DISCREPANCY INR VALUE (INTERNATIONAL NORMALIZED RATIO) IN OPTICAL AND ELECTROMECHANICAL METHOD

Background: INR examination services at laboratories in hospitals and clinics use different methods and tools therefore, results of the INR examination are different. Purpose: To determine whether there are differences in the INR value between using the photo optic method and electromechanical method. Method: This study used a citrate blood sample with a ratio of blood and anticoagulant 9: 1 and used the Sysmex CA-600 device for the photo optic method and used the Thrombostat device for the electromechanical method. The sample consisted of 32 samples, namely 10 treatment samples to be analyzed and 22 normal samples. Using SPSS 25.0 to test data that was tested for normality test and in a different test. Result: The result of INR with the electromechanical method was significantly longer than INR with the photo optic method. In the hemolytic sample, the result was significantly higher than the INR result with normal samples. Conclusion: There are significant differences between results of the INR value using photo optic and electromechanical due to the difference in the detection principle between the two methods.

Study Of The Effect Of Polysaccharides On Hemostasis

The effect of sulfated polysaccharides on the hemostatic system in conditions in vitro. Platelet-rich plasma was obtained by centrifugation at 200 g for 10 minutes. The remaining citrate blood was further centrifuged at 1500 g for 10 min to obtain platelet-poor plasma. The antithrombin activity of the compounds was evaluated in vitro by their effect on the recalcification time, thrombin, and prothrombin time of human blood plasma stabilized with a 3.8% sodium citrate solution in the ratio of 9:1. In studies conducted on the blood plasma of rats, it was found that the studied compounds, to varying degrees, lengthen the APTT, APTT, prothrombin time. At the same time, anticoagulant activity was established to block one of the factors II, V, X. Polysaccharide exhibit a combined anticoagulant effect in the body, due to which they are classified as anticoagulant and antithrombin agents.

The challenges associated with short volume sodium citrate blood samples

In the laboratory, we often receive phone calls from our clinical colleagues with questions about decisions we have made that might affect their patient’s care. For example, enquiries about why we have rejected a sample or why we were unable to provide a particular result due to a pre-analytical or technical error. In this series of short articles, we want to try and address some of the most common questions we get asked, with the aim of educating the wider workforce in a way that is simple to understand, about the decisions we make in the laboratory. We hope that with a greater understanding, the number of avoidable errors might be reduced.In this article, we explore the pre-analytical challenges associated with short volume sodium citrate anti-coagulated blood samples and why these must be rejected.

Thrombin generation in different commercial sodium citrate blood tubes

Summary Background This study aimed to verify whether blood drawn into six different commercial coagulation tubes generated comparable results of thrombin generation. Methods Blood was sequentially collected from 20 healthy subjects into different brand and draw volume 3.2% sodium citrate tubes (4.3 mL Sarstedt, 3.0 mL Greiner, 2.7 mL Becton Dickinson, 2.0 mL Kima, 1.8 mL Sarstedt and 1.0 mL Greiner). Thrombin generation was measured in plasma with the fully-automated ST Genesia analyzer using the weakest trigger (STG-BleedScreen). Results Different values of lag time (LT), time to reach thrombin peak (TP), thrombin peak height (PH) and endogenous thrombin potential (ETP) were commonly found in different tubes. Thrombin generation was the lowest in 4.3 mL Sarstedt tubes and the highest in 1.0 mL Greiner tubes. Other tubes displayed intermediate values. In multiple comparisons, LT was significantly different in 6/15 cases (40%), whilst PH, TP and ETP were significantly different in 14/15 (93%), 13/15 (87%) and 13/15 (87%) cases. The mean percent bias of LT, PH, TP and ETP ranged between -6% and +1%, -27% and +116%, -22% and +8%, and between -18% and +65%. The intra-assay imprecision of LT, PH, TP and ETP was exceeded in 0/15 (0%), 13/15 (87%), 6/15 (40%) and 13/15 (87%) comparisons. The correlation of LT, PH, TP and ETP values in different tubes ranged between 0.718–0.971, 0.570–0.966, 0.725–0.977 and 0.101–0.904. Conclusions Blood collection for thrombin generation assays requires local standardization using identical tubes for brand and draw volume, and reference ranges calculated according to type of tubes.

PERBANDINGAN NILAI LAJU ENDAP DARAH (LED) ANTARA METODE WESTERGREN DENGAN METODE MIKRO ESR PADA PENDERITA TUBERKULOSIS PARU

One of the diseases that evoke an inflammatory reaction and affect the value of ESR is Pulmonary Tuberculosis. ESR examination suggested by ICSH is Westergren method. But there are many instances where the patient's venous blood sampling is so difficult that the blood obtained is small. One of the modification methods that can be used for ESR examination is the Micro ESR method. The purpose of this research is to know the comparison of ESR values ​​using Westergren and Micro ESR methods. The material used for this research is 3.8% citrate blood derived from 30 samples of patients with Pulmonary Tuberculosis at Krakatau Medika Hospital Cilegon. The average result of ESR Pulmonary Tuberculosis with Westergren method was 41,83 mm/hr and the mean value of ESR Pulmonary Tuberculosis with Micro ESR method was 33,03 mm/hr. The result of this research is processed by statistical test using Paired Sample T Test and got significance value (sig. 2-tailed) = 0,016. This value is smaller than the value of α = 0.05 which means there is a difference between the two examination variables, so the Micro ESR method could not be used for patients with Pulmonary Tuberculosis.

Screening test for detection of deep vein thromdosis and pulmonary embolism

Введение. Учитывая распространенность тромбозов, а каждый год в большинстве стран тромбоз глубоких вен (ТВГ) или тромбоэмболия легочной артерии (ТЭЛА) развивается у 80–120 человек на 100 тысяч населения, актуальным является использование скрининговых параметров гемостаза и оценка предрасположенности к тромбозам. Важным лабораторным маркером тромбоза и тромбоэмболии является Д-димер. Представляет интерес сравнение тестов различной чувствительности, имеющих признанное клиническое применение, для принятия решения о возможности их использования в ходе амбулаторного скрининга пациентов. Материалы и методы. Исследованы цитратные пробы крови 101 пациента (средний возраст — 41 ± 9,1 лет), среди которых были сформированы 3 группы: 69 человек, проходивших диспансерное обследование; 8 пациентов отделения интенсивной терапии; 24 пациента с подтвержденным сердечно-сосудистым заболеванием. Исследование содержания Д-димера в пробах цитратной плазмы выполняли одномоментно 2 методами: иммунотурбодиметрическим (ИТД) и иммунохемилюминесцентным (ИХЛ). Результаты. Получены результаты сравнения 2 методов исследования Д-димера. Коэффициент корреляции в разных группах пациентов составил 0,74–0,99 в зависимости от патологии, что свидетельствует о высокой степени соответствия результатов измерения Д-димера и возможности применения как метода ИТД, так и метода ИХЛ в скрининге пациентов с целью исключения ТВГ и ТЭЛА. Заключение. Проведенные исследования позволяют рассматривать метод ИХЛ для исследования содержания Д-димера на анализаторе Immulite 2000 XPi как скрининговый для амбулаторного догоспитального обследования пациентов. Introduction. Every year in most countries deep vein thrombosis (DVT) or pulmonary embolism (PE) develops in 80–120 people per 100 thousand population so screening of hemostatic parameters and assessment of predisposition to thrombosis is very actual problem. D-dimer is the important laboratory marker of thrombosis and thromboembolism. It is of interest to compare common clinical tests with diff erent sensitivities to determine whether they can be used during outpatient screening. Materials and methods. We examined citrate blood samples of 101 patients (mean age — 41 ± 9.1 years) who were divided into 3 groups: 69 persons passed the outpatient examination; 8 patients were in intensive care unit; 24 patients had confirmed cardiovascular disease. Measurement of D-dimer content in citrate plasma samples was carried out simultaneously by 2 methods: immunoturbodimetry (ITD) and immunochemiluminescence (IСL). Results. We obtained the results of D-dimer levels that were measured by 2 methods and calculated their relationships. The correlation coeffi cient in diff erent patient groups was 0.74–0.99 depending on their pathology. This fact shows a high degree of compliance of D-dimer results and the possibility of using both methods (ITD and IСL) in screening patients for excluding DVT and PE. Conclusion. Our researches allow to consider the IСL method for studying D-dimer content by Immulite 2000 XPi analyzer as a screening method for outpatient pre-hospital examination.