scholarly journals Molecular-Phylogenetic Research of the Genus Hypericum L. in Flora of Azerbaijan

2021 ◽  
Vol 7 (11) ◽  
pp. 22-27
Keyword(s):  
Experimental Study ◽  
Chloroplast Dna ◽  
Genomic Dna ◽  
Native Species ◽  
Leaf Tissue ◽  
Rflp Analysis ◽  
Flowering Plant ◽  
The Family ◽  
Pcr Rflp ◽  
Molecular Phylogenetic

Hypericum is one of the 100 largest flowering plant genera forming the family Hypericaceae Juss., which belongs to the clusioid clade of the Malpighiales. Hypericum is represented in Azerbaijan flora by 19 native species and 1 subspecies belonging to 7 taxonomic sections. The chloroplast DNA of 8 species from the genus was studied by PCR-RFLP analysis. Total genomic DNA was extracted from leaf tissue using the DNeasyPlantMini kit. (Qiagen Inc.; Valencia, CA, USA) following the supplied protocol and quanti field using a Nanodrop (Nanodrop Technologies; Wilmington, DE, USA) spectrophotometer. The article is part of an experimental study that comprises molecular-phylogenetic research of this genus in the flora of Azerbaijan.

scholarly journals PCR-RFLP Analysis of Chloroplast DNA Variations in the atpI-atpH Spacer Region of the Genus Camellia

2008 ◽  
Vol 77 (4) ◽  
pp. 408-417 ◽  
Author(s):  
Natsu Tanikawa ◽  
Takashi Onozaki ◽  
Masayoshi Nakayama ◽  
Michio Shibata
Keyword(s):  
Chloroplast Dna ◽  
Rflp Analysis ◽  
Pcr Rflp ◽  
Dna Variations

scholarly journals Early evolution of the angiosperm clade Asteraceae in the Cretaceous of Antarctica

2015 ◽  
Vol 112 (35) ◽  
pp. 10989-10994 ◽  
Author(s):  
Viviana D. Barreda ◽  
Luis Palazzesi ◽  
Maria C. Tellería ◽  
Eduardo B. Olivero ◽  
J. Ian Raine ◽  
...  
Keyword(s):  
Pollen Grains ◽  
Terrestrial Ecosystems ◽  
Flowering Plants ◽  
Flowering Plant ◽  
Recent Common Ancestor ◽  
Time Interval ◽  
Most Recent Common Ancestor ◽  
The Family ◽  
Dramatic Rise ◽  
Molecular Phylogenetic

The Asteraceae (sunflowers and daisies) are the most diverse family of flowering plants. Despite their prominent role in extant terrestrial ecosystems, the early evolutionary history of this family remains poorly understood. Here we report the discovery of a number of fossil pollen grains preserved in dinosaur-bearing deposits from the Late Cretaceous of Antarctica that drastically pushes back the timing of assumed origin of the family. Reliably dated to ∼76–66 Mya, these specimens are about 20 million years older than previously known records for the Asteraceae. Using a phylogenetic approach, we interpreted these fossil specimens as members of an extinct early diverging clade of the family, associated with subfamily Barnadesioideae. Based on a molecular phylogenetic tree calibrated using fossils, including the ones reported here, we estimated that the most recent common ancestor of the family lived at least 80 Mya in Gondwana, well before the thermal and biogeographical isolation of Antarctica. Most of the early diverging lineages of the family originated in a narrow time interval after the K/P boundary, 60–50 Mya, coinciding with a pronounced climatic warming during the Late Paleocene and Early Eocene, and the scene of a dramatic rise in flowering plant diversity. Our age estimates reduce earlier discrepancies between the age of the fossil record and previous molecular estimates for the origin of the family, bearing important implications in the evolution of flowering plants in general.

scholarly journals The use of PCR-RFLP to genetically distinguish the morphologically close species: Ceriodaphnia dubia Richard, 1894 and Ceriodaphnia silvestrii Daday, 1902 (Crustacea Cladocera)

2010 ◽  
Vol 70 (1) ◽  
pp. 121-124 ◽  
Author(s):  
MJ. Abreu ◽  
MJ. Santos-Wisniewski ◽  
O. Rocha ◽  
TC. Orlando
Keyword(s):  
Exotic Species ◽  
Native Species ◽  
Rflp Analysis ◽  
Ceriodaphnia Dubia ◽  
Molecular Techniques ◽  
Rrna Genes ◽  
Its2 Region ◽  
State Of Water ◽  
Pcr Rflp ◽  
Rna Genes

The cladocerans are important components of planktonic and benthic freshwater and good indicators of the trophic state of water bodies. The morphological taxonomy of many species of Cladocera is considered complex with minor differences separating some species. Nowadays, molecular techniques provide a powerful tool to identify and classify different taxonomical levels, using mainly ribosomal RNA genes (rRNA) as molecular markers. In the present work we performed PCR-RFLP to separate Ceriodaphnia dubia, an exotic species in Brazil and the native species Ceriodaphnia silvestrii, widely distributed in Brazilian freshwater. The RFLP analysis of the ITS1-5.8S-ITS2 region of rRNA genes showed to be different between C. dubia and C. silvestrii when using enzymes EcoRI, ApaI and SalI. Thus, the ITS1-5.8S-ITS2 region proved to be a useful molecular marker to differentiate the studied Ceriodaphnia species, which makes the task easier of telling apart species that are morphologically very similar. Also, this methodology might be interesting in determining the distribution of the exotic species C. dubia in Brazilian freshwaters, particularly in cases when C. dubia occurs in the absence of C. silvestrii, a particularly difficult task for ecologists who are not taxonomy specialists.

Detection of polymorphism of Calgranulin A gene in Indian Murrah Buffalo

2015 ◽  
Author(s):  
Sourabh Sulabh ◽  
Archana Verma ◽  
I. D. Gupta ◽  
S. Rajesh Kumar
Keyword(s):  
Immune System ◽  
Genetic Variants ◽  
Genomic Dna ◽  
Annealing Temperature ◽  
Rflp Analysis ◽  
Clinical Mastitis ◽  
Murrah Buffalo ◽  
Pcr Rflp ◽  
Pcr Products ◽  
Calgranulin A

Calgranulin A (S100A8) gene is one of the important candidate genes, which affects the host disease resistance by enhancing the immune system. Present study was undertaken with the objectives to identify polymorphism in Calgranulin A gene and to associate identified genetic variants with the incidence of clinical mastitis in Murrah buffalo. Genomic DNA was isolated from 100 randomly selected lactating Murrah. Two sets of primers were designed to amplify targeted regions of the gene. PCR products were obtained at annealing temperature of 63.8oC and were of 449 and 489 bp for respective primer set. PCR-RFLP analysis was carried out using HinfI for contig I and AluI and MboII restriction endonucleases for contig II. Both the contigs revealed monomorphism with frequency of the only prevailing A allele as 1.00. It was not feasible to analyze association with the incidence of mastitis, as no genetic variants were observed in the animals studied.

PCR-RFLP analysis of the whole chloroplast DNA from three cultivated species of cotton (Gossypium L.)

Euphytica ◽  
2007 ◽  
Vol 156 (1-2) ◽  
pp. 47-56 ◽  
Author(s):  
Rashid Ismael Hag Ibrahim ◽  
Jun-Ichi Azuma ◽  
Masahiro Sakamoto
Keyword(s):  
Chloroplast Dna ◽  
Rflp Analysis ◽  
Pcr Rflp ◽  
Cultivated Species

scholarly journals Identification of chloroplast DNA variations by PCR-RFLP analysis in Dendranthema

2003 ◽  
Vol 72 (3) ◽  
pp. 197-204 ◽  
Author(s):  
Sanae Kishimoto ◽  
Ryutaro Aida ◽  
Michio Shibata
Keyword(s):  
Chloroplast Dna ◽  
Rflp Analysis ◽  
Pcr Rflp ◽  
Dna Variations
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