scholarly journals Editorial: Chromatin Regulation in Cell Fate Decisions

Author(s):  
Justin Brumbaugh ◽  
Bruno Di Stefano ◽  
José Luis Sardina
2021 ◽  
Author(s):  
Peter Zeller ◽  
Jake Yeung ◽  
Buys Anton de Barbanson ◽  
Helena Vinas Gaza ◽  
Maria Florescu ◽  
...  

Post-translational histone modifications modulate chromatin packing to regulate gene expression. How chromatin states, at euchromatic and heterochromatic regions, underlie cell fate decisions in single cells is relatively unexplored. We develop sort assisted single-cell chromatin immunocleavage (sortChIC) and map active (H3K4me1 and H3K4me3) and repressive (H3K27me3 and H3K9me3) histone modifications in hematopoietic stem and progenitor cells (HSPCs), and mature blood cells in the mouse bone marrow. During differentiation, HSPCs acquire distinct active chromatin states that depend on the specific cell fate, mediated by cell type-specifying transcription factors. By contrast, most regions that gain or lose repressive marks during differentiation do so independent of cell fate. Joint profiling of H3K4me1 and H3K9me3 demonstrates that cell types within the myeloid lineage have distinct active chromatin but share similar myeloid-specific heterochromatin-repressed states. This suggests hierarchical chromatin regulation during hematopoiesis: heterochromatin dynamics define differentiation trajectories and lineages, while euchromatin dynamics establish cell types within lineages.


Sign in / Sign up

Export Citation Format

Share Document