scholarly journals Aptamer Functionalized Upconversion Nanotheranostic Agent With Nuclear Targeting as the Highly Localized Drug-Delivery System of Doxorubicin

Author(s):  
Xinyue Song ◽  
Tao Yan ◽  
Feng Tian ◽  
Fengyan Li ◽  
Linlin Ren ◽  
...  

As a widely used anticancer drug, doxorubicin (DOX) could induce cell death mainly via interfering with DNA activity; thus, DOX could perform therapeutic effects mainly in the cell nucleus. However, most of the reported drug delivery systems lacked the well localization in the nucleus and released DOX molecules into the cytoplasm. Due to formidable barriers formed in the nuclear envelope, only around 1% of DOX could reach the nucleus and keep active. Therefore, DOX molecules were inevitably overloaded to achieve the desired therapeutic efficacy, which would induce serious side effects. Herein, we developed a highly localized drug nanocarrier for in situ release of DOX molecules to their action site where they could directly interfere with the DNA activity. In this work, we used cationic polymer-modified upconversion nanoparticles (UCNPs) as the luminescence core and gene carrier, while aptamers served as the DNA nanotrain to load DOX. Finally, the prepared nanotheranostic agent displayed good targetability, high cell apoptosis ratio (93.04%) with quite lower concentration than the LC50 of DOX, and obvious inhibition on tumor growth.

Author(s):  
MRINMOY DEKA ◽  
ABDUL BAQUEE AHMED ◽  
JASHOBIR CHAKRABORTY

Eye is a sensitive organ and is easily injured and infected. Delivery of drugs into eye is complicated due to removal mechanism of precorneal area results decrease in therapeutic response. Conventional ocular delivery systems like solution, suspension, ointment shows some disadvantages such as rapid corneal elimination, repeated instillation of drug and short duration of action. In situ polymeric delivery system will help to achieve optimal concentration of drug at the target site, thereby helps to achieve the desired therapeutic concentration. There are various novel ocular drug delivery systems such as In-situ gel, dendrimers, niosomes, nanoparticulate system, collagen shield, ocular iontophoresis suspension and ocusert etc. In situ gelling systems are liquid upon instillation and undergo a phase transition to form gel due to some stimuli responses such as temperature modulation, change in pH and presence of ions. Various attempts have been made towards the development of stable sustained release in-situ gels. Newer research in ophthalmic drug delivery systems is directed towards an incorporation of several drug delivery technologies, that includes to build up systems which is not only extend the contact time of the vehicle at the ocular surface, but which at the same time slow down the removal of the drug. This is a review based on ocular in situ gels, characteristization, techniques and evaluation of in situ ophthalmic drug delivery systems,


2007 ◽  
Vol 83A (3) ◽  
pp. 656-666 ◽  
Author(s):  
H. Ueda ◽  
M.C. Hacker ◽  
A. Haesslein ◽  
S. Jo ◽  
D.M. Ammon ◽  
...  

2018 ◽  
Vol 11 (1) ◽  
pp. 380
Author(s):  
Harsha Vardhani Kondepati ◽  
Girish Pai Kulyadi ◽  
Vamshi Krishna Tippavajhala

Sign in / Sign up

Export Citation Format

Share Document