scholarly journals Variations in Early Response of Grapevine Wood Depending on Wound and Inoculation Combinations with Phaeoacremonium aleophilum and Phaeomoniella chlamydospora

2016 ◽  
Vol 7 ◽  
Author(s):  
Romain J. G. Pierron ◽  
Jérôme Pouzoulet ◽  
Christel Couderc ◽  
Elodie Judic ◽  
Stéphane Compant ◽  
...  
Keyword(s):  
Early Response ◽  
Phaeomoniella Chlamydospora ◽  
Phaeoacremonium Aleophilum

A method to detect and quantify Phaeomoniella chlamydospora and Phaeoacremonium aleophilum DNA in grapevine-wood samples

2013 ◽  
Vol 97 (23) ◽  
pp. 10163-10175 ◽  
Author(s):  
Jérôme Pouzoulet ◽  
Nathalie Mailhac ◽  
Christel Couderc ◽  
Xavier Besson ◽  
Jean Daydé ◽  
...  
Keyword(s):  
Phaeomoniella Chlamydospora ◽  
Phaeoacremonium Aleophilum

Genetic diversity in populations of the fungi Phaeomoniella chlamydospora and Phaeoacremonium aleophilum on grapevine in France

2002 ◽  
Vol 51 (1) ◽  
pp. 85-96 ◽  
Author(s):  
B. Borie ◽  
L. Jacquiot ◽  
I. Jamaux-Despréaux ◽  
P. Larignon ◽  
J.-P. Péros
Keyword(s):  
Genetic Diversity ◽  
Phaeomoniella Chlamydospora ◽  
Phaeoacremonium Aleophilum

scholarly journals Susceptibility of Grapevine Pruning Wounds and Symptom Development in Response to Infection by Phaeoacremonium aleophilum and Phaeomoniella chlamydospora

Plant Disease ◽  
2007 ◽  
Vol 91 (9) ◽  
pp. 1100-1104 ◽  
Author(s):  
A. Eskalen ◽  
A. J. Feliciano ◽  
W. D. Gubler
Keyword(s):  
Disease Incidence ◽  
Wound Age ◽  
Cabernet Sauvignon ◽  
Symptom Development ◽  
Thompson Seedless ◽  
Phaeomoniella Chlamydospora ◽  
Phaeoacremonium Aleophilum ◽  
Pruning Wound ◽  
Successful Infection ◽  
Vascular Discoloration

Fresh to 10-month-old pruning wounds on grapevine cvs. Thompson Seedless and Cabernet Sauvignon were inoculated with Phaeoacremonium aleophilum or Phaeomoniella chlamydospora at approximately 1 × 106 spores/ml. Successful infection was determined by isolation of the pathogen from necrotic margins of cankers or from vascular discoloration assessed 4 months after each inoculation date. Disease incidence decreased as the length of time between pruning and inoculation increased; however, wounds remained susceptible for up to 4 months. Vascular discoloration was significantly less extensive in the noninoculated control than in inoculated spurs. Reduction of shoot length in both cultivars varied depending on pruning wound age at the time of inoculation. In a separate study, inoculation of fresh pruning wounds of Cabernet Sauvignon resulted in successful infection regardless of the time of pruning from February to December.

scholarly journals Differing Alterations of Two Esca Associated Fungi, Phaeoacremonium aleophilum and Phaeomoniella chlamydospora on Transcriptomic Level, to Co-Cultured Vitis vinifera L. calli

PLoS ONE ◽  
2016 ◽  
Vol 11 (9) ◽  
pp. e0163344 ◽  
Author(s):  
Jochen Fischer ◽  
Stéphane Compant ◽  
Romain J. G. Pierron ◽  
Markus Gorfer ◽  
Alban Jacques ◽  
...  
Keyword(s):  
Vitis Vinifera ◽  
Vitis Vinifera L ◽  
Phaeomoniella Chlamydospora ◽  
Phaeoacremonium Aleophilum ◽  
Transcriptomic Level

Ascospore Release of Togninia minima, Cause of Esca and Grapevine Decline in California

2005 ◽  
Vol 6 (1) ◽  
pp. 16 ◽  
Author(s):  
Suzanne Rooney-Latham ◽  
A. Eskalen ◽  
W. D. Gubler
Keyword(s):  
Fungal Pathogens ◽  
Video Camera ◽  
Disease Cycle ◽  
Phaeomoniella Chlamydospora ◽  
Grapevine Decline ◽  
Phaeoacremonium Aleophilum ◽  
Naturally Occurring ◽  
Ascospore Release ◽  
Video Documentation ◽  
Different Strains

Esca and grapevine decline are important diseases affecting both young and mature grapevines worldwide. In California, these diseases are caused primarily by the fungal pathogens Phaeomoniella chlamydospora and Phaeoacremonium aleophilum. Perithecia of Togninia minima, the newly-described teleomorph of Phaeoacremonium aleophilum, were produced by mating different strains in culture. Using a video camera, the process of emerging asci and release of ascospores from perithecia was filmed and presented herein for review. Furthermore, naturally occurring perithecia were discovered in infected California vineyards. These studies provide video documentation of the method of ascospore release of Togninia minima and suggest the importance of ascospore release in the disease cycle of esca in California vineyards. Accepted for publication 7 December 2004. Published 9 February 2005.

scholarly journals Real-Time PCR Detection of Phaeomoniella chlamydospora and Phaeoacremonium aleophilum

2012 ◽  
Vol 78 (11) ◽  
pp. 3985-3991 ◽  
Author(s):  
Maria Teresa Martín ◽  
Rebeca Cobos ◽  
Laura Martín ◽  
Lorena López-Enríquez
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Control Measures ◽  
Economic Losses ◽  
Dna Purification ◽  
Time Saving ◽  
Content Type ◽  
Phaeomoniella Chlamydospora ◽  
Phaeoacremonium Aleophilum ◽  
Detection And Identification

ABSTRACTPhaeomoniella chlamydosporaandPhaeoacremonium aleophilumare the two main fungal causal agents of Petri disease and esca. Both diseases cause significant economic losses to viticulturalists. Since no curative control measures are known, proactive defensive measures must be taken. An important aspect of current research is the development of sensitive and time-saving protocols for the detection and identification of these pathogens. Real-time PCR based on the amplification of specific sequences is now being used for the identification and quantification of many infective agents. The present work reports real-time PCR protocols for identification ofP. chlamydosporaandP. aleophilum. Specificity was demonstrated against purified DNA from 60P. chlamydosporaisolates or 61P. aleophilumisolates, and no amplification was obtained with 54 nontarget DNAs. The limits of detection (i.e., DNA detectable in 95% of reactions) were around 100 fg forP. chlamydosporaand 50 fg forP. aleophilum. Detection was specific and sensitive forP. chlamydosporaandP. aleophilum. Spores ofP. chlamydosporaandP. aleophilumwere detected without the need for DNA purification. The established protocols detected these fungi in wood samples after DNA purification.P. chlamydosporawas detectable without DNA purification and isolation in 67% of reactions. The detection of these pathogens in wood samples has great potential for use in pathogen-free certification schemes.

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