scholarly journals Serosurvey of Anti-Toxoplasma gondii Antibodies in Homeless Persons of São Paulo City, Southeastern Brazil

2020 ◽  
Vol 8 ◽  
Author(s):  
Laís Giuliani Felipetto ◽  
Pedro Irineu Teider-Junior ◽  
Felipe Fortino Verdan da Silva ◽  
Ana Carolina Yamakawa ◽  
Louise Bach Kmetiuk ◽  
...  
Author(s):  
Laís Giuliani Felipetto ◽  
Pedro Irineu Teider-Junior ◽  
Felipe Fortino Verdan da Silva ◽  
Ana Carolina Yamakawa ◽  
Louise Bach Kmetiuk ◽  
...  

Abstract Background: Seroprevalence of Toxoplasma gondii has been extensively studied in a variety of vulnerable populations. However, no study has focused on homeless populations. Accordingly, the present study aimed to assess the seroprevalence of anti-T. gondii antibodies and the risk factors associated in homeless persons from homeless shelter of São Paulo city, southeastern Brazil. In addition, anti-HIV antibodies and associated risk of T. gondii and HIV coinfection have been evaluated.Methods: Anti-T. gondii antibodies were detected by indirect fluorescent antibody test. In addition, anti-HIV levels were testedby chemiluminescence enzyme immunoassay, with positive samples confirmed by rapid immunoblot assay.Results: Overall, IgG anti-T. gondii seropositivity was found in 43/120 (35.8%) homeless persons, with endpoint titers varying from 16 to 1,024. The only two pregnant womentested were negative for IgM by chemiluminescence enzyme immunoassay, with normal parturition and clinically healthy newborns in both cases. There were no statistical differences in the risk factors for anti-T. gondii serology (p>0.05). Anti-HIV seropositivity was found in 2/120 (1.7%) homeless persons, confirmed as HIV-1. One HIV seropositive individual was also sero-reactive to IgG anti-T.gondii, and both were negative to IgM anti-T. gondii.Conclusions: This is the first study that reports the serosurvey of T. gondii in homeless persons worldwide. Despite the limited sample size available in the present study, our findings have shown that the prevalence of anti-T. gondii antibodies in homeless persons herein was lower than the general population, probably due to homeless diet habit of eating mainly processed food intake. No statistical differences were found regarding risk factors for anti-T. gondii exposure in homeless persons.


2010 ◽  
Vol 19 (4) ◽  
pp. 237-243 ◽  
Author(s):  
Fernanda Sartori Lima de Godoi ◽  
Sandra Mayumi Nishi ◽  
Hilda Fátima de Jesus Pena ◽  
Solange Maria Gennari

This study aimed to diagnose experimental and natural Toxoplasma gondii infection in pigeons (Columba livia) by serological, biological and molecular techniques. Twelve pigeons, free of infection, were inoculated with 50 sporulated oocysts of T. gondii (VEG sample) and four remained uninfected controls. Four birds (three infected and one control) were euthanized at 15, 30, 45 and 60 days post-infection (dpi), and their tissues were used to perform a bioassay in mice and nested-PCR using B1 gene as target. Blood was obtained weekly and it was tested for the presence of anti-T. gondii antibodies by the indirect fluorescent antibody test (IFAT) and modified agglutination test (MAT). Seven (58.3%) out of 12 inoculated pigeons were positive by serological techniques and titers ranged between 1:40 and 1:5120 by MAT and between 1:512 and 1:4096 by IFAT. Complete agreement was seen between the results obtained by serological techniques and nested-PCR in seven positive birds. In the bioassay in mice, five (41.7%) out of 12 pigeons inoculated were positive to T. gondii. Only one pigeon died at 23 dpi due to toxoplasmosis. A second study with free-living pigeons was performed for detection of anti-T. gondii antibodies. Birds were captured in the municipalities of São Paulo, Ibiúna and Sorocaba, São Paulo State, Southeastern Brazil. All 126 free-living birds were negative to anti-T. gondii antibodies by MAT (titer < 1:5). Bioassays were performed in mice with tissues from all captured birds and T. gondii was not isolated in any pigeon.


Author(s):  
Laís Giuliani Felipetto ◽  
Pedro Irineu Teider-Junior ◽  
Felipe Fortino Verdan da Silva ◽  
Anahi Chechia do Couto ◽  
Louise Bach Kmetiuk ◽  
...  

2015 ◽  
Vol 24 (3) ◽  
pp. 379-382 ◽  
Author(s):  
Solange Maria Gennari ◽  
Maria Halina Ogrzewalska ◽  
Herbert Sousa Soares ◽  
Danilo Gonçalves Saraiva ◽  
Adriano Pinter ◽  
...  

Toxoplasma gondii is a protozoan parasite that infects a large spectrum of warm-blooded animals, including humans. Small rodents and marsupials play an important role in the epidemiology of T. gondii because they are sources of infection for domestic and feral cats. Serum samples from 151 rodents and 48 marsupials, captured in the Atlantic Forest, São Paulo State, southeastern Brazil, were analyzed for the presence of T. gondii antibodies. Antibodies detected by the modified agglutination test (MAT ≥ 25) were found in 8.6% (13/151) of the rodents and 10.4% (5/48) of the marsupials, with titers ranging from 25 to 6400 and from 25 to 3200, respectively for the rodents and marsupials. Three of the eight species of rodents (Akodon spp., Oligoryzomys nigripesand Rattus norvegicus), and one from the four marsupial species (Didelphis aurita) presented positive animals. T. gondii was described for the first time in the rodent Oligoryzomys nigripes.


Check List ◽  
2010 ◽  
Vol 6 (2) ◽  
pp. 217 ◽  
Author(s):  
Mariana Juventina Magrini ◽  
Paula Beatriz Araujo ◽  
Marcio Uehara-Prado

Terrestrial Isopods were sampled in four protected Atlantic Forest areas located in Serra do Mar, state of São Paulo, southeastern Brazil. A total of 2,217 individuals of six species (Atlantoscia sp., Benthana werneri, Pseudodiploexochus tabularis, Pudeoniscus obscurus, Styloniscus spinosus and Trichorhina sp.) were captured in pitfall traps. The exotic species S. spinosus is recorded for the first time for the Americas. Another introduced species, P. tabularis, previously recorded only from the state of Rio Grande do Sul, had its geographic distribution extended to the state of São Paulo. The most abundant isopods in this study belong to an undescribed species of Atlantoscia.


2014 ◽  
Vol 206 (3-4) ◽  
pp. 293-296 ◽  
Author(s):  
Aline D. Cabral ◽  
Sandra R.N. D’Auria ◽  
Maria C.G.O. Camargo ◽  
Adriana R. Rosa ◽  
Miriam M. Sodré ◽  
...  

2003 ◽  
Vol 89 (4) ◽  
pp. 870-871 ◽  
Author(s):  
L. E O. Yai ◽  
W. A. Cañon-Franco ◽  
V. C. Geraldi ◽  
M. E L. Summa ◽  
M. C G. O. Camargo ◽  
...  

2010 ◽  
Vol 88 (1) ◽  
pp. 104-106 ◽  
Author(s):  
Welber Daniel Zanetti Lopes ◽  
Thaís Rabelo dos Santos ◽  
Ricardo dos Santos da Silva ◽  
Walter Matheus Rossanese ◽  
Fernando Augusto de Souza ◽  
...  

Zootaxa ◽  
2013 ◽  
Vol 3599 (2) ◽  
pp. 178-188 ◽  
Author(s):  
ITAMAR A. MARTINS ◽  
HUSSAM ZAHER

A new species of the genus Holoaden is described from the Atlantic forest of the state of São Paulo, Brazil, being restricted to primary or slightly disturbed high altitude cloud forests along the northeastern portion of the Serra do Mar. The typelocality is determined as Estação Ecológica de Bananal, in the Municipality of Bananal, State of São Paulo, Brazil. The new species is characterized by its moderate body size (female 42.6–44.2 mm SVL; male 37.2–38.5 mm SVL) with long and slender limbs, a head wider than long, a highly glandular dorsum, covered by well developed macroglands that extend to the internasal region, thigh and tibia, and an intense dark brown dorsal coloration and dark grey ventral surface.


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