scholarly journals In vitro Micropropagation of Abutilon indicum L. through Leaf Explants

1970 ◽  
Vol 19 (2) ◽  
pp. 177-184 ◽  
Author(s):  
Jyoti Ranjan Rout ◽  
Manorama Mishra ◽  
Ritarani Das ◽  
Santi Lata Sahoo
Keyword(s):  
Callus Induction ◽  
Leaf Explants ◽  
Shoot Formation ◽  
Plantlet Regeneration ◽  
Root Induction ◽  
Ex Vitro ◽  
In Vitro Micropropagation ◽  
Half Strength ◽  
Abutilon Indicum

The present investigation was conducted to develop a protocol for rapid callus induction and plant regeneration from leaf explant of Abutilon indicum L. Callus induction and plantlet regeneration at various frequencies were observed on MS using different concentrations of 2,4-D alone or in combination with BAP and Kn. The highest percentage of callus induction was observed with 2.5 mg/l 2,4-D (90) and with combination of 0.5 mg/l Kn (85). Optimum shoot formation was observed on same medium but supplemented with 2.0 mg/l Kn and 1.0 mg/l NAA (11.2). Rooting experiments with half strength of MS revealed that NAA was more suitable for root induction compared to IBA and IAA. The healthy in vitro rooting plantlets were successfully transferred to the field. The survival of the plantlets under ex vitro condition was 87%. Key words: Abutilon indicum, Callus induction, Leaf explants, Micropropagation D.O.I. 10.3329/ptcb.v19i2.5435 Plant Tissue Cult. & Biotech. 19(2): 177-184, 2009 (December)

scholarly journals IN VITRO REGENERATION AND MASS PROPAGATION OF AZIMA TETRACANTHA [LAM.] FROM THE LEAF EXPLANTS THROUGH CALLUS CULTURE

2017 ◽  
Vol 4 (2) ◽  
pp. 52-56
Author(s):  
Mallika Devi T
Keyword(s):  
Callus Induction ◽  
In Vitro Regeneration ◽  
Leaf Explants ◽  
Shoot Formation ◽  
Ms Medium ◽  
Apical Leaf ◽  
Half Strength ◽  
Regenerated Plantlets ◽  
Azima Tetracantha

In the present study the protocol for callus induction and regeneration in Azima tetracantha has been developed in culture medium. The young apical leaf explants were used for callus induction on MS medium containing BAP and NAA at 1.0 and 0.4mgl-1 respectively showed maximum callus induction (73%). The amount of callus responded for shoot formation (74%) was obtained in the MS medium containing BAP (1.5 mgl-1) and NAA (0.3mgl-1).The elongated shoots were rooted on half strength medium supplemented with IBA (1.5 mgl-1) and Kn (0.4 mgl-1) for shoots rooted. Regenerated plantlets were successfully acclimatized and hardened off inside the culture and then transferred to green house with better survival rate.

scholarly journals Haploid plantlet regeneration through anther culture in oilseed Brassica species

1970 ◽  
Vol 34 (4) ◽  
pp. 693-703 ◽  
Author(s):  
MA Alam ◽  
MA Haque ◽  
MR Hossain ◽  
SC Sarker ◽  
R Afroz
Keyword(s):  
Plant Regeneration ◽  
Anther Culture ◽  
Growth Regulators ◽  
Callus Induction ◽  
Brassica Species ◽  
Plantlet Regeneration ◽  
Root Induction ◽  
Ms Medium ◽  
Half Strength

Anther of five varieties of Brassica species, namely BARI Shariaha-7, Tori-7, Agrani, Daulat and Safal were cultured in vitro to observe their regeneration potentiality. Different concentrations and combinations of growth regulators were supplemented in MS medium. The range of callus induction was 12.50-87.50 %. Maximum callus induction (75.00%) was observed on MS +4 mg/L 2, 4-D + 1.0 mg/L BAP. Among the genotypes, BARI Sharisha-7 showed the highest percentage of callus induction (60.42%). Among the treatments, highest percentage of shoot regeneration (75.00%) was observed on MS + 4 mg/L BAP + 1.0 mg/L NAA. BARI Sharisha-7 also showed the highest rate of plant regeneration (66.67%). Root induction was highest (75%) on half strength MS medium supplemented with 1.0 mg/L IBA and 0.5 mg/L NAA. The plantlets with sufficient roots thus obtained were transferred successfully to plastic pots and subsequently to the field. BARI Sharisha-7 and Tori-7 survived easily in the pots as well as in the field but Safal was very poor in survivability both in the pots and in the field. Key Words: Brassica; haploid; anther culture; in vitro regeneration.DOI: 10.3329/bjar.v34i4.5844Bangladesh J. Agril. Res. 34(4) : 693-703, December 2009 

Notes on In-vitro Propagation of Tecomella undulata (Sm.) Seem

2017 ◽  
Vol 24 (4) ◽  
pp. 217-220
Author(s):  
Rakesh Poria ◽  
K. Bangarwa ◽  
Kavita Sharma ◽  
R. Yadav
Keyword(s):  
In Vitro Propagation ◽  
Dark Period ◽  
Leaf Explants ◽  
Multiple Shoots ◽  
Shoot Formation ◽  
Root Induction ◽  
Step Procedure ◽  
Half Strength ◽  
Tecomella Undulata

Different explants of Tecomella undulata (Sm.) Seem. cultured on modified MS media gave significant differences for shoot regeneration and root differentiation. Plantlets were successfully developed using axillary bud and cotyledon as explants. There was no shoot formation from leaf explants. Maximum multiple shoots from axillary buds and cotyledons were obtained on medium containing MS major salts with BAP 4.0 mg/l + IAA 0.1 mg/l and BAP 5.0 mg/l + NAA 0.5 mg/l, respectively. Healthy roots developed from two step procedure. Isolated shoots were rooted by culturing on half strength MS liquid medium having IBA 2.5 mg/l for 48 hrs and then transferring to hormone free half strength solid MS medium. An initial dark period for 5-7 days favoured root induction. About 55 percent of in-vitro produced shoots were rooted.

scholarly journals CHIA SEED AS A SOURCE OF IN VITRO ESTABLISHMENT OF Salvia hispanica L. PLANTS

2020 ◽  
Vol 51 (3) ◽  
pp. 976-981
Author(s):  
Al- Dabagh & Salih
Keyword(s):  
Shoot Multiplication ◽  
Shoot Formation ◽  
Root Induction ◽  
Ms Medium ◽  
Salvia Hispanica ◽  
Chia Seed ◽  
Half Strength ◽  
Salvia Hispanica L ◽  
The Impact

 Technique of tissue culture for Chia (Salvia hispanica) micropropagation was achieved, this study investigated the impact of various concentrations of plant growth regulators on shoot multiplication and root induction with the Chia’s mature seed as a source explant. The highest percentage of shoot formation (80%), shoots number per explant(3.20) and shoot length(3.26 cm), were recorded on MS medium enriched with BAP(1.0 mgl-1) after eight weeks of seed culture. The optimal medium for the rhizogenesis was achieved on half strength MS medium fortified with 1.0 mgl-1 IBA after four weeks of culture, which had the highest rooting percentage (100%) with highest mean of roots number (5.6 roots per shoot) with (3.40 cm root length). The rooted plants were successfully adapted ex vitro with a survival rate of 85%.

scholarly journals Optimizing the concentrations of plant growth regulators for in vitro shoot cultures, callus induction and shoot regeneration from calluses of grapes

OENO One ◽  
2015 ◽  
Vol 49 (1) ◽  
pp. 37 ◽  
Author(s):  
Nadra Khan ◽  
Maqsood Ahmed ◽  
Ishfaq Hafiz ◽  
Nadeem Abbasi ◽  
Shaghef Ejaz ◽  
...  
Keyword(s):  
Shoot Regeneration ◽  
Growth Regulators ◽  
Callus Induction ◽  
Leaf Explants ◽  
Shoot Cultures ◽  
Ms Medium ◽  
Grape Cultivar ◽  
Half Strength ◽  
Number Of Shoots

<p style="text-align: justify;"><strong>Aim</strong>: To optimize the concentrations of growth regulators in the media for the proficient micropropagation of grapevine (<em>Vitis vinifera </em>L.) cv. King’s Ruby.</p><p style="text-align: justify;"><strong>Methods and results</strong>: Apical meristems of the grape cultivar were used to establish <em>in vitro</em> shoot cultures. Nodal explants, each containing an axillary bud, taken from <em>in vitro</em> grown shoots were inoculated in shoot proliferation medium, i.e., half strength Murashige and Skoog (MS) medium supplemented with benzyl aminopurine (BAP), kinetin, glycine and gibberellic acid (GA<sub>3</sub>). A higher number of shoots (5.33) with greater shoot length (2.75 cm) was produced in the medium supplemented with 1.0 mg L<sup>-1</sup> BAP and 0.1 mg L<sup>-1</sup> GA<sub>3</sub>. Calluses were induced from leaf explants taken from <em>in vitro</em> grown shoots. Callus induction was greater (73.00%) on the medium containing 2.0 mg L<sup>-1</sup> 2,4-dichlorophenoxyacetic acid (2,4-D), 0.3 mg L<sup>-1</sup> BAP and 0.2 mg L<sup>-1</sup> α-naphthaleneacetic acid (NAA). The maximum frequency of shoot regeneration (53.33%) was achieved on the medium supplemented with 1.5 mg L<sup>-1</sup> BAP and 0.5 mg L<sup>-1</sup> NAA, and the regenerated shoots successfully formed roots on growth regulator-free half strength MS medium.</p><p style="text-align: justify;"><strong>Conclusion</strong>: Optimizing the concentration of BAP and GA<sub>3</sub> and omitting the glycine and kinetin in the culture medium increased the number and length of shoots. Similarly, for inducing the callus of the leaf explants, taken from <em>in vitro</em> grown shoots, it is recommended to adjust the medium with the higher concentration of 2,4-D and lower concentrations of BAP. Moreover, the maximum number of shoots was regenerated on a medium supplemented with relatively high levels of both BAP and NAA (1.5 and 0.5 mg L<sup>-1</sup>, respectively). Finally, we suggest the half strength MS medium that is free from growth regulators for the root formation of the regenerated shoots.</p><p style="text-align: justify;"><strong>Significance and impact of the study</strong>: Optimizing the concentration of growth regulators is crucial for the efficient micropropagation of a grape cultivar. Knowing the specific balance between the growth regulators is necessary to establish <em>in vitro</em> shoot cultures, callus induction and shoot regeneration and, hence, to propagate disease-free true to type grape cultivars in a short time.</p>

scholarly journals IN VITRO DIRECT MULTIPLE SHOOT INDUCTION FROM LEAF EXPLANTS OF SOLANUM PUBESCENS WILLD

2016 ◽  
Vol 4 (12SE) ◽  
pp. 23-28
Author(s):  
Ayyadurai V ◽  
Ramar K
Keyword(s):  
Leaf Explants ◽  
Multiple Shoots ◽  
Multiple Shoot ◽  
Root Induction ◽  
Ms Medium ◽  
Multiple Shoot Induction ◽  
Half Strength ◽  
Multiple Shoot Regeneration ◽  
Solanum Pubescens

Efficientin Vitro direct multiple shoot regeneration from Solanum pubescens was achieved from leaf explants on MS medium Sublimated with B5 vitamins and different concentrations and different combinations of PGRs like BAP, NAA and GA3. The maximum numbers of multiple shoots were achieved from leaf explants on 3.0 mg/l BAP + 1.0mg/l GA3. The regenerated shoots were transferred in to half strength MS medium fortified with IBA for root induction. Rooted plantlets were successfully acclimatized. This new and transfer into the field Conditions. Standardized and reproducible protocol useful the mass propagation of Solanum pubescens.

scholarly journals In vitro clonal propagation, organogenesis and somatic embryogenesis in Bacopa monnieri (L.) Wettst

2019 ◽  
Vol 6 (4) ◽  
pp. 442-449
Author(s):  
Dipu Samanta ◽  
Bidisha Mallick ◽  
Debleena Roy
Keyword(s):  
Somatic Embryogenesis ◽  
Callus Induction ◽  
Clonal Propagation ◽  
Low Cost ◽  
Leaf Explants ◽  
Shoot Formation ◽  
Bacopa Monnieri ◽  
In Vitro Clonal Propagation

Bacopa monnieri (L.) Wettst is a well-known medicinal herb in the Ayurveda. It is also used as laxative and curative for ulcers, inflammation, anaemia, scabies, leucoderma, asthma and epilepsy, enlargement of spleen, leprosy and others. In vitro propagation and regeneration through somatic embryogenesis of B. monnieri has played an important role in the production of healthy, disease-free plants with desirable traits. In B. monnieri, there are few reports which indicate rapid regeneration and somatic embryogenesis. For in vitro clonal propagation, the highest shoot formation was obtained when BAP 2 mg/ l used. The best response for rooting was obtained in IAA 1.0 mg/ l. The recorded survival rate of the plants was 70%. Plants were without any detectable phenotypic variations. Cytological study indicated that the chromosome number remain same (2n= 64) in in vitro and in vivo roots. A rapid, simple and efficient protocol for plantlet regeneration was achieved through embryogenic callus from leaf explants of B. monnieri. Callus induction and embryogenesis were significantly affected by presence/absence and type and concentration of growth regulators. Best organogenic callus induction was obtained in MS medium supplemented with BAP 5mg/ l. For induction of somatic embryogenesis, auxin (2, 4-D 1 mg/ l) was used in the culture medium subsequently in basal media for embryo maturation. Kn 0.2 mg/ l was the best for production of plantlet from embryo. Thus, this can be an easiest protocol for stable clonal propagation and plant regeneration through somatic embryogenesis in B. monnieri. The protocol used here for propagation and regeneration is much easier, low cost and reliable.

scholarly journals Enhanced Regeneration Through ex vitro Rooting and Agrobacterium-mediated Genetic Transformation of Eggplant (Solanum melongena L.)

2021 ◽  
Vol 31 (1) ◽  
pp. 97-108
Author(s):  
Sabina Yesmin ◽  
MI Hoque ◽  
RH Sarker
Keyword(s):  
Genetic Transformation ◽  
Solanum Melongena ◽  
Leaf Explants ◽  
Ex Vitro Rooting ◽  
Bacterial Suspension ◽  
Root Induction ◽  
Ex Vitro ◽  
Regeneration System ◽  
Cotyledonary Leaf

Regeneration of in vitro multiple shoots was achieved through organogenesis on MS supplemented with 2.0 mg/l BAP and 0.5 mg/l Kn from cotyledonary leaf explants of two local varieties of eggplant (Solanum melongena L.). Elongation of regenerated shoots was obtained on growth regulator free MS. In vitro root induction from excised regenerated shoots was less effective on MS with or without plant growth regulators. On the other hand regenerated shoots treated with 10 mM IBA for 5 min were found to be effective for ex vitro rooting in sterilized soil. Following sufficient development of roots, the ex vitro rooted plantlets were acclimatized in growth room condition, and were transferred to the field having 100% survival rate. The regeneration system developed was utilized for Agrobacterium-mediated genetic transformation using Agrobacterium tumefaciens strain LBA4404/pBI121 containing GUS and nptII genes. Adequate transformation response was obtained from cotyledonary leaf segments with bacterial suspension having an optical density of 0.50 at 600 nm with 30 min incubation followed by co-cultivation period of 72 hrs in Nayantara (BARI Begun-5) variety of eggplant. Selection of transformed shoots was carried out on MS supplemented with 2.0 mg/l BAP, 0.5 mg/l Kn, 300 mg/l carbenicillin and 100 mg/l kanamycin. Stable integration of GUS and nptII genes in Nayantara were confirmed through PCR analysis using the genomic DNA isolated from transformed shoots. Plant Tissue Cult. & Biotech. 31(1): 97-108, 2021 (June)

scholarly journals In vitro Seed Germination, Protocorm Formation and Plantlet Regeneration in Aerides ringens Fisher

2019 ◽  
Vol 29 (1) ◽  
pp. 49-62 ◽  
Author(s):  
Ashok N Pyati
Keyword(s):  
Survival Rate ◽  
Seed Germination ◽  
Plantlet Regeneration ◽  
Ex Vitro ◽  
Coconut Husk ◽  
Half Strength ◽  
The Media ◽  
Basal Media ◽  
Protocorm Formation

An attempt was made to in vitro seed germination and plantlet regeneration in Aerides ringens, a threatened, endemic and epiphytic orchid. Five basal media Knudson C (KC), Vacin and Went (VW), Burgeff’s (N3f), half strength MS, MS were examined for their effectiveness. Germination occurred in all the media. However, KC medium proved to be the best for seed germination (70.8%) and half strength MS was superior for the differentiation of protocorm like bodies (PLBs) into seedlings. The seeds were also cultured on various plant growth regulators (PGRs) to evaluate their effectiveness on seed germination and further differentiation. Among the PGRs tested, 0.57 μM IAA (IAA 89.3%), 4.64 μM Kn (88.6%) and 2.21 μM BAP (90.7%) and 0.57 μM IAA + 0.55 μM BAP (96.5%) were stimulated the germination and further differentiation of PLBs into seedlings. The concentrations of 2.21 μM BAP and combination of 0.57 μM IAA + 0.55 μM BAP stimulated the formation of MPLBs, which later differentiated into shoots and roots. Four potting substrates were evaluated for Ex vitro seedling survivability of which brick: charcoal: decaying litter: coconut husk (1 : 1 : 1 : 1) gave maximum survival rate (89.0%). Plant Tissue Cult. & Biotech. 29(1): 49-62, 2019 (June)

scholarly journals In vitro regeneration of popular tobacco varieties of Bangladesh from leaf disc

1970 ◽  
Vol 35 (1) ◽  
pp. 125-134 ◽  
Author(s):  
MA Rahman ◽  
MA Alam ◽  
MR Hossain ◽  
A Hossain ◽  
R Afroz
Keyword(s):  
Callus Induction ◽  
Callus Formation ◽  
In Vitro Regeneration ◽  
Leaf Disc ◽  
Shoot Formation ◽  
Plantlet Regeneration ◽  
Regeneration Ability ◽  
Ms Medium ◽  
Leaf Discs

Regeneration ability of five Nicotiana varieties viz., Virginia, Jati, Motihari, CC Bengal and Sumatra were investigated via callus induction using leaf discs. Explants were cultured on MS medium supplemented with different concentrations and combinations of plant growth regulators. Callus formation frequency was 67.20%. Among the varieties used, Motihari induced the highest percentage (97.50%) of callus followed by Jati (92.50%) in 2.0 rng/L Kinetin and 2.0 mg/L IAA. Shoots were induced from calli cultured on the same medium. Maximum shoot formation from leaf discs was 82.50% on medium supplemented with 2.0 mg/L Kinetin and 2.0 mg/L IAA. It was also revealed from this study that Motihari was the best variety for callus formation and subsequent plantlet regeneration which is a pre-requisite for vector mediated transformation for varietal improvement of Nicotiana species. The rooting response of regenerated shoots was observed by using ½ MS medium with IBA (0.0, 0.5, and 1.0 mg/L). The highest root formation was found in Motihari (90%) with ½ MS medium supplemented with 0.5 mg/L IBA. After that regenerated plantlets with plenty of roots were transferred successfully to pots and subsequently to the field. Keywords: Tobacco; Nicotiana; in vitro regeneration; callus induction; plantlet regeneration; leaf disc; phytohormone. DOI: 10.3329/bjar.v35i1.5873Bangladesh J. Agril. Res. 35(1) : 125-134, March 2010

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