Selenium hyperaccumulators harbor a diverse endophytic bacterial community characterized by high selenium resistance and plant growth promoting properties

Abstract Purpose Arctium lappa L. is one of the medicinal and food homologous plants in China, which is rich in nutrients and medicinal ingredients. The use of plant growth promoting (PGP) endophytic bacteria is a useful alternative in agricultural production to reduce the use of chemical fertilizers. The aim of this study was to analysis the diversity of endophytic bacteria in different cultivars of A. lappa L. collected from two different geographical locations in China and evaluate PGP traits of the isolates and their potential PGP ability in greenhouse condition. Methods Endophytic bacterial community was investigated by culture-dependent and culture-independent methods. Isolates were screened and investigated for multiple PGP traits, and representative strains were inoculated host seedlings to evaluate the growth promoting effect. Results A total of 348 endophytic bacteria were obtained and they distributed into four phyla, 30 genera and 73 different species. In addition, high throughput sequencing revealed more abundant bacterial community, including 17 bacterial phyla, and 207 different known genera. A high proportion of PGP traits were detected, including production of indole acetic acid, siderophore, ammonia and phosphate solubilization. Four representative strains with multiple PGP traits of the most prevalent genera were further selected for host inoculation and growth promoting evaluation, and they significantly increase seedlings length, root length and fresh weight. Conclusion This study demonstrated that A. lappa L. harbors abundant endophytic bacteria, and plant genotype and geographical origin affect their composition. Moreover, some endophytic bacteria showed good potential for the development of microbial fertilizer in the future.

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Bacterial community structure and detection of putative plant growth-promoting rhizobacteria associated with plants grown in Chilean agro-ecosystems and undisturbed ecosystems

Biology and Fertility of Soils ◽

10.1007/s00374-014-0935-6 ◽

2014 ◽

Vol 50 (7) ◽

pp. 1141-1153 ◽

Cited By ~ 24

Author(s):

Milko A. Jorquera ◽

Nitza G. Inostroza ◽

Lorena M. Lagos ◽

Patricio J. Barra ◽

Luis G. Marileo ◽

...

Keyword(s):

Community Structure ◽

Bacterial Community ◽

Plant Growth ◽

Bacterial Community Structure ◽

Plant Growth Promoting Rhizobacteria ◽

Plant Growth Promoting ◽

Growth Promoting

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Comparison of the bacterial community and characterization of plant growth-promoting rhizobacteria from different genotypes of Chrysopogon zizanioides (L.) Roberty (Vetiver) rhizospheres

The Journal of Microbiology ◽

10.1007/s12275-009-0048-3 ◽

2009 ◽

Vol 47 (4) ◽

pp. 363-370 ◽

Cited By ~ 16

Author(s):

Juliana Mendes Monteiro ◽

Renata Estebanez Vollú ◽

Marcia Reed Rodrigues Coelho ◽

Celuta Sales Alviano ◽

Arie Fitzgerald Blank ◽

...

Keyword(s):

Bacterial Community ◽

Plant Growth ◽

Plant Growth Promoting Rhizobacteria ◽

Chrysopogon Zizanioides ◽

Plant Growth Promoting ◽

Growth Promoting

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Response of Soil Bacterial Community and Pepper Plant Growth to Application of Bacillus thuringiensis KNU-07

Agronomy ◽

10.3390/agronomy10040551 ◽

2020 ◽

Vol 10 (4) ◽

pp. 551 ◽

Cited By ~ 2

Author(s):

HyungWoo Jo ◽

Setu Bazie Tagele ◽

Huy Quang Pham ◽

Min-Chul Kim ◽

Seung-Dae Choi ◽

...

Keyword(s):

Bacterial Community ◽

Plant Growth ◽

Soil Bacterial Community ◽

Specific Primer ◽

Soil Bacterial Community Structure ◽

Plant Growth Promoting ◽

Growth Promoting ◽

Soil Bacterial ◽

Pgpr Strain ◽

Pepper Plants

Many Bacillus species are among the plant growth-promoting rhizobacteria (PGPR) that promote the growth of many different plant species. This study aimed to investigate the effects of Bacillus thuringiensis KNU-07 on the growth of pepper plants and the soil microbiota. We also designed primers specific for the strain KNU-07 to monitor the population in pepper-cultivated soil. Accordingly, a strain-specific primer pair was designed using a database constructed from 16,160 complete bacterial genomes. We employed quantitative PCR (qPCR) to track the abundance of the strain KNU-07 introduced into pepper-cultivated soil using the strain-specific primers. Our study revealed that the strain was found to possess plant growth-promoting (PGP) activities, and it promoted the growth of pepper plants. The soil bacterial community structure due to the application of the PGPR strain was significantly changed after six weeks post-inoculation. In addition, based on qPCR analysis, the population of the introduced strain declined over time. In this study, application of a PGPR strain increased the growth of pepper plants and changed the soil bacterial community structure. The successful results of monitoring of a bacterial strain’s population using a single strain-specific primer pair can provide important information about the quantification of bio-inoculants under non-sterile soil conditions.

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Isolation and Evaluation of Bacteria Exhibiting Multiple Plant Growth Traits in the Rhizosphere of Yellow Bell Pepper (Capsicum chinense)

Journal of Advances in Microbiology ◽

10.9734/jamb/2019/v14i430070 ◽

2019 ◽

pp. 1-6

Author(s):

E. C. Chinakwe ◽

N. U. Nwogwugwu ◽

V. I. Ibekwe ◽

P. O. Chinakwe ◽

E. O. Egbadon ◽

...

Keyword(s):

Bacterial Community ◽

Plant Growth ◽

Rhizosphere Soil ◽

Growth Traits ◽

Soil Samples ◽

Bell Pepper ◽

Capsicum Chinense ◽

Plant Growth Promoting ◽

Growth Promoting ◽

Plant Growth Promoting Traits

Aim: The study identified and evaluated bacteria exhibiting multiple plant growth traits in the Rhizosphere of Yellow Bell Pepper (Capsicum chinense). Study Design: Seeds of Capsicum chinense were planted in a soil and allowed to grow. After five eeks of planting, soil samples from the rhizosphere were collected and the bacterial community present in the rhizosphere soil of Capsicum chinense was studied. The isolated organisms were assessed for their ability to produce plant growth promoting traits. Place and Duration of Study: This study was carried out at an agricultural research farmland in the Federal University of Technology, Owerri, Nigeria. Methodology: Seeds of Capiscum chinense were planted in the soil samples in a greenhouse. Rhizosphere soil was collected for analysis to identify the bacterial composition of the rhizosphere soil. Results: In this study the presence of Bacillus cereus, Staphylococcus aureus, Corynebacterium sp, Enterococcus feacalis and Bacillus polymyxa were evident in the rhizosphere samples collected. All isolates showed multiple plant growth promoting traits except Staphylococcus aureushich was positive for hydrogen cyanide production only. Conclusion: The results from this study showed that the bacterial community present in the soil can be used to effect significant vegetative crop yield and agricultural production. The isolated rhizobacteria can be formulated as bio-fertilizers or bioinnoculants, etc.

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Plant Growth Promoting Rhizobacteria Alleviate Aluminum Toxicity and Ginger Bacterial Wilt in Acidic Continuous Cropping Soil

Frontiers in Microbiology ◽

10.3389/fmicb.2020.569512 ◽

2020 ◽

Vol 11 ◽

Author(s):

Shuting Zhang ◽

Qipeng Jiang ◽

Xiaojiao Liu ◽

Liehua Liu ◽

Wei Ding

Keyword(s):

Bacterial Community ◽

Plant Growth ◽

Bacterial Wilt ◽

Aluminum Toxicity ◽

Bacterial Community Composition ◽

Plant Growth Promoting Rhizobacteria ◽

Continuous Cropping ◽

Aluminum Ions ◽

Plant Growth Promoting ◽

Growth Promoting

Long-term monoculture cropping is usually accompanied by soil acidification and microbial community shifts. Soil aluminum ions are dissolved under acidic condition (pH < 5.0), and the resulting aluminum bioavailability can cause toxic effects in plants. In this study, we investigated the bacterial community compositions and aluminum toxicity in fields monocultured with ginger for 35 years, 15 years, and 1 year. Within these fields are ginger plants without and with ginger bacterial wilt disease. The results confirmed that the degree of aluminum toxicity in the diseased soil was more severe than that in the healthy soil. Continuous cropping can significantly increase the bacterial diversity and change the bacterial community composition of ginger rhizosphere soil. The relative abundance of plant growth-promoting rhizobacteria (PGPRs) was increased in the soils used for the continuous cropping of ginger. Additionally, aluminum toxicity had a significant positive correlation with Bacillus, Pseudomonas, Arthrobacter, and Serratia in healthy soils. Based on these results, aluminum stress may stimulate the increase of PGPRs (Bacillus, Pseudomonas, Arthrobacter, and Serratia), thereby alleviating ginger aluminum toxicity and bacterial wilt in extremely acidic soil (pH < 4.5).

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