scholarly journals Induction of Direct Adventitious Shoot Regeneration in Pear (Pyrus communis L.)

2014 ◽  
Vol 24 (1) ◽  
pp. 87-92 ◽  
Author(s):  
Mahdieh Yousefiara ◽  
Maryam Jafarkhani Kermani ◽  
Abdolreza Bagheri ◽  
Ali Akbar Habashi ◽  
Hamid Abdollahi
Keyword(s):  
Shoot Regeneration ◽  
Culture Media ◽  
Adventitious Shoot ◽  
Pyrus Communis ◽  
Explant Type ◽  
Direct Shoot Regeneration ◽  
Adventitious Shoot Regeneration ◽  
Engineering Research ◽  
Pyrus Communis L ◽  
Direct Shoot

Efficient direct shoot regeneration of two pear (Pyrus communis L.), namely  'bartlett' and 'dargazi' was successfully developed for the use in future genetic engineering research. The basal MS and NN media supplemented with different concentrations of TDZ (0, 2.5, 5, 7.5 ?M) or BAP (0, 4, 8, 16 ?M) in combination with NAA (1 ?M) were compared. The result showed that direct adventitious shoot regeneration in pear was highly dependent on genotype, explant type and culture media. 'dargazi' had higher rate of regeneration compared to 'bartlett' and in both cultivars the highest per cent of regeneration was observed in lower sections of the leaves. Although the highest per cent of regeneration in 'bartlett' (38) was attained in the NN medium containing 2.5 µM TDZ and 1 µM NAA, but the differences in shoot regeneration between media containing 5 or 7.5 µM TDZ and 1 µM NAA were not significant. The highest per cent of regeneration in 'dargazi' (56) was obtained in NN medium containing 7.5 µm TDZ and 1 µm NAA. Plant Tissue Cult. & Biotech. 24(1): 87-92, 2014 (June) D. O. I. http://dx.doi.org/10.3329/ptcb.v24i1.19215

scholarly journals Tissue culture of Cecropia glaziovii Sneth (urticaceae): vegetative micropropagation and plant regeneration from callus

2010 ◽  
Vol 34 (5) ◽  
pp. 1245-1252 ◽  
Author(s):  
Marcos Nopper Alves
Keyword(s):  
Tissue Culture ◽  
Acetic Acid ◽  
Shoot Regeneration ◽  
Culture Media ◽  
Adventitious Shoot ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Adventitious Shoot Regeneration ◽  
Popular Medicine ◽  
Superior Genotype

Cecropia glaziovii is a tree with used in Brazilian popular medicine. Methods allowing the clonal propagation of this species are of great interest for superior genotype multiplication and perpetuation. For this reason, we examined the effect of different culture media and different types of explants on adventitious shoot regeneration from callus and buds of C. glaziovii. Leaves, petioles and stipules obtained from aseptically grown seedlings or from pre-sterilized plants were used to initiate cultures. Adventitious shoot regeneration was achieved when apical and axillary buds were inoculated on gelled Murashige & Skoog (MS) medium supplemented with 6-benzylaminopurine alone (BAP) (1.0, 5.0 or 10.0 mg L-1) or combined with -naphthalene acetic acid (NAA) (1.0 or 2.0 mg L-1), after 40 days of culture. Best callus production was obtained after 30 days of petioles' culture on gelled MS medium with 2,4 dichlorophenoxyacetic acid (2,4-D) (5.0 mg L-1) combined with BAP (1.0 mg L-1). Successful shoot regeneration from callus was achieved when MS medium supplemented with zeatin (ZEA) (0.1 mg L-1) alone or combined with 2,4-D (1.0 or 5.0 mg L-1) was inoculated with friable callus obtained from petioles. All shoots were rooted by inoculation on MS medium supplemented with indole-3-acetic acid (IAA) (1.0 mg L-1). Rooted plants transferred to potting soil were successfully established. All in vitro regenerated plantlets showed to be normal, without morphological variations, being also identical to the source plant. Our study has shown that C. glaziovii can be propagated by tissue culture methods, allowing large scale multiplication of superior plants for pharmacological purposes.

Direct shoot regeneration from epicotyl explants of grasspea (Lathyrus sativus)

2006 ◽  
Vol 54 (5) ◽  
pp. 505 ◽  
Author(s):  
Durga Prasad Barik ◽  
Umaballava Mohapatra ◽  
Pradeep Kumar Chand
Keyword(s):  
Shoot Regeneration ◽  
Adventitious Shoot ◽  
Lathyrus Sativus ◽  
Grain Legume ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Direct Shoot Regeneration ◽  
Murashige Skoog ◽  
Seedling Explant ◽  
Direct Shoot

A reproducible procedure is described for adventitious shoot organogenesis in epicotyl segments resulting in prolific plant regeneration of a grain legume grasspea (Lathyrus sativus L.). Among seedling explant types examined, epicotyl segments were most responsive. The highest percentage of direct shoot regeneration was elicited on Murashige–Skoog (MS) medium augmented with 4.0 mg L–1 6-benzyladenine (BA) + 2.0 mg L–1 α-naphthaleneacetic acid (NAA). Compared with four other genotypes examined, IC-120487 showed the highest shoot regeneration frequency (approximately 80%) with maximum shoot numbers (averaging eight shoots per explant) and longest average shoot length (approximately 4 cm). Rhizogenesis was induced in ~78% of the regenerated shoots in half-strength MS medium containing 0.5 mg L–1 indole-3-acetic acid (IAA). Plantlets were acclimated in vermi-compost and 75% of those transferred to soil survived and set viable seeds.

scholarly journals Adventitious Shoot Regeneration and Agrobacterium tumefaciens-mediated Transient Transformation of Almond × Peach Hybrid Rootstock ‘Hansen 536’

HortScience ◽  
2019 ◽  
Vol 54 (5) ◽  
pp. 936-940 ◽  
Author(s):  
Xiaojuan Zong ◽  
Brandon J. Denler ◽  
Gharbia H. Danial ◽  
Yongjian Chang ◽  
Guo-qing Song
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Shoot Regeneration ◽  
Prunus Persica ◽  
Culture Media ◽  
Leaf Explants ◽  
Adventitious Shoot ◽  
Annual Rainfall ◽  
Transient Transformation ◽  
Regeneration System ◽  
Adventitious Shoot Regeneration

‘Hansen 536’ (Prunus dulcis × Prunus persica) is an important commercial rootstock for peach and almond. However, susceptibility to wet soil and bacterial canker has limited its use primarily to areas with less annual rainfall. Genetic engineering techniques offer an attractive approach to improve effectively the current problems with this cultivar. To develop an efficient shoot regeneration system from leaf explants, 10 culture media containing Murashige and Skoog (MS) or woody plant medium (WPM) supplemented with different plant growth regulators were evaluated, and adventitious shoot regeneration occurred at frequencies ranging from 0% to 36.1%. Optimal regeneration with a frequency of 32.3% to 36.1% occurred with WPM medium containing 8.88 µm 6-benzylamino-purine (BAP) and 0.98 to 3.94 µm indole-3-butyric acid (IBA). The regenerated shoots had a high rooting ability, and 80% of the in vitro shoots tested rooted and survived after being transplanted to substrate directly. Transient transformation showed an efficient delivery of the β-glucuronidase (GUS) reporter gene (gusA) using all three Agrobacterium tumefaciens strains tested with a concentration of OD600 0.5 to 1.0 for 4 days of cocultivation. The protocols described provide a foundation for further studies to improve shoot regeneration and stable transformation of the important peach and almond rootstock ‘Hansen 536’.

scholarly journals EFFECT OF GA3 AND PACLOBUTRAZOL ON ADVENTITIOUS SHOOT REGENERATION OF TWO PELARGONIUM SP.

2012 ◽  
pp. 187-194 ◽  
Author(s):  
L. Hamama ◽  
L. Voisine ◽  
A. Naouar ◽  
R. Gala ◽  
D. Cesbron ◽  
...  
Keyword(s):  
Shoot Regeneration ◽  
Adventitious Shoot ◽  
Adventitious Shoot Regeneration

scholarly journals Rejuvenation of chicory and lettuce plants following phase change in tissue culture

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Anthony J. Conner ◽  
Helen Searle ◽  
Jeanne M. E. Jacobs
Keyword(s):  
Tissue Culture ◽  
Shoot Regeneration ◽  
Seed Set ◽  
Genetic Manipulation ◽  
Adventitious Shoot ◽  
In Vitro Flowering ◽  
Phase Changes ◽  
Adventitious Shoot Regeneration ◽  
Cell And Tissue Culture

Abstract Background A frequent problem associated with the tissue culture of Compositae species such as chicory (Cichorium intybus L.) and lettuce (Lactuca sativa L.) is the premature bolting to in vitro flowering of regenerated plants. Plants exhibiting such phase changes have poor survival and poor seed set upon transfer from tissue culture to greenhouse conditions. This can result in the loss of valuable plant lines following applications of cell and tissue culture for genetic manipulation. Results This study demonstrates that chicory and lettuce plants exhibiting stable in vitro flowering can be rejuvenated by a further cycle of adventitious shoot regeneration from cauline leaves. The resulting rejuvenated plants exhibit substantially improved performance following transfer to greenhouse conditions, with increased frequency of plant survival, a doubling of the frequency of plants that flowered, and substantially increased seed production. Conclusion As soon as in vitro flowering is observed in unique highly-valued chicory and lettuce lines, a further cycle of adventitious shoot regeneration from cauline leaves should be implemented to induce rejuvenation. This re-establishes a juvenile phase accompanied by in vitro rosette formation, resulting in substantially improved survival, flowering and seed set in a greenhouse, thereby ensuring the recovery of future generations from lines genetically manipulated in cell and tissue culture.

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