scholarly journals Physiological response to the action of the agrotoxic 2,4-Dichlorophenoxiacetic acid in Saccharomyces cerevisiae

2021 ◽  
Vol 10 (11) ◽  
pp. e408101119912
Author(s):  
Débora Tavares Sarabia ◽  
Larissa Pires Mueller ◽  
Maria do Socorro Mascarenhas Santos ◽  
Margareth Batistote
Keyword(s):  
Methylene Blue ◽  
Physiological Response ◽  
Pure Water ◽  
Cellular Growth ◽  
Toxic Substances ◽  
Target Species ◽  
Phenotypic Profile ◽  
Ultra Pure Water ◽  
Toxicity Effect ◽  
Genetic Mechanisms

The presence of xenobiotic compounds in the environment is responsible for impacts on the ecosystem. An example is pesticides that pose risks to non-target species, such as microorganisms present in the soil and that are responsible for cycling nutrients, some can be used to measure the effects of these compounds, such as yeasts because when exposed to toxic substances begin to present changes in physiological and genetic mechanisms. Thus, this study aims to evaluate the toxicity effect of the 2,4-D pesticide on Pedra-2 (PE-2) and Fleischmann® (FLE) yeasts. 2,4-D dilutions of 2.0; 4.0 and 6.0 µg L-1 were added to a solution prepared with 20 ml of ultra-pure water and 2.0 g of sucrose where the yeasts were grown. The phenotypic profile of yeasts against the toxicity of the compound was evaluated quantitatively with cell viability. Using the methylene blue method and qualitatively with cell growth tests in Petri dishes containing 2% YPD medium and flocculation on slides with methylene blue. Yeasts showed loss of viability and the FLE strain showed greater sensitivity, the cellular growth of this yeast was also more affected and, consequently, presented higher flocculation rates. The data show that the longer exposure time and the doses and concentrations of 2,4-D interfered with the physiological response of the FLE yeast. Thus, we can suggest that this microorganism has the potential to be considered for environmental tests and analyses as a bioindicator.

scholarly journals Optimization of the Novel Bisulfite Steeping Method by Response Surface Methodology for Natural Starch Synthesis From Oil Palm Trunk Biomass

2020 ◽  
Author(s):  
Zaber Ahmed ◽  
Mohd Suffian Yusoff ◽  
Nurul Hana Mokhtar Kamal ◽  
Hamidi Abdul Aziz
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Palm Oil ◽  
Pure Water ◽  
Starch Synthesis ◽  
Type Model ◽  
The Novel ◽  
Mixing Ratio ◽  
Ultra Pure Water ◽  
Oil Export

Abstract Malaysia is the 2nd largest in palm oil export, and after overcoming economic age (average 25 years), the palm oil trees entail to replace usually. Therefore, a massive quantity of palm oil trunk biomass, containing a significant amount of starch, is available as bio-waste annually. The efficient extraction of this starch (carbohydrate polymer) would be worthwhile concerning the environment, economy, conversion of biowaste to bioresources, and waste dumping challenges. Central composite design executed an experimental model design, evaluated the impacts of process variables and their interaction through response surface methodology to optimize the novel bisulfite steeping method for starch synthesis. Design-Expert software performed the data analysis. The developed quadratic models for four factors (Strength of Sodium bisulfite solution, steeping hour, mixing ratio with the bisulfite solution and ultra-pure water) and one response (%Yield), demonstrated that a significant starch yield (13.54%) is achievable utilizing 0.74% bisulfite solution, 5.6 steeping hours, for 1.6 and 0.6 mixing ratio with the bisulfite solution and ultra-pure water respectively. Experimental outcomes were quite consistent with the predicted model, which eventually sustains the significance of this method. Malvern Zetasizer test revealed a bimodal granular distribution for starch, with 7.15µm of hydrodynamic size. Starch morphology was determined by scanning electron microscopy. X-ray diffraction investigation exhibits an A-type model, specifying persistent characteristics of extracted starch.

Applying a Life‐Cycle Assessment to the Ultra Pure Water Process of Semiconductor Manufacturing

2005 ◽  
Vol 6 (3) ◽  
pp. 173-189 ◽  
Author(s):  
Shiaw‐Wen Tien ◽  
Yi‐Chan Chung ◽  
Chih‐Hung Tsai ◽  
Yung‐Kuang Yang ◽  
Min‐Chi Wu
Keyword(s):  
Life Cycle Assessment ◽  
Life Cycle ◽  
Semiconductor Manufacturing ◽  
Pure Water ◽  
Ultra Pure Water

Preparation of ZnO Nanoparticles by 1064/532nm Laser Ablation and Studying the Effect of the Ablation Wavelength

2021 ◽  
Vol 1021 ◽  
pp. 171-180
Author(s):  
Munaf S. Majeed ◽  
Rabea Q. Nafil ◽  
Marwa F. Abdul Jabbar ◽  
Kadhim H. Suffer
Keyword(s):  
Zinc Oxide ◽  
Laser Ablation ◽  
Pure Water ◽  
Pulsed Laser ◽  
Pulsed Laser Ablation ◽  
Ultra Pure Water ◽  
Hexagonal Shape ◽  
532 Nm ◽  
Laser Ablation Technique

We prepared Zinc oxide nanomaterial employing PLA (pulsed laser ablation) technique. A pure Zn target was immersed in ultra-pure water (UPW) and it was subjected to several pulses (1st. and 2nd. harmonic) of the pumping Nd: YAG laser. The influence of changing laser’s wavelength (1064, 532) nm on the characterization of the produced nanoparticles was studied. The results obtained from studying the structure, topography, and morphology of the product showed that the particles have a hexagonal shape. Also, changing the wavelength of the laser from 532nm to 1064nm leads to size reduction and density increasing of the nanoparticles.

Detoxification of Deoxynivalenol by 60Co γ-ray Irradiation and Toxicity Analyses of Radiolysis Products

2019 ◽  
Vol 102 (6) ◽  
pp. 1749-1755
Author(s):  
Mengmeng Li ◽  
Erqi Guan ◽  
Ke Bian
Keyword(s):  
Body Weight Gain ◽  
Pure Water ◽  
Farm Animals ◽  
Feed Consumption ◽  
Control Group ◽  
Ultra Pure Water ◽  
Radiolysis Products ◽  
Significant Toxicity ◽  
Γ Ray ◽  
Degradation Effect

Background: Deoxynivalenol (DON) is a type B trichothecene that occurs predominantly in grains such as wheat, maize, and barley and has been implicated in incidents of mycotoxicoses in both humans and farm animals. Objective: In the present study, we chose 60Co γ-ray irradiation to degrade DON. Methods: First, the degradation effect of irradiation on DON was analyzed. Second, the toxicity analyses of radiolysis products were studied by oral gavage. Results: The results indicated that 60Co γ-ray irradiation had significant degradation effect on pure DON: when 20 kGy γ-ray irradiation was used for 2 μg/mL DON in acetonitrile–water, the degradation efficiency of DON was 83%, and 2 μg/mL DON in ultra-pure water was completely degraded after 5 kGy γ-ray irradiation. The concentration of 200 μg/mL DON in ultra-pure water had significant toxicity to mice: decreased body weight gain and feed consumption as well as pathological changes in liver and kidney were observed compared with the control group. Conclusions: No significant toxicity was observed in mice that were given these degraded solutions treated by γ-ray irradiation, which indicated that the toxicity of radiolysis products in ultra-pure water had significantly decreased after treatment by γ-ray irradiation. Highlights: This research offered some reference to detoxify DON in cereal grains.

Luminescent magnetic nanoparticles encapsulated in MOFs for highly selective and sensitive detection of ClO−/SCN− and anti-counterfeiting

Nanoscale ◽  
2018 ◽  
Vol 10 (18) ◽  
pp. 8667-8676 ◽  
Author(s):  
Chaorui Li ◽  
Jun Hai ◽  
Siliang Li ◽  
Baodui Wang ◽  
Zhengyin Yang
Keyword(s):  
Magnetic Nanoparticles ◽  
Fluorescent Probe ◽  
Pure Water ◽  
High Sensitivity ◽  
Sensitive Detection ◽  
Zeolitic Imidazolate Framework ◽  
Framework Materials ◽  
Ultra Pure Water

Luminous Eu(iii) complex-functionalized Fe3O4 nanoparticles encapsulated into zeolitic imidazolate framework materials (nano-ZIF-8) were prepared and employed as a fluorescent probe for detecting ClO− and SCN− with good selectivity and high sensitivity in ultra-pure water.

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