scholarly journals Involvement of Heme in Colony Spreading of Staphylococcus aureus

2020 ◽  
Vol 11 ◽  
Author(s):  
Chao-Chin Liu ◽  
Mei-Hui Lin
Keyword(s):  
Staphylococcus Aureus ◽  
Colony Spreading

Sugar-responsive gene expression and the agr system are required for colony spreading in Staphylococcus aureus

2011 ◽  
Vol 51 (3) ◽  
pp. 178-185 ◽  
Author(s):  
Tomofumi Ueda ◽  
Chikara Kaito ◽  
Yosuke Omae ◽  
Kazuhisa Sekimizu
Keyword(s):  
Gene Expression ◽  
Staphylococcus Aureus ◽  
Colony Spreading ◽  
Responsive Gene

scholarly journals Colony Spreading in Staphylococcus aureus

2006 ◽  
Vol 189 (6) ◽  
pp. 2553-2557 ◽  
Author(s):  
Chikara Kaito ◽  
Kazuhisa Sekimizu
Keyword(s):  
Staphylococcus Aureus ◽  
Soft Agar ◽  
Clinical Isolates ◽  
Wild Type ◽  
Teichoic Acids ◽  
Colony Spreading ◽  
Different Strains ◽  
Wall Teichoic Acids

ABSTRACT Wild-type Staphylococcus aureus rapidly expands on the surface of soft agar plates. The rates of expansion and the shapes of the resultant giant colonies were distinct for different strains of laboratory stocks and clinical isolates. The colony spreading abilities did not correlate with the biofilm-forming abilities in these strains. Insertional disruption of the dltABCD operon, which functions at the step of d-alanine addition to teichoic acids, and of the tagO gene, which is responsible for the synthesis of wall teichoic acids, decreased the colony spreading ability. The results indicate that wall teichoic acids and d-alanylation of teichoic acids are required for colony spreading.

scholarly journals Cell-Surface Phenol Soluble Modulins Regulate Staphylococcus aureus Colony Spreading

PLoS ONE ◽  
2016 ◽  
Vol 11 (10) ◽  
pp. e0164523 ◽  
Author(s):  
Hayato Kizaki ◽  
Yosuke Omae ◽  
Fumiaki Tabuchi ◽  
Yuki Saito ◽  
Kazuhisa Sekimizu ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Cell Surface ◽  
Colony Spreading

scholarly journals The Sortase A Substrates FnbpA, FnbpB, ClfA and ClfB Antagonize Colony Spreading of Staphylococcus aureus

PLoS ONE ◽  
2012 ◽  
Vol 7 (9) ◽  
pp. e44646 ◽  
Author(s):  
Eleni Tsompanidou ◽  
Emma L. Denham ◽  
Mark J. J. B. Sibbald ◽  
Xiao-mei Yang ◽  
Jolien Seinen ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Sortase A ◽  
Colony Spreading

scholarly journals A Novel Gene, fudoh, in the SCCmec Region Suppresses the Colony Spreading Ability and Virulence of Staphylococcus aureus

PLoS ONE ◽  
2008 ◽  
Vol 3 (12) ◽  
pp. e3921 ◽  
Author(s):  
Chikara Kaito ◽  
Yosuke Omae ◽  
Yasuhiko Matsumoto ◽  
Makiko Nagata ◽  
Hiroki Yamaguchi ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Novel Gene ◽  
Colony Spreading

scholarly journals Identification of Staphylococcus aureus Colony-Spreading Stimulatory Factors from Mammalian Serum

PLoS ONE ◽  
2014 ◽  
Vol 9 (5) ◽  
pp. e97670 ◽  
Author(s):  
Yosuke Omae ◽  
Kazuhisa Sekimizu ◽  
Chikara Kaito
Keyword(s):  
Staphylococcus Aureus ◽  
Colony Spreading

scholarly journals Requirement of the agr Locus for Colony Spreading of Staphylococcus aureus

2010 ◽  
Vol 193 (5) ◽  
pp. 1267-1272 ◽  
Author(s):  
E. Tsompanidou ◽  
M. J. J. B. Sibbald ◽  
M. A. Chlebowicz ◽  
A. Dreisbach ◽  
J. W. Back ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Colony Spreading

Cytochemical Studies of Cell Wall Biosynthesis in Staphylococcus Aureus

1972 ◽  
Vol 30 ◽  
pp. 328-329
Author(s):  
Masaatsu Koike ◽  
Koichi Nakashima ◽  
Kyoko Iida
Keyword(s):  
Staphylococcus Aureus ◽  
Periodate Oxidation ◽  
Early Time ◽  
The Other ◽  
Penicillin G ◽  
Cell Wall Biosynthesis ◽  
Septal Wall ◽  
Peptidoglycan Biosynthesis ◽  
Gram Positive Bacteria ◽  
Cross Linkage

Penicillin exerts the activity to inhibit the peptide cross linkage between each polysaccharide backbone at the final stage of wall-peptidoglycan biosynthesis of bacteria. Morphologically, alterations of the septal wall and mesosome in gram-positive bacteria, which were occurred in early time after treatment with penicillin, have been observed. In this experiment, these alterations were cytochemically investigated by means of silver-methenamine staining after periodate oxidation, which is applied for detection of localization of wall mucopolysaccharide.Staphylococcus aureus strain 209P treated with 100 u/ml of penicillin G was divided into two aliquotes. One was fixed by Kellenberger-Ryter's OSO4 fixative at 30, 60 and 120 min after addition of the antibiotic, dehydrated through alcohol series, and embedded in Epon 812 (Specimen A). The other was fixed by 21 glutaraldehyde, dehydrated through glycolmethacrylate series and embedded in glycolmethacrylate mixture, according to Bernhard's method (Specimen B).

Gold labeling of teichoic acid on adjacent surfaces of staphylococcus aureus

1990 ◽  
Vol 48 (3) ◽  
pp. 578-579
Author(s):  
Margaret Hukee
Keyword(s):  
Staphylococcus Aureus ◽  
Teichoic Acid ◽  
Protein Labeling ◽  
Minimal Amount ◽  
Section Thickness ◽  
Double Labeling ◽  
Parallel Surfaces ◽  
Supporting Membrane

Gold labeling of two antigens (double labeling) is often done on two section surfaces separated by section thickness. Whether labeling is done on both sides of the same section or on two parallel surfaces separated by section thickness (PSSST), comparable results are dependent on an equal number of epitopes being exposed at each surface. We propose a method to study protein labeling within the same field of proteins, by examining two directly adjacent surfaces that were split during sectioning. The number of labeling sites on adjacent surfaces (AS) were compared to sites on PSSST surfaces in individual bacteria.Since each bacteria needed to be recognizable in all three section surfaces, one-hole grids were used for labeling. One-hole grids require a supporting membrane and excessive handling during labeling often ruptures the membrane. To minimize handling, a labeling chamber was designed that is inexpensive, disposable, minimizes contamination, and uses a minimal amount of solution.

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