scholarly journals Freeze-Float System for High-throughput Measurement of Ice Nucleation

2021 ◽  
Author(s):  
Yuki Kamijo ◽  
Ratmir Derda
Keyword(s):  
Temperature Distribution ◽  
Sample Size ◽  
High Throughput ◽  
High Throughput Screening ◽  
Cooling System ◽  
Ice Nucleation ◽  
Uniform Temperature ◽  
Detection Program ◽  
Accuracy Of Measurement ◽  
Platform System

In this publication, we developed the high throughput screening implementation of freeze-float selection platform system we established in the previous publication. The goal of this publication is to expand the system to higher throughput and accuracy. In the following sections, we describe the steps for automated droplets generations, adaptation of freeze-float selection using controlled and uniform temperature cooling system, and semi-automated droplets detection program. We aimed to improve previously published system to add functional advantages, such as; a) increased efficiency of the screening with fewer manipulation steps; b) increased accuracy of measurement due to the increased sample size; c) increased uniformity of temperature distribution by incorporating the controlled-rate freezer. In the following sections, we describe the steps for automated droplets generations, freeze-float system adaptations using controlled and uniform temperature cooling system, and semi-automated droplets detection program.

A Thermal System for a High Throughput Continuous Flow Polymerase Chain Reaction Device (CFPCR)

2008 ◽  
Author(s):  
P.-C. Chen ◽  
D. S. Park ◽  
B. H. You ◽  
N. Kim ◽  
T. Park ◽  
...  
Keyword(s):  
Temperature Distribution ◽  
High Throughput ◽  
Continuous Flow ◽  
Thermal Environment ◽  
Infrared Camera ◽  
Thermal System ◽  
Uniform Temperature ◽  
Chain Reaction ◽  
Copper Strip ◽  
Polymerase Chain

A thermal system used to evaluate a high throughput 96 continuous flow polymerase chain reactor (CFPCR) array was designed, fabricated, and tested. Each polymerase chain reactor (PCR) in the array required three different temperature zones to realize denaturaiton at 90°C–94°C, renaturation at 50°C–70°C, and extension at 72°C; a total of 288 temperature zones were required for the 96 CFPCR array. In an initial configuration, 18 copper strips were used to define the 288 temperature zones. Each copper strip was controlled by a PID feedback control loop. Numerical simulations were used to understand the thermal crosstalk phenomena between the micromilled copper strips, which were tightly packed since the high throughput micro-titer plate format restricted each CFPCR to a square 8 mm on a side. The lowest achievable temperature in each renaturation zone in this complicated thermal environment was also identified. Thermal crosstalk limited the minimum renaturation temperature to 61.1°C. An infrared camera was used to investigate the temperature uniformity over a 0.25 mm thick polycarbonate sheet mounted on the thermal system. The temperature distribution was not uniform due to poor contact between the copper strips and device, warm air accumulated between the packed copper strips, and greater heat transfer around the boundaries of the device. More work is required to overcome these limitations and achieve a more uniform temperature distribution for a multi well CFPCR.

Application of the GUS marker gene technique to high-throughput screening of rhizobial competition

1999 ◽  
Vol 45 (8) ◽  
pp. 678-685 ◽  
Author(s):  
Kate J Wilson ◽  
Adriana Parra ◽  
Lina Botero
Keyword(s):  
Phaseolus Vulgaris ◽  
Sample Size ◽  
Microbial Ecology ◽  
High Throughput ◽  
High Throughput Screening ◽  
Marker Gene ◽  
Nodule Occupancy ◽  
Gus Gene ◽  
Gus Assay ◽  
Rhizobium Strains

The GUS marker gene system has been developed for the study of bacterial ecology, particularly rhizobial competition. For high-throughput field screening of rhizobial competition, the technique must be robust and reliable under diverse conditions, with diverse cultivars and strains. Here we demonstrate its applicability to the evaluation of competition on five different Phaseolus vulgaris cultivars with 10 different Rhizobium strains. We describe refinements of the GUS assay, which make it more affordable and applicable to field-based studies, and use the assay to examine the effect of sample size on the accuracy of nodule occupancy measurements.Key words: GUS gene, Rhizobium, rhizobial competition, microbial ecology.

Mesoscopic Numerical Simulation of Temperature Crack with Non-Uniform Temperature Distribution in Concrete

2013 ◽  
Vol 477-478 ◽  
pp. 1014-1018 ◽  
Author(s):  
Yin Duan ◽  
Chao Zhang ◽  
Xiaolin Chang
Keyword(s):  
Temperature Distribution ◽  
Crack Propagation ◽  
Cooling System ◽  
Mass Concrete ◽  
Uniform Temperature ◽  
Heterogeneous Model ◽  
Uniform Temperature Distribution ◽  
Three Phase ◽  
Propagation Law ◽  
Random Aggregate

The heat-fluid coupling method is introduced to perform the thermal analysis of a pipe cooling system in mass concrete structures, to precisely simulate the distribution of temperature. The heterogeneous mesoscopic mechanical model based on the random aggregate-interface-mortar three-phase concrete is established. And numerical experiments of concrete water process are taken with a three-phase heterogeneous model, so as to actually simulate the whole crack propagation process considering non-uniform temperature distribution, and then explore the crack propagation law, generating mechanism and comprehensive understand the factors and process of the concrete temperature cracks under complex conditions.

scholarly journals Twin-plate Ice Nucleation Assay (TINA) with infrared detection for high-throughput droplet freezing experiments with biological ice nuclei in laboratory and field samples

2018 ◽  
Vol 11 (11) ◽  
pp. 6327-6337 ◽  
Author(s):  
Anna T. Kunert ◽  
Mark Lamneck ◽  
Frank Helleis ◽  
Ulrich Pöschl ◽  
Mira L. Pöhlker ◽  
...  
Keyword(s):  
Pseudomonas Syringae ◽  
High Throughput ◽  
Atmospheric Aerosols ◽  
Cooling System ◽  
Infrared Detector ◽  
Ice Nucleation ◽  
Cumulative Number ◽  
Ice Nuclei ◽  
Wide Range ◽  
Field Samples

Abstract. For efficient analysis and characterization of biological ice nuclei under immersion freezing conditions, we developed the Twin-plate Ice Nucleation Assay (TINA) for high-throughput droplet freezing experiments, in which the temperature profile and freezing of each droplet is tracked by an infrared detector. In the fully automated setup, a couple of independently cooled aluminum blocks carrying two 96-well plates and two 384-well plates, respectively, are available to study ice nucleation and freezing events simultaneously in hundreds of microliter-range droplets (0.1–40 µL). A cooling system with two refrigerant circulation loops is used for high-precision temperature control (uncertainty <0.2 K), enabling measurements over a wide range of temperatures (∼ 272–233 K) at variable cooling rates (up to 10 K min−1). The TINA instrument was tested and characterized in experiments with bacterial and fungal ice nuclei (IN) from Pseudomonas syringae (Snomax®) and Mortierella alpina, exhibiting freezing curves in good agreement with literature data. Moreover, TINA was applied to investigate the influence of chemical processing on the activity of biological IN, in particular the effects of oxidation and nitration reactions. Upon exposure of Snomax® to O3 and NO2, the cumulative number of IN active at 270–266 K decreased by more than 1 order of magnitude. Furthermore, TINA was used to study aqueous extracts of atmospheric aerosols, simultaneously investigating a multitude of samples that were pre-treated in different ways to distinguish different kinds of IN. For example, heat treatment and filtration indicated that most biological IN were larger than 5 µm. The results confirm that TINA is suitable for high-throughput experiments and efficient analysis of biological IN in laboratory and field samples.

scholarly journals Twin-plate ice nucleation assay (TINA) with infrared detection for high-throughput droplet freezing experiments with biological ice nuclei in laboratory and field samples

2018 ◽  
Author(s):  
Anna T. Kunert ◽  
Mark Lamneck ◽  
Frank Helleis ◽  
Mira L. Pöhlker ◽  
Ulrich Pöschl ◽  
...  
Keyword(s):  
High Throughput ◽  
Cooling System ◽  
Infrared Detector ◽  
Ice Nucleation ◽  
Infrared Detection ◽  
Ice Nuclei ◽  
Field Samples ◽  
Precision Temperature ◽  
Immersion Freezing

Abstract. For efficient analysis and characterization of biological ice nuclei under immersion freezing conditions, we developed a Twin-plate Ice Nucleation Assay (TINA) for high-throughput droplet freezing experiments, in which the temperature gradient and freezing of each droplet is tracked by an infrared detector. In the fully automated setup, a couple of independently cooled aluminum blocks carrying two 96-well plates and two 384-well plates, respectively, are available to study ice nucleation and freezing events simultaneously in hundreds of microliter range droplets (0.1–40 µL). A cooling system with two refrigerant circulation loops is used for high-precision temperature control (deviations

High-throughput screening program for botanical extracts

Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
L Hingorani ◽  
NP Seeram ◽  
B Ebersole
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Screening Program ◽  
Botanical Extracts

High throughput screening of microbial biodiversity for the discovery of novel cosmeceutical agents

Planta Medica ◽  
2015 ◽  
Vol 81 (16) ◽  
Author(s):  
K Georgousaki ◽  
N DePedro ◽  
AM Chinchilla ◽  
N Aliagiannis ◽  
F Vicente ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Microbial Biodiversity

High throughput screening to investigate Brazilian Cerrado biome plant extract bank chemical diversity

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
LS Espindola ◽  
RG Dusi ◽  
KR Gustafson ◽  
J McMahon ◽  
JA Beutler
Keyword(s):  
High Throughput ◽  
Plant Extract ◽  
High Throughput Screening ◽  
Chemical Diversity ◽  
Brazilian Cerrado ◽  
Cerrado Biome

High-throughput screening revealed a clinically relevant drug to induce sperm motility

2014 ◽  
Author(s):  
Clair Cochrane ◽  
Halil Ruso ◽  
Anthony Hope ◽  
Rosemary G Clarke ◽  
Christopher Barratt ◽  
...  
Keyword(s):  
Sperm Motility ◽  
High Throughput ◽  
High Throughput Screening
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