scholarly journals Low Doses of Sucralose Alter Fecal Microbiota in High-Fat Diet-Induced Obese Rats

2021 ◽  
Vol 8 ◽  
Author(s):  
Minchun Zhang ◽  
Jie Chen ◽  
Minglan Yang ◽  
Cheng Qian ◽  
Yu Liu ◽  
...  
Keyword(s):  
Ribosomal Rna ◽  
High Fat Diet ◽  
Fecal Microbiota ◽  
Calorie Intake ◽  
Sprague Dawley ◽  
Functional Prediction ◽  
High Fat ◽  
Sprague Dawley Rats ◽  
Obese Rats ◽  
High Doses

Artificial sweeteners (AS) have been widely used as sugar substitutes to reduce calorie intake. However, it was reported that high doses of AS induced glucose intolerance via modulating gut microbiota. The objective of this study was to investigate the effects of lower doses of sucralose on fecal microbiota in obesity. Eight weeks after high-fat diet (HFD), the male Sprague Dawley rats were randomly divided into four groups (6 in each group) and administrated by a daily gavage of 2 ml normal saline (CON), 0.54 mM sucralose (N054), 0.78 mM sucralose (N078), and 324 mM sucrose (S324), respectively. After 4 weeks, fecal samples were obtained and analyzed by 16S ribosomal RNA gene sequencing. The richness and diversity of fecal microbiota were not changed by sucralose or sucrose. Both 0.54 mM (0.43 mg) and 0.78 mM (0.62 mg) sucralose tended to reduce the beneficial bacteria, Lactobacillaceae and Akkermansiaceae. The relative abundance of family Acidaminoccaceae and its genus Phascolarctobacteriam were increased after 0.54 mM sucralose. In functional prediction, 0.54 mM sucralose increased profiles of carbohydrate metabolism, whereas 0.78 mM sucralose enhanced those of amino acid metabolism. The lower doses of sucralose might alter the compositions of fecal microbiota. The effects of sucralose in different dosages should be considered in the future study.

scholarly journals Low-doses of Sucralose Alters Fecal Microbiota in High-fat Diet Induced Obese Rats

2021 ◽  
Author(s):  
Minchun Zhang ◽  
Jie Chen ◽  
Minglan Yang ◽  
Cheng Qian ◽  
Yu Liu ◽  
...  
Keyword(s):  
Ribosomal Rna ◽  
High Fat Diet ◽  
Fecal Microbiota ◽  
Calorie Intake ◽  
Sprague Dawley ◽  
High Fat ◽  
Metabolic Functions ◽  
Sprague Dawley Rats ◽  
Obese Rats ◽  
High Doses

Abstract Background: Artificial sweeteners (AS) are widely used as sugar substitutes to reduce calorie intake. However, high doses of AS induced glucose tolerance by modulating gut microbiota. The objective of this study was to investigate the effects of lower doses of sucralose on fecal microbiota in obesity. Methods: Eight weeks after high-fat diet, the male Sprague Dawley rats were randomly divided into four groups (n=24) and administrated by a daily gavage of 2ml normal saline (CON), 0.54mM sucralose (N054), 0.78mM sucralose (N078) and 324mM sucrose (S324) respectively. After four weeks, fecal samples were obtained and used in 16S ribosomal RNA gene sequencing. Results: The richness and diversity of fecal microbiota did not change by these sucralose and sucrose dosage. Both 0.54mM (0.43mg) and 0.78mM (0.62mg) tended to reduce the beneficial bacteria, Lactobacillaceae and Akkermansiaceae. The relative abundance of family Acidaminoccaceae and its genus Phascolarctobacteriam were increased with 0.54mM sucralose. In functional prediction, 0.54mM sucralose increased profiles of carbohydrate metabolism, whereas 0.78 mM sucralose enhanced that of amino acids metabolism. Conclusions: The lower doses of sucralose altered the compositions and the metabolic functions of fecal microbiota. The benefits of sucralose and its recommended dose for obese patients should be reassessed comprehensively.

scholarly journals Anthocyanins from black wolfberry (Lycium ruthenicum Murr.) prevent inflammation and increase fecal fatty acid in diet-induced obese rats

RSC Advances ◽  
2017 ◽  
Vol 7 (75) ◽  
pp. 47848-47853 ◽  
Author(s):  
Jinjin Yin ◽  
Tao Wu
Keyword(s):  
Oxidative Stress ◽  
Fatty Acid ◽  
High Fat Diet ◽  
Sprague Dawley ◽  
High Fat ◽  
Sprague Dawley Rats ◽  
Lycium Ruthenicum ◽  
Obese Rats ◽  
Lycium Ruthenicum Murr ◽  
Obese Male

This study aimed to determine whether black wolfberry (Lycium ruthenicumMurr.) anthocyanin (BWA) consumption can alleviate oxidative stress and reduce inflammation in high-fat diet-induced obese male Sprague-Dawley rats.

Effects of Exposure of Obese Rats to Simulated High Altitudes

1957 ◽  
Vol 191 (2) ◽  
pp. 371-376 ◽  
Author(s):  
Paul D. Altland ◽  
Olaf Mickelsen ◽  
Benjamin Highman
Keyword(s):  
High Fat Diet ◽  
Slow Rate ◽  
Heart Weight ◽  
Simulated Altitude ◽  
Sprague Dawley ◽  
High Fat ◽  
Altitude Exposure ◽  
Sprague Dawley Rats ◽  
Obese Rats ◽  
The Mean

Osborne-Mendel rats became obese when fed a high fat diet for 6–15 months. Obese rats and stock-fed rats were exposed 6 1/2 hours a day, 5 days a week to 18,000 or 25,000 feet simulated altitude for from 4 to 6 months. Both groups of rats developed the same degree of polycythemia and had the same mortality rate. The obese rats lost more weight from altitude exposure and also showed a slightly higher incidence of cardiovalvular thickening and cardiac vegetations, particularly among those that died. The mean heart weight of unexposed obese rats was 50% greater than that of stock-fed rats; the heart weights of both groups increased about 70% following 191 days of exposure. In acute altitude tests at 33,500 feet simulated altitude, both Osborne-Mendel and Sprague-Dawley rats on the high-fat diet, irrespective of the degree of obesity, died within 86 minutes. Sixty per cent of the stock-fed rats survived for a longer period. Some of the heaviest Osborne-Mendel rats on the high-fat diet died within 3 minutes at this altitude. Preoxygenation prevented these early deaths, but neither preoxygenation nor a slow rate of ascent (500 ft/min) had any effect on the high mortality of rats on a high-fat diet exposed to altitude.

The Effects of Maternal High Fat Diet on the Lingual CD36 Lipid Sensors of the Offspring Sprague Dawley Rats

Metabolism ◽  
2021 ◽  
Vol 116 ◽  
pp. 154497
Author(s):  
Elif Günalan ◽  
Meyli Ezgi Karagöz ◽  
Bayram Yılmaz ◽  
Burcu Gemici
Keyword(s):  
High Fat Diet ◽  
Sprague Dawley ◽  
High Fat ◽  
Sprague Dawley Rats ◽  
Lipid Sensors

Abstract 382: Anti-MAA Antibodies in Sprague Dawley Rats are Increased in Response to a High Fat Western Diet

2013 ◽  
Vol 33 (suppl_1) ◽  
Author(s):  
Michael J Duryee ◽  
Anand Dusad ◽  
Scott W Shurmur ◽  
Michael D Johnston ◽  
Robert P Garvin ◽  
...  
Keyword(s):  
High Fat Diet ◽  
Normal Diet ◽  
Western Diet ◽  
Sprague Dawley ◽  
High Fat ◽  
Sprague Dawley Rats ◽  
Stable Plaque ◽  
Circulating Antibodies ◽  
Modified Proteins ◽  
Progression Of Atherosclerosis

Introduction Malondialdehyde/Acetaldehyde (MAA) modified proteins have been suggested to play a role in the development/progression of atherosclerosis. Circulating antibodies directed against these proteins have recently been shown to be associated with the severity of the disease. More specifically, the isotype of the antibody to MAA correlated with either an acute MI (IgG) or stable plaque formation (IgA) formation. MAA is thought to form as a result of the oxidation of fat(s) and thus the concentration and antibody response should reflect the amount of fat in the diet. Objective The purpose of this study was to evaluate the antibody responses to MAA modified proteins following immunization and high fat western diet feeding in rats. Methods Male Sprague Dawley rats were immunized with MAA-modified protein weekly for 5 weeks and then assayed for antibodies to these proteins. Animals were then separated into the following groups: chow sham, chow MAA immunized, high fat sham, and high fat MAA immunized. The high fat animals were fed a Western diet with 2-thiouracil for 12 weeks, bled every 3 weeks, and serum assayed for the presence of circulating MAA antibodies. Results Prior to feeding with high fat diet, rats immunized with MAA-modified protein had a significant increase (P<0.001) in serum antibodies directed against these modified proteins compared to controls (N of 4 per group). Following feeding of high fat diet antibody concentrations increased 6 fold in the high fat MAA immunized group compared to the chow MAA immunized group (P<0.05). Antibodies in the high fat sham and chow sham had only minimal increases in antibodies to these proteins. Conclusions These data demonstrate that following immunization with MAA-modified proteins, circulating antibodies are produced that increase following consumption of a high fat Western diet. It suggests that MAA-modified proteins are produced at low levels following normal diet, producing antibodies which act as a normal clearance method for altered protein. When high fat consumption increases these antibody levels are increased in response to the oxidative stress. Implications Use of these antibodies as a biomarker in the future may help predict the onset or progression of atherosclerosis.

scholarly journals High fat diet alters gut microbiota but not spatial working memory in early middle-aged Sprague Dawley rats

PLoS ONE ◽  
2019 ◽  
Vol 14 (5) ◽  
pp. e0217553 ◽  
Author(s):  
Nikita Girish Deshpande ◽  
Juhi Saxena ◽  
Tristan G. Pesaresi ◽  
Casey Dylan Carrell ◽  
Grayson Breneman Ashby ◽  
...  
Keyword(s):  
Working Memory ◽  
Gut Microbiota ◽  
High Fat Diet ◽  
Spatial Working Memory ◽  
Sprague Dawley ◽  
High Fat ◽  
Middle Aged ◽  
Sprague Dawley Rats
Sign in / Sign up

Export Citation Format

Share Document