scholarly journals Lentiviral-Transduced Ectopic Expression of Androgenic Hormone in a Crustacean Hematopoietic Primary Cell Culture

2021 ◽  
Vol 8 ◽  
Author(s):  
Noa Rotem-Dai ◽  
Simy Weil ◽  
Yariv Greenshpan ◽  
Shai Abehsera ◽  
Rivka Manor ◽  
...  

Novel monosex biotechnologies in crustacean aquaculture involve the induction of sex reversal through manipulations of the androgenic gland (AG), and its most prominent factor, the insulin-like androgenic gland (IAG) hormone, during early developmental stages. In the giant freshwater prawn Macrobrachium rosenbergii, all-female populations are desirable for aquaculture, since the females can be stocked at higher densities and exhibit more uniform growth than males. Recently, a novel biotechnology for all-female aquaculture was developed based on injection into post-larvae of suspended primary AG cells from mature M. rosenbergii males. However, this biotechnology depends on the availability of appropriate male donors and it requires delicate surgical procedures on the small endocrine AG to produce appropriate quantities of cells for the sex manipulation. We therefore established a new platform for the production of M. rosenbergii IAG (Mr-IAG) in hematopoietic cells. The method rests on the induction of ectopic Mr-IAG expression under the white spot syndrome virus (WSSV) immediate-early 1 (IE1) promoter via a novel lentiviral vector. Our results demonstrate that M. rosenbergii primary cells infected with the Mr-IAG lentiviruses are capable of transcription, translation, and secretion of Mr-IAG in culture. Our new platform, which produces easy-to-harvest cells in abundance, could replace the AG cells used in the first step of the above-mentioned biotechnology for all-female aquaculture and, importantly, pave the way for producing monosex populations in other edible crustacean aquaculture species. In addition, a lentiviral system for crustacean cells provides a useful tool for basic and applied research in crustacean species.

2013 ◽  
Vol 193 ◽  
pp. 10-18 ◽  
Author(s):  
Tanapan Siangcham ◽  
Yotsawan Tinikul ◽  
Jaruwan Poljaroen ◽  
Morakot Sroyraya ◽  
Narin Changklungmoa ◽  
...  

2020 ◽  
Vol 21 (12) ◽  
pp. 4207
Author(s):  
Guang Yang ◽  
Zhijie Lu ◽  
Zhendong Qin ◽  
Lijuan Zhao ◽  
Gan Pan ◽  
...  

Giant freshwater prawns (Macrobrachium rosenbergii) are commonly found throughout the world. The size of the male giant freshwater prawn is much larger than that of the female. Therefore, understanding the molecular mechanism that underlies the sexual differentiation of M. rosenbergii is of both commercial and scientific importance. Insulin-like androgenic gland hormone (IAG) plays a key role in the differentiation of sex in M. rosenbergii. Although IAG has been investigated, the regulatory relationship between IAG and its binding protein partner, the insulin-like androgenic gland hormone-binding protein (IAGBP), has not been studied in M. rosenbergii. Here, we cloned and characterized the IAGBP from M. rosenbergii (Mr-IAGBP) for the very first time. Transcriptomic analysis showed that Mr-IAGBP mRNA was detected in a wide array of tissues with the highest expression found in the androgenic gland. The importance of IAG in male development was further demonstrated by an increase in IAG transcripts during the development of the androgenic gland and Mr-IAG was only highly transcribed in the androgenic gland of M. rosenbergii. Interestingly, we found that the Mr-IAG gene expression started during the 20th-day larva after hatching stage (LH20), followed (20th-day post-larval stage, PL20) by a gradual elevation of Mr-IAGBP levels. The levels of both genes peaked at the adult stage. The relationship between Mr-IAGBP and Mr-IAG was further analyzed using RNA interference. The injection of Mr-IAGBP double-stranded RNA (dsRNA) significantly reduced the transcription of Mr-IAG, while the amount of Mr-IAGBP mRNA and the translation of IAGBP protein was significantly reduced by the injection of Mr-IAG dsRNA. These results revealed that IAGBP is involved in IAG signaling. Furthermore, our data supports the hypothesis that (IAG and IAGBP)-IAG receptor signaling schemes exist in M. rosenbergii. Our results will provide important information for the further study of determining the sex of M. rosenbergii.


2011 ◽  
Vol 43 (3) ◽  
pp. 165-177 ◽  
Author(s):  
Ittipon Phoungpetchara ◽  
Yotsawan Tinikul ◽  
Jaruwan Poljaroen ◽  
Charoonroj Chotwiwatthanakun ◽  
Rapeepun Vanichviriyakit ◽  
...  

Heliyon ◽  
2021 ◽  
Vol 7 (1) ◽  
pp. e05898
Author(s):  
Tipsuda Thongbuakaew ◽  
Chanudporn Sumpownon ◽  
Attakorn Engsusophon ◽  
Napamanee Kornthong ◽  
Charoonroj Chotwiwatthanakun ◽  
...  

Chemosphere ◽  
2019 ◽  
Vol 222 ◽  
pp. 584-592 ◽  
Author(s):  
Yingying Zhang ◽  
Hang Zhuang ◽  
Hui Yang ◽  
Wen Xue ◽  
Liufu Wang ◽  
...  

2021 ◽  
Vol 214 ◽  
pp. 112067
Author(s):  
Qun Jiang ◽  
Ziyan Jiang ◽  
Shiqi Ao ◽  
Xiaojian Gao ◽  
Xinhai Zhu ◽  
...  

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