scholarly journals The influence of the stereotypic forms of activity in mice behaviour in standard behavioural tests

2021 ◽  
pp. 47-58
Author(s):  
Kseniya P. Avimova ◽  
Dmitry B. Sandakov
Keyword(s):  
Home Cage ◽  
Laboratory Animals ◽  
Immobility Time ◽  
Stereotypic Behaviour ◽  
Hole Board ◽  
Behavioural Test ◽  
Behavioural Tests ◽  
Home Cage Activity ◽  
Grooming Behaviour ◽  
Over Time

Laboratory animals often develop abnormal repetitive (stereotypic) behaviour that can influence both physiology and behavioural test results. Such abnormal behaviours usually develop in suboptimal environment and increase over time. To explore the development of stereotypic forms of activity night home-cage behaviour of laboratory mice was analysed and collated with the behaviour in open field (OFT), hole-board (HBT) and tail suspension (TST) tests twice within 16 weeks. Mice expressed few stereotypies and their duration lessened over time from median 8.2 to 1.0 %. In contrast, grooming behaviour increased significantly from 29.5 to 49.6 %. Home-cage grooming correlated with the latency of locomotion start in OFT and with the immobility time in TST. Intensity and stability of stereotypic activity and grooming inf­luenced the duration of grooming in OFT: the mice with unstable stereotypies groomed more than others, and mice with the lowes home-cage grooming level also groomed in OFT the most. Intensity and stability of night grooming influenced the behaviour in TST: the mice with unstable level of grooming were the most mobile in this test. Abnormal home-cage activity may indicate impaired welfare, and that, in turn, may affect test activity, so researchers need to keep it in mind when planning animal behaviour experiments.  

scholarly journals ANTI-PSYCHOTIC ACTIVITY OF PYRUS COMMUNIS JUICE

2017 ◽  
Vol 9 (4) ◽  
pp. 113
Author(s):  
Arzoo Singh Pannu ◽  
Milind Parle
Keyword(s):  
Experimental Models ◽  
Pyrus Communis ◽  
Anti Psychotic ◽  
Head Bobbing ◽  
Stereotypic Behaviour ◽  
Biochemical Estimation ◽  
Pear Juice ◽  
The Brain ◽  
Grooming Behaviour

Objective: The present study aim to investigate the anti-psychotic potential of pyrus communis in the rodents.Methods: The fresh juice of pyrus communis (Pear) was administered orally to rodents for 21 d and the anti-psychotic activity was assessed by in vitro methods viz ketamine induced stereotypic behaviour, pole climbing avoidance in rats and swim induced grooming behaviour experimental models. The biochemical estimation was done on 21 d.Results: The different concentrations of fresh pyrus communis juice was assayed. When pyrus communis juice (PCJ) was administered chronically for 21 d remarkably decreased ketamine induced falling, head-bobbing, weaving and turning counts. Administration of Pear juice significantly delayed the latency time taken by the animals to climb the pole in Cook’s pole climb apparatus. In swim induced grooming behaviour model, Pear juice significantly reduced swim induced grooming behaviour. Moreover, Pear juice significantly decreased the brain dopamine levels and inhibited acetyl cholinesterase activity. In the present study, Pear juice significantly enhanced reduced glutathione levels in the brains of mice, thereby reflecting enhanced scavenging of free radicals and in turn preventing occurrence of psychotic attack.Conclusion: The present study revealed that pyrus communis juice possessed significant anti-psychotic activity.

scholarly journals Impulsivity and home-cage activity are decreased by lentivirus-mediated silencing of serotonin transporter in the rat hippocampus

2013 ◽  
Vol 548 ◽  
pp. 38-43 ◽  
Author(s):  
Francesca Zoratto ◽  
Amanda L. Tringle ◽  
Giancarlo Bellenchi ◽  
Luisa Speranza ◽  
Domenica Travaglini ◽  
...  
Keyword(s):  
Serotonin Transporter ◽  
Home Cage ◽  
Rat Hippocampus ◽  
Cage Activity ◽  
Home Cage Activity

scholarly journals Influence of mouse strain, infective dose and larval burden in the brain on activity in Toxocara-infected mice

2001 ◽  
Vol 75 (1) ◽  
pp. 23-32 ◽  
Author(s):  
D.M. Cox ◽  
C.V. Holland
Keyword(s):  
Mouse Strain ◽  
Post Infection ◽  
Home Cage ◽  
Brain Activity ◽  
Toxocara Canis ◽  
Cage Activity ◽  
Home Cage Activity ◽  
The Brain ◽  
Larval Recovery ◽  
Larval Burden

Outbred LACA mice and inbred NIH mice were administered low (100 ova), medium (1000 ova), high (3000 ova) and trickle (4×250 ova) doses ofToxocara canisova and the effect of infection on activity was examined with respect to: (i) the dose of ova administered and (ii) the number of larvae recovered from the brain. Larval recovery from the brain was significantly reduced in NIH mice compared to LACA mice for the 1000, 3000 and trickle doses. Mice from each strain were divided into larval intensity groupings based upon the number of larvae recovered from their brain. Activity for each mouse was measured pre- and post-infection by observing its behaviour in the home cage. Activity was assessed by monitoring six different independent categories of murine behaviour – ambulation, grooming, rearing, digging, climbing and immobility. Within each behavioural category, the duration of time spent at each behaviour per mouse within one thousandth of a second, the number of short bouts performed and the number of long bouts of behaviour performed were recorded over a 20 min period. Activity of LACA and NIH mice differed prior to infection. LACA mice spent more time immobile compared to NIH mice, which ambulated and climbed more. Variations in activity were also observed between groups of mice prior to infection. The effect of infection differed by strain, by dose and by larval intensity. Post-infection LACA mice became more immobile and ambulated less. NIH mice showed reduced immobility, but while ambulation decreased digging and climbing increased post-infection. Short bouts of activity remained unchanged among LACA mice post-infection but showed an increase for some behaviours in NIH mice.

Home cage activity and behavioral performance in inbred and hybrid mice

2002 ◽  
Vol 136 (2) ◽  
pp. 555-569 ◽  
Author(s):  
Xiangdong Tang ◽  
Stuart M Orchard ◽  
Larry D Sanford
Keyword(s):  
Home Cage ◽  
Behavioral Performance ◽  
Cage Activity ◽  
Home Cage Activity ◽  
Hybrid Mice

Genetic study of multigenic factors related to strain difference of temporal pattern in mouse home-cage activity

2011 ◽  
Vol 71 ◽  
pp. e166
Author(s):  
Tatsuhiko Goto ◽  
Ayako Ishii ◽  
Akinori Nishi ◽  
Aki Takahashi ◽  
Toshihiko Shiroishi ◽  
...  
Keyword(s):  
Genetic Study ◽  
Temporal Pattern ◽  
Home Cage ◽  
Strain Difference ◽  
Cage Activity ◽  
Home Cage Activity

scholarly journals Home-cage activity in heterogeneous stock (HS) mice as a model of baseline activity

2002 ◽  
Vol 1 (3) ◽  
pp. 166-173 ◽  
Author(s):  
J. Mill ◽  
M. J. Galsworthy ◽  
J. L. Paya-Cano ◽  
F. Sluyter ◽  
L. C. Schalkwyk ◽  
...  
Keyword(s):  
Home Cage ◽  
Heterogeneous Stock ◽  
Cage Activity ◽  
Baseline Activity ◽  
Home Cage Activity

scholarly journals Chronic Stimulation of Arcuate Kiss1 Neurons Decreases Bone Mass in Female Mice

2021 ◽  
Vol 5 (Supplement_1) ◽  
pp. A232-A232
Author(s):  
Ruben Rodriguez ◽  
Candice B Herber ◽  
William C Krause ◽  
Holly A Ingraham
Keyword(s):  
Bone Mass ◽  
Home Cage ◽  
Designer Drugs ◽  
Reproductive Capacity ◽  
Estrogen Signaling ◽  
Female Mice ◽  
Cage Activity ◽  
Increased Risk ◽  
Chronic Activation ◽  
Home Cage Activity

Abstract Loss of peripheral estrogen in postmenopausal women is often associated with decreased physical activity and loss of bone mass, leading to an increased risk of metabolic diseases, osteoporosis, and skeletal fragility. While it is well-established that loss of peripheral estrogen signaling results in bone loss, we previously found that eliminating central estrogen signaling paradoxically results in an unexpected massive increase in bone mass only in female mice. Specifically, deletion of estrogen receptor alpha (ERα) signaling in kisspeptin 1 (Kiss1) expressing neurons of the arcuate nucleus (ARCKiss1) increases bone mass at the expense of reproduction in female mice. Currently, the mechanisms and the neurocircuits that modulate these unexpected responses are unknown. Here, to begin addressing these questions, we asked if changing the neuronal output of ARCKiss1 neurons using chemogenetic manipulation of ARCKiss1 neurons might also alter bone mass and locomotion in female mice. To do this, we delivered stimulatory (AAV2-hM3Dq-mCherry) designer receptors exclusively activated by designer drugs (DREADDs) to the ARC of wild type and Kiss1-Cre+ (Kiss1-CrehM3q-DREADDs) female mice and asked if chronic activation of ARCKiss1 neurons might alter bone mass as analyzed by standard ex-vivo µCT imaging. Clozapine N-oxide (CNO) was delivered for 22 days (0.1 mg/mL). We also leveraged the ANY-Maze system to assess home cage activity over an extensive 96-hour period. Acute activation of ARCKiss1 tended to decrease home cage activity by nearly 40% in Kiss1-CrehM3q-DREADDs mice during the dark period compared to WT females. Interestingly, chronic activation of ARCKiss1 neurons significantly lowered trabecular bone volume by nearly 30%. Current studies are underway to ask if inhibiting ARCKiss1 neurons results in increased bone mass. Our findings collectively suggest that the neuronal activity of ARCKiss1 neurons is sufficient to shift energy allocation away from locomotion and bone-building to maximize reproductive capacity. We speculate that the widely used SERM in breast cancer treatment, Tamoxifen, might exert its bone sparing effect by silencing ARCKiss1 neurons.

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