Insight into the Regulatory Relationships between the Insulin-Like Androgenic Gland Hormone Gene and the Insulin-Like Androgenic Gland Hormone-binding Protein Gene in Giant Freshwater Prawns (Macrobrachium rosenbergii)

Giant freshwater prawns (Macrobrachium rosenbergii) are commonly found throughout the world. The size of the male giant freshwater prawn is much larger than that of the female. Therefore, understanding the molecular mechanism that underlies the sexual differentiation of M. rosenbergii is of both commercial and scientific importance. Insulin-like androgenic gland hormone (IAG) plays a key role in the differentiation of sex in M. rosenbergii. Although IAG has been investigated, the regulatory relationship between IAG and its binding protein partner, the insulin-like androgenic gland hormone-binding protein (IAGBP), has not been studied in M. rosenbergii. Here, we cloned and characterized the IAGBP from M. rosenbergii (Mr-IAGBP) for the very first time. Transcriptomic analysis showed that Mr-IAGBP mRNA was detected in a wide array of tissues with the highest expression found in the androgenic gland. The importance of IAG in male development was further demonstrated by an increase in IAG transcripts during the development of the androgenic gland and Mr-IAG was only highly transcribed in the androgenic gland of M. rosenbergii. Interestingly, we found that the Mr-IAG gene expression started during the 20th-day larva after hatching stage (LH20), followed (20th-day post-larval stage, PL20) by a gradual elevation of Mr-IAGBP levels. The levels of both genes peaked at the adult stage. The relationship between Mr-IAGBP and Mr-IAG was further analyzed using RNA interference. The injection of Mr-IAGBP double-stranded RNA (dsRNA) significantly reduced the transcription of Mr-IAG, while the amount of Mr-IAGBP mRNA and the translation of IAGBP protein was significantly reduced by the injection of Mr-IAG dsRNA. These results revealed that IAGBP is involved in IAG signaling. Furthermore, our data supports the hypothesis that (IAG and IAGBP)-IAG receptor signaling schemes exist in M. rosenbergii. Our results will provide important information for the further study of determining the sex of M. rosenbergii.

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Glutamate related osmoregulation of guanine nucleotide-binding protein G (I) α2 from giant freshwater prawn (Macrobrachium rosenbergii) during molting and salinity stress

Aquaculture ◽

10.1016/j.aquaculture.2020.735000 ◽

2020 ◽

Vol 521 ◽

pp. 735000 ◽

Cited By ~ 3

Author(s):

Zhijie Lu ◽

Guang Yang ◽

Zhendong Qin ◽

Haiyang Shen ◽

Menglan Zhang ◽

...

Keyword(s):

Salinity Stress ◽

Binding Protein ◽

Macrobrachium Rosenbergii ◽

Nucleotide Binding ◽

Freshwater Prawn ◽

Protein G ◽

Guanine Nucleotide ◽

Guanine Nucleotide Binding Protein ◽

Giant Freshwater Prawn ◽

Guanine Nucleotide Binding

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The effects of serotonin, dopamine, gonadotropin-releasing hormones, and corazonin, on the androgenic gland of the giant freshwater prawn, Macrobrachium rosenbergii

General and Comparative Endocrinology ◽

10.1016/j.ygcen.2013.06.028 ◽

2013 ◽

Vol 193 ◽

pp. 10-18 ◽

Cited By ~ 25

Author(s):

Tanapan Siangcham ◽

Yotsawan Tinikul ◽

Jaruwan Poljaroen ◽

Morakot Sroyraya ◽

Narin Changklungmoa ◽

...

Keyword(s):

Macrobrachium Rosenbergii ◽

Freshwater Prawn ◽

Androgenic Gland ◽

Giant Freshwater Prawn

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Evaluation of claw development in giant freshwater prawn, Macrobrachium rosenbergii (de Man, 1879)

Indian Journal of Fisheries ◽

10.21077/ijf.2018.65.1.61139-19 ◽

2018 ◽

Vol 65 (1) ◽

Author(s):

P. P. Suresh Babu ◽

P. Srinivasa Rao ◽

I. F. Biju ◽

C. Dharmadurai ◽

A. M. Babitha Rani

Keyword(s):

Body Weight ◽

Macrobrachium Rosenbergii ◽

The Body ◽

Freshwater Prawn ◽

Weight Class ◽

Class Interval ◽

Giant Freshwater Prawn ◽

Males And Females ◽

De Man ◽

The Relationship

Dynamics of claw development in Macrobrachium rosenbergii (de Man, 1879) was evaluated through monosex culture. The segregated males and females were stocked separately in two earthen ponds of 200 m2 area, at a density of 2.5 m-2 and reared for 3 months. Percentage contribution of claw weight to body weight (PCB) increased from 8.96 to 14.4% in the first month, but the change was minimal (14.4 to 17.19%) during the rest of the culture period. In order to delineate the relationship further, the data obtained was pooled together and classified into different classes based on the body weight (class interval 10 g). Interestingly, PCB in males decreased gradually upto 30 - 40 g weight class and then increased considerably for higher weight classes. But for females, the increase in PCB was marginal.

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The Regulatory Relationships Between the Gonad-Inhibiting Hormone and Insulin-Like Androgenic Gland Hormone-Binding Protein Genes in the Eyestalk-Androgenic Gland-Testis Axis of Macrobrachium rosenbergii

Frontiers in Marine Science ◽

10.3389/fmars.2021.775191 ◽

2021 ◽

Vol 8 ◽

Author(s):

Meizhen Tang ◽

Zhijie Lu ◽

Sarath V Babu ◽

Guang Yang ◽

Yanan Li ◽

...

Keyword(s):

Macrobrachium Rosenbergii ◽

Inhibitory Factor ◽

Androgenic Gland ◽

Hormone Binding ◽

Eyestalk Ablation ◽

Expression Levels ◽

Protein Levels ◽

Sexual Regulation ◽

Hormone Binding Protein ◽

Gonad Inhibiting Hormone

Gonad-inhibiting hormone (GIH) belongs to a family of neuropeptides that are released from the eyestalks of male crustaceans and plays key roles in gonadal maturity, reproduction, and molting. However, the detailed mechanisms underlying the effects of GIH on sexual regulation have yet to be elucidated. In the present study, we aimed to demonstrate how GIH mediate the activity of the androgenic gland (AG) to affect sexual regulation. To do this, we cloned and characterized a GIH sequence from Macrobrachium rosenbergii (MrGIH). The open reading frame (ORF) of MrGIH was 360 bp and codes for a polypeptide of 119 amino acids and a putative protein of 13.56 KDa. Tissue analysis showed that MrGIH is widely expressed in a range of tissues but particularly, the eyestalk, intestine, and nerve cord. Following the dsRNA silencing of MrGIH for 24 h, the expression levels of MrGIH were down-regulated in both the eyestalk and AG when compared with the negative control, but significantly increased the expression of Macrobrachium rosenbergii insulin-like androgenic gland hormone-binding protein (MrIAGBP) in AG, thus suggesting that MrGIH is an inhibitory factor for MrIAGBP. In addition, we found that eyestalk removal on certain days led to increased expression levels of MrIAGBP expression. The expression levels of MrIAGBP peaked at 2 d in the AG after unilateral and bilateral eyestalk ablation, exhibiting a 7.27- and 6.03-fold increase, respectively. Afterward, the expression of GIH protein levels were down-regulated and IAGBP protein levels were up-regulated after GIH silencing using immunohistochemistry method, combined with the increase of IAGBP protein levels after eyestalk ablation, confirming that MrGIH is an inhibitory factor that can moderately regulate AG development and IAGBP expression. Collectively, our findings enriched the mechanisms that control the sexual regulation pathway of male M. rosenbergii, and provided significant information for further explorations of the mechanism underlying sex regulation in other decapod crustaceans.

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Insulin sensitivity and secretion influence the relationship between growth hormone-binding-protein and leptin

Clinical Endocrinology ◽

10.1046/j.1365-2265.2000.00907.x ◽

2000 ◽

Vol 52 (2) ◽

pp. 159-164 ◽

Cited By ~ 12

Author(s):

José-Manuel Fernandez-Real ◽

Maria Luisa Granada ◽

Ana Ruzafa ◽

Roser Casamitjana ◽

Wifredo Ricart

Keyword(s):

Growth Hormone ◽

Insulin Sensitivity ◽

Binding Protein ◽

Hormone Binding ◽

Growth Hormone Binding Protein ◽

Hormone Binding Protein ◽

The Relationship

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Lentiviral-Transduced Ectopic Expression of Androgenic Hormone in a Crustacean Hematopoietic Primary Cell Culture

Frontiers in Marine Science ◽

10.3389/fmars.2021.677679 ◽

2021 ◽

Vol 8 ◽

Author(s):

Noa Rotem-Dai ◽

Simy Weil ◽

Yariv Greenshpan ◽

Shai Abehsera ◽

Rivka Manor ◽

...

Keyword(s):

Developmental Stages ◽

Lentiviral Vector ◽

Ectopic Expression ◽

Macrobrachium Rosenbergii ◽

Freshwater Prawn ◽

Androgenic Gland ◽

Giant Freshwater Prawn ◽

Androgenic Hormone ◽

Basic And Applied Research ◽

Early Developmental

Novel monosex biotechnologies in crustacean aquaculture involve the induction of sex reversal through manipulations of the androgenic gland (AG), and its most prominent factor, the insulin-like androgenic gland (IAG) hormone, during early developmental stages. In the giant freshwater prawn Macrobrachium rosenbergii, all-female populations are desirable for aquaculture, since the females can be stocked at higher densities and exhibit more uniform growth than males. Recently, a novel biotechnology for all-female aquaculture was developed based on injection into post-larvae of suspended primary AG cells from mature M. rosenbergii males. However, this biotechnology depends on the availability of appropriate male donors and it requires delicate surgical procedures on the small endocrine AG to produce appropriate quantities of cells for the sex manipulation. We therefore established a new platform for the production of M. rosenbergii IAG (Mr-IAG) in hematopoietic cells. The method rests on the induction of ectopic Mr-IAG expression under the white spot syndrome virus (WSSV) immediate-early 1 (IE1) promoter via a novel lentiviral vector. Our results demonstrate that M. rosenbergii primary cells infected with the Mr-IAG lentiviruses are capable of transcription, translation, and secretion of Mr-IAG in culture. Our new platform, which produces easy-to-harvest cells in abundance, could replace the AG cells used in the first step of the above-mentioned biotechnology for all-female aquaculture and, importantly, pave the way for producing monosex populations in other edible crustacean aquaculture species. In addition, a lentiviral system for crustacean cells provides a useful tool for basic and applied research in crustacean species.

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