scholarly journals UPLC-ESI-MS/MS and HPTLC Method for Quantitative Estimation of Cytotoxic Glycosides and Aglycone in Bioactivity Guided Fractions of Solanum nigrum L.

2017 ◽  
Vol 8 ◽  
Author(s):  
Karishma Chester ◽  
Sarvesh Paliwal ◽  
Washim Khan ◽  
Sayeed Ahmad
Keyword(s):  
Quantitative Estimation ◽  
Solanum Nigrum ◽  
Esi Ms ◽  
Solanum Nigrum L ◽  
Hptlc Method

scholarly journals In Vitro Propagation, Development and Validation of HPTLC Method for Quantification of Steroidal Glycoalkaloid Solasodine in In Vitro Culture and Plant Parts of Solanum Nigrum

2018 ◽  
Vol 34 (6) ◽  
pp. 2728-2734 ◽  
Author(s):  
Mohd Amir ◽  
Niyaz Ahmad ◽  
Mohammad Sarafroz ◽  
Mahvish Jamal ◽  
Faheem Hyder Pottoo ◽  
...  
Keyword(s):  
High Performance ◽  
Control Method ◽  
Quantitative Estimation ◽  
Statistical Investigation ◽  
Solanum Nigrum ◽  
Nodal Segment ◽  
Standard Drug ◽  
Plant Parts ◽  
Hptlc Method

Solanum nigrum Linn. (Family: solanaceae), has medicinal values, commonly exploited for its active constituents which are of high pharmaceutical importance. The plant contains different type of natural compounds, and one of most important glycoalkaloid in S. nigrum is solasodine. Nodal segment of S. nigrum was cultured on MS media added with several concentrations of 2, 4-dichlorophenoxy acetic acid (2, 4- D) (0.5ppm –2ppm) for callus induction and development. For shoot induction, MS medium complemented with different dilutions of 6- benzyl adenine (6- BA) (0.5ppm–2ppm) was used. A novel, rapid, and simple high performance thin layer chromatographic method for quantitative estimation of triterpenoid glycoalkaloid. Solasodine was spotted on silica gel 60F254 TLC plate using chloroform: methanol (8.75: 1.25; v/v) as mobile phase. Anisaldehyde-sulfuric acid in methanol used as derivatizing reagent and quantified by densitometric study at 366 nm. This scheme was found to give a sharp and compact spot for the standard drug (Rf = 0.67 ± 0.04). The linearity of this method was found to be a range of 100-1000 ng per spot with LOD and LOQ of 23.78 ng per spot and 67.09 ng per spot; respectively. This method has shown recoveries of solasodine in the range from 98.23% -100.88 %, with low intra and inter-day % RSD. Statistical investigation of the data presented that the method is selective, accurate, precise and reproducible for the study of solasodine. The established and developed HPTLC method would be a significant parameter for the analysis of quality control method of herbal drugs and formulations.

Mechanical Factors of Infl uence on Biological Effi ciency of Solanum Nigrum L.

2014 ◽  
Vol 1 (2) ◽  
pp. 20-23
Author(s):  
O. Ivashchenko ◽  
O. Ivashchenko
Keyword(s):  
Solanum Nigrum ◽  
Black Nightshade ◽  
Effi Ciency ◽  
Weed Plants ◽  
Sensitivity Level ◽  
Solanum Nigrum L ◽  
Small Plot ◽  
Mechanical Factors ◽  
The Moment ◽  
Working Out

Aim. Studying of black nightshade young plants’ response to the induced mechanical stresses. Methods. Researches conducted in small plot fi eld experiments. Results. Change of sensitivity level of Solanum nigrum L. plants depending on phases of their development at the moment of damage of elevated parts has been proven. Owing to loss of the surface capable to photosynthesis, there is an essential decrease in volumes of photosynthesis at plants of weed survived and their possibilities of ontogeny passage. The deep induced dis- stresses reduce biological effi ciency of plants, their ability to accumulate weight and to form seeds and even lead them to death. Conclusions. The defi ned principles of response of weed plants to the induced mechanical dis-stresses are can be used for working out and ecological receptions of crops protection from weeds.

scholarly journals Phytochemical composition of leaves and stems of Solanum nigrum L. and Solanum dulcamara L. (Solanaceae) from Bulgaria

2021 ◽  
Vol 1031 (1) ◽  
pp. 012091
Author(s):  
V T Popova ◽  
M A Stoyanova ◽  
T A Ivanova ◽  
A S Stoyanova ◽  
I Z Dimitrova-Dyulgerova
Keyword(s):  
Solanum Nigrum ◽  
Solanum Dulcamara ◽  
Phytochemical Composition ◽  
Solanum Nigrum L

scholarly journals Anatomical study of Solanum nigrum L. from Solanaceae family growing in Iraq

2021 ◽  
Vol 1879 (2) ◽  
pp. 022003
Author(s):  
Muazaz Azeez Al-Hadeethi ◽  
Ali T Al-Taie ◽  
A. Farman Al-Rawi
Keyword(s):  
Anatomical Study ◽  
Solanum Nigrum ◽  
Solanum Nigrum L ◽  
Solanaceae Family

HPLC/Tandem Mass Spectrometric Studies on Steroidal Saponins: An Example of Quantitative Determination of Shatavarin IV from Dietary Supplements Containing Asparagus racemosus

2014 ◽  
Vol 97 (6) ◽  
pp. 1497-1502 ◽  
Author(s):  
Dada Patil ◽  
Manish Gautam ◽  
Sunil Gairola ◽  
Suresh Jadhav ◽  
Bhushan Patwardhan
Keyword(s):  
Acetic Acid ◽  
Dietary Supplements ◽  
Quantitative Estimation ◽  
Multiple Reaction Monitoring ◽  
Asparagus Racemosus ◽  
Steroidal Saponin ◽  
Esi Ms ◽  
Tandem Mass Spectrometric ◽  
Positive Ion

Abstract Asparagus racemosus (AR) is a popular botanical present in several Ayurvedic medicines and nutritional and dietary supplements with immunomodulatory, galactogogue, and anticancer activity. A steroidal saponin known as shatavarin IV is one of the active constituents of AR. A new, selective, and rapid HPLC/MS/MS method has been developed and validated for quantitative estimation of shatavarin IV in crude, processed, and marketed samples of AR. The analytes were separated on a Luna C18 column using simple isocratic elution with water (0.1% acetic acid)–acetonitrile (0.1% acetic acid; 70 + 30, v/v) at a flow rate of 0.8 mL/min. The analytes were detected by electrospray ionization (ESI)-MS/MS and quantified using multiple reaction monitoring techniques in the positive ion mode. The method showed excellent linearity (r2 > 0.998) over the concentration range of 7.5 to 254 ng/mL with LOD of 2.5 ng/mL. Precision (RSD) and accuracy (recovery) were found in the ranges of 2.00 to 5.15 and 102 to 110%, respectively. The validated HPLC/ESI-MS/MS method was successfully applied to the quantification of shatavarin IV in crude, processed, and marketed (single or multiherb) AR samples. Therefore, this method could be used for QC and standardization of pharmaceutical or nutritional products containing AR.

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