scholarly journals Characterization of Nuclear Progesterone Receptor Isoforms in the Term Equine Placenta

2021 ◽  
Vol 8 ◽  
Author(s):  
Ahmed M. Nagy ◽  
Swanand R. Sathe ◽  
Attia H. Atta ◽  
Abdel Mohsen M. Hammam ◽  
Walter H. Hsu
Keyword(s):  
Progesterone Receptor ◽  
Placental Tissue ◽  
Progesterone Receptor Isoforms ◽  
Protein Levels ◽  
Receptor Isoforms ◽  
Protein Expressions ◽  
Integral Role ◽  
Nuclear Progesterone Receptor

In equine parturition, the role of progestins along with the nuclear progesterone receptor (nPR) signaling pathway in the placenta is not completely clarified. The progestins play an integral role in maintaining myometrial quiescence during the late stage of pregnancy via acting on nPR isoforms (PRA and PRB; PRB is more active than PRA). The current study aimed to determine the PRA and PRB expressions in the term equine placenta at the gene and protein levels. Six term equine placentas were used in this study. Reverse transcription polymerase chain reaction (RT-PCR) was used to quantify the mRNA expression for PRA and PRB. The protein expression was detected using the Western Blot technique. The results revealed that the mRNA and protein expressions for PRA were significantly higher (P < 0.0001) in the term equine placental tissue compared to the mRNA and protein expressions of PRB. These results demonstrated that nPRs are detectable in the term placenta of mares and PRA is the dominant isoform expressed. The present findings raised the possibility that the PRA plays an important role in the parturition process and expulsion of the placenta in mares.

scholarly journals Role of nuclear progesterone receptor isoforms in uterine pathophysiology

2014 ◽  
Vol 21 (2) ◽  
pp. 155-173 ◽  
Author(s):  
B. Patel ◽  
S. Elguero ◽  
S. Thakore ◽  
W. Dahoud ◽  
M. Bedaiwy ◽  
...  
Keyword(s):  
Progesterone Receptor ◽  
Progesterone Receptor Isoforms ◽  
Receptor Isoforms ◽  
Nuclear Progesterone Receptor

Nuclear progesterone receptor isoforms and their functions in the female reproductive tract

2011 ◽  
Vol 14 (1) ◽  
pp. 149-158 ◽  
Author(s):  
R. Rękawiecki ◽  
M. Kowalik ◽  
J. Kotwica
Keyword(s):  
Progesterone Receptor ◽  
Receptor Antagonist ◽  
Reproductive System ◽  
Reproductive Tract ◽  
Physiological Effect ◽  
Female Reproductive Tract ◽  
Target Cells ◽  
Progesterone Receptor Isoforms ◽  
Receptor Isoforms ◽  
Nuclear Progesterone Receptor

Nuclear progesterone receptor isoforms and their functions in the female reproductive tract Progesterone (P4), which is produced by the corpus luteum (CL), creates proper conditions for the embryo implantation, its development, and ensures proper conditions for the duration of pregnancy. Besides the non-genomic activity of P4 on target cells, its main physiological effect is caused through genomic action by the progesterone nuclear receptor (PGR). This nuclear progesterone receptor occurs in two specific isoforms, PGRA and PGRB. PGRA isoform acts as an inhibitor of transcriptional action of PGRB. The inactive receptor is connected with chaperone proteins and attachment of P4 causes disconnection of chaperones and unveiling of DNA binding domain (DBD). After receptor dimerization in the cells' nucleus and interaction with hormone response element (HRE), the receptor coactivators are connected and transcription is initiated. The ratio of these isoforms changes during the estrous cycle and reflects the different levels of P4 effect on the reproductive system. Both isoforms, PGRA and PGRB, also show a different response to the P4 receptor antagonist activity. Connection of the antagonist to PGRA can block PGRB, but acting through the PGRB isoform, P4 receptor antagonist may undergo conversion to a strongly receptor agonist. A third isoform, PGRC, has also been revealed. This isoform is the shortest and does not have transcriptional activity. Alternative splicing and insertion of additional exons may lead to the formation of different PGR isoforms. This paper summarizes the available data on the progesterone receptor isoforms and its regulatory action within the female reproductive system.

The role of progesterone receptor isoforms in the pregnant myometrium

2014 ◽  
Vol 102 (3) ◽  
pp. e128
Author(s):  
M. Peavey ◽  
X. Li ◽  
M. Wetendorf ◽  
C. Yallampalli ◽  
J. Lydon ◽  
...  
Keyword(s):  
Progesterone Receptor ◽  
Progesterone Receptor Isoforms ◽  
Receptor Isoforms

The role of DNA methylation and histone acetylation in the regulation of progesterone receptor isoforms expression in human astrocytoma cell lines

Steroids ◽  
2013 ◽  
Vol 78 (5) ◽  
pp. 500-507 ◽  
Author(s):  
Valeria Hansberg-Pastor ◽  
Aliesha González-Arenas ◽  
Miguel A. Peña-Ortiz ◽  
Elizabeth García-Gómez ◽  
Mauricio Rodríguez-Dorantes ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Progesterone Receptor ◽  
Histone Acetylation ◽  
Cell Lines ◽  
Progesterone Receptor Isoforms ◽  
Receptor Isoforms ◽  
Astrocytoma Cell ◽  
Human Astrocytoma ◽  
Human Astrocytoma Cell

Role of Progesterone Receptor Isoforms in Human Astrocytomas Growth

2011 ◽  
pp. 57-63
Author(s):  
Ignacio Camacho-Arroyo ◽  
Valeria Hansberg-Pastor ◽  
Edith Cabrera-Muñoz ◽  
Olivia Tania Hernández-Hernández ◽  
Aliesha González-Arenas
Keyword(s):  
Progesterone Receptor ◽  
Progesterone Receptor Isoforms ◽  
Receptor Isoforms ◽  
Human Astrocytomas

Role of Progesterone Receptor Isoforms in Female Sexual Behavior Induced by Progestins in Rats

2009 ◽  
Vol 90 (1) ◽  
pp. 73-81 ◽  
Author(s):  
Christian Guerra-Araiza ◽  
Porfirio Gómora-Arrati ◽  
Marcos García-Juárez ◽  
Alejandra Armengual-Villegas ◽  
Alfredo Miranda-Martínez ◽  
...  
Keyword(s):  
Sexual Behavior ◽  
Progesterone Receptor ◽  
Female Sexual Behavior ◽  
Progesterone Receptor Isoforms ◽  
Receptor Isoforms

Luteotropic and luteolytic factors regulate mRNA and protein expression of progesterone receptor isoforms A and B in the bovine endometrium

2016 ◽  
Vol 28 (7) ◽  
pp. 907 ◽  
Author(s):  
Robert Rekawiecki ◽  
Magdalena Karolina Kowalik ◽  
Jan Kotwica
Keyword(s):  
Progesterone Receptor ◽  
Protein Expression ◽  
Mrna Expression ◽  
Expression Analysis ◽  
Oestrous Cycle ◽  
Nitric Oxide Donor ◽  
Mrna Sequence ◽  
Progesterone Receptor Isoforms ◽  
Protein Levels ◽  
Receptor Isoforms

The aim of the present study was to examine the effects of luteotropic and luteolytic factors on the mRNA and protein levels of progesterone receptor isoforms A (PGRA) and B (PGRB) in the bovine endometrium. Endometrial slices from Days 6–10 and 17–20 of the oestrous cycle were treated with LH (100 ng mL–1), oestradiol (E2; 1 × 10–8 M), prostaglandin (PG) E2 (1 × 10–6 M) and PGF2α (1 × 10–6 M) and the nitric oxide donor NONOate (1 × 10–4 M); these treatments lasted for 6 h for mRNA expression analysis and 24 h for protein expression analysis. On Days 6–10 of the oestrous cycle PGRAB (PGRAB; the entire PGRA mRNA sequence is common to the PGRB mRNA sequence) mRNA expression in endometrial slices was enhanced by E2 treatment (P < 0.001), whereas PGRB mRNA expression was increased by LH (P < 0.001), E2 (P < 0.05) and NONOate (P < 0.05) treatment. On Days 17–20, PGRAB mRNA expression increased after E2 (P < 0.001) and PGE2 (P < 0.05) treatment; PGRB mRNA expression was increased by PGE2 (P < 0.05) and PGF2α (P < 0.01) treatment, but decreased by LH (P < 0.05). On Days 6–10 protein levels of PGRA were stimulated by E2 (P < 0.01), whereas PGRB protein levels were increased by LH (P < 0.05) and E2 (P < 0.05). On Days 17–20 of the oestrous cycle, PGRA protein levels were enhanced by E2 (P < 0.05) and PGF2α (P < 0.05), whereas PGRB protein levels were stimulated by PGE2 (P < 0.05) and PGF2α (P < 0.001). These data suggest that luteotropic and luteolytic factors affect PGRA and PGRB mRNA and protein levels, and this may regulate the effects of progesterone on endometrial cells.

scholarly journals The role of epithelial Progesterone Receptor Isoforms in embryo implantation

iScience ◽  
2021 ◽  
pp. 103487
Author(s):  
Rong Li ◽  
Xiaoqiu Wang ◽  
Zhenyao Huang ◽  
Jayani Balaji ◽  
Tae Hoon Kim ◽  
...  
Keyword(s):  
Progesterone Receptor ◽  
Embryo Implantation ◽  
Progesterone Receptor Isoforms ◽  
Receptor Isoforms
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