scholarly journals Characterization of Macrophage and Cytokine Interactions with Biomaterials Used in Negative-Pressure Wound Therapy

2021 ◽  
Vol 9 (1) ◽  
pp. 2
Author(s):  
Praveen Krishna Veerasubramanian ◽  
Victor C. Joe ◽  
Wendy F. Liu ◽  
Timothy L. Downing
Keyword(s):  
Negative Pressure ◽  
Negative Pressure Wound Therapy ◽  
Biological Effects ◽  
Wound Therapy ◽  
Tnf Α ◽  
Macrophage Adhesion ◽  
Cytokine Interactions ◽  
Control Surfaces

Macrophages are innate immune cells that help wounds heal. Here, we study the potential immunomodulatory effects of negative-pressure wound therapy (NPWT) materials on the macrophage inflammatory response. We compared the effects of two materials, Granufoam™ (GF) and Veraflo Cleanse™ (VC), on macrophage function in vitro. We find that both materials cause reduced expression of inflammatory genes, such as TNF and IL1B, in human macrophages stimulated with bacterial lipopolysaccharide (LPS) and interferon-gamma (IFNγ). Relative to adherent glass control surfaces, VC discourages macrophage adhesion and spreading, and may potentially sequester LPS/IFNγ and cytokines that the cells produce. GF, on the other hand, was less suppressive of inflammation, supported macrophage adhesion and spreading better than VC, and sequestered lesser quantities of LPS/IFNγ in comparison to VC. The control dressing material cotton gauze (CT) was also immunosuppressive, capable of TNF-α retention and LPS/IFNγ sequestration. Our findings suggest that NPWT material interactions with cells, as well as soluble factors including cytokines and LPS, can modulate the immune response, independent of vacuum application. We have also established methodological strategies for studying NPWT materials and reveal the potential utility of cell-based in vitro studies for elucidating biological effects of NPWT materials.

052 
Effect of Negative Pressure Wound Therapy on the Expression of TNF-α, IL-1β, MMP-2, MMP-3, and TIMP-1 in Wound Fluid of Adults with Pressure Ulcers

2008 ◽  
Vol 13 (2) ◽  
pp. A4-A27 ◽  
Author(s):  
J.K. Stechmiller ◽  
B. Childress ◽  
G. Stevens ◽  
D.V Kilpadi ◽  
G.S Schultz
Keyword(s):  
Negative Pressure ◽  
Negative Pressure Wound Therapy ◽  
Pressure Ulcers ◽  
Wound Therapy ◽  
Wound Fluid ◽  
Tnf Α

scholarly journals Associating Insulin Instillation with Negative Pressure Wound Therapy Reduces Insulin Resistance in Diabetic Patients with Infected Foot Wounds

2020 ◽  
Vol 7 (3) ◽  
pp. 1-8
Author(s):  
Xin-long Chen
Keyword(s):  
Insulin Resistance ◽  
Wound Healing ◽  
Negative Pressure ◽  
Negative Pressure Wound Therapy ◽  
Serum Levels ◽  
Treated Group ◽  
Post Treatment ◽  
Diabetic Patients ◽  
Wound Therapy ◽  
Tnf Α

In this work we investigated whether a joint treatment with insulin instillation and negative pressure wound therapy (NPWT) would reduce insulin resistance (IR) in diabetic patients with infected diabetic foot ulcers (DFUs) and improve their healing. We also studied the effects of insulin+NPWT treatment on the inflammatory response coupled with wound healing. Seventy-five diabetic patients with infected DFUs were recruited and randomly divided into equal (n=25) groups treated respectively with NPWT alone, NPWT+insulin, and conventional dressings (controls). Thereafter, the ulcers’ healing progress was assessed. The serum levels of Tumor Necrosis Factor-α (TNF-α), Interleukin-2 (IL-2), and Interleukin-6 (IL-6) were measured at 1 day before and at 7, 14, and 21 days after each treatment using specific double-antibody sandwich enzyme-linked immunosorbent assays (ELISA). Serum glucose was measured via a glucose-oxidase method, and serum insulin via radioimmunoassay. In each patient, IR was appraised via the Homeostasis Model Assessment (HOMA-IR) and the quantitative insulin sensitivity check index (QUICKI). Our results showed that at 7, 14 and 21 days post-treatment, with respect to the control group the two NPWT-treated groups had (i) significantly (p < 0.05) lower serum levels of TNF-α, IL-2 and IL-6; (ii) a significantly (p < 0.01) greater reduction in IR; and (iii) significantly (p < 0.05) higher wound healing rates. Moreover, at 14- and 21-days post-treatment, the NPWT+insulin-treated group had a significantly (p < 0.05) higher wound healing rate than the NPWT alone-treated group. In conclusion, the combined NPWT+insulin instillation treatment gave superior results by improving wound healing, decreasing inflammation at the wounds surface, and reducing IR in diabetic patients with infected DFUs.

The paradox of negative pressure wound therapy – in vitro studies

2010 ◽  
Vol 63 (1) ◽  
pp. 174-179 ◽  
Author(s):  
Nicolas Kairinos ◽  
Michael Solomons ◽  
Donald A. Hudson
Keyword(s):  
Negative Pressure ◽  
Negative Pressure Wound Therapy ◽  
In Vitro Studies ◽  
Wound Therapy

The effect of negative pressure wound therapy with antibacterial dressings or antiseptics on an in vitro wound model

2017 ◽  
Vol 26 (5) ◽  
pp. 236-242 ◽  
Author(s):  
J. Matiasek ◽  
K.J. Domig ◽  
G. Djedovic ◽  
R. Babeluk ◽  
O. Assadian
Keyword(s):  
Negative Pressure ◽  
Negative Pressure Wound Therapy ◽  
Wound Therapy ◽  
Wound Model

Application of a drainage film reduces fibroblast ingrowth into large-pored polyurethane foam during negative-pressure wound therapy in an in vitro model

2013 ◽  
Vol 21 (5) ◽  
pp. 697-703 ◽  
Author(s):  
Cornelia Wiegand ◽  
Steffen Springer ◽  
Martin Abel ◽  
Falko Wesarg ◽  
Peter Ruth ◽  
...  
Keyword(s):  
Polyurethane Foam ◽  
Negative Pressure ◽  
In Vitro Model ◽  
Negative Pressure Wound Therapy ◽  
Wound Therapy ◽  
Vitro Model

scholarly journals The Effect of Negative Pressure Wound Therapy with and without Instillation on Mature Biofilms In Vitro

Materials ◽  
2018 ◽  
Vol 11 (5) ◽  
pp. 811 ◽  
Author(s):  
Shamaila Tahir ◽  
Matthew Malone ◽  
Honghua Hu ◽  
Anand Deva ◽  
Karen Vickery
Keyword(s):  
Negative Pressure ◽  
Negative Pressure Wound Therapy ◽  
Wound Therapy

scholarly journals Technical Support of Wound Healing Processes: Project Status

2019 ◽  
Vol 5 (1) ◽  
pp. 521-523
Author(s):  
Jacquelyn Dawn Parente ◽  
Knut Möller ◽  
Sabine Hensler ◽  
Claudia Kühlbach ◽  
Margareta M. Mueller ◽  
...  
Keyword(s):  
Wound Healing ◽  
Mathematical Models ◽  
Negative Pressure ◽  
Negative Pressure Wound Therapy ◽  
Technical Support ◽  
Wound Therapy ◽  
Wound Area ◽  
Modal Treatment ◽  
Healing Processes

AbstractThe optimized wound healing (OWID) project provides technical support of wound healing processes. Advanced biophysical treatment therapies using light (photobiomodulation), negative pressure wound therapy (NPWT), and electrical stimulation show biological effects. Specifically, a biphasic dose-response curve is observed where lower doses activate cells, while above a threshold, higher doses are inhibitory. However, no standard protocols and no multi-modal treatment studies determine specific therapy needs. The OWID project aims to develop a multi-modal treatment device and modelbased therapy for individualized wound healing. This work presents the OWID project status. Currently, a photobiomodulation prototype delivers red, green, and blue light ‘medicine’ at prescribed therapeutic ‘doses’. The calculation of incident light necessarily considers transmission properties of the intervening cell culture plate. Negative pressure wound therapy (NPWT) and electrical impedance tomography (EIT) hardware are being adapted for use in vitro. Development of mathematical models of wound healing and therapy control are supported by treatment experiment outcome measures conducted in a wounded 3D tissue model. Parameter sensitivity analysis conducted on an existing mathematical model of reepithelialization results in changing parameter values influencing cellular movement rates. Thus, the model is robust to fit model parameters to observed reepithelialization rates under treatment conditions impacting cellular activation, inhibition, and untreated controls. Developed image analysis techniques have not captured changes in wound area after photobiomodulation treatment experiments. Alternatively, EIT will be tested for wound area analysis. Additionally, live dyes will be introduced to non-invasively visualize the reepithelialization front on a smaller, cellular scale. Finally, an overall therapeutic feedback control model uses model reference adaptive control to incorporate the intrinsic biological reepithelialization mechanism, treatment loops, and treatment controller modulation at a wound state. Currently, the OWID project conducts photobiomodulation treatment experiments in vitro and has developed mathematical models. Future work includes the incorporation of multi-modal wound healing treatment experiments.

scholarly journals Combining Negative Pressure Wound Therapy with Insulin Instillation Reduces Insulin Resistance and Accelerates Healing in Patients with Chronically Infected Wounds

2020 ◽  
Vol 7 (3) ◽  
pp. 1-7
Author(s):  
Xin-long Chen
Keyword(s):  
Insulin Resistance ◽  
Wound Healing ◽  
Negative Pressure ◽  
Negative Pressure Wound Therapy ◽  
Interleukin 2 ◽  
Serum Levels ◽  
Model Assessment ◽  
Wound Therapy ◽  
Tnf Α ◽  
Infected Wounds

Aim: This study aimed at evaluating the effects of negative pressure wound therapy (NPWT) combined with insulin instillation on wound healing and insulin resistance (IR) in patients with chronically infected wounds. Methods: Sixty-three patients were recruited and randomly divided into three groups (21 cases each) according to treatments: NPWT only; NPWT+insulin; and controls (conventional dressings). Both NPWT treatments lasted for three weeks. Thereafter, wounds’ healing conditions were assessed. At 1 day before and 7, 14, and 21 days posttreatment Interleukin- 6 (IL-6), Interleukin- 2 (IL-2), and Tumor Necrosis Factor-α (TNF-α) serum levels were assessed via specific double-antibody sandwich enzyme-linked immunosorbent assays; and plasma glucose and insulin were quantified via glucose-oxidase and radioimmunoassay, respectively. IR was appraised via homeostasis model assessment (HOMA-IR) and quantitative insulin sensitivity check index (QUICKI). Results: At 7, 14, and 21 days after treatments onset the two NPWT groups had significantly (P = 0.0001) lower IL-6, IL-2, and TNF-α serum levels and HOMA-IR values than controls while wound healing rates were higher (P < 0.001) than controls. Notably, the NPWT+insulin treatment results were better (P < 0.01) than those of NPWT alone. Conclusion: NPWT+insulin instillation improved wound healing, mitigated inflammation, and reduced IR in patients with chronically infected wounds.

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