scholarly journals Transcriptome Characterization and Expression Analysis of Chemosensory Genes in Chilo sacchariphagus (Lepidoptera Crambidae), a Key Pest of Sugarcane

2021 ◽  
Vol 12 ◽  
Author(s):  
Jianbai Liu ◽  
Huan Liu ◽  
Jiequn Yi ◽  
Yongkai Mao ◽  
Jihu Li ◽  
...  
Keyword(s):  
Host Location ◽  
Chemical Cues ◽  
Gene Families ◽  
Chemosensory Proteins ◽  
Chilo Sacchariphagus ◽  
Chemosensory Genes ◽  
Olfactory Reception ◽  
Major Pest ◽  
Insect Chemoreception ◽  
Pheromone Binding Proteins

Insect chemoreception involves many families of genes, including odourant/pheromone binding proteins (OBP/PBPs), chemosensory proteins (CSPs), odourant receptors (ORs), ionotropic receptors (IRs), and sensory neuron membrane proteins (SNMPs), which play irreplaceable roles in mediating insect behaviors such as host location, foraging, mating, oviposition, and avoidance of danger. However, little is known about the molecular mechanism of olfactory reception in Chilo sacchariphagus, which is a major pest of sugarcane. A set of 72 candidate chemosensory genes, including 31 OBPs/PBPs, 15 CSPs, 11 ORs, 13 IRs, and two SNMPs, were identified in four transcriptomes from different tissues and genders of C. sacchariphagus. Phylogenetic analysis was conducted on gene families and paralogs from other model insect species. Quantitative real-time PCR (qRT-PCR) showed that most of these chemosensory genes exhibited antennae-biased expression, but some had high expression in bodies. Most of the identified chemosensory genes were likely involved in chemoreception. This study provides a molecular foundation for the function of chemosensory proteins, and an opportunity for understanding how C. sacchariphagus behaviors are mediated via chemical cues. This research might facilitate the discovery of novel strategies for pest management in agricultural ecosystems.

Chemical Cues for Host Location by the Chestnut Gall Wasp, Dryocosmus kuriphilus

2010 ◽  
Vol 37 (1) ◽  
pp. 49-56 ◽  
Author(s):  
Giacinto S. Germinara ◽  
Antonio De Cristofaro ◽  
Giuseppe Rotundo
Keyword(s):  
Host Location ◽  
Chemical Cues ◽  
Gall Wasp ◽  
Dryocosmus Kuriphilus

Identification and analysis of chemosensory genes encoding odorant-binding proteins, chemosensory proteins and sensory neuron membrane proteins in the antennae of Lissorhoptrus oryzophilus

2021 ◽  
pp. 1-11
Author(s):  
Yu Pan ◽  
Xinxin Zhang ◽  
Zhun Wang ◽  
Lizhong Qi ◽  
Xinsheng Zhang ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Sensory Neuron ◽  
Binding Proteins ◽  
Mate Location ◽  
Lissorhoptrus Oryzophilus ◽  
Neuron Membrane ◽  
Odorant Binding Proteins ◽  
Chemosensory Proteins ◽  
Chemosensory Genes ◽  
Odorant Binding

Abstract The rice water weevil, Lissorhoptrus oryzophilus Kuschel (Coleoptera: Curculionidae), is a destructive pest that causes damage to rice crops worldwide. The olfactory system is critical for host or mate location by weevils, but only limited information about the molecular mechanism of olfaction-related behaviour has been reported in this insect. In this study, we conducted SMRT-seq transcriptome analysis and obtained 54,378 transcripts, 38,706 of which were annotated. Based on these annotations, we identified 40 candidate chemosensory genes, including 31 odorant-binding proteins (OBPs), six chemosensory proteins (CSPs) and three sensory neuron membrane proteins (SNMPs). Phylogenetic analysis showed that LoryOBPs, LoryCSPs and LorySNMPs were distributed in various clades. The results of tissue expression patterns indicated that LoryOBPs were highly abundant in the antennae, whereas LoryCSPs were highly abundant not only in the antennae but also in the abdomen, head and wings. Our findings substantially expand the gene database of L. oryzophilus and may serve as a basis for identifying novel targets to disrupt key olfactory genes, potentially providing an eco-friendly strategy to control this pest in the future.

scholarly journals Transcriptomic analysis and molecular docking reveal genes involved in the response of Aedes aegypti larvae to an essential oil extracted from Eucalyptus

2021 ◽  
Vol 15 (7) ◽  
pp. e0009587
Author(s):  
Ivana Sierra ◽  
Jose Manuel Latorre-Estivalis ◽  
Lucila Traverso ◽  
Paula V. Gonzalez ◽  
Ariel Aptekmann ◽  
...  
Keyword(s):  
Molecular Docking ◽  
Essential Oil ◽  
Aedes Aegypti ◽  
Vector Control ◽  
Gene Families ◽  
Control Group ◽  
Mosquito Vector ◽  
Rna Seq ◽  
Chemosensory Proteins ◽  
The Impact

Background Aedes aegypti (L.) is an urban mosquito, vector of several arboviruses that cause severe diseases in hundreds of million people each year. The resistance to synthetic insecticides developed by Ae. aegypti populations worldwide has contributed to failures in vector control campaigns, increasing the impact of arbovirus diseases. In this context, plant-derived essential oils with larvicidal activity could be an attractive alternative for vector control. However, the mode of action and the detoxificant response of mosquitoes to plant derived compounds have not been established, impairing the optimization of their use. Methods and findings Here we compare gene expression in Ae. aegypti larvae after 14 hrs of exposure to Eucalyptus camaldulensis essential oil with a control group exposed to vehicle (acetone) for the same lapse, by using RNA-Seq. We found differentially expressed genes encoding for cuticle proteins, fatty-acid synthesis, membrane transporters and detoxificant related gene families (i.e. heat shock proteins, cytochromes P450, glutathione transferases, UDP-glycosyltransferases and ABC transporters). Finally, our RNA-Seq and molecular docking results provide evidence pointing to a central involvement of chemosensory proteins in the detoxificant response in mosquitoes. Conclusions and significance Our work contributes to the understanding of the physiological response of Ae. aegypti larvae to an intoxication with a natural toxic distilled from Eucalyptus leafs. The results suggest an involvement of most of the gene families associated to detoxification of xenobiotics in insects. Noteworthy, this work provides important information regarding the implication of chemosensory proteins in the detoxification of a natural larvicide. Understanding the mode of detoxification of Eucalyptus distilled compounds could contribute to their implementation as a tool in mosquito control.

scholarly journals Odorant Binding Proteins and Chemosensory Proteins in Episyrphus balteatus (Diptera: Syrphidae): Molecular Cloning, Expression Profiling, and Gene Evolution

2020 ◽  
Vol 20 (4) ◽  
Author(s):  
Hui-Ru Jia ◽  
Lin-Lin Niu ◽  
Yu-Feng Sun ◽  
Yong-Qiang Liu ◽  
Kong-Ming Wu
Keyword(s):  
Binding Proteins ◽  
Gene Evolution ◽  
Purifying Selection ◽  
Episyrphus Balteatus ◽  
Odorant Binding Proteins ◽  
Chemosensory Proteins ◽  
Survival And Reproduction ◽  
Insect Chemoreception ◽  
Molecular Components ◽  
Odorant Binding

Abstract Aphidophagous syrphids (Diptera: Syrphidae) are important insects in agroecosystems for pollination and biological control. Insect chemoreception is essential for these processes and for insect survival and reproduction; however, molecular determinants is not well understood for these beneficial insects. Here, we used recent transcriptome data for the common hoverfly, Episyrphus balteatus, to characterize key molecular components of chemoreception: odorant-binding proteins (OBPs) and chemosensory proteins (CSPs). Six EbalCSPs and 44 EbalOBPs were cloned from this species, and sequence analysis showed that most share the characteristic hallmarks of their protein family, including a signal peptide and conserved cysteine signature. Some regular patterns and key conserved motifs of OBPs and CSPs in Diptera were identified using the online tool MEME. Motifs were also compared among the three OBP subgroups. Quantitative real-time PCR (qRT-PCR) showed that most of these chemosensory genes were expressed in chemosensory organs, suggesting these genes have chemoreceptive functions. An overall comparison of the Ka/Ks values of orthologous genes in E. balteatus and another predatory hoverfly species to analyze the evolution of these olfactory genes showed that OBPs and CSPs are under strong purifying selection. Overall, our results provide a molecular basis for further exploring the chemosensory mechanisms of E. balteatus, and consequently, may help us to understand the tritrophic interactions among plants, herbivorous insects, and natural enemies.

scholarly journals Chemosensory Gene Families in the Oligophagous Pear Pest Cacopsylla chinensis (Hemiptera: Psyllidae)

Insects ◽  
2019 ◽  
Vol 10 (6) ◽  
pp. 175 ◽  
Author(s):  
Ji-Wei Xu ◽  
Xiu-Yun Zhu ◽  
Qiu-Jie Chao ◽  
Yong-Jie Zhang ◽  
Yu-Xia Yang ◽  
...  
Keyword(s):  
Insect Behavior ◽  
Gene Families ◽  
Tissue Expression ◽  
Specific Expression ◽  
Female Head ◽  
Chemosensory Gene ◽  
Chemosensory Genes ◽  
Male Head ◽  
Chemosensory Systems ◽  
Odorant Binding

Chemosensory systems play an important role in insect behavior, and some key associated genes have potential as novel targets for pest control. Cacopsylla chinensis is an oligophagous pest and has become one of the main pests of pear trees, but little is known about the molecular-level means by which it locates its hosts. In this study, we assembled the head transcriptome of C. chinensis using Illumina sequencing, and 63,052 Unigenes were identified. A total of 36 candidate chemosensory genes were identified, including five different families: 12 odorant binding proteins (OBPs), 11 chemosensory proteins (CSPs), 7 odorant receptors (ORs), 4 ionotropic receptors (IRs), and 2 gustatory receptors (GRs). The number of chemosensory gene families is consistent with that found in other Hemipteran species, indicating that our approach successfully obtained the chemosensory genes of C. chinensis. The tissue expression of all genes using quantitative real-time PCR (qRT-PCR) found that some genes displayed male head, female head, or nymph-biased specific/expression. Our results enrich the gene inventory of C. chinensis and provide valuable resources for the analysis of the functions of some key genes. This will help in developing molecular targets for disrupting feeding behavior in C. chinensis.

scholarly journals Chemical Ecology of Egg Parasitoids Associated with True Bugs

2012 ◽  
Vol 2012 ◽  
pp. 1-11 ◽  
Author(s):  
Eric Conti ◽  
Stefano Colazza
Keyword(s):  
Pest Control ◽  
Host Location ◽  
Chemical Cues ◽  
Egg Parasitoids ◽  
Insect Herbivores ◽  
Host Searching ◽  
Biological Pest Control ◽  
Insect Eggs ◽  
True Bugs ◽  
Selection Strategies

Parasitoids representing some 15 families of Hymenoptera develop in insect eggs; three of these families, Platygastridae (= Scelionidae), Mymaridae, and Encyrtidae, are associated with Heteroptera. Several species of heteropteran egg parasitoids are or may be important for biological pest control. Successful parasitism of insect herbivores by insect parasitoids arises through several phases of host searching, which lead female wasps to the vicinity of, or in contact with, their hosts. During the host location process, females encounter and explore a variety of stimuli, among which chemical cues (i.e., semiochemicals or infochemicals) play a pivotal role. Female parasitoids are under selection pressure to efficiently invest their limited time on the location and exploitation of host-derived stimuli. In general, the levels of reliability and detectability of a particular stimulus are inversely correlated. Female parasitic wasps adopt differing strategies to solve this dilemma. In this paper we focus on the various host selection strategies employed by heteropteran egg parasitoids and possible means whereby the chemically mediated behavior of these wasps may be exploited to enhance biological pest control.

Sign in / Sign up

Export Citation Format

Share Document