Differential Expression of ACTL8 Gene and Association Study of Its Variations with Growth Traits in Chinese Cattle

Mutations are heritable changes at the base level of genomic DNA. Furthermore, mutations lead to genetic polymorphisms and may alter animal growth phenotypes. Our previous study found that mutations in the bovine Actin-like protein 8 (ACTL8) gene may be involved in muscle growth and development. This study explored several mutations of the ACTL8 gene and their influence on body size in Chinese beef cattle, as well as tested the tissue expression profile of the ACTL8 gene in Qinchuan cattle at different ages. Five single nucleotide polymorphisms (SNPs) (including one synonymous mutation (c.2135552895G > A)) and two insertion/deletion polymorphisms (indels) were identified in the ACTL8 gene from 1138 cattle by DNA-seq, RFLP and other methods. Then, the expression profile of the ACTL8 gene in Qinchuan cattle showed that it was expressed in heart, spleen, lung, liver, muscle, and fat tissues. Moreover, the expression level of ACTL8 was increased with cattle growth (p < 0.01). The ACTL8 mRNA expression level in kidney and muscle tissues was the highest in the calves, while lowest in the fetal stage. Overall, we showed that the mutations could act as markers in beef molecular breeding and selection of the growth traits of cattle.

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Function of the Porcine TRPC1 Gene in Myogenesis and Muscle Growth

Cells ◽

10.3390/cells10010147 ◽

2021 ◽

Vol 10 (1) ◽

pp. 147

Author(s):

Yu Fu ◽

Peng Shang ◽

Bo Zhang ◽

Xiaolong Tian ◽

Ruixue Nie ◽

...

Keyword(s):

Transient Receptor Potential ◽

Growth Traits ◽

Expression Profiles ◽

Muscle Growth ◽

Receptor Potential ◽

Transient Receptor Potential Channel ◽

Nucleotide Polymorphisms ◽

Pig Breeds ◽

Muscle Tissues ◽

Transient Receptor

In animals, muscle growth is a quantitative trait controlled by multiple genes. Previously, we showed that the transient receptor potential channel 1 (TRPC1) gene was differentially expressed in muscle tissues between pig breeds with divergent growth traits base on RNA-seq. Here, we characterized TRPC1 expression profiles in different tissues and pig breeds and showed that TRPC1 was highly expressed in the muscle. We found two single nucleotide polymorphisms (SNPs) (C-1763T and C-1604T) in TRPC1 that could affect the promoter region activity and regulate pig growth rate. Functionally, we used RNAi and overexpression to illustrate that TRPC1 promotes myoblast proliferation, migration, differentiation, fusion, and muscle hypertrophy while inhibiting muscle degradation. These processes may be mediated by the activation of Wnt signaling pathways. Altogether, our results revealed that TRPC1 might promote muscle growth and development and plays a key role in Wnt-mediated myogenesis.

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Genetic variant of <i>SPARC</i> gene and its association with growth traits in Chinese cattle

Archives Animal Breeding ◽

10.5194/aab-63-31-2020 ◽

2020 ◽

Vol 63 (1) ◽

pp. 31-37 ◽

Cited By ~ 1

Author(s):

Danyang Zhang ◽

Jiawei Xu ◽

Peng Yang ◽

Yifan Wen ◽

Hua He ◽

...

Keyword(s):

Genetic Variant ◽

Growth Traits ◽

Expression Level ◽

Qinchuan Cattle ◽

Adult Cattle ◽

Chinese Cattle ◽

Muscle Tissues ◽

Selection For ◽

Dna Pool ◽

Sparc Expression

Abstract. SPARC is a cysteine-rich acidic secreted protein. It is a non-collagen component of bone, which is widely distributed in humans and animals and plays an important role. SPARC has been found in a variety of human cancers (breast cancer, stomach cancer, ovarian cancer, etc.) and diabetes-related research. Especially the muscle and fat metabolism are closely related. In this study, we used a DNA pool to detect a new SNP site (g.12454T > C). A total of 616 samples of four breeds of Qinchuan cattle (QC, n=176), Xianan cattle (XN, n=160), Pinan cattle (PN, n=136) and Jiaxian cattle (JX, n=144) were analyzed and identified with ARMS-PCR. In addition, we correlated SNP with growth traits and showed significant correlation with growth traits such as rump length, hip width, and body length (p<0.05). Moreover, we tested the SPARC gene expression level in different tissues belonging to XN adult cattle (n=3) and found its high expression in muscle tissues (relative to the kidney). Further, we found the SNP is able to increase the SPARC expression level in skeletal muscle (n=12). According to statistical data, this SNP site may be applied to a molecular marker of an early marker-assisted selection for early growth of beef cattle.

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