Quality control of washed red blood cells: experience in determining residual protein

Author(s):  
O.V. Kozhemyako ◽  
◽  
Ya.E. Zhigalina ◽  
E.L. Zeiler ◽  
L.V. Golovanova ◽  
...  
2019 ◽  
Vol 64 (10) ◽  
pp. 599-602
Author(s):  
O. V. Petrova ◽  
G. R. Shabanova ◽  
O. I. Murygina ◽  
E. V. Panova ◽  
D. M. Nikulina

The purpose - evaluated the quality of the implementation of hematology studies using sigmametry. Studies were perfomed at the Federal state budgetary establishment “Federal center cardiovascular surgery”. The data were collected in the period for 2017 y. The following hematology parameters were monitored daily: white cells, red blood cell, platelet, hemoglobin. For conducting laboratory quality control we used certified three-level (Level 1 (low) , Level 2 (normal)and Level 3 (High)) control material “CBC-XE Hematology control” (“R and D Systems”, USA). The study of blood hematology parameters was carried out on an automatic hematology analyzer “Sysmex XT- 2000i” and “Sysmex XT- 1800i” (“Sysmex Corporation”, Japan). Sigmametry values were calculated the CV (coefficient of variation), the systematic error (Bias) and the total allowable analytical error (TE max). On the analyzer «Sysmex XT- 2000i» sigma values for platelet were between 3 and 4. For white blood cells, red blood cells, hemoglobin the value of the sigma was greater than 4. On the analyzer «Sysmex XT- 1800i» for platelet, white blood cells, red blood cells, hemoglobin the value of the sigma was greater than 4. Based on the results obtained, we drew conclusions on the choice of control material and the frequency of the internal laboratory quality control. The introduction of sigmametry in the daily activities of clinic laboratory will improve the quality of the determination of platelet, which requires further observation.


Transfusion ◽  
2007 ◽  
Vol 0 (0) ◽  
pp. 071121030748002-??? ◽  
Author(s):  
E.G. Góes ◽  
M.A. Ottoboni ◽  
P.V.B. Palma ◽  
F.R. Morais ◽  
C.A. Pelá ◽  
...  

2019 ◽  
Vol 71 (1) ◽  
pp. 93-101
Author(s):  
M.N.A. Marchi ◽  
P.E. Luz ◽  
R.R. Martins ◽  
S.M. Simonelli ◽  
U.P. Pereira ◽  
...  

ABSTRACT The objective of this study was to perform a quality control assessment of red blood cells after standardization of the blood production stages. For this purpose, separation of the blood components to obtain red blood cells, the storage of the blood packets and an evaluation of blood quality were performed. The mean (± SD) volume, globular volume, hemoglobin and hemolysis percentage of the red blood cell concentrate were 299.77±30.08mL, 60.87±2.60%, 20.57±0.93g/DL and 0.09±0.07%, respectively. The means (± SD) of the volume, globular volume, total hemoglobin percentage of hemolysis and hemoglobin per unit of packed red blood cells after the storage period (8.83±6.73 days) were 57.55±3.01%, 20.30±0.89 0, 20±0.12%, and 60.90±7.65. The red blood cell packets were within the parameters of quality control established by Health Ministry legislation in humans and allow us to conclude that the standardization of blood production stages involves the selection of donors until the end of storage and is necessary to produce quality red blood cells. Quality control aims to find possible flaws in the procedures to be repaired, increasing transfusion safety.


Transfusion ◽  
1981 ◽  
Vol 21 (5) ◽  
pp. 498-501 ◽  
Author(s):  
IO Szymanski ◽  
JM Harper ◽  
PR Odgren ◽  
CR Valeri

2018 ◽  
Vol 14 (1) ◽  
Author(s):  
C. Blasi Brugué ◽  
Rui R. F. Ferreira ◽  
I. Mesa Sanchez ◽  
Rita M. C. Graça ◽  
Inês M. Cardoso ◽  
...  

Transfusion ◽  
1981 ◽  
Vol 21 (3) ◽  
pp. 235-240 ◽  
Author(s):  
HT Meryman ◽  
M Hornblower

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