scholarly journals Role of GRK2 in Trophoblast Necroptosis and Spiral Artery Remodeling: Implications for Preeclampsia Pathogenesis

2021 ◽  
Vol 9 ◽  
Author(s):  
Zi Lv ◽  
Li-ling Xiong ◽  
Xian Qin ◽  
Hua Zhang ◽  
Xin Luo ◽  
...  
Keyword(s):  
Spiral Artery ◽  
Artery Remodeling

Graphical AbstractLv et al. show that trophoblastic GRK2 deficiency could promote placenta dysfunction and PE-like phenotype by activating necroptosis in trophoblasts, then inducing cytokine disturbance in circulation.

scholarly journals 4307 Role of Pre-pregnancy Uterine Natural Killer Cells in Human Embryo Implantation

2020 ◽  
Vol 4 (s1) ◽  
pp. 102-102
Author(s):  
Jessica Kanter ◽  
Sneha Mani ◽  
Scott Gordon ◽  
Monica Mainigi
Keyword(s):  
Natural Killer ◽  
Tunel Staining ◽  
Spiral Artery ◽  
Secretory Phase ◽  
Potential Marker ◽  
Unk Cells ◽  
Artery Remodeling ◽  
Unk Cell ◽  
Uterine Natural Killer

OBJECTIVES/GOALS: Human placentation requires complex coordination between maternal and fetal cell types but remains incompletely understood. We hypothesize that uterine natural killer (uNK) cells, an immune cell type that increases in abundance during the implantation window, is essential for appropriate implantation and placentation. METHODS/STUDY POPULATION: We plan to examine stromal cell (SC) decidualization, spiral artery remodeling, and EVT invasion, processes vital for early pregnancy establishment, in the presence or absence of secretory phase uNK cells. Fetal extravillous trophoblasts (EVTs) will be isolated from first trimester pregnancy tissue; maternal SCs, endothelial cells (ECs) and uNK cells will be obtained from secretory phase uterine tissue. SCs will be placed in monoculture and coculture with uNK cells and prolactin will be measured to evaluate decidualization. To study EVT invasion, we will utilize our novel “implantation-on-a-chip” device to determine how addition of uNK cells affects EVT migration through a collagen-matrigel matrix. In this system, we will also examine spiral artery remodeling with or without uNK cells via TUNEL staining. RESULTS/ANTICIPATED RESULTS: We anticipate that uNK cell addition to SCs will lead to a significant increase in SC prolactin levels, suggesting a role of uNK cells in endometrial decidualization. In vitro, we expect the addition of uNK cells will increase EC apoptosis and promote EVT invasion. DISCUSSION/SIGNIFICANCE OF IMPACT: Although decidual NK cells are known to participate in placentation, the role of pre-pregnancy uNK cells is unknown. uNK cell involvement in processes important for the earliest stages of pregnancy would provide a potential marker for abnormal placentation and offer avenues for intervention to decrease placentation associated perinatal morbidity.

Possible role of CD59 in spiral artery remodeling.

Placenta ◽  
2015 ◽  
Vol 36 (10) ◽  
pp. A4-A5
Author(s):  
Masashi Ueda ◽  
Akihito Horie ◽  
Yukiyasu Sato ◽  
Koh Suginami ◽  
Hirohiko Tani ◽  
...  
Keyword(s):  
Spiral Artery ◽  
Artery Remodeling

scholarly journals Inhibition of Trophoblast-Induced Spiral Artery Remodeling Reduces Placental Perfusion in Rat Pregnancy

Hypertension ◽  
2010 ◽  
Vol 56 (2) ◽  
pp. 304-310 ◽  
Author(s):  
Stefan Verlohren ◽  
Nele Geusens ◽  
Jude Morton ◽  
Iris Verhaegen ◽  
Lydia Hering ◽  
...  
Keyword(s):  
Spiral Artery ◽  
Placental Perfusion ◽  
Artery Remodeling
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