scholarly journals Differential Patterns of IgG Subclass Responses to Plasmodium falciparum Antigens in Relation to Malaria Protection and RTS,S Vaccination

2019 ◽  
Vol 10 ◽  
Author(s):  
Carlota Dobaño ◽  
Rebeca Santano ◽  
Marta Vidal ◽  
Alfons Jiménez ◽  
Chenjerai Jairoce ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Igg Subclass ◽  
Malaria Protection

scholarly journals Age-Dependent IgG Subclass Responses to Plasmodium falciparum EBA-175 Are Differentially Associated with Incidence of Malaria in Mozambican Children

2011 ◽  
Vol 19 (2) ◽  
pp. 157-166 ◽  
Author(s):  
Carlota Dobaño ◽  
Diana Quelhas ◽  
Llorenç Quintó ◽  
Laura Puyol ◽  
Elisa Serra-Casas ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Malaria Incidence ◽  
Controlled Trial ◽  
Intermittent Preventive Treatment ◽  
Antibody Test ◽  
Merozoite Surface Protein ◽  
Blood Stage ◽  
Enzyme Linked Immunosorbent Assay ◽  
Igg Subclass ◽  
Content Type

ABSTRACTPlasmodium falciparumblood-stage antigens such as merozoite surface protein 1 (MSP-1), apical membrane antigen 1 (AMA-1), and the 175-kDa erythrocyte binding antigen (EBA-175) are considered important targets of naturally acquired immunity to malaria. However, it is not clear whether antibodies to these antigens are effectors in protection against clinical disease or mere markers of exposure. In the context of a randomized, placebo-controlled trial of intermittent preventive treatment in infants conducted between 2002 and 2004, antibody responses toPlasmodium falciparumblood-stage antigens in a cohort of 302 Mozambican children were evaluated by immunofluorescence antibody test and enzyme-linked immunosorbent assay at 5, 9, 12, and 24 months of age. We found that IgG subclass responses to EBA-175 were differentially associated with the incidence of malaria in the follow-up period. A double amount of cytophilic IgG1 or IgG3 was associated with a significant decrease in the incidence of malaria (incidence rate ratio [IRR] = 0.49, 95% confidence interval [CI] = 0.25 to 0.97, andP= 0.026 and IRR = 0.44, CI = 0.19 to 0.98, andP= 0.037, respectively), while a double amount of noncytophilic IgG4 was significantly correlated with an increased incidence of malaria (IRR = 3.07, CI = 1.08 to 8.78,P= 0.020). No significant associations between antibodies to the 19-kDa fragment of MSP-1 (MSP-119) or AMA-1 and incidence of malaria were found. Age, previous episodes of malaria, present infection, and neighborhood of residence were the main factors influencing levels of antibodies to all merozoite antigens. Deeper understanding of the acquisition of antibodies against vaccine target antigens in early infancy is crucial for the rational development and deployment of malaria control tools in this vulnerable population.

scholarly journals Differential Patterns of Human Immunoglobulin G Subclass Responses to Distinct Regions of a Single Protein, the Merozoite Surface Protein 1 of Plasmodium falciparum

2001 ◽  
Vol 69 (2) ◽  
pp. 1207-1211 ◽  
Author(s):  
David R. Cavanagh ◽  
Carlota Dobaño ◽  
Ibrahim M. Elhassan ◽  
Kevin Marsh ◽  
Ahmed Elhassan ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Immunoglobulin G ◽  
Malaria Infection ◽  
Surface Protein ◽  
Merozoite Surface Protein ◽  
Igg Subclass ◽  
Polymorphic Region ◽  
Merozoite Surface Protein 1 ◽  
Single Protein ◽  
Immunoglobulin G Subclass

ABSTRACT Comparisons of immunoglobulin G (IgG) subclass responses to the major polymorphic region and to a conserved region of MSP-1 in three cohorts of African villagers exposed to Plasmodium falciparum revealed that responses to Block 2 are predominantly IgG3 whereas antibodies to MSP-119 are mainly IgG1. The striking dominance of IgG3 to Block 2 may explain the short duration of this response and also the requirement for continuous stimulation by malaria infection to maintain clinical immunity.

scholarly journals Immunoglobulin G Subclass-Specific Responses against Plasmodium falciparum Merozoite Antigens Are Associated with Control of Parasitemia and Protection from Symptomatic Illness

2009 ◽  
Vol 77 (3) ◽  
pp. 1165-1174 ◽  
Author(s):  
Danielle I. Stanisic ◽  
Jack S. Richards ◽  
Fiona J. McCallum ◽  
Pascal Michon ◽  
Christopher L. King ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Immunoglobulin G ◽  
Vaccine Development ◽  
Surface Protein ◽  
Merozoite Surface Protein ◽  
Igg Subclass ◽  
Apical Membrane Antigen 1 ◽  
Falciparum Merozoite ◽  
Merozoite Antigens

ABSTRACT Substantial evidence indicates that antibodies to Plasmodium falciparum merozoite antigens play a role in protection from malaria, although the precise targets and mechanisms mediating immunity remain unclear. Different malaria antigens induce distinct immunoglobulin G (IgG) subclass responses, but the importance of different responses in protective immunity from malaria is not known and the factors determining subclass responses in vivo are poorly understood. We examined IgG and IgG subclass responses to the merozoite antigens MSP1-19 (the 19-kDa C-terminal region of merozoite surface protein 1), MSP2 (merozoite surface protein 2), and AMA-1 (apical membrane antigen 1), including different polymorphic variants of these antigens, in a longitudinal cohort of children in Papua New Guinea. IgG1 and IgG3 were the predominant subclasses of antibodies to each antigen, and all antibody responses increased in association with age and exposure without evidence of increasing polarization toward one subclass. The profiles of IgG subclasses differed somewhat for different alleles of MSP2 but not for different variants of AMA-1. Individuals did not appear to have a propensity to make a specific subclass response irrespective of the antigen. Instead, data suggest that subclass responses to each antigen are generated independently among individuals and that antigen properties, rather than host factors, are the major determinants of IgG subclass responses. High levels of AMA-1-specific IgG3 and MSP1-19-specific IgG1 were strongly predictive of a reduced risk of symptomatic malaria and high-density P. falciparum infections. However, no antibody response was significantly associated with protection from parasitization per se. Our findings have major implications for understanding human immunity and for malaria vaccine development and evaluation.

scholarly journals Genetic analysis of IgG subclass responses against RESA and MSP2 of Plasmodium falciparum in adults in Papua New Guinea

2000 ◽  
Vol 124 (1) ◽  
pp. 153-162 ◽  
Author(s):  
H. A. STIRNADEL ◽  
H.-P. BECK ◽  
M. P. ALPERS ◽  
T. A. SMITH
Keyword(s):  
Plasmodium Falciparum ◽  
Papua New Guinea ◽  
Vaccine Development ◽  
Familial Aggregation ◽  
Major Gene ◽  
Genetic Regulation ◽  
New Guinea ◽  
Variance Component Analysis ◽  
Igg Subclass ◽  
Host Genetic

Contributions of environmental and genetic factors to IgG subclass responses against Plasmodium falciparum antigens RESA and MSP2 were investigated among adults in a highly endemic area of Papua New Guinea. Heritabilities were estimated using variance component analysis. Familial aggregation of several responses was found, including IgG1, IgG2 and IgG3 responses against RESA, IgG1 and IgG3 responses against the 3D7 form of MSP2 and IgG1, IgG2 responses against the FC27 form of MSP2. Allowance for sharing of houses explained some of the non-genetic variance but not the familial aggregation. The variance of IgG3 responses against RESA and IgG1, IgG2 against MSP2 (FC27) was partly explained by sharing of HLA class II genotypes, although heritability was low. Segregation analyses indicated that any genetic regulation was more complex than governed by a single major gene. Such host genetic variation in responses to specific malaria antigens has implications for immuno-epidemiology and vaccine development.

scholarly journals IgG subclass responses to excreted-secreted antigens of Plasmodium falciparum in a low-transmission malaria area of the Peruvian Amazon

2018 ◽  
Vol 17 (1) ◽  
Author(s):  
Rafael Saavedra-Langer ◽  
Jorge Marapara ◽  
Andree Valle-Campos ◽  
Salomón Durand ◽  
Maria E. Vásquez-Chasnamote ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Igg Subclass ◽  
Peruvian Amazon ◽  
Low Transmission ◽  
Secreted Antigens

Significant Differences in FcγRIIa, FcγRIIa and FcγRIIIb Genes Polymorphism and Anti-malarial IgG Subclass Pattern are Associated with Severe Malaria in Saudi Children

2021 ◽  
Author(s):  
Amre Nasr ◽  
Ahmad Aljada ◽  
Osama Hamid ◽  
Hatim A. Elsheikh ◽  
Emad Masuadi ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Clinical Outcome ◽  
Severe Malaria ◽  
Malaria Infection ◽  
Pcr Amplification ◽  
Igg Subclass ◽  
Gene Variants ◽  
Disease Outcomes ◽  
Cellular And Humoral Immunity ◽  
Increased Susceptibility

Abstract Background: The FcyRs genotypes have been reported to play a key role in the defence against malaria parasites through both cellular and humoral immunity. This study aimed to investigate the possible correlation between FcγR (IIa, IIIa, and IIIb) genes polymorphism and the clinical outcome for anti‐malarial antibody response of Plasmodium falciparum infection among Saudi children. Material and methods: A 600 volunteers have been enrolled in this study, including 200 malaria-free control (MFC) subjects, 218 patients with uncomplicated malaria (UM) and 182 patients with severe malaria (SM). The FcγR genotypes was analysed using PCR amplification methods, and measurement of immunoglobulins were determine using ELISA. Results: The data revealed the FcγRIIa-R/R131 showed a statistically association with the increased susceptibility to SM when compared to UM patients. Furthermore, higher levels of IgG1, IgG2, and IgG4 were associated with the FcγRIIa-H/H131 genotypes among UM patients. Although the FcγRIIa-F/V176 genotype was not associated with UM, it showed a significant association with severe malaria. Interestingly, the FcγRIIa-V/V176 genotype was This study aimed to associated with protection against SM. Moreover, severe malaria patients carrying the FcγRIIa-F/F genotype showed higher levels of AMA-1-specific IgG2 and IgG4 antibodies. The FcγRIIIb NA1/NA1 and FcγRIIIb NA2/NA2 genotypes did not show significant differences between UM and the MFC. However, the genotype FcγRIIIb-NA2/NA2 was statistically associated with severe malaria. Conclusions: The data presented in this study strongly suggest the possible impact of FcyR (IIa, RIIIa and RIIIb) gene variants and anti-malaria IgG subclasses play a role in susceptibility to malaria infection and disease outcomes in Saudi children.

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