scholarly journals Plantlet Regeneration from Seedling Explants of Sweet Gourd

2014 ◽  
Vol 18 (2) ◽  
pp. 61-66
Author(s):  
J Akter ◽  
MS Haque ◽  
MA Karim
Keyword(s):  
Callus Induction ◽  
Plantlet Regeneration ◽  
Direct Regeneration ◽  
Root Tip ◽  
Ms Medium ◽  
Cucurbita Moschata ◽  
Shoot Induction ◽  
Cotyledon Explants ◽  
Shoot Differentiation ◽  
Seedling Explants

To regenerate plantlets both directly and indirectly, different explants (cotyledon, hypocotyl, root tip and shoot tip) of sweet gourd (Cucurbita moschata) were cultured on medium supplemented with different concentrations and combinations of NAA (0.0, 0.2, 0.5 and 1.0 mg/L) and BAP (0.0, 0.5, 2.0 and 5.0 mg/L). Cotyledon explants performed best in callus induction. The combination of 5 mg/L BAP and 0.2 mg/L NAA produced the highest callus frequency in cotyledon (90.0%). The calli derived from cotyledon, hypocotyl and root tip were cultured in MS medium supplemented with different concentrations of kinetin, BAP and/or NAA for shoot induction. Regeneration via callus was achieved only from cotyledon calli at a frequency of 65.0% on 2 mg/L BAP and 0.2 mg/L NAA. Shoot tips cultured for direct regeneration, in the same media containing 1 mg/L BAP or 1 mg/L BAP + 0.2 mg/L NAA resulted in 100.0% shoot differentiation. The regenerated shoots rooted on MS medium with or without 0.1 mg/L NAA (100%).DOI: http://dx.doi.org/10.3329/pa.v18i2.18093 Progress. Agric. 18(2): 61 - 66, 2007

scholarly journals In vitro Regeneration of Mungbean (Vigna radiata L.) from Different Explants

2013 ◽  
Vol 19 (2) ◽  
pp. 13-19 ◽  
Author(s):  
MK Khatun ◽  
MS Haque ◽  
S Islam ◽  
KM Nasiruddin
Keyword(s):  
Callus Induction ◽  
Vigna Radiata ◽  
Shoot Tip ◽  
Direct Regeneration ◽  
Root Tip ◽  
Ms Medium ◽  
Shoot Induction ◽  
Cotyledon Explants ◽  
Shoot Differentiation

To regenerate plantlets both directly and indirectly, different explants (cotyledon, hypocotyls, root tip and shoot tip) of mungbean were cultured on medium supplemented with different concentrations and combinations of BAP (0, 1.0 and 5.0 mgL-1) and NAA (0, 0.5 and 2.5 mgL-1)). Cotyledon explants performed best in callus induction (90.0%) at the combination of 1 mgL-1) BAP and 2.5 mgL-1)NAA for both the varieties (BINA mung 5 and BINA mung 7). The calli derived from cotyledon, hypocotyls and root tip were cultured in MS medium supplemented with different concentrations of Kn, BAP and/or NAA for shoot induction. Regeneration was achieved only from cotyledon calli at a frequency of 62.50% on 5 mgL-1) BAP and 0.05 mgL-1) NAA. Shoot tip cultured for direct regeneration in the same media containing 5 mgL-1) BAP and 0.05 mgL-1) NAA resulted in 90.0% shoot differentiation in BINA mung 7. The regenerated shoots rooted on MS medium with 0.2 mgL-1) NAA (90.0).DOI: http://dx.doi.org/10.3329/pa.v19i2.16908 Progress. Agric. 19(2): 13 - 19, 2008

scholarly journals Callus Induction and Plantlet Regeneration in Blackgram (Vigna mungo L. Hepper)

2013 ◽  
Vol 19 (2) ◽  
pp. 27-35 ◽  
Author(s):  
SA Mony ◽  
MS Haque ◽  
MA Karim ◽  
SK Roy
Keyword(s):  
Shoot Regeneration ◽  
Callus Induction ◽  
Callus Formation ◽  
Plantlet Regeneration ◽  
Shoot Tip ◽  
Root Tip ◽  
Ms Medium ◽  
Plantlet Formation ◽  
Rooting Medium ◽  
Shoot Tip Explants

The present study was undertaken for callus induction and subsequent plantlet regeneration in blackgram. The study comprised of experiments for callus initiation, shoot regeneration and plantlet formation from cotyledon, hypocotyl, root tip and shoot tip explants. The effects of explants and different concentrations and combinations of BAP (0.0, 1.0, 2.5, 5.0 and 10 mg L-1) and NAA (0.0, 0.5, 1.0, 1.5 and 2.0 mg L-1)) on callus induction were investigated first. Among the explants, hypocotyls showed the best performance in callus formation (92.33%) when cultured on MS medium supplemented with 2.5 mg L-1) BAP and 1.5 mg L-1) NAA followed by cotyledon, shoot tip and root tip explants, respectively. The height percentage of shoot regeneration from the calli derived from hypocotyls (56.33%) was achieved in MS medium supplemented with 3.0 mg L-1) BAP and 0.3 mg L-1) NAA and 0.5 mg L-1) GA3. Calli from other explants had no shoot regeneration. The regenerated shoots were transferred to rooting medium supplemented with different concentrations of IBA and NAA. The high frequency (100 %) of rooting was observed with MS medium supplemented with 0.5 mg L-1) IBA. The rooted plants were transferred to pots for hardening.DOI: http://dx.doi.org/10.3329/pa.v19i2.16917 Progress. Agric. 19(2): 27 - 35, 2008

scholarly journals PROPAGATION STRATEGIES OF TRIBULUS TERRESTRIS L. A PORTENTOUS MEDICINAL PLANT EMPLOYING TISSUE CULTURE TECHNOLOGY

2017 ◽  
Vol 4 (2) ◽  
pp. 39-46
Author(s):  
Jamuna S ◽  
Anjali B ◽  
Karthika K
Keyword(s):  
Tissue Culture ◽  
Callus Induction ◽  
Root Elongation ◽  
Nodal Segments ◽  
Tribulus Terrestris ◽  
Ms Medium ◽  
Shoot Induction ◽  
Regenerated Plantlets ◽  
Juvenile Explants ◽  
Culture Technology

A protocol for micropropagation of Tribulus terrestris, an important medicinal herb was established using juvenile explants viz., leaf, node and internode. All the explants were tested for callus induction on Murashige and Skoog’s (MS) medium, supplemented with BAP, NAA and 2,4-D. Among the three explants leaf explant responded well (98%) for the callus induction in the MS medium composted with BAP and NAA (4.0 and 0.5 mg/L) followed by the nodal segments (58.75%) in the same medium. Maximum number of shoot induction from the callus of leaf derived explants (91.1%) was perceived on MS medium fortified with BAP 4.0 mg/L and NAA (0.5 mg/L). Moreover, root elongation and profuse rooting percentage (77.19%) were achieved when the well-grown shoots were cultured on MS media supplemented with IAA (2.0 mg/L) for leaf callus derived shoots. The regenerated plantlets were hardened and established at 80% survival rate in hardening media encompassed with red soil, sand and vermicompost in the ratio of 1:1:1 by volume.

scholarly journals In vitro regeneration performance of Corchorus olitorius

1970 ◽  
Vol 8 (1) ◽  
pp. 1-6 ◽  
Author(s):  
M Hoque ◽  
KM Nasiruddin ◽  
GKMN Haque ◽  
GC Biswas
Keyword(s):  
Shoot Regeneration ◽  
Callus Induction ◽  
Root Formation ◽  
Plantlet Regeneration ◽  
Ms Medium ◽  
Corchorus Olitorius ◽  
The Media ◽  
Regenerated Plantlets

The experiment was conducted during May to December 2008 in the Biotechnology Laboratory of Bangladesh Agricultural University, Mymensingh to observe the callus induction, regeneration potentiality and to establish a suitable in vitro plantlet regeneration protocol of Corchorus olitorius. MS medium supplemented with different phytohormone concentrations and combinations were used to observe the callus induction, shoot regeneration and root formation ability of the cotyledon with attached petiole derived explant of three genotypes viz. O-9897, O-72 and OM-1. The highest callus induction (92.85%) was observed in O-9897 followed by O-72 (82.14%) in the MS media supplemented with 2.5 mg/L BAP + 0.5 mg/L IAA. Genotype O-9897 in MS media supplemented with 2.5 mg/L BAP + 0.5 mg/L IAA produced the highest percentage of shoot regenerants (83.33%) followed by O-72 (75.00%) in the media supplemented with 2.5 mg/L BAP + 0.5 mg/L IAA. The root formation from regenerants was the best on halfstrength of MS media supplemented with 0.6 mg/L IBA in genotype O-9897 (45.00%). The in vitro regenerated plantlets from the genotypes O-9897 could be established in the field. Therefore, the genotypes O-9897 of C. olitorius in MS media supplemented with 2.5 mg/L BAP + 0.5 mg/L IAA could be used for callus induction and shoot regeneration. Keywords: Regeneration; Phytohormone; Corchorus olitorius DOI: 10.3329/jbau.v8i1.6390J. Bangladesh Agril. Univ. 8(1): 1-6, 2010

scholarly journals Haploid plantlet regeneration through anther culture in oilseed Brassica species

1970 ◽  
Vol 34 (4) ◽  
pp. 693-703 ◽  
Author(s):  
MA Alam ◽  
MA Haque ◽  
MR Hossain ◽  
SC Sarker ◽  
R Afroz
Keyword(s):  
Plant Regeneration ◽  
Anther Culture ◽  
Growth Regulators ◽  
Callus Induction ◽  
Brassica Species ◽  
Plantlet Regeneration ◽  
Root Induction ◽  
Ms Medium ◽  
Half Strength

Anther of five varieties of Brassica species, namely BARI Shariaha-7, Tori-7, Agrani, Daulat and Safal were cultured in vitro to observe their regeneration potentiality. Different concentrations and combinations of growth regulators were supplemented in MS medium. The range of callus induction was 12.50-87.50 %. Maximum callus induction (75.00%) was observed on MS +4 mg/L 2, 4-D + 1.0 mg/L BAP. Among the genotypes, BARI Sharisha-7 showed the highest percentage of callus induction (60.42%). Among the treatments, highest percentage of shoot regeneration (75.00%) was observed on MS + 4 mg/L BAP + 1.0 mg/L NAA. BARI Sharisha-7 also showed the highest rate of plant regeneration (66.67%). Root induction was highest (75%) on half strength MS medium supplemented with 1.0 mg/L IBA and 0.5 mg/L NAA. The plantlets with sufficient roots thus obtained were transferred successfully to plastic pots and subsequently to the field. BARI Sharisha-7 and Tori-7 survived easily in the pots as well as in the field but Safal was very poor in survivability both in the pots and in the field. Key Words: Brassica; haploid; anther culture; in vitro regeneration.DOI: 10.3329/bjar.v34i4.5844Bangladesh J. Agril. Res. 34(4) : 693-703, December 2009 

Cytokinin-cytokinin interaction ameliorates the callus induction and plant regeneration of tomato (Solanum lycopersicum Mill.)

2012 ◽  
Vol 60 (1) ◽  
pp. 47-55 ◽  
Author(s):  
A. Ali ◽  
T. Yossef ◽  
A. El-Banna
Keyword(s):  
Plant Regeneration ◽  
Solanum Lycopersicum ◽  
Callus Induction ◽  
Ms Medium ◽  
Regeneration System ◽  
Benzyl Adenine ◽  
Cotyledon Explants ◽  
Tomato Genotypes ◽  
Number Of Shoots

The present study was carried out for developing an efficient in vitro callus induction and plant regeneration system in four different tomato genotypes (Solanum lycopersicum Mill., previous name: Lycopersicon esculentum), Advantage II, Edkawy, Castle Rock and Super Strain B, using hypocotyl and cotyledon explants. The effects of two cytokinins, BA (benzyl adenine) and Kin (kinetin), on callus induction and plant regeneration frequency were investigated when added to MS medium in combination at varying concentrations. All concentrations of the two cytokinins were suitable for callus induction and plant regeneration. The frequency of callus induction and plant regeneration from both cotyledon and hypocotyl explants reached 100% for all tested genotypes. Cotyledons produced a higher average number of shoots per explants than hypocotyls for all the genotypes in the five concentrations of combined cytokinins. The average number of shoots per explant in Super Strain B was found to be the highest (42 and 60 for the hypocotyl and cotyledon explants, respectively). Supplementing MS medium with 1.0 mg L−1 kinetin and 1.0 mg L−1 benzyl adenine was found to be optimum for producing the highest number of shoots per explant from hypocotyls and cotyledons in the tomato genotypes investigated. The proposed medium showed a significant superiority over the reference media.

scholarly journals Callus Induction and Plantlet Regeneration From Centalla Asiatica Linn

2004 ◽  
Vol 2 (2) ◽  
pp. 81-85
Author(s):  
Indumathy A. ◽  
Mahalakshmi P. ◽  
C. R. Bojan
Keyword(s):  
Plant Regeneration ◽  
Callus Induction ◽  
Leaf Explants ◽  
Centella Asiatica ◽  
Plantlet Regeneration ◽  
Nodal Segments ◽  
Ms Medium ◽  
Root Proliferation ◽  
Mass Propagation

Efficient plant regeneration could be obtained from the derooted nodal segments of Centella asiatica with stole buds as the explant, when cultured on MS medium supplemented with BAP (10 ppm)+NAA(2ppm). Both callus and regeneration occurred simultaneously on the same medium. Profuse rooting were obtain on MS medium fortified with NAA (2ppm) from leaf explants. Shoot and root proliferation were observed on the medium supplemented with BAP(5 ppm)and NAA (2 ppm) through subculture. Mass propagation of plantlets were obtained through invitro culture.

scholarly journals Effect of plant growth regulators and explant types on induction and growth of callus of Primula veris L.

2013 ◽  
Vol 59 (3) ◽  
pp. 17-25
Author(s):  
Maria Morozowska ◽  
Maria Wesołowska ◽  
Romana Głowicka-Wołoszyn ◽  
Anna Kosińska
Keyword(s):  
Callus Induction ◽  
Suitable Condition ◽  
Ms Medium ◽  
Light Conditions ◽  
Root Explants ◽  
Cotyledon Explants ◽  
Primula Veris ◽  
Callus Initiation ◽  
Callus Proliferation ◽  
Time Required

Abstract Primula veris L. (Primulaceae) is a well-known medicinal herb. The callus induction response of three explant types: roots, cotyledons, and hypocotyls of four-week-old cowslip seedlings were evaluated. The highest statistically different callus induction rate was 93.6% and was obtained from root explants on MS medium supplemented with 0.1 mg/l BA and 5.0 mg/l PIC. Calli also appeared on 83.3% of cotyledon explants on MS medium supplemented with 1.0 mg/l BA and 3.5 mg/l 2,4-D and on 81.0% of root explants on MS medium supplemented with 0.1 mg/l KIN and 2.0 mg/l 2,4-D. These values were not statistically different. The average time required for callus initiation was 4 to 6 weeks, however, it depended on the explants type. The most suitable condition for callus proliferation and growth was MS medium with 0.5 mg/l TDZ and 0.1 mg/l NAA, and with 1.0 mg/l BA and 2.0 mg/l or 3.5 mg/l 2,4-D. No light conditions proved to be more favourable for cowslip calli induction and growth

Production of Glycyrrhizin in Callus Cultures of Licorice

2008 ◽  
Vol 63 (5-6) ◽  
pp. 413-417 ◽  
Author(s):  
Winida Wongwicha ◽  
Hiroyuki Tanaka ◽  
Yukihiro Shoyama ◽  
Indree Tuvshintogtokh ◽  
Waraporn Putalun
Keyword(s):  
Callus Induction ◽  
Shoot Formation ◽  
Callus Cultures ◽  
Glycyrrhiza Glabra ◽  
Naphthaleneacetic Acid ◽  
Stem Explants ◽  
Ms Medium ◽  
Shoot Induction

Licorice plants, Glycyrrhiza glabra, G. uralensis, and G. inflata, were investigated for callus induction using Murashige and Skoog (MS) medium combined with auxins and cytokinins. After 4 weeks of culture, 33-100% of leaf or stem explants formed calli. Maximum of shoot induction from callus cultures was achieved by G. inflata stem explants cultured on MS medium supplemented with 1 mg/l α-naphthaleneacetic acid (NAA) and 0.5 mg/l 6-benzyladenine (BA) (67%) which also gave maximum shoot formation per explant (two shoots per explant). These results indicated that all three Glycyrrhiza species regenerated shoots from callus cultures on MS medium combined with NAA and BA or only thidiazuron (TDZ; 0.1 and 0.5 mg/l). Glycyrrhizin contents of G. uralensis calli induced using MS medium in combination with NAA and BA [(27.60 ± 8.47) μg/g DW] or TDZ alone [(36.52 ± 2.45) μg/ g DW] were higher than those found in other combinations.

Production of Isoflavonoids in Callus Cultures of Pueraria candollei var. mirifica

2009 ◽  
Vol 64 (3-4) ◽  
pp. 239-243 ◽  
Author(s):  
Latiporn Udomsuk ◽  
Kanokwan Jarukamjorn ◽  
Hiroyuki Tanaka ◽  
Waraporn Putalun
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Callus Induction ◽  
Culture Medium ◽  
Callus Cultures ◽  
Stem Explants ◽  
Ms Medium ◽  
Shoot Induction ◽  
Pueraria Candollei

Pueraria candollei Wall. ex Benth. var. mirifica (Airy Shaw & Suvat.) Niyomdham was investigated for callus induction using Murashige and Skoog (MS) medium containing different plant growth regulators. After 8 weeks of culture, 66 - 100% of leaf or stem explants formed calli. Calli from stem explants cultured on MS medium supplemented with 0.5 mg/l thidiazuron (TDZ) gave the maximum of shoot induction (16%) and the highest level of total isoflavonoids [(50.39 ± 7.06) mg/g dry wt], which was 7-fold higher than that of the native tuber [(7.04 ± 0.29) mg/g dry wt]. These results suggest that addition of TDZ to the culture medium markedly enhances the production of isoflavonoids in calli induced from stem explants of P. candollei var. mirifica.

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