basic and applied research
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HortScience ◽  
2022 ◽  
Vol 57 (2) ◽  
pp. 247-256
Author(s):  
Cary A. Mitchell

The most recent platform for protected horticultural crop production, with the shortest history to date, is located entirely indoors, lacking even the benefit of free, natural sunlight. Although this may not sound offhand like a good idea for commercial specialty-crop production, the concept of indoor controlled-environment plant growth started originally for the benefit of researchers—to systematically investigate effects of specific environmental factors on plant growth and development in isolation from environmental factors varying in uncontrolled ways that would confound or change experimental findings. In addition to its value for basic and applied research, it soon was discovered that providing nonlimiting plant-growth environments greatly enhanced crop yield and enabled manipulation of plant development in ways that were never previously possible. As supporting technology for indoor crop production has improved in capability and efficiency, energy requirements have declined substantially for growing crops through entire production cycles in completely controlled environments, and this combination has spawned a new sector of the controlled-environment crop-production industry. This article chronicles the evolution of events, enabling technologies, and entrepreneurial efforts that have brought local, year-round indoor crop production to the forefront of public visibility and the threshold of profitability for a growing number of specialty crops in locations with seasonal climates.


Check List ◽  
2022 ◽  
Vol 18 (1) ◽  
pp. 67-77
Author(s):  
Rebeca Casique-Valdés ◽  
Sten Anslan ◽  
Fernando Galindo-García ◽  
Sergio R. Sanchez-Peña

We report fungal pathogens of invertebrates (FPI) (Entomophthorales, Hypocreales, and Orbiliales) from roots of an endemic Mexican pine, Pinus greggii Engelm. ex Parl., at four primary montane forests using next-generation sequencing. We found twenty-nine OTUs from 18 genera of FPI associated to the roots of P. greggii. New records for Mexico are: Beauveria felina (DC.) J.W.Carmich., Dactylella mammillata S.M. Dixon, Dactylella ramosa Matsushima, Drechslerella brochopaga (Drechsler) M. Scholler, Hagedorn & A. Rubner, Hirsutella minnesotensis Chen, Liu & Chen, Leptobacillium leptobactrum (W.Gams) Zare & W.Gams, Metapochonia variabilis Z.F.Zhang, F.Liu & L.Cai, Monacrosporium leptosporum (Drechsler) A. Rubner, and Simplicillium aogashimaense Nonaka, Kaifuchi & Masuma. A largely unknown array of fungal pathogens of invertebrates are likely to be found in Mexican forests.  This work facilitates future analyses of fungal diversity in these primary forests, as well as basic and applied research in biological control.


2022 ◽  
Vol 0 (0) ◽  
Author(s):  
Maya Madhavan ◽  
Sabeena Mustafa

Abstract Life science is the study of living organisms, including bacteria, plants, and animals. Given the importance of biology, chemistry, and bioinformatics, we anticipate that this chapter may contribute to a better understanding of the interdisciplinary connections in life science. Research in applied biological sciences has changed the paradigm of basic and applied research. Biology is the study of life and living organisms, whereas science is a dynamic subject that as a result of constant research, new fields are constantly emerging. Some fields come and go, whereas others develop into new, well-recognized entities. Chemistry is the study of composition of matter and its properties, how the substances merge or separate and also how substances interact with energy. Advances in biology and chemistry provide another means to understand the biological system using many interdisciplinary approaches. Bioinformatics is a multidisciplinary or rather transdisciplinary field that encourages the use of computer tools and methodologies for qualitative and quantitative analysis. There are many instances where two fields, biology and chemistry have intersection. In this chapter, we explain how current knowledge in biology, chemistry, and bioinformatics, as well as its various interdisciplinary domains are merged into life sciences and its applications in biological research.


2022 ◽  
Vol 17 (1) ◽  
pp. 1-23
Author(s):  
Levente Alpek B. ◽  
◽  
Róbert Tésits ◽  
Melissza Zita Lempek ◽  
Péter Kókay ◽  
...  

The scholarly journal Modern Geográfia has been providing a publication platform for researchers of geography and, more broadly, the natural and social sciences for 15 years now. Effective dissemination of scientific results is of paramount importance in broadening the horizons of both basic and applied research. The aim of the present study is to explore the role of Modern Geográfia in the above process, thus to outline its profile and possible directions for its further development. The applied method can be an effective help in formulating the portfolio and the strategic goals of other scholarly journals. The methodological basis of the research is provided by the statistical analysis of the journal based on a primary database containing several indicators. The results cover the number of articles published in the journal, in addition to the dynamics of the publications, its profiling, the presentation of the affected topics, and the evaluation of the trends of recent years. In addition to all this, the study reviews the team of professionals that has given content to the journal with the results of its scientific research.


Author(s):  
Pengfei Wang ◽  
Chunguo Liu ◽  
Shida Wang ◽  
Lili Wen ◽  
Zhibin Shi ◽  
...  

AbstractAfrican swine fever (ASF) is an acute hemorrhagic disease of domestic pigs. The causative agent of ASF, ASF virus (ASFV), is a double-stranded DNA virus, the sole member in the family Asfarviridae. The non-structural protein pB602L of ASFV is a molecular chaperone of the major capsid protein p72 and plays a key role in icosahedral capsid assembly. This protein is antigenic and is a target for developing diagnostic tools for ASF. To generate monoclonal antibodies (mAbs) against pB602L, a prokaryotically expressed recombinant pB602L protein was produced, purified, and used as an antigen to immunize mice. A total of eight mouse mAbs were obtained, and their binding epitopes were screened by Western blot using an overlapping set of polypeptides from pB602L. Three linear epitopes were identified and designated epitope 1 (366ANRERYNY373), epitope 2 (415GPDAPGLSI423), and epitope 3 (498EMLNVPDD505). Based on the epitope recognized, the eight mAbs were placed into three groups: group 1 (B2A1, B2F1, and B2D10), group 2 (B2H10, B2B2, B2D8, and B2A3), and group 3 (B2E12). The mAbs B2A1, B2H10, and B2E12, each representing one of the groups, were used to detect pB602L in ASFV-infected porcine alveolar macrophages (PAMs) and pig tissues, using an indirect fluorescence assay (IFA) and immunohistochemical staining, respectively. The results showed that pB602L was detectable with all three mAbs in immunohistochemical staining, but only B2H10 was suitable for detecting the proteins in ASFV-infected PAMs by IFA. In summary, we developed eight anti-pB602L mouse mAbs recognizing three linear epitopes in the protein, which can be used as reagents for basic and applied research on ASFV.


2022 ◽  
Vol 82 ◽  
Author(s):  
W. A. Chiba de Castro ◽  
R. C. Luz ◽  
C. K. Peres

Abstract As a result of biodiversity and ecosystem service losses associated with biological invasions, there has been growing interest in basic and applied research on invasive species aiming to improve management strategies. Tradescantia zebrina is a herbaceous species increasingly reported as invasive in the understory of disturbed forest ecosystems. In this study, we assess the effect of spatial and seasonal variation on biological attributes of this species in the Atlantic Forest. To this end, we measured attributes of T. zebrina associated with plant growth and stress in the four seasons at the forest edge and in the forest interior of invaded sites in the Iguaçu National Park, Southern Brazil. The invasive plant had higher growth at the forest edge than in the forest interior and lower leaf asymmetry and herbivory in the winter than in the summer. Our findings suggest that the forest edge environment favours the growth of T. zebrina. This invasive species is highly competitive in the understory of semi-deciduous seasonal forests all over the year. Our study contributes to the management of T. zebrina by showing that the summer is the best season for controlling this species.


2021 ◽  
pp. 160-167
Author(s):  
A. TSINA

The article presents generalizations, in line with world and domestic educational processes, ideas and forms of development of scientific and pedagogical school “Aesthetic culture in the preparation of future educators” by Professor V. Tytarenko. The method of historical-retrospective analysis revealed the progressive achievements of the scientific school as a research center and a collective form of scientific creativity in the field of technological education on theoretical and methodological aspects of technological education of students by means of aesthetic culture and design. The study of the laws of formation of aesthetic culture of pupils and students by means of Ukrainian folk crafts began with the search and study of samples of Ukrainian folk embroidery, description of its techniques, distribution in different areas of certain ornaments, colors, traditions. The result of this work were her books “Ukrainian towel as a means of national education and a work of folk decorative and applied arts”, “Poltava traditional embroidery: past and present”. V. Tytarenko is one of the first initiators of conducting comprehensive research and experiments in Ukraine in the field of decorative and applied art, as well as the methodology of scientific and pedagogical research in this field of knowledge. The scientific interests of Valentyna Tytarenko’s scientific school are focused on the study of the laws of formation of the aesthetic culture of pupils and students by means of Ukrainian folk crafts. The creative team of like-minded people has successfully carried out research in recent years, funded by the state budget on “Theory and methods of forming the aesthetic culture of future technology teachers in the process of personality-oriented training”, and today completes research on “Theoretical -methodical aspects of technological education of pupils and students by means of culture and design”. Continuation of basic and applied research on this topic is implemented in the Poltava regional special research group of teachers of labor training and technology “Design as a method of cognition in the educational field of technology”, which works under the leadership of Professor V. Tytarenko. The formulated results of its activity on theoretical and methodological aspects of technological education of pupils and students by means of aesthetic culture and design which we can observe as a result of opening of ways of use of the maintenance and a technique of preparation of future teachers of labor training and student’s youth for training of the Ukrainian national crafts confirm their full conformity. data presented in the materials of dissertation research of representatives of the scientific school.


2021 ◽  
Vol 23 (1) ◽  
pp. 205
Author(s):  
Tomasz J. Wasowicz

The interactions of ions with molecules and the determination of their dissociation patterns are challenging endeavors of fundamental importance for theoretical and experimental science. In particular, the investigations on bond-breaking and new bond-forming processes triggered by the ionic impact may shed light on the stellar wind interaction with interstellar media, ionic beam irradiations of the living cells, ion-track nanotechnology, radiation hardness analysis of materials, and focused ion beam etching, deposition, and lithography. Due to its vital role in the natural environment, the pyridine molecule has become the subject of both basic and applied research in recent years. Therefore, dissociation of the gas phase pyridine (C5H5N) into neutral excited atomic and molecular fragments following protons (H+) and dihydrogen cations (H2+) impact has been investigated experimentally in the 5–1000 eV energy range. The collision-induced emission spectroscopy has been exploited to detect luminescence in the wavelength range from 190 to 520 nm at the different kinetic energies of both cations. High-resolution optical fragmentation spectra reveal emission bands due to the CH(A2Δ → X2Πr; B2Σ+ → X2Πr; C2Σ+ → X2Πr) and CN(B2Σ+ → X2Σ+) transitions as well as atomic H and C lines. Their spectral line shapes and qualitative band intensities are examined in detail. The analysis shows that the H2+ irradiation enhances pyridine ring fragmentation and creates various fragments more pronounced than H+ cations. The plausible collisional processes and fragmentation pathways leading to the identified products are discussed and compared with the latest results obtained in cation-induced fragmentation of pyridine.


2021 ◽  
Vol 8 ◽  
Author(s):  
Juan Jovel ◽  
Russell Greiner

Machine learning (ML) approaches are a collection of algorithms that attempt to extract patterns from data and to associate such patterns with discrete classes of samples in the data—e.g., given a series of features describing persons, a ML model predicts whether a person is diseased or healthy, or given features of animals, it predicts weather an animal is treated or control, or whether molecules have the potential to interact or not, etc. ML approaches can also find such patterns in an agnostic manner, i.e., without having information about the classes. Respectively, those methods are referred to as supervised and unsupervised ML. A third type of ML is reinforcement learning, which attempts to find a sequence of actions that contribute to achieving a specific goal. All of these methods are becoming increasingly popular in biomedical research in quite diverse areas including drug design, stratification of patients, medical images analysis, molecular interactions, prediction of therapy outcomes and many more. We describe several supervised and unsupervised ML techniques, and illustrate a series of prototypical examples using state-of-the-art computational approaches. Given the complexity of reinforcement learning, it is not discussed in detail here, instead, interested readers are referred to excellent reviews on that topic. We focus on concepts rather than procedures, as our goal is to attract the attention of researchers in biomedicine toward the plethora of powerful ML methods and their potential to leverage basic and applied research programs.


Author(s):  
Carlos Renato Zacharias

2013 is going to be an interesting year for the High Dilution research field. There is a tendency to revise basic concepts and common beliefs, while refining models and theories. The fundamental research team seems to be open to deeper discussions, aiming to reinforce some hypothesis and discharge others. In somehow, we are stuck in a vicious way of think, trying to manage and describe an informational phenomenon into a molecular framework. The challenge offered to those researchers involved with basic science is to figure out how to deal with such phenomenon, since previous theories and models have not shown to be matched! In the other hand, there is a tendency to explore the HD phenomenon in its technological branch. While basic research is important to describe how things work, the applied research has a more intense social and economic appeal. To develop technology based on HD means to give a different kind of visibility to the field, attracting sponsors, companies, entrepreneurs, always committed to the market regulatory laws. I am expecting for this year some important movements, both in the basic and applied research. Of course, it will be only a new beginning, as these changes require a lot of work, discussions and insights. But this transition time is very welcome!


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