scholarly journals Corrigendum: Magnitude Assessment of Adult Neurogenesis in the Octopus vulgaris Brain Using a Flow Cytometry-Based Technique

2019 ◽  
Vol 10 ◽  
Author(s):  
Anna Di Cosmo ◽  
Carla Bertapelle ◽  
Antonio Porcellini ◽  
Gianluca Polese
Keyword(s):  
Flow Cytometry ◽  
Adult Neurogenesis ◽  
Octopus Vulgaris ◽  
Magnitude Assessment

scholarly journals Magnitude Assessment of Adult Neurogenesis in the Octopus vulgaris Brain Using a Flow Cytometry-Based Technique

2018 ◽  
Vol 9 ◽  
Author(s):  
Anna Di Cosmo ◽  
Carla Bertapelle ◽  
Antonio Porcellini ◽  
Gianluca Polese
Keyword(s):  
Flow Cytometry ◽  
Adult Neurogenesis ◽  
Octopus Vulgaris ◽  
Magnitude Assessment

scholarly journals Cognitive Stimulation Induces Differential Gene Expression in Octopus vulgaris: The Key Role of Protocadherins

Biology ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 196
Author(s):  
Valeria Maselli ◽  
Gianluca Polese ◽  
Al-Sayed Al-Soudy ◽  
Maria Buglione ◽  
Anna Di Cosmo
Keyword(s):  
Gene Expression ◽  
Differential Gene Expression ◽  
Adult Neurogenesis ◽  
Control Cell ◽  
Enriched Environment ◽  
Cognitive Stimulation ◽  
Octopus Vulgaris ◽  
Brain Areas ◽  
Differential Gene ◽  
The Brain

Octopuses are unique invertebrates, with sophisticated and flexible behaviors controlled by a high degree of brain plasticity, learning, and memory. Moreover, in Octopus vulgaris, it has been demonstrated that animals housed in an enriched environment show adult neurogenesis in specific brain areas. Firstly, we evaluated the optimal acclimatization period needed for an O. vulgaris before starting a cognitive stimulation experiment. Subsequently, we analyzed differential gene expression in specific brain areas in adult animals kept in tested (enriched environment), wild (naturally enriched environment), and control conditions (unenriched environment). We selected and sequenced three protocadherin genes (PCDHs) involved in the development and maintenance of the nervous system; three Pax genes that control cell specification and tissue differentiation; the Elav gene, an earliest marker for neural cells; and the Zic1 gene, involved in early neural formation in the brain. In this paper, we evaluated gene expression levels in O. vulgaris under different cognitive stimulations. Our data shows that Oct-PCDHs genes are upregulated in the learning and lower motor centers in the brain of both tested and wild animals (higher in the latter). Combining these results with our previous studies on O. vulgaris neurogenesis, we proposed that PCDH genes may be involved in adult neurogenesis processes, and related with their cognitive abilities.

A novel flow cytometry-based technique to measure adult neurogenesis in the brain

2011 ◽  
Vol 119 (1) ◽  
pp. 165-175 ◽  
Author(s):  
Robert Spoelgen ◽  
Annette Meyer ◽  
Anja Moraru ◽  
Friederike Kirsch ◽  
Angela Vogt-Eisele ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Adult Neurogenesis ◽  
The Brain

Automation of cytokine flow cytometry assays

2004 ◽  
Vol 9 (1) ◽  
pp. 5-9 ◽  
Author(s):  
J DUNNE ◽  
H MAECKER
Keyword(s):  
Flow Cytometry ◽  
Cytokine Flow Cytometry

Flow cytometry using the monoclonal antibody CD10-Pe/Cy5 is a useful tool to identify follicular lymphoma cells

2001 ◽  
Vol 66 (2) ◽  
pp. 100-106 ◽  
Author(s):  
M. Bellido ◽  
E. Rubiol ◽  
J. Ubeda ◽  
O. Lopez ◽  
C. Estivill ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Flow Cytometry ◽  
Follicular Lymphoma ◽  
Lymphoma Cells

Flow Cytometry

1990 ◽  
Keyword(s):  
Flow Cytometry

MxA as biomarker for the activity of interferon-alpha in patients with chronic hepatitis: Measurement by flow cytometry

2010 ◽  
Vol 48 (05) ◽  
Author(s):  
W Tillinger ◽  
R Jilch ◽  
P Wunsch ◽  
L Kramer ◽  
S Knapp
Keyword(s):  
Flow Cytometry ◽  
Chronic Hepatitis ◽  
Interferon Alpha

Effects of a Monoclonal Antibody to Glycoprotein IIb/IIIa (P256) and of Enzymically Derived Fragments of P256 on Human Platelets

1991 ◽  
Vol 65 (04) ◽  
pp. 432-437 ◽  
Author(s):  
A W J Stuttle ◽  
M J Powling ◽  
J M Ritter ◽  
R M Hardisty
Keyword(s):  
Monoclonal Antibody ◽  
Flow Cytometry ◽  
Platelet Aggregation ◽  
Calcium Ions ◽  
Human Platelet ◽  
Human Platelets ◽  
In Vivo Detection ◽  
Fibrinogen Binding ◽  
Specific Antagonist

SummaryThe anti-platelet monoclonal antibody P256 is currently undergoing development for in vivo detection of thrombus. We have examined the actions of P256 and two fragments on human platelet function. P256, and its divalent fragment, caused aggregation at concentrations of 10−9−3 × 10−8 M. A monovalent fragment of P256 did not cause aggregation at concentrations up to 10−7 M. P256–induced platelet aggregation was dependent upon extracellular calcium ions as assessed by quin2 fluorescence. Indomethacin partially inhibited platelet aggregation and completely inhibited intracellular calcium mobilisation. Apyrase caused partial inhibition of aggregation. Aggregation induced by the divalent fragment was dependent upon fibrinogen and was inhibited by prostacyclin. Aggregation induced by the whole antibody was only partially dependent upon fibrinogen, but was also inhibited by prostacyclin. P256 whole antibody was shown, by flow cytometry, to induce fibrinogen binding to indomethacin treated platelets. Monovalent P256 was shown to be a specific antagonist for aggregation induced by the divalent forms. In–111–labelled monovalent fragment bound to gel-filtered platelets in a saturable and displaceable manner. Monovalent P256 represents a safer form for in vivo applications

Sign in / Sign up

Export Citation Format

Share Document