EFFECT OF CAVERNOUS NEUROTOMY ON CORPUS CAVERNOSUM SMOOTH MUSCLE: ALTERATION IN MYOSIN ISOFORM COMPOSITION AND THE SPHINGOSINE-1-PHOSPHATE/RHO-KINASE SIGNALING PATHWAY

2009 ◽  
Vol 181 (4S) ◽  
pp. 297-297 ◽  
Author(s):  
Memduh Aydin ◽  
Rowena Chua ◽  
Xin-Hua Zhang ◽  
Arnold Melman ◽  
Michael E DiSanto
Keyword(s):  
Smooth Muscle ◽  
Signaling Pathway ◽  
Corpus Cavernosum ◽  
Rho Kinase ◽  
Kinase Signaling ◽  
Sphingosine 1 Phosphate ◽  
Kinase Signaling Pathway

The influence of ethanol intake on RhoA/Rho kinase signaling pathway in corpus cavernosum of OLETF rats

2013 ◽  
Vol 45 (2) ◽  
pp. 429-438 ◽  
Author(s):  
Su Jin Kim ◽  
Woong Jin Bae ◽  
Jin Hee Han ◽  
Sung Hoo Hong ◽  
Sae Woong Kim ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Corpus Cavernosum ◽  
Rho Kinase ◽  
Ethanol Intake ◽  
Kinase Signaling ◽  
Oletf Rats ◽  
Kinase Signaling Pathway

scholarly journals Angiotensin II Silencing Alleviates Erectile Dysfunction Through Down-Regulating the Rhoa/Rho Kinase Signaling Pathway in Rats with Diabetes Mellitus

2018 ◽  
Vol 45 (1) ◽  
pp. 419-427 ◽  
Author(s):  
Ying Zhang ◽  
Linpei Jia ◽  
Ying Zhang ◽  
Wei Ji ◽  
Hai Li
Keyword(s):  
Diabetes Mellitus ◽  
Erectile Dysfunction ◽  
Signaling Pathway ◽  
Corpus Cavernosum ◽  
Rho Kinase ◽  
Control Group ◽  
Kinase Signaling ◽  
Sprague Dawley ◽  
Kinase Signaling Pathway

Background/Aims: We aim to explore the role of angiotensin (Ang)II and the RhoA/Rho kinase signaling pathway in the pathogenesis of erectile dysfunction in diabetes mellitus (DM). Methods: Male Sprague-Dawley (SD) rats were used for experiments and short hairpin RNA (shRNA) was used to silence the AngII gene. The erectile function of rats was observed and intracavernous pressure and mean arterial pressure (ICP/MAP) were measured after electrical stimulation. Relaxation and contraction of smooth muscle in the corpus cavernosum were tested. Western blotting and quantitative RT-PCR were applied to measure the expressions of RhoA, Rho-associated kinase (ROCK)1 and ROCK2. Radioimmunoassay was applied to detect the levels of AngII. Results: Rats in the control group had the most erectile times, followed by AngII-silenced rats with DMED and rats with DMED. Rats with DMED had worse ICP and MAP than AngII-silenced rats. The contraction ability was markedly improved and relaxation ability was decreased in AngII-silenced rats with DMED as compared with rats with DMED. The levels of AngII were significantly increased in DMED rats while significantly decreased after AngII silencing. The mRNA and proteins of RhoA and ROCK2 were expressed in a similar way. Conclusion: AngII silencing improves erectile dysfunction via down-regulating the RhoA/Rho kinase signaling pathway.

scholarly journals MicroRNA-141 Inhibits the Proliferation of Penile Cavernous Smooth Muscle Cells Associated with Down-Regulation of the Rhoa/Rho Kinase Signaling Pathway

2018 ◽  
Vol 48 (1) ◽  
pp. 348-360 ◽  
Author(s):  
Ying Zhang ◽  
Linpei Jia ◽  
Wei Ji ◽  
Hai Li
Keyword(s):  
Smooth Muscle ◽  
Erectile Dysfunction ◽  
Smooth Muscle Cells ◽  
Signaling Pathway ◽  
Rho Kinase ◽  
Muscle Cells ◽  
Kinase Signaling ◽  
Kinase Signaling Pathway ◽  
Cavernous Smooth Muscle

Background/Aims: The role of the RhoA/Rho kinase signaling pathway in diabetes mellitus-induced erectile dysfunction has been partially understood. Methods: In the present study, we explored the changes of the RhoA/Rho associated kinase (ROCK) signaling pathway in diabetic erectile dysfunction in vivo and the effects of microRNA-141 on the RhoA/ROCK signaling pathway in vitro. Results: The mRNA and protein expressions of RhoA and ROCK2 were significantly increased while the expression of microRNA-141 was decreased in the penile cavernous smooth muscle cells of rats with diabetic erectile dysfunction. Moreover, increased expression of microRNA-141, decreased expressions of RhoA and ROCK2 (mRNA and protein), accelerated cell proliferation rate and reduced cell apoptosis were found in the microRNA-141 mimics group and the siRNA-Rho group. The microRNA-141 expression in the microRNA-141 inhibitors + siRNA-Rho group was significantly decreased. microRNA-141 specifically bound to Rho-3’-UTR and down-regulated the expression of Rho gene at the post transcriptional level. Conclusion: Decreased expression of miR-141 is associated with up-regulation of RhoA and ROCK2 in the RhoA/ROCK signaling pathway in rats with diabetic erectile dysfunction. miR-141 inhibits the growth of penile cavernous smooth muscle cells associated with down-regulation of the RhoA/ROCK signaling pathway in vitro.

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