scholarly journals Retraction of: MicroRNA-141 Inhibits the Proliferation of Penile Cavernous Smooth Muscle Cells Associated with Down-Regulation of the Rhoa/Rho Kinase Signaling Pathway

2021 ◽  
Vol 55 (5) ◽  
pp. 671-671
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Signaling Pathway ◽  
Rho Kinase ◽  
Muscle Cells ◽  
Kinase Signaling ◽  
Down Regulation ◽  
Kinase Signaling Pathway ◽  
Cavernous Smooth Muscle

scholarly journals MicroRNA-141 Inhibits the Proliferation of Penile Cavernous Smooth Muscle Cells Associated with Down-Regulation of the Rhoa/Rho Kinase Signaling Pathway

2018 ◽  
Vol 48 (1) ◽  
pp. 348-360 ◽  
Author(s):  
Ying Zhang ◽  
Linpei Jia ◽  
Wei Ji ◽  
Hai Li
Keyword(s):  
Smooth Muscle ◽  
Erectile Dysfunction ◽  
Smooth Muscle Cells ◽  
Signaling Pathway ◽  
Rho Kinase ◽  
Muscle Cells ◽  
Kinase Signaling ◽  
Kinase Signaling Pathway ◽  
Cavernous Smooth Muscle

Background/Aims: The role of the RhoA/Rho kinase signaling pathway in diabetes mellitus-induced erectile dysfunction has been partially understood. Methods: In the present study, we explored the changes of the RhoA/Rho associated kinase (ROCK) signaling pathway in diabetic erectile dysfunction in vivo and the effects of microRNA-141 on the RhoA/ROCK signaling pathway in vitro. Results: The mRNA and protein expressions of RhoA and ROCK2 were significantly increased while the expression of microRNA-141 was decreased in the penile cavernous smooth muscle cells of rats with diabetic erectile dysfunction. Moreover, increased expression of microRNA-141, decreased expressions of RhoA and ROCK2 (mRNA and protein), accelerated cell proliferation rate and reduced cell apoptosis were found in the microRNA-141 mimics group and the siRNA-Rho group. The microRNA-141 expression in the microRNA-141 inhibitors + siRNA-Rho group was significantly decreased. microRNA-141 specifically bound to Rho-3’-UTR and down-regulated the expression of Rho gene at the post transcriptional level. Conclusion: Decreased expression of miR-141 is associated with up-regulation of RhoA and ROCK2 in the RhoA/ROCK signaling pathway in rats with diabetic erectile dysfunction. miR-141 inhibits the growth of penile cavernous smooth muscle cells associated with down-regulation of the RhoA/ROCK signaling pathway in vitro.

scholarly journals Simvastatin Increases Fibulin-2 Expression in Human Coronary Artery Smooth Muscle Cells via RhoA/Rho-Kinase Signaling Pathway Inhibition

PLoS ONE ◽  
2015 ◽  
Vol 10 (7) ◽  
pp. e0133875 ◽  
Author(s):  
Noemí Serra ◽  
Roser Rosales ◽  
Lluís Masana ◽  
Joan-Carles Vallvé
Keyword(s):  
Smooth Muscle ◽  
Coronary Artery ◽  
Smooth Muscle Cells ◽  
Signaling Pathway ◽  
Rho Kinase ◽  
Muscle Cells ◽  
Kinase Signaling ◽  
Human Coronary Artery ◽  
Pathway Inhibition ◽  
Kinase Signaling Pathway

EFFECT OF CAVERNOUS NEUROTOMY ON CORPUS CAVERNOSUM SMOOTH MUSCLE: ALTERATION IN MYOSIN ISOFORM COMPOSITION AND THE SPHINGOSINE-1-PHOSPHATE/RHO-KINASE SIGNALING PATHWAY

2009 ◽  
Vol 181 (4S) ◽  
pp. 297-297 ◽  
Author(s):  
Memduh Aydin ◽  
Rowena Chua ◽  
Xin-Hua Zhang ◽  
Arnold Melman ◽  
Michael E DiSanto
Keyword(s):  
Smooth Muscle ◽  
Signaling Pathway ◽  
Corpus Cavernosum ◽  
Rho Kinase ◽  
Kinase Signaling ◽  
Sphingosine 1 Phosphate ◽  
Kinase Signaling Pathway

Abstract 18390: Ang II activates the RhoA Exchange Factor Arhgef1 in Humans

Circulation ◽  
2014 ◽  
Vol 130 (suppl_2) ◽  
Author(s):  
Maria-Luigia Carbone ◽  
Jérémy Brégeon ◽  
Nabila Devos ◽  
Anne Blanchard ◽  
Michel Azizi ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Rho Kinase ◽  
Renin Angiotensin System ◽  
Muscle Cells ◽  
Ang Ii ◽  
Kinase Signaling ◽  
Angiotensin System ◽  
Normotensive Subjects

Introduction: Although a causative role for RhoA-Rho kinase signaling has been recognized in the development of human hypertension, the molecular mechanism(s) as well as the RhoA exchange factors (GEFs) responsible for the over activation of RhoA remain unknown. Arhgef1 has been recently identified as a RhoA GEF involved in Ang II-mediated regulation of vascular tone and hypertension in mice. Hypothesis: Here we assessed the hypothesis that Arhgef1 is activated and involved in the activation of RhoA-Rho kinase signaling by Ang II in humans. Methods: We used in vitro stimulation of human coronary artery smooth muscle cells and human peripheral blood cells (PBMC) by Ang II (0.1 μmol/L), and PBMC isolated from normotensive subjects before and after activation of the renin-angiotensin system by a low-salt diet for 7 days (checked by the increase in plasma aldosterone and active renine). Activation of Arhgef1 was monitored by measuring its tyrosine phosphorylation by western blot and phosphorylation of the Rho kinase target MYPT was used to measure the activity of RhoA/Rho kinase signaling. Results: In vitro, Ang II induced activation of Arhgef1 in human vascular smooth muscle cells and PBMC. Silencing of Arhgef1 expression by siRNA in human vascular smooth muscle cells inhibited Ang II-induced activation of RhoA-Rho kinase signaling (0.67±0.12 vs 3.21±0.91relative to unstimulated condition, n=4, P<0.05). In normotensive subjects, activation of the renin-angiotensin system by a low-salt diet stimulated Arhgef1 activity in PBMC (1.26±0.12-fold over basal level, n=39, P<0.05). This activation was associated with an increase activity of RhoA-Rho kinase signaling (1.33±0.14-fold over basal level, n=39, P<0.05). Conclusions: Our results show that Ang II stimulates Arhgef1 activity and strongly suggest that Arhgef1 mediates Ang II-induced RhoA activation in humans. Moreover, we show for the first time that measurement of RhoA GEF activity in PBMC might be a useful method to evaluate RhoA GEF activity in humans.

scholarly journals Effects of Estrogen, Raloxifene, and Levormeloxifene on the Expression of Rho-kinase Signaling Molecules in Urethral Smooth Muscle Cells

Urology ◽  
2010 ◽  
Vol 76 (6) ◽  
pp. 1517.e6-1517.e11 ◽  
Author(s):  
Nongxiu Ning ◽  
Guiting Lin ◽  
Tom F. Lue ◽  
Ching-Shwun Lin
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Rho Kinase ◽  
Muscle Cells ◽  
Signaling Molecules ◽  
Kinase Signaling ◽  
Urethral Smooth Muscle

Angiotensin II promotes differentiation of mouse embryonic stem cells to smooth muscle cells through PI3-kinase signaling pathway and NF-κB

2013 ◽  
Vol 85 (1-2) ◽  
pp. 41-54 ◽  
Author(s):  
Xiaoye Zheng ◽  
Yutao Wu ◽  
Liangfeng Zhu ◽  
Qishan Chen ◽  
Yijiang Zhou ◽  
...  
Keyword(s):  
Stem Cells ◽  
Smooth Muscle ◽  
Angiotensin Ii ◽  
Embryonic Stem Cells ◽  
Smooth Muscle Cells ◽  
Signaling Pathway ◽  
Embryonic Stem ◽  
Muscle Cells ◽  
Pi3 Kinase ◽  
Kinase Signaling Pathway
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