scholarly journals VTC4 Polyphosphate Polymerase Knockout Increases Stress Resistance of Saccharomyces cerevisiae Cells

Biology ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 487
Author(s):  
Alexander Tomashevsky ◽  
Ekaterina Kulakovskaya ◽  
Ludmila Trilisenko ◽  
Ivan V. Kulakovskiy ◽  
Tatiana Kulakovskaya ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Heavy Metal ◽  
Stress Response ◽  
Stress Resistance ◽  
Alkaline Stress ◽  
Inorganic Polyphosphate ◽  
Divalent Metal Transporter ◽  
Metal Transporter ◽  
Elevated Expression ◽  
Chaperone Complex

Inorganic polyphosphate (polyP) is an important factor of alkaline, heavy metal, and oxidative stress resistance in microbial cells. In yeast, polyP is synthesized by Vtc4, a subunit of the vacuole transporter chaperone complex. Here, we report reduced but reliably detectable amounts of acid-soluble and acid-insoluble polyPs in the Δvtc4 strain of Saccharomyces cerevisiae, reaching 10% and 20% of the respective levels of the wild-type strain. The Δvtc4 strain has decreased resistance to alkaline stress but, unexpectedly, increased resistance to oxidation and heavy metal excess. We suggest that increased resistance is achieved through elevated expression of DDR2, which is implicated in stress response, and reduced expression of PHO84 encoding a phosphate and divalent metal transporter. The decreased Mg2+-dependent phosphate accumulation in Δvtc4 cells is consistent with reduced expression of PHO84. We discuss a possible role that polyP level plays in cellular signaling of stress response mobilization in yeast.

scholarly journals Role of VTC4 in Stress Response and Regulation of Inorganic Polyphosphate Levels in Yeast

2020 ◽  
Author(s):  
Alexander Tomashevsky ◽  
Ekaterina Kulakovskaya ◽  
Ludmila Trilisenko ◽  
Tatiana Kulakovskaya ◽  
Alexey Fedorov ◽  
...  
Keyword(s):  
Stress Response ◽  
Stress Tolerance ◽  
Stress Responses ◽  
Phosphate Uptake ◽  
Short Chain ◽  
Alkaline Stress ◽  
Inorganic Polyphosphate ◽  
A Subunit ◽  
Related Stress

Inorganic polyphosphate (polyP) is an important factor of stress tolerance in microbial cells. In yeast, the major enzyme of polyP biosynthesis is Vtc4, a subunit of the vacuole transporter chaperone (VTC) complex. In this study, we demonstrated that Vtc4 knockout in Saccharomyces cerevisiae not only decreased polyP content but also caused shifts in the composition of the intracellular polyP pool and changed the stress tolerance profile. In the mutant S. cerevisiae, the level of short-chain acid-soluble polyPs was decreased nearly 10-fold, whereas that of longer acid-insoluble polyPs was decreased only 2-fold, suggesting the existence of other enzymes compensating the production of long-chain polyPs. The Δvtc4 mutant showed inhibition of Mg2+-dependent phosphate uptake and decreased resistance to alkaline stress but increased tolerance to oxidation and heavy metal ions, especially Mn2+. Quantitative PCR revealed the upregulation of the DDR2 gene implicated in multiple stress responses and downregulation of PHO84 encoding a phosphate and Mn2+ transporter, which could account for the effects on phosphate uptake and Mn2+-related stress response in the Δvtc4 mutant. Our study indicates that short-chain polyPs, plays an important role in the regulation of stress response in yeast.

Stress resistance in Saccharomyces cerevisiae is strongly correlated with assembly of a novel type of multiubiquitin chain

1994 ◽  
Vol 14 (12) ◽  
pp. 7876-7883 ◽  
Author(s):  
T Arnason ◽  
M J Ellison
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Stress Response ◽  
Stress Resistance ◽  
Covalent Attachment ◽  
Strongly Correlated ◽  
Selective Degradation ◽  
Conjugating Enzymes

The covalent attachment of ubiquitin (Ub) to short-lived or damaged proteins is believed to be the signal that initiates their selective degradation. In several cases, it has been shown that the proteolytic signal takes the form of a multi-Ub chain in which successive Ub molecules are linked tandemly at lysine 48 (K-48). Here we show that Ub molecules can be linked together in vivo at two other lysine positions, lysine 29 (K-29) and lysine 63 (K-63). The formation of these alternative linkages is strongly dependent on the presence of the stress-related Ub conjugating enzymes UBC4 and UBC5. Furthermore, expression of Ub carrying a K-63 to arginine 63 substitution in a strain of Saccharomyces cerevisiae that is missing the poly-Ub gene, UBI4, fails to compensate for the stress defects associated with these cells. Taken together, these results suggest that the formation of multi-Ub chains involving K-63 linkages plays an important role in the yeast stress response. In broader terms, these results also suggest that Ub is a versatile signal in which different Ub chain configurations are used for different functions.

Lung injury after ozone exposure is iron dependent

2007 ◽  
Vol 292 (1) ◽  
pp. L134-L143 ◽  
Author(s):  
Andrew J. Ghio ◽  
Jennifer L. Turi ◽  
Michael C. Madden ◽  
Lisa A. Dailey ◽  
Judy D. Richards ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lung Injury ◽  
Iron Homeostasis ◽  
Biological Effects ◽  
Ozone Exposure ◽  
Divalent Metal Transporter 1 ◽  
Hek 293 ◽  
Divalent Metal Transporter ◽  
Metal Transporter ◽  
Elevated Expression

We tested the hypothesis that oxidative stress and biological effect after ozone (O3) exposure are dependent on changes in iron homeostasis. After O3 exposure, healthy volunteers demonstrated increased lavage concentrations of iron, transferrin, lactoferrin, and ferritin. In normal rats, alterations of iron metabolism after O3 exposure were immediate and preceded the inflammatory influx. To test for participation of this disruption in iron homeostasis in lung injury following O3 inhalation, we exposed Belgrade rats, which are functionally deficient in divalent metal transporter 1 (DMT1) as a means of iron uptake, and controls to O3. Iron homeostasis was disrupted to a greater extent and the extent of injury was greater in Belgrade rats than in control rats. Nonheme iron and ferritin concentrations were higher in human bronchial epithelial (HBE) cells exposed to O3 than in HBE cells exposed to filtered air. Aldehyde generation and IL-8 release by the HBE cells was also elevated following O3 exposure. Human embryonic kidney (HEK 293) cells with elevated expression of a DMT1 construct were exposed to filtered air and O3. With exposure to O3, elevated DMT1 expression diminished oxidative stress (i.e., aldehyde generation) and IL-8 release. We conclude that iron participates critically in the oxidative stress and biological effects after O3 exposure.

Diauxic shift-induced stress resistance against hydroperoxides in Saccharomyces cerevisiae is not an adaptive stress response and does not depend on functional mitochondria

2001 ◽  
Vol 39 (3) ◽  
pp. 137-149 ◽  
Author(s):  
Angel F. Maris ◽  
André L.K. Assumpção ◽  
Diego Bonatto ◽  
Martin Brendel ◽  
João Antônio P. Henriques
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Stress Response ◽  
Stress Resistance ◽  
Diauxic Shift ◽  
Induced Stress ◽  
Adaptive Stress Response

Engineering expression of the heavy metal transporter MerC in Saccharomyces cerevisiae for increased cadmium accumulation

2009 ◽  
Vol 86 (2) ◽  
pp. 753-759 ◽  
Author(s):  
Masako Kiyono ◽  
Kiyomi Miyahara ◽  
Yuka Sone ◽  
Hidemitsu Pan-Hou ◽  
Shimpei Uraguchi ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Heavy Metal ◽  
Cadmium Accumulation ◽  
Metal Transporter

scholarly journals Stress resistance in Saccharomyces cerevisiae is strongly correlated with assembly of a novel type of multiubiquitin chain.

1994 ◽  
Vol 14 (12) ◽  
pp. 7876-7883 ◽  
Author(s):  
T Arnason ◽  
M J Ellison
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Stress Response ◽  
Stress Resistance ◽  
Covalent Attachment ◽  
Strongly Correlated ◽  
Selective Degradation ◽  
Conjugating Enzymes

The covalent attachment of ubiquitin (Ub) to short-lived or damaged proteins is believed to be the signal that initiates their selective degradation. In several cases, it has been shown that the proteolytic signal takes the form of a multi-Ub chain in which successive Ub molecules are linked tandemly at lysine 48 (K-48). Here we show that Ub molecules can be linked together in vivo at two other lysine positions, lysine 29 (K-29) and lysine 63 (K-63). The formation of these alternative linkages is strongly dependent on the presence of the stress-related Ub conjugating enzymes UBC4 and UBC5. Furthermore, expression of Ub carrying a K-63 to arginine 63 substitution in a strain of Saccharomyces cerevisiae that is missing the poly-Ub gene, UBI4, fails to compensate for the stress defects associated with these cells. Taken together, these results suggest that the formation of multi-Ub chains involving K-63 linkages plays an important role in the yeast stress response. In broader terms, these results also suggest that Ub is a versatile signal in which different Ub chain configurations are used for different functions.

Hypoxia induces changes in expression of isoforms of the divalent metal transporter (DMT1) in rat pheochromocytoma (PC12) cells

2005 ◽  
Vol 69 (11) ◽  
pp. 1647-1655 ◽  
Author(s):  
Agnieszka Lis ◽  
Prasad N. Paradkar ◽  
Steve Singleton ◽  
Hung-Chieh Kuo ◽  
Michael D. Garrick ◽  
...  
Keyword(s):  
Pc12 Cells ◽  
Divalent Metal ◽  
Divalent Metal Transporter ◽  
Metal Transporter ◽  
Pheochromocytoma Pc12 ◽  
Pheochromocytoma Pc12 Cells

scholarly journals The Role of Aquaporin Overexpression in the Modulation of Transcription of Heavy Metal Transporters under Cadmium Treatment in Poplar

Plants ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 54
Author(s):  
Andrea Neri ◽  
Silvia Traversari ◽  
Andrea Andreucci ◽  
Alessandra Francini ◽  
Luca Sebastiani
Keyword(s):  
Heavy Metal ◽  
Transcriptional Response ◽  
Metal Transporters ◽  
Metal Transporter ◽  
Cadmium Treatment ◽  
Tolerance To Cadmium ◽  
Different Response ◽  
Cd Translocation ◽  
Hydroponic Conditions

Populus alba ‘Villafranca’ clone is well-known for its tolerance to cadmium (Cd). To determine the mechanisms of Cd tolerance of this species, wild-type (wt) plants were compared with transgenic plants over-expressing an aquaporin (aqua1, GenBank GQ918138). Plants were maintained in hydroponic conditions with Hoagland’s solution and treated with 10 µM of Cd, renewed every 5 d. The transcription levels of heavy metal transporter genes (PaHMA2, PaNRAMP1.3, PaNRAMP2, PaNRAMP3.1, PaNRAMP3.2, PaABCC9, and PaABCC13) were analyzed at 1, 7, and 60 d of treatment. Cd application did not induce visible toxicity symptoms in wt and aqua1 plants even after 2 months of treatment confirming the high tolerance of this poplar species to Cd. Most of the analyzed genes showed in wt plants a quick response in transcription at 1 d of treatment and an adaptation at 60 d. On the contrary, a lower transcriptional response was observed in aqua1 plants in concomitance with a higher Cd concentration in medial leaves. Moreover, PaHMA2 showed at 1 d an opposite trend within organs since it was up-regulated in root and stem of wt plants and in leaves of aqua1 plants. In summary, aqua1 overexpression in poplar improved Cd translocation suggesting a lower Cd sensitivity of aqua1 plants. This different response might be due to a different transcription of PaNRAMP3 genes that were more transcribed in wt line because of the importance of this gene in Cd compartmentalization.

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