiome composition. These results provide a foundation to understand the role of the Anopheles eukaryotic microbiome in vector immunity and pathogen transmission. We hypothesize that prevalent apicomplexans such as Ophryocystis associated with Anopheles could induce interference or competition against Plasmodium within the vector. This and other members of the eukaryotic microbiome may offer candidates for new vector control tools.

The Mechanics of Social Interactions Between Cats and Their Owners

This is a mini review that summarizes what is known from quantitative observational studies of social interactions between domestic cats and humans in both laboratory colonies and the home setting. Only results from data that have been statistically analyzed are included; hypotheses still to be tested will be declared as such. In some cases, the observational data have been combined with independently collected subjective assessments by the owners of the animals' character and owner personality traits to help interpret the data. Further some relevant experimental studies are also included. All social interactions between cats and humans that are discussed below assume that the animals were socialized to people as kittens, the first topic of this review. Such socialized cats show what might be called “friendliness to humans,” which in turn affects human attachment to the cat. The visual and acoustic behavioral elements used to communicate and interact with other cats can be perceived by people and are also employed by the cats when interacting with them. The initiation, and the initiator of social interactions between cats and humans have been shown to influence both the duration of the interaction bout and total interaction time in the relationship. Compliance with the interactional “wishes” of the partner is positively correlated between the cats and the humans over all human-cat dyads examined. Cats do not spontaneously prefer one gender or age cohort of people, but the humans in those cohorts behave differently to the cats causing the latter to react differentially. The dyadic interaction structure has also been shown to differ between women and men and between older and younger adults. Nevertheless, cats—merely their presence but of course their behavior—can affect human moods and human mood differences have been shown to affect the behavior of the cats. Finally, differences have been found between interactions with purebred and non-purebred cats and between younger and older cats.

Reproductive and Developmental Biology of Acroclisoides sinicus, a Hyperparasitoid of Scelionid Parasitoids

Acroclisoides sinicus (Hymenoptera: Pteromalidae) was described in 1988 from China, but recent findings in Europe and North America within the framework of Halyomorpha halys (Hemiptera: Pentatomidae) biological control indicate a Holarctic distribution. The few records and fragmented information on A. sinicus are derived from generic observations of other species belonging to the same genus, and its biological and ethological traits are still completely unexplored. It was suspected to be a facultative or obligate hyperparasitoid of many egg parasitoid species (e.g., Scelionidae and Eupelmidae), especially those parasitizing Pentatomidae eggs. Laboratory colonies of A. sinicus were established from specimens collected in the field in Europe and the USA, which allowed us to investigate for the first time the life traits of this somewhat enigmatic species. Our studies confirmed the obligate hyperparasitoid hypothesis for species of Scelionidae but not of Eupelmidae. Laboratory studies revealed that A. sinicus is extremely selective in its host recognition as only the pupal stage of its host species is exploited for parasitization. Taking into consideration its hyperparasitoid habit, the adventive A. sinicus populations in Europe and North America may potentially be severe threats to pentatomid natural control as new components in the trophic chain of pentatomids and their parasitoid guilds.

Auditory perception of self and others in zebra finches: evidence from an operant discrimination task

Vocal communication is essential for social interactions in many animal species. For this purpose an animal has to perceive vocal signals of conspecifics and is often also required to discriminate conspecifics. The capacity to discriminate conspecifics is particularly important in social species in which individuals interact repeatedly. In addition, auditory perception of self plays an important role for vocal learners. It allows a vocal learner to memorise vocalisations of conspecifics and to subsequently modify its own vocalisations in order to match the memorised vocalisations. Here we investigated auditory perception of self and others in zebra finches (Taeniopygia guttata), a highly gregarious songbird species and vocal learner. We used laboratory colonies in which founder males had been previously trained to produce the same song type. This resulted in artificial dialects in the song of founders and their offspring. We investigated whether those birds would be able to discriminate between familiar and unfamiliar conspecifics based on song. Furthermore, we examined whether they would classify their own song as familiar or unfamiliar. We found that birds were able to discriminate between songs of familiar versus unfamiliar conspecifics, despite the fact that all songs were imitations of the same song type. This suggests that such discrimination is possible even based on songs with a high acoustic similarity. None of the subjects classified their own song as unfamiliar. Three out of eight males classified their own song as familiar. Thus zebra finches might recognise their own song. Further experiments are needed to confirm such self-recognition.