Effect of One-Day Delaying CIDR Administration in 5-Day Cosynch Protocol in Dairy Heifers

Effect of one-day delaying progesterone administration at the beginning of 5-day Cosynch protocol was investigated in Holstein heifers for the first artificial insemination (AI). Heifers received a synchronized ovulation and timed AI (TAI) with CIDR inserted on day (d) 0 (CIDR-5; n = 206) or d 1 (CIDR-4; n = 192). In both group, GnRH was administered on d 0 followed by a single PGF2α injection and CIDR removal five days later from GnRH. On d 8, TAI and GnRH administration were concurrently conducted. Heifers detected in estrus up to 24 h prior to TAI were inseminated without GnRH administration. Rates of ovulation, accessory CL formation and new dominant follicle development following initial GnRH injection did not differ between groups. P/AI did not differ between CIDR-4 (44.3%, [85/192]) and CIDR-5 (51.9%, [107/206]) groups, respectively. Pregnancy per AI (P/AI) was significantly (p < 0.01) declined as heifers’ age (12–13, 14, 15, 16 and17–21 months) proceeded in CIDR-4 group (55.6%, 52.1%, 37.9%, 35.7%, 32.4%) compared to those in CIDR-5 group (60.0%, 50.0%, 53.9%, 51.5%, 46.2%) respectively. In conclusion, there is no benefit for delaying CIDR administration in 5-day Cosynch protocol in dairy heifers. However, higher P/AI in CIDR-5 group in older heifers can be considered for reproductive management.

Download Full-text

Hormonal manipulations in the 5-day timed artificial insemination protocol to optimize estrous cycle synchrony and fertility in dairy heifers

Journal of Dairy Science ◽

10.3168/jds.2013-7093 ◽

2013 ◽

Vol 96 (11) ◽

pp. 7054-7065 ◽

Cited By ~ 34

Author(s):

F.S. Lima ◽

E.S. Ribeiro ◽

R.S. Bisinotto ◽

L.F. Greco ◽

N. Martinez ◽

...

Keyword(s):

Estrous Cycle ◽

Artificial Insemination ◽

Dairy Heifers ◽

Timed Artificial Insemination

Download Full-text

The Effect of A PRID®Delta-Based Estrus Synchronization Protocol On Pregnancy Rates In Cows With No Estrus Symptoms

10.21203/rs.3.rs-1199382/v1 ◽

2022 ◽

Author(s):

Murat Can Demir ◽

Cihan Kaçar ◽

Umut Çağın Arı ◽

Semra Kaya ◽

Oğuz Merhan ◽

...

Keyword(s):

Pregnancy Rate ◽

Artificial Insemination ◽

Prostaglandin F2α ◽

Pregnancy Rates ◽

Dominant Follicle ◽

Serum Progesterone ◽

Blood Samples ◽

Follicle Diameter ◽

Follicle Size ◽

Equine Chorionic Gonadotropin

Abstract The present study aimed to determine the effects of progesterone-based gonadotropin-releasing hormone (GnRH), prostaglandin F2α (PGF2α), and equine chorionic gonadotropin (eCG) injections on progesterone profiles and pregnancy rates in cows with no estrus symptoms within 60 days after parturition. A total of 80 cows were included in the study. All animals had the progesterone-releasing device PRID®Delta placed intravaginally for nine days with an injection of GnRH. On the eighth day, PGF2α was injected, and PRID®Delta was removed from the vagina on day nine. Artificial insemination was carried out 60 hours after PRID®Delta removal. In half of the animals (n = 40), 600 IU of eCG was injected when PRID®Delta was removed on the ninth day before artificial insemination 60 hours later. Blood samples were taken from the tail vein on days 0 and 8 to determine progesterone levels. The pregnancy rate in the group that received eCG was 37.5%, while it was 27.5% in those that did not (P = 0.4). While the dominant follicle diameter was 15.5 mm in cows injected with eCG during timed artificial insemination, the follicle diameter was 12.4 mm in cows with no eCG injection (P <0.001). There were no differences in serum progesterone values in blood samples taken until the time of artificial insemination. However, progesterone values in the blood taken during artificial insemination were 0.94 ng/ml in the eCG- group and 0.72 ng/ml in the eCG+ group (P <0.05). As a result, it was determined that eCG injections, in addition to progesterone-based GnRH and PGF2α applications, increased the pregnancy rates in cows without symptoms of estrus. The dominant follicle diameter was larger in cows treated with eCG during artificial insemination; however, follicle size did not increase the pregnancy rate.

Download Full-text

Economic Benefits of Reproductive Management, Synchronization of Estrus, and Artificial Insemination in Beef Cattle and Sheep

New Technologies in Animal Breeding ◽

10.1016/b978-0-12-123450-8.50018-6 ◽

1981 ◽

pp. 243-254 ◽

Cited By ~ 1

Author(s):

E. KEITH INSKEEP ◽

JOHN B. PETERS

Keyword(s):

Beef Cattle ◽

Artificial Insemination ◽

Economic Benefits ◽

Reproductive Management ◽

Cattle And Sheep

Download Full-text

Defining the gonadotrophin requirement for the selection of a single dominant follicle in cattle

Reproduction Fertility and Development ◽

10.1071/rd19060 ◽

2020 ◽

Vol 32 (3) ◽

pp. 322 ◽

Cited By ~ 1

Author(s):

Jin G. Gong ◽

Bruce K. Campbell ◽

Robert Webb

Keyword(s):

Major Effect ◽

Follicle Development ◽

Preovulatory Follicle ◽

Ultrasound Scanning ◽

Dominant Follicle ◽

Initial Exposure ◽

Preovulatory Follicles ◽

Gonadotrophin Releasing Hormone ◽

Follicular Response ◽

Selection Of

The aim was to define the pattern and physiological concentrations of FSH and LH required for the selection of a single dominant follicle in mono-ovulatory species. A series of five experiments was carried out using gonadotrophin-releasing hormone agonist-induced hypogonadal heifers. Animals were infused with different patterns of either FSH and/or LH followed by an ovulatory dose of human chorionic gonadotrophin. Follicular response was monitored by ultrasound scanning and blood samples were collected to measure concentrations of FSH, LH, oestradiol and progesterone. The main findings were: (1) physiological concentrations of FSH given as a continuous infusion and for an adequate duration, in the presence of basal LH, with or without LH pulses, are capable of inducing a superovulatory response, (2) initial exposure to FSH followed by LH pulses alone stimulate the development of multiple preovulatory follicles, confirming that ovarian follicles are capable of transferring dependence on gonadotrophins from FSH to LH, (3) while LH pulses appear not to have a major effect on the pattern of preovulatory follicle development, adequate LH pulsatile support is required for full oestradiol synthesis and (4) the duration of initial exposure to FSH and the ability to transfer the dependence from FSH to LH are critical for the selection of a single dominant follicle. In conclusion, this experimental series confirms that the duration of initial exposure to FSH and the ability of the selected follicle to transfer its gonadotrophic dependence from FSH to LH are critical for the selection of a single dominant follicle in cattle.

Download Full-text

175 FOLLICULAR DEVELOPMENT AND OVARIAN BLOOD FLOW IN MARES WITH EARLY OR LATE OVULATION POSTPARTUM

Reproduction Fertility and Development ◽

10.1071/rdv27n1ab175 ◽

2015 ◽

Vol 27 (1) ◽

pp. 178

Author(s):

K. M. Lemes ◽

L. A. Silva ◽

E. C. C. Celeghini ◽

M. A. Alonso ◽

G. Pugliesi ◽

...

Keyword(s):

Blood Flow ◽

Postpartum Period ◽

Follicular Development ◽

Ovarian Activity ◽

Follicle Development ◽

Follicular Growth ◽

Dominant Follicle ◽

Vascular Perfusion ◽

Early Postpartum ◽

Linear Probe

The postpartum period is characterised by the rapid uterine involution process and return of ovarian activity (foal heat), resulting in a fertile oestrus in most of the mares. However, the follicular development and selection processes during this period are not completely known in horses. We aimed to study the characteristics of follicular growth and vascular perfusion in the ovary during the early postpartum period in mares that demonstrated oestrous behaviour and had early (<10 days) or late (≥10 days) ovulation. Ten mares were scanned daily from the first day postpartum (Day 1) until the day of the first postpartum ovulation (Day 0). The animals were split in the early (n = 3) and late (n = 7) ovulation groups (averaged interval between parturition and ovulation: 8.0 ± 0.0 and 14.7 ± 1.2 days, respectively). For ultrasound exams a Duplex B-mode and colour Doppler instrument (M5VET®, Mindray, Shenzhen, China) was used with a multifrequency linear probe. Data were analysed for the main effects of group, day, and their interaction using the PROC MIXED procedure of SAS software (version 9.3, SAS Institute Inc., Cary, NC, USA). For the follicular growth, no difference (P > 0.05) was detected between the groups when the data were analysed for the days relative to ovulation (from Day 7 to Day 1). However, the dominant follicle was larger (P < 0.05) in the early-ovulated group (37.2 ± 1.6 v. 21.9 ± 1.1) in all days during early postpartum (Day 1 to Day 7). The number of follicles with >25 mm diameter was also greater (P < 0.05) in the early-ovulated group (1.1 ± 0.1 v. 0.1 ± 0.1) during the first 3 days postpartum. In addition, the late-ovulated mares showed greater number of follicles with 20–25 mm during Day 4 to Day 7 (2.0 ± 0.2 v. 0.7 ± 0.1). For the blood flow characteristics, no difference (P > 0.05) was detected in the coloured signals of blood flows in the follicular wall of the dominant follicle or in the ovarian pedicle ipsilateral to the largest follicle. Therefore, the characteristics of the follicle growth on the preceding days of ovulation were similar between the early- and late-ovulated mares and consistent with the follicular dynamics expected in non-pregnant and non-lactating mares. However, when the data were analysed for the days relative to parturition, a greater follicle development was present in mares that ovulate earlier during the postpartum period (<10 days). In conclusion, the results suggest that important events may occur previous to the parturition, resulting in an early follicle development, mainly in those mares that show heat signs and ovulate within 10 days postpartum. Research was supported by FAPESP process number 2010/10692-9 and CNPq process number 135954/2011-8.

Download Full-text