scholarly journals Comparative Performance of the MGISEQ-2000 and Illumina X-Ten Sequencing Platforms for Paleogenomics

2021 ◽  
Vol 12 ◽  
Author(s):  
Kongyang Zhu ◽  
Panxin Du ◽  
Jianxue Xiong ◽  
Xiaoying Ren ◽  
Chang Sun ◽  
...  
Keyword(s):  
Error Rate ◽  
Comparative Performance ◽  
Lower Error ◽  
Human Dna ◽  
Duplication Rate ◽  
Lower Error Rate ◽  
Sequencing Bias ◽  
Comparable Performance ◽  
Library Complexity ◽  
Sequencing Platforms

The MGISEQ-2000 sequencer is widely used in various omics studies, but the performance of this platform for paleogenomics has not been evaluated. We here compare the performance of MGISEQ-2000 with the Illumina X-Ten on ancient human DNA using four samples from 1750BCE to 60CE. We found there were only slight differences between the two platforms in most parameters (duplication rate, sequencing bias, θ, δS, and λ). MGISEQ-2000 performed well on endogenous rate and library complexity although X-Ten had a higher average base quality and lower error rate. Our results suggest that MGISEQ-2000 and X-Ten have comparable performance, and MGISEQ-2000 can be an alternative platform for paleogenomics sequencing.

Situational Awareness in Map Displays

1987 ◽  
Vol 31 (5) ◽  
pp. 533-535 ◽  
Author(s):  
William P. Marshak ◽  
Gilbert Kuperman ◽  
Eric G. Ramsey ◽  
Denise Wilson
Keyword(s):  
Error Rate ◽  
Situational Awareness ◽  
Subjective Ratings ◽  
Subjective Data ◽  
Lower Error ◽  
Lower Error Rate ◽  
Moving Plane ◽  
Simple Paths ◽  
Relationship Of ◽  
The Relationship

The effectiveness of ego-centered (moving map) and earth-centered (moving plane) displays was studied with subjects monitoring an animated aircraft situational awareness display. Other independent variables were subject experience (aircrew vs non-aircrew) and path complexity (straight vs turning). Periodically, the display blanked and probe questions were asked concerning the relationship of the aircraft to the simulated world. Questions included judgements about angles, distances, time and terrain. Simple paths elicited a 28 percent lower error rate than did complex paths. Moving map displays had a 32 percent lower error rate than moving plane displays. No other significant effects were observed. Subjective ratings by subjects after the experiment revealed unanimous preference for the moving plane display and that the moving plane condition was believed to be easier! This contradiction indicates subjective data is limited in determining display effectiveness.

scholarly journals Comparative performance of the BGI and Illumina sequencing technology for single-cell RNA-sequencing

2019 ◽  
Author(s):  
Anne Senabouth ◽  
Stacey Andersen ◽  
Qianyu Shi ◽  
Lei Shi ◽  
Ping Liu ◽  
...  
Keyword(s):  
Single Cell ◽  
Rna Sequencing ◽  
High Throughput ◽  
Sequence Data ◽  
High Capacity ◽  
Read Depth ◽  
Comparative Performance ◽  
Single Cell Rna Sequencing ◽  
Comparable Performance ◽  
Sequencing Platforms

AbstractThe libraries generated by high-throughput single cell RNA-sequencing platforms such as the Chromium from 10X Genomics require considerable amounts of sequencing, typically due to the large number of cells. The ability to use this data to address biological questions is directly impacted by the quality of the sequence data. Here we have compared the performance of the Illumina NextSeq 500 and NovaSeq 6000 against the BGI MGISEQ-2000 platform using identical Single Cell 3’ libraries consisting of over 70,000 cells. Our results demonstrate a highly comparable performance between the NovaSeq 6000 and MGISEQ-2000 in sequencing quality, and cell, UMI, and gene detection. However, compared with the NextSeq 500, the MGISEQ-2000 platform performs consistently better, identifying more cells, genes, and UMIs at equalised read depth. We were able to call an additional 1,065,659 SNPs from sequence data generated by the BGI platform, enabling an additional 14% of cells to be assigned to the correct donor from a multiplexed library. However, both the NextSeq 500 and MGISEQ-2000 detected similar frequencies of gRNAs from a pooled CRISPR single cell screen. Our study provides a benchmark for high capacity sequencing platforms applied to high-throughput single cell RNA-seq libraries.

Research of the Flow Sampling Algorithm

2012 ◽  
Vol 472-475 ◽  
pp. 2588-2591
Author(s):  
Yan Liu
Keyword(s):  
Error Rate ◽  
Flow Measurement ◽  
Sampling Method ◽  
Sample Space ◽  
Sampling Algorithm ◽  
Fine Grained ◽  
Lower Error ◽  
Lower Error Rate

This article presents a flow measurement sampling method based on the application group, which identify the received message and then trasmit the identified packets to the corresponding application packet sample space, after this sampling each space packet independently. Thus to ensure the network to restore the situation in the application of fine-grained has lower error rate, thereby reducing the distortion of the application distribution.

scholarly journals Detecting Image Splicing Using Merged Features in Chroma Space

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Bo Xu ◽  
Guangjie Liu ◽  
Yuewei Dai
Keyword(s):  
Error Rate ◽  
Previous Literature ◽  
Experimental Results ◽  
Image Editing ◽  
Image Splicing ◽  
Lower Error ◽  
Lower Error Rate ◽  
Image Dataset ◽  
Splicing Detection ◽  
Markov Features

Image splicing is an image editing method to copy a part of an image and paste it onto another image, and it is commonly followed by postprocessing such as local/global blurring, compression, and resizing. To detect this kind of forgery, the image rich models, a feature set successfully used in the steganalysis is evaluated on the splicing image dataset at first, and the dominant submodel is selected as the first kind of feature. The selected feature and the DCT Markov features are used together to detect splicing forgery in the chroma channel, which is convinced effective in splicing detection. The experimental results indicate that the proposed method can detect splicing forgeries with lower error rate compared to the previous literature.

scholarly journals Sequencing of Sars-CoV-2 genome using different Nanopore chemistries

2021 ◽  
Author(s):  
Oscar Gonzalez-Recio ◽  
Monica Gutierrez-Rivas ◽  
Ramon Peiro-Pastor ◽  
Pilar Aguilera-Sepulveda ◽  
Cristina Cano-Gomez ◽  
...  
Keyword(s):  
Error Rate ◽  
Consensus Sequence ◽  
Flow Cell ◽  
Epidemiological Surveillance ◽  
Nanopore Sequencing ◽  
Efficient Tool ◽  
Flow Cells ◽  
Lower Error ◽  
Lower Error Rate ◽  
Cost Efficient

Nanopore sequencing has emerged as a rapid and cost-efficient tool for diagnostic and epidemiological surveillance of SARS-CoV-2 during the COVID-19 pandemic. This study compared results from sequencing the SARS-CoV-2 genome using R9 vs R10 flow cells and Rapid Barcoding Kit (RBK) vs Ligation Sequencing Kit (LSK). The R9 chemistry provided a lower error rate (3.5%) than R10 chemistry (7%). The SARS-CoV-2 genome includes few homopolymeric regions. Longest homopolymers were composed of 7 (TTTTTTT) and 6 (AAAAAA) nucleotides. The R10 chemistry resulted in a lower rate of deletions in timine and adenine homopolymeric regions than R9, at expenses of a larger rate (~10%) of mismatches in these regions. The LSK had a larger yield than RBK, and provided longer reads than RBK. It also resulted in a larger percentage of aligned reads (99% vs 93%) and also in a complete consensus genome. The results from this study suggest that the LSK used on a R9 flow cell could maximize the yield and accuracy of the consensus sequence when used in epidemiological surveillance of SARS-CoV-2.

scholarly journals Taking the perspectives of many people: humanization matters

2020 ◽  
Author(s):  
Tian Ye ◽  
Fumikazu Furumi ◽  
Daniel Catarino da Silva ◽  
Antonia Hamilton
Keyword(s):  
Virtual Reality ◽  
Mental Rotation ◽  
Error Rate ◽  
Social Contexts ◽  
Virtual Human ◽  
Human Agent ◽  
Lower Error Rate ◽  
Rate Experiment ◽  
Group Member ◽  
Director Task

In a busy space, people encounter many other people with different viewpoints, but classic studies of VPT examine only one agent at a time. This paper explores the issue of selectivity in VPT when different people are available to interact with. We consider the hypothesis that humanisation impacts on VPT in four studies using virtual reality methods. Experiment 1 & 2 use the Director Task to show that for more humanised agents (an in-group member or a virtual human agent), participants were more likely to use VPT to achieve lower error rate. Experiment 3 & 4 used a two-agent social mental rotation task to show that participants are faster and more accurate to recognise items which are oriented towards a more humanised agent (an in-group member or a naturally-moving agent). All results support the claim that humanisation alters the propensity to engage in VPT in rich social contexts.

scholarly journals Erratum to: Comparative performance of the BGISEQ-500 vs Illumina HiSeq2500 sequencing platforms for palaeogenomic sequencing

GigaScience ◽  
2018 ◽  
Vol 7 (12) ◽  
Author(s):  
Sarah Siu Tze Mak ◽  
Shyam Gopalakrishnan ◽  
Christian Carøe ◽  
Chunyu Geng ◽  
Shanlin Liu ◽  
...  
Keyword(s):  
Comparative Performance ◽  
Sequencing Platforms
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