scholarly journals Modulated 3D Cross-Correlation Dynamic Light Scattering Applications for Optical Biosensing and Time-Dependent Monitoring of Nanoparticle-Biofluid Interactions

2020 ◽  
Vol 10 (24) ◽  
pp. 8969
Author(s):  
Silvia Schintke ◽  
Eleonora Frau
Keyword(s):  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Protein Interactions ◽  
Recent Progress ◽  
Cross Correlation ◽  
Translational Motion ◽  
Mouse Serum ◽  
Optical Biosensing ◽  
Model Protein ◽  
Protein Bovine Serum Albumin

This paper reviews dynamic light scattering (DLS) of gold nanoparticle-protein interactions for the model protein bovine serum albumin (BSA), as well as in complex biofluids, at the example of mouse serum. DLS data of nanorods of various aspect ratio, of proteins and of mouse serum are discussed in terms of the analysis of their hydrodynamic radii, leading to the distinction of rotational and translational motion as well as to the detection of agglomerates. We address in particular advances obtained by modulated 3D cross correlation dynamic light scattering and recent progress using the CORENN algorithm for analysis of DLS data.

scholarly journals Multi-Step Concanavalin A Phase Separation and Early-Stage Nucleation Monitored Via Dynamic and Depolarized Light Scattering

Crystals ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 620 ◽  
Author(s):  
Hévila Brognaro ◽  
Sven Falke ◽  
Celestin Nzanzu Mudogo ◽  
Christian Betzel
Keyword(s):  
Phase Separation ◽  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Protein Interactions ◽  
Concanavalin A ◽  
Early Stage ◽  
Protein Crystallization ◽  
Cluster Formation ◽  
Macromolecular Network

Protein phase separation and protein liquid cluster formation have been observed and analysed in protein crystallization experiments and, in recent years, have been reported more frequently, especially in studies related to membraneless organelles and protein cluster formation in cells. A detailed understanding about the phase separation process preceding liquid dense cluster formation will elucidate what has, so far, been poorly understood—despite intracellular crowding and phase separation being very common processes—and will also provide more insights into the early events of in vitro protein crystallization. In this context, the phase separation and crystallization kinetics of concanavalin A were analysed in detail, which applies simultaneous dynamic light scattering and depolarized dynamic light scattering to obtain insights into metastable intermediate states between the soluble phase and the crystalline form. A multi-step mechanism was identified for ConA phase separation, according to the resultant ACF decay, acquired after an increase in the concentration of the crowding agent until a metastable ConA gel intermediate between the soluble and final crystalline phases was observed. The obtained results also revealed that ConA is trapped in a macromolecular network due to short-range intermolecular protein interactions and is unable to transform back into a non-ergodic solution.

Relating Collective Diffusion, Protein–Protein Interactions, and Viscosity of Highly Concentrated Monoclonal Antibodies through Dynamic Light Scattering

2019 ◽  
Vol 58 (50) ◽  
pp. 22456-22471 ◽  
Author(s):  
Barton J. Dear ◽  
Amjad Chowdhury ◽  
Jessica J. Hung ◽  
Carl A. Karouta ◽  
Kishan Ramachandran ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Protein Interactions ◽  
Protein Protein Interactions

scholarly journals Particle sizing in strongly turbid suspensions with the one-beam cross-correlation dynamic light-scattering technique

1999 ◽  
Vol 38 (15) ◽  
pp. 3409 ◽  
Author(s):  
Anthony J. Adorjan ◽  
James A. Lock ◽  
Thomas W. Taylor ◽  
Padetha Tin ◽  
William V. Meyer ◽  
...  
Keyword(s):  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Cross Correlation ◽  
Particle Sizing ◽  
Scattering Technique ◽  
Dynamic Light Scattering Technique ◽  
The One ◽  
Light Scattering Technique

scholarly journals Dynamic light scattering for gold nanorod size characterization and study of nanorod–protein interactions

Gold Bulletin ◽  
2012 ◽  
Vol 45 (4) ◽  
pp. 187-195 ◽  
Author(s):  
Helin Liu ◽  
Nickisha Pierre-Pierre ◽  
Qun Huo
Keyword(s):  
Light Scattering ◽  
Dynamic Light Scattering ◽  
Protein Interactions ◽  
Gold Nanorod ◽  
Size Characterization
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